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研究生:沈毓盛
研究生(外文):Yu-Sheng Shen
論文名稱:亞磷酸根及亞磷酸根利用菌對小白菜Brassica chinensis L. Cultiva生長的影響
論文名稱(外文):Effect of phosphite and phosphite-utilizing microorganisms on the growth of Pak-choi (Brassica chinensis L. Cultiva)
指導教授:黃裕銘
口試委員:陳仁炫譚鎮中鍾仁賜簡宣裕
口試日期:2011-07-19
學位類別:碩士
校院名稱:國立中興大學
系所名稱:土壤環境科學系所
學門:農業科學學門
學類:農業化學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:91
中文關鍵詞:亞磷酸
外文關鍵詞:phosphite
相關次數:
  • 被引用被引用:1
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亞磷酸為一種還原性磷,其結構與磷酸相似,不同之處磷酸為三質子酸亞磷酸為二質子酸。亞磷酸鹽目前已被做為某些病害防治劑,但是也發現對某些作物生長有抑制現象甚至造成生態不平衡。本研究利用水耕探討亞磷酸根和磷酸根濃度比對小白菜生長及其硝酸還原酶及磷酸酶活性的影響。並且從土壤篩選可利用亞磷酸根微生物,接種至水耕系統中,觀察其對亞磷酸根養液小白菜生長的影響。
本試驗結果顯示,亞磷酸根相對濃度提高時小白菜生長量無論地上部及根鮮重及乾重皆降低。對植體總磷,抽出性磷酸根、鎂的濃度及葉綠素測值也有降低趨勢,甚至達顯著水準,植體硝酸根濃度則有提高趨勢且達顯著水準。降低葉片硝酸還原酵素及磷酸酵素的活性達顯著水。但是全施亞磷酸根小白菜莖葉產量比不施磷處理高,鮮重增加69 %,乾重增加9 %;根部鮮重降低56 %,乾重降低67 %。添加所篩選微生物處理莖葉及根鮮重分別提高37~93 %及27~62 %。


Phosphite (HPO3-2, Phi) is a reduced form of phosphorus, having a similar structure to phosphate (PO4-3, Pi). The former is a diprotic acid, but phosphate is a triprotic acid. Phosphite salts have been used as a fungicide for some diseases control. Some plants can used it as phosphorus fertilizer, but no phosphorus fertilizer effect for other plants, after the long-term use it even caused ecological problem. This study using hydroponic system to investigate the effect of phosphite/phosphate ratio in the nutrient solution on the growth, nitrate reductase, phosphatase, nutrient uptake of Pak-choi (Brassica chinensis L.,Cultiva). Screening Phi using bacteria from soil and inoculated into nutrient solution to evaluate their effect on the growth of Pak-choi.
Results showed that increased the phosphite/phosphate ratio in nutrient solution decreased weights of shoot and root in fresh and dry, concentrations of extractable phosphate, total phosphorus, and magnesium, activities of leaf nitrate reductase and phosphatase, and the chlorophyll index. Evidence showed phosphate contributes a partial function as phosphorus fertilizer. Although the fresh weight and dry weight of Pak-choi root of 100% phosphate treatment was 56 %, and 67 % decreased, respectively, compared to no phosphorus treatment. The fresh weight and dry weight of Pak-choi shoot was 69 % and 9 % increased, respectively, compared to the no phosphorus treatment.
The inoculation of the top three most effective phosphate using bacteria, increased the Pak-choi shoot and root fresh weights by 37-93 % and 27-62 %, respectively, compared to no inoculation treatment

摘要 i
Abstract ii
目錄 IV
表次 VII
圖次 IX
附錄 X
壹、前言 1
貳、前人研究 3
一、磷酸與亞磷酸介紹 3
二、亞磷酸應用於作物生物防治 7
三、亞磷酸之運輸轉化 15
四、亞磷酸營養方面: 18
(一) 亞磷酸利用於水耕栽培作物: 18
(二) 支持亞磷酸可供應磷肥之論點: 19
(三) 不支持亞磷酸可供應磷肥論點: 20
(四) 植物缺磷之徵狀: 20
(五) 亞磷酸對於植物體內磷酸充足與否之影響: 21
(六) 植物磷逆境下之機制: 23
(七) 植體內氮磷之間相互影響: 23
五、亞磷酸測定方面: 25
参、材料與方法 27
一、試驗處理介紹 27
二、水耕試驗製備 30
三、分析方法 34
(一)、植體P、K、Ca、Mg、Fe、Mn、Cu、Zn和Mo含量 34
(二)、植體全氮量測定 35
(三)、植體陰離子含量測定 35
(四)、植物硝酸還原酶活性 (nitrate reductase activity, NRA) 測定 37
(五)、葉片葉綠素測定: 38
(六)、植物磷酸酯水解酶活性 (acid phosphatase) 測定 39
四、試驗設計及統計 40
肆、結果與討論 41
一、小白菜生長在含不同比例磷酸與亞磷酸水耕養液中之生長情形 41
二、小白菜生長在50% Pi + 50% Phi比例中並接種不同微生物生長情形 55
三、微生物接種於含不同比例磷酸亞磷酸水耕養液對小白菜生長影響 60
四、探討微生物並未明顯幫助小白菜在亞磷酸養液中生長之情況 65
伍、結論 69
陸、參考文獻 71



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