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研究生:邱詠評
研究生(外文):Ciou, Yung-Ping
論文名稱:嗜高溫放線菌 Thermobifida fusca 所分泌熱安定性阿魏酸酯酶之生產與應用於木質纖維素分解之研究
論文名稱(外文):Production of Thermostable Ferulic Acid Esterase from Thermobifida fusca and Its Application in Lignocellulose Degradation
指導教授:楊昭順黃煜珺
指導教授(外文):Yang, Chao-HsunHuang, Yu-Chun
口試委員:林維炤張聰民蔡玫琳楊昭順黃煜珺
口試委員(外文):Lin, Wei-ChoChang, Tsong-MinTsai, Mei-LinYang, Chao-HsunHuang, Yu-Chun
口試日期:2011-07-06
學位類別:碩士
校院名稱:靜宜大學
系所名稱:化粧品科學系
學門:民生學門
學類:美容學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:89
中文關鍵詞:阿魏酸酯酶嗜高溫放線菌
外文關鍵詞:ferulic acid esteraseThermobifida fusca
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中文摘要 ..................................................................................................................... I
Abstract ...................................................................................................................... III
目錄 .............................................................................................................................. V
表次 ............................................................................................................................ VII
圖次 .......................................................................................................................... VIII
第一章 緒言 .............................................................................................................. 1
第一節 嗜高溫放線菌 (Thermophilic actinomycetes) ..................................... 1
第二節 阿魏酸酯酶 (ferulic acid esterase) ......................................................... 5
第三節 阿魏酸 (ferulic acid) ................................................................................ 13
第四節 研究動機與架構 ...................................................................................... 15
第二章 篩選具生產阿魏酸酯酶能力之嗜高溫放線菌 ....................... 17
一、 前言 .................................................................................................................. 17
二、 材料與方法 .................................................................................................... 17
三、 結果 .................................................................................................................. 20
(一) 具阿魏酸酯酶活性之嗜高溫放線菌菌株篩選 ................................... 20
(二) No. 2-2 及No. 13-13 菌株之菌種鑑定 ..................................................... 22
四、 討論 .................................................................................................................. 31
第三章 利用Thermobifida fusca 生產阿魏酸酯酶及其酵素性質之研
究 ................................................................................................................................. 32
一、 前言 .................................................................................................................. 32
二、 材料與方法 .................................................................................................... 32
三、 結果 .................................................................................................................. 47
(一) 不同碳源對阿魏酸酯酶活性表現之影響 ............................................. 47
(二) 酯酶最適生長培養基之探討 ..................................................................... 49
(三) 酵素共同作用分解木質纖維素 ................................................................ 56
(四) T. fusca NTU 22 所分泌阿魏酸酯酶之純化 ......................................... 59
(五) T. fusca NTU 22 所分泌阿魏酸酯酶之性質 ......................................... 65
四、 討論 .................................................................................................................. 75
第四章 結論 ........................................................................................................... 77
參考文獻 .................................................................................................................. 79
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