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研究生:余信翰
研究生(外文):Hsin-Han Yu
論文名稱:條滸苔萃取物之生理活性探討
論文名稱(外文):Studies on physiological activity of Ulva clathrata extractives
指導教授:曹欽玉
指導教授(外文):Ching-Yu Tsao
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:食品科學系
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2010
畢業學年度:99
語文別:中文
論文頁數:79
中文關鍵詞:條滸苔
相關次數:
  • 被引用被引用:1
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本研究之目的主要探討條滸苔Ulva clathrata 水溶性萃取物的抗氧化活性。將原料加入100倍的去離子水在100oC下加熱1小時能萃取出最大量的水溶性多醣。此熱水萃取物分別經過酸鹼處理或加入4倍體積的乙醇(95%)進行沉澱。前述各組經過酵素水解後,測定其組成分和抗氧化活性。結果顯示,各組之螯合亞鐵離子能力、清除DPPH 自由基能力、Trolox 抗氧化當量及還原能力,皆隨著濃度的增加有提升的趨勢。酸鹼處理組比熱水處理組有較強的還原能力和熬合亞鐵離子能力。各萃取物經α-amylase和cellulase處理後,以cellulase處理會有較多的還原醣含量,結果也以cellulase處理之組別有較強之抗氧化能力,由此可知,Ulva clathrata 水溶性萃取物之抗氧活性可能由於增加藻類多醣的降解,而進一步增強抗氧化能力。
The purpose of this research was to clarify the antioxidant activity of Ulva clathrata water soluble extractives. The maximal polysaccharides contents were extracted by adding one hundred volumes of de-ionized water to original algae weight, and heating at 100oC for 1 hour. The hot-water extractives by acid and base treatment or precipitation used adding four volumes of 95% ethanol. Each extractive from Ulva clathrata was hydrolyzed by enzyme. The tentative compositions and antioxidant activities of extractives were determined, respectively. According to results, it show that reducing power, chelating Fe2+ effect, scavenging of DPPH and Trolox equivalent antioxidant capacity of each extractive were increase as concentration increase. After acid and base treating, the reducing power and chelating Fe2+ effect of extractives were better than hot-water extract only. Each extractive from Ulva clathrata hydrolyzing with α-amylase or cellulase, it showed the reducing sugar content of later was more than former; and the antioxidant activity of cellulase treatment group exhibited the best among other groups. Therefore it supposed that the antioxidant activity of water soluble extractives of Ulva clathrata was offered by degradation of its polysaccharides.
中文摘要.........................I
英文摘要.........................II
目錄...........................III
壹、前言.........................1
貳、文獻整理.......................2
一、滸苔的簡介....................2
二、滸苔的醫用價值..................2
三、條滸苔的簡介...................3
四、條滸苔的應用...................5
五、條滸苔之多醣體..................6
六、α-Amylase簡介..................8
七、Cellulase簡介...................9
八、海藻多醣與抗氧化.................9
九、脂質氧化探討..................10
参、材料與方法......................16
一、實驗材料....................16
二、實驗方法....................18
肆、結果與討論......................31
一、條滸苔萃取條件.................31
二、條滸苔萃取物之組成份分析............33
三、條滸苔萃取物之抗氧化能力探討..........35
四、條滸苔各種萃取物之抗氧化綜合性比較.......40
伍、結論.........................42
陸、參考文獻.......................43
柒、圖表.........................49

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