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研究生:郭益華
研究生(外文):I-Hua Kuo
論文名稱:非洲白蔘對老化促進小鼠學習記憶力、生殖功能及抗氧化狀態之影響
論文名稱(外文):Effect of Mondia whitei on learning and memory ability、reproductive performances and antioxidative status in senescence accelerated mice
指導教授:王銘富王銘富引用關係
指導教授(外文):Ming-Fu Wang
學位類別:碩士
校院名稱:靜宜大學
系所名稱:食品營養研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:131
中文關鍵詞:非洲白蔘老化促進小鼠抗氧化生殖功能
外文關鍵詞:antioxidantsenescence-accelerated mouseMondia whiteireproductive performances
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非洲白蔘(Mondia Whitei)根部具有提升雄性大鼠生殖功能之效果,但其對學習記憶能力及抗氧化之影響並不明確。本研究目的在探討非洲白蔘對雄性老化促進小鼠(senescence-accelerated mouse)學習記憶能力、生殖功能及抗老化之影響。實驗以3月齡雄性老化促進小鼠為對象,隨機分為控制組、非洲白蔘組及一、二、三號配方組,共5組。非洲白蔘組含非洲白蔘(1 g /ml),一號配方組含非洲白蔘(1 g /ml)、淫羊藿(0.5 g /ml)、仙茅(0.25 g /ml)、紅棗(0.83 g /ml),二號配方組含非洲白蔘(1 g /ml)、淫羊藿(0.5 g /ml)、巴戟天(0.41 g /ml)、紅棗(0.83 g /ml),三號配方組含非洲白蔘(1 g /ml)、杜仲(0.66 g /ml)、黃耆(1.5 g /ml)、紅棗(0.83 g /ml)。連續管灌8週(劑量:0.3ml浸膏/mouse/day),每組6隻。實驗期間紀錄攝食量、飲水量及體重變化。於第7週做老化指數評估(aging score),以活動量試驗(Open field activity test)來測其行為;主動迴避試驗(active shuttle avoidance test)及單次被動迴避試驗(single-trial passive avoidance test)來評估其學習記憶能力。犧牲後進行血液生化分析、取肝臟檢測肝中超氧歧化酶(SOD)、觸酶(Catalase)、麩胱苷肽過氧化酶(GPx)及丙二醛(MDA);並評估精子活動力(Motility)、濃度(Concentration)及畸形率(Abnormality)之結果。另以免疫組織化學染色觀察腦部類澱粉蛋白(β-amyloid protein, Aβ)沉積情況。
結果顯示,實驗期間體重變化、攝食量、飲水量及活動量均無顯著差異(P > 0.05)。在老化指數評估方面,非洲白蔘及其複方之老化指數總分皆顯著低於控制組 (P < 0.05)。在學習記憶力方面,非洲白蔘組及其複方於主動迴避試驗實驗及單次被動迴避試驗皆較控制組有顯著之記憶保留及較高之逃避次數(P < 0.05)。在生殖功能方面,非洲白蔘組及其複方組與控制組相比,精子活動力及濃度皆有增強之功效(P < 0.05),但對精子畸形率則無顯著差異(P > 0.05)。在抗氧化方面,非洲白蔘及其複方的給予能顯著提升SAMP8小鼠肝臟中SOD、Catalase、GPx 及MDA活性濃度(P < 0.05)。在β-類澱粉蛋白方面,非洲白蔘及其複方能降低腦部之沉積(P < 0.05)。綜合以上結果,非洲白蔘及其複方能提升老化促進小鼠體內抗氧化防禦系統能力、減少氧化壓力之傷害、減少腦部β-類澱粉蛋白沉積、改善小鼠之學習記憶能力及增強生殖功能。
Mondia White roots have elevated reproductive performance in male rats, but effect of it is indeterminate on learning and memory ability and antioxidative status.The purpose of this study was to investigate the effect of Mondia White on learning and memory abilityreproductive performances and antioxidative status in senescence accelerated mice.Three-month-old SAMP8 male were used in this study.SAMP8 mice were randomly into five groups, where each group contained six mice, including control group, Mondia whitei group (Mondia whitei 1 g /ml), First formula (Mondia whitei 1 g /ml、Epimedium 0.5 g /ml、Common Curculigo 0.25 g /ml、Zizyphus jujuba Mill 0.83 g /ml), Second formula (Mondia whitei 1 g /ml、Epimedium 0.5 g /ml、Morinda officinalis How 0.41 g /ml、Zizyphus jujuba Mill 0.83 g /ml), Third formula (Mondia whitei 1 g /ml、Eucommia ulmoides Oliv. 0.66 g /ml、Astragalus huantchy Franchet 1.5 g /ml、Zizyphus jujuba Mill 0.83 g /ml).This study was at different diets (0.3ml extract/mouse/day) by gastric gavage consecutively for 7 weeks. Food intake, water drink and body weight was also measured during experimental period. One week before scarification, the aging scores were measured, Open field activity test was recorded in evaluation for behavior, and active shuttle avoidance test and single-trial passive avoidance test were performed independently. The mice were sacrificed to analyze the biochemical parameters of sera. The superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase(GPx) and malondialdehyde (MDA) concentration activities of liver were measured. The motility, concentration and abnormality of spermatozoa were also evaluated. β-amyloid protein (Aβ) deposition of brain were evaluated. The results showed that there was no significant difference (P &gt; 0.05) in body weight, food intake, water drink and locomotion among each group. The aging score of Mondia whitei group and its compound groups were significant lower than control group (P &lt; 0.05). Results from active shuttle avoidance test and single-trial passive avoidance test showed that Mondia whitei group and its compound groups had significant better learning memory ability (P &lt; 0.05).
The Mondia whitei group and its compound groups of concentration and motility of spermatozoa were higher control group (P &lt; 0.05), but there no significant difference (P &gt; 0.05) in spermatozoa abnormality. Activities of antioxidant ability, including SOD, Catalase, GPx, and MDA concentration, in the Mondia whitei group and its compound groups were significant higher than that of control group (P &lt; 0.05). Beside, the Aβ deposition of brain in Mondia whitei group and its compound groups were significant lower than control group (P &lt; 0.05). In summary, the supplement of Mondia whitei and its compound may promote the antioxidant defense system, reduce oxidative stress-induced damage, reduce the accumulation of β-amyloid deposition, improve learning and memory ability, and enhanced reproductive performances.
中文摘要...................................................I
英文摘要.................................................III
目錄......................................................VI
表目錄...................................................XII
圖目錄..................................................XIII

第一章 前言...............................................1
第二章 文獻回顧............................................5
第一節 非洲白蔘.......................................5
壹、非洲白蔘簡介....................................5
貳、非洲白蔘活性成分、營養成分及藥理作用...............6
參、非洲白蔘安全性評估...............................9
第二節 非洲白蔘複方...................................10
壹、淫羊藿.........................................10
貳、仙茅..........................................12
参、巴戟天.........................................14
肆、杜仲葉.........................................15
伍、黃耆..........................................18
陸、紅棗..........................................20
第三節 自由基、老化及氧化防禦系統........................23
壹、自由基與活性氧物質之介紹.........................23
一、自由基與活性氧物質..........................23
二、自由基及活性氧物質之來源.....................24
貳、氧化壓力與人類疾病之關係.........................24
參、氧化壓力與老化之相關性...........................25
肆、體內抗氧化防禦系統..............................26
一、酵素型抗氧化劑...............................27
二、非酵素型抗氧化劑.............................28
第四節 阿茲海默症......................................31
壹、阿茲海默症的發現歷程............................31
貳、阿茲海默症的症狀................................32
參、阿茲海默症病因 .................................32
一、澱粉樣蛋白..................................32
二、t-蛋白.....................................33
三、乙醯膽鹼....................................34
肆、阿茲海默與氧化壓力..............................35
第五節 男性不孕症......................................37
壹、男性不孕的原因 37
一、先天性染色體的異常...........................37
二、內分泌系統的異常.............................38
三、睪丸功能的異常...............................38
四、精子功能的異常...............................38
貳、氧化壓力與精子功能..............................39
參、反應性氧化物質與精子活動力.......................40
第六節 老化促進小鼠....................................42
壹、老化促進小鼠來源................................42
貳、老化促進小鼠之老化特徵抗氧化狀態..................42
參、老化促進小鼠之病理特徵...........................43
肆、老化促進小鼠與學習記憶...........................44
第三章 材料與方法..........................................46
第一節 實驗動物......................................46
第二節 實驗材料......................................47
第三節 實驗劑量......................................49
第四節 實驗方法與步驟.................................50
壹、實驗流程.......................................50
貳、活動量測試.....................................52
參、老化指數評估...................................53
肆、學習記憶測試...................................57
一、主動迴避試驗...............................57
二、單次被動迴避試驗............................58
伍、血液生化學分析..................................60
陸、生殖功能.......................................60
一、儀器設備...................................60
二、試藥配製...................................60
三、精子活動力之評估............................61
四、精子濃度之評估..............................61
五、精子畸形率之評估............................61
柒、肝臟抗氧化能力分析..............................63
一、超氧歧化酶.................................64
二、觸酶......................................67
三、麩胱苷肽過氧化酶............................68
四、丙二醛.....................................70
捌、腦部病理切片觀察...............................72
第五節 統計分析.....................................80
第四章 結果與討論..........................................81
第一節 體重、攝食量及攝水量...........................81
第二節 活動量.......................................81
第三節 老化指數.....................................84
第四節 學習與記憶能力................................86
壹、主動迴避試驗.................................86
貳、被動迴避試驗.................................88
第五節 器官重量.....................................90
第六節 血液生化學分析................................92
第七節 精子生殖功能評估..............................94
壹、精子活動力...................................94
貳、精子濃度.....................................96
參、精子畸形率...................................98
第八節 抗氧化酵素活性與過氧化物......................100
壹、超氧歧化酶..................................100
貳、觸酶.......................................102
參、麩胱苷肽過氧化酶.............................104
肆、丙二醛......................................106
第九節 腦部β-類澱粉蛋白.............................108
第五章 總論..............................................112
第六章 參考文獻..........................................115
卞以潔、胡雅兒、夏宗勤、易寧育、趙勝利,1988。中藥藥理與臨床4(3):29
王靖雅,2009。北冬蟲夏草對老化促進小鼠抗氧化狀態及學習記憶之探討。靜宜大學食品營養系碩士論文
行政院經濟建設委員會,2010。2010年至2060年台灣人口推計。
行政院主計處內政部,2011。民國99年底人口結構分析。
付嘉、熊彬、陳峰、王建杰、張濤、羅文哲,2005。巴戟天對老齡小鼠免疫功能的影響。中國老年學雜志 25: 312-313
李炳如、佘運初,1984。中醫雜誌 25(7):543
吳瓊、程小衛、雷光青、陳士明、陳家寬、周銅水,2007。仙茅苷對自由基的清除作用。中國現代應用藥學 24(1):6-9
高本初,1981。新編中藥大辭典。新文豐出版社。
徐美如,2005。中草藥水萃物抗氧化及誘導肝癌細胞凋亡之研究。國立中興大學食品科學系碩士論文。
徐雯敏,2004。香椿對老化促進小白鼠學習記憶能力、抗氧化狀態與精子品質之影響。靜宜大學碩士論文。
郭美璋,2000。男性不孕症患者之生育概念、不孕療程憂慮及其焦慮狀態及治療選項接受度之相關研究。臺北醫學院醫學研究所碩士論文。
郭肇凱、 張隆仁、 陳榮五、廖俊旺,2006。新引進藥用植物VUKA (Mondiawhitei L.)對小屬餵飼之安全性評估。台中區農業改良場研究彙報 91:21-29
郭肇凱、廖俊旺、張隆仁、 陳榮五,2007。新引進藥用植物Mondia whitei L. (VUKA) 水萃取物連續餵飼天之生理安全及雄性生殖功能 評估。台中區農業改良場研究
彙報 95:21-29
陳莉惠,2003。Aβ集中草藥對APP代謝之影響及其機制之探討。陽明大學生物醫藥研究所碩士論文。
黃瑞東,1996。黃耆成分及藥理作用。pharmacognosy. ROC. 4:34-44
張梅、宋芹、郭平,2007。仙茅補腎壯陽有效成分的初步研究及仙茅素A的含量測定。華西藥學雜志 22:191-193
楊欣、張永華、丁彩飛、顏志中、杜靜,2006。巴戟天水提物對人精子膜功能氧化損傷的保護作用。中國中藥雜志 31(19):1614-1617
揚霄,2009。巴戟天多糖的降血糖和抗氧化作用研究。Journal of Chinese Medicinal Materials 32(6):949-951
劉匯波、邢善田,1990。中藥藥理與臨床 6(2):18
http://ezfun.coa.gov.tw/view.php?theme=news&id=N_sandra_20080325182700&city=N&class=N06&graph_idx=1
Adams DO, Hamilton TA. Macrophages as destructive cells in host defense. In: Gallin, J. I., Goldstein, I. M., Synderman, R., eds. Inflammation: basic principles and clinical correlates. New York: Raven 1992; 637-62.
Adjanohoun, E. C., N. Aboubakar, K. Dramane, M. E. Ebot, J. A., Ekpere and E. G. Enow-Orock. Traditional medicine and pharmacopoeia. Contribution to ethnobotanical and floristic studies in Cameroon. Lagos: OUA/STRC. 1996; 301 pp.
Aitken, J. & Fisher, H. Reactive oxygen species generation and human spermatozoa: the blance of benefit and risk. Bioessays 1994; 16: 259-267.
Aitken RJ, Clarkson JS, Fishel S. Generation of reactive oxygen species and, lipid
peroxidation, and human sperm function. Biol Reprod 1989; 41: 183-197.
Armstrong JS, Rajasekaran M, Chamulitrat W, Gatti P, Hellstrom WJ, Sikka SC.
Characterization of reactive oxygen species induced effects on human spermatozoamovement and energy metabolism. Free Radic Biol Med 1999; 26: 869-880.
Akiguchi I, Yagi H, Ueno M, Takemura M, Kitabayashi T, Seriu N, Kawamata T, Nakamura S, Shimada A, Takeda T. Age related morphological changes in the brain of senescence-accelerated mouse (SAMP8). In The SAM Model of Senescence, Proceeding of the First International Conference on Senescence.
1994; 25:67-72.
Alvarez, A., C. Opazo, R. Alarcon, J. Garrido, and N. C. Inestrosa. Acetylcholinesterase promotes the aggregation of amyloid-beta-peptide fragments by forming a complex with the growing fibrils: J Mol Biol, v. 1997; 272: 348-61.
Alvarez JG, Touchstone JC, Blasco L, Storey BT. Spontaneous lipid peroxidation and
production of hydrogen peroxide and superoxide in human spermatozoa: superoxoide dismutase as major enzyme protectant against oxygen toxicity. J Androl 1987; 8: 338-348.
Armstrong, J. S. Rajasekaran, M, Chamulitrat W, Gatti P, Hellstrom WJ, Sikka SC.
Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism. Free Radic Biol Med 1999; 26: 869-880.
Auger J, Kunstmann JM, Czyblik F, Jouannet P. Decline in semen quality among ertile men in Paris during the past 20 years. N Engl J Med 1995; 332: 281-285.
Behl, C., Davis J., Lesley, R. & Schbert, D. Vitamin E protects nerve cells from amyloid beta protein toxicity. Biochem. Biophys. Res. Commun. 1992; 186: 944-950.
Biologically active principles of crude drugs. Antidiabetic principles of corni fructus in experimental diabetes induced by streptozotocin (author''s transl). Yakugaku Zasshi 1981; 101:86-90.
Bonorden WR, Pariza MW. Antioxidant nutrients and protection from free radicals, In: Nutr. Toxicol, Kostsonis FN, Mackey M and Hjelle J ed. Raven press. New York. 1994; 19- 48.
Butterfield DA, Drake J, Pocernich C, Castegna A. Castegna A. Evidence of oxidative damage in Alzheimer''s disease brain: central role for amyloid beta-peptide. Trends Mol Med. 2001; 7:548-54.
Carlsen, A., Rice, E., Nicholas, A. Evidence for decreasing quality of semen during past 50 years. B. M. J. 1992; 305: 609-613.
Carro-Juarez M., Cervantes E., Cervantes-Mendez M., and Rodriguez-Manzo M. Aphrodisiac properties of Montanoa tomentosa aqueous crude extract in male rats. Pharmacol Biochem Behav 2004; 78: 129-134
Chen CS, Chao HT, Pan RL, Wei YH. Hydroxyl radical- induced decline in motility and increase in lipid peroxidation and DNA modification in human sperm. Biochem Mol Biol Int 1997; 43: 291-303.
Chen, R. & Wen, H. Clinical treatment of male infertility with Sheng jing pill. Int. J. Orient. Med. 1996; 21: 144-147.
Chien CT., Lee PH., Chen..F., Ma MC., Lai MK. and Hsu SM. De novo demonstration and co-localization of free-radical production and apoptosis formation in rat kidney subjected to ischemia reperfusion. J Am Soc Nephrol 2001; 12:973-982.
Church DF. and Pryor WA. Free radical chemistry of cigarette smoke and its
toxicological implications. Environ. Health perspect 1985; 64:111-126.
Chow CK. Vitamin E and oxidative stress. Free Rad Biol Med 1991; 11:215-32.
Cicero A.F.G., Bandieri E. and Arletti R. Lepidium meyenii Walp. improves sexual behaviour in male rats independently from its action on spontaneous locomotor activity. J Ethnopharmacol 2001; 75: 225-229.
Cicero A.F.G., Bandieri E. and Arletti R. Lepidium meyenii Walp. improves sexual behaviour in male rats independently from its action on spontaneous locomotor activity. J Ethnopharmacol 2001; 75: 225-229.
Crimmel AS, Conner CS, Monga M. Withered Yang: a review of traditional Chinese
medical treatment of male infertility and erectile dysfunction. J Androl 2001; 22:
173-182.
Collins JA. Male infertility: the interpretation of the diagnostic assessment. In: Mishell DR, Paulsen CA, Lobo RA, eds. The Year Book of Infertility. Chicago: Year Book Medical Publishers Inc 45, 1989.
Coyle JT, Puttfarcken P. Oxidative stress, glutamate, and neurodegenerative
disorders. Science. 1993; 262:689-95.
Cummins, J. M. Jequier, A. M & Kan, R. Molecular biology of human male infertility: links with aging, mitochondrial genetics, and oxidative stress? Mol. Reprod. Dev. 1944; 37: 345-362.
Delacourte, A., and A. Defossez, Alzheimer''s disease: Tau proteins, the promoting factors of microtubule assembly, are major components of paired helical filaments: J Neurol Sci, 1986; 76:173-86.
de Lamirande E, Gagnon C. Impact of reactive oxygen species on spermatozoa: a
balancing act between beneficial and detrimental effects. Hum Reprod ( suppl 1)
1995; 10:15-21.
de Lamirande E, Gagnon C. Reactive oxygen species and human spermatozoa. I. Effects on the motility of intact spermatozoa and on sperm axonemes. J Androl 1992; 13: 368-378.
de Lamirande E, Gagnon C. Reactive oxygen species (ROS) and reproduction. Adv Exp Med Biol 1994; 366: 185-197.
Elaine R, Peskind MD. Neurobiology of Alzheimer’s disease. J Clin Psychiatry 1996; 57: 5-8.
Ericsson SA, Garner DL, Thomas CA, Downing TW, Marshall CE. Intereslationships among fluorometric analyses of spermatozoa function, classical semen quality parameters and the fertility of frozen-thawed bovine spermatozoa. Theriogenology 1993; 39: 1009-1024.
Esfandiari N, Saleh RA, Blaut AP, Sharma RK, Nelson DR, Thomas AJ Jr, Falcone T,Agarwal A. Effects of temperature on sperm motion characteristics and reactive oxygen species. Int J Fertil Womens Med 2002; 47: 227-233.
Farr SA, Flood JF, Moley JE. The effect of cholinergic, GABAergic, serotonergic, and glutamatergic receptor modulation on posittrial memory processing in the hippocampus. Neurobiol Learn Mem 2000; 83: 173-7.
Farr SA, Poon HF, Dogrukol-AK D, Drake J, Banks WA, Euerman E, Butterfie DA, Morley JE. The antioxidants alpha-lipoic acid and N-acetylcysteine reverse memory impairment and brain oxidative stress in aged SAMP8 mice. J Neurochem 2003; 84: 1173-83.
Finazzi-Agro A, Menichelli A, Persiani M, Biancini G, Delprincipe D. Hydrogen peroxide release from human blood platelets. Biochim Biophys Acta 1982; 718: 21-5.
Flood JF, Harris FJ, Morley JE. Age-related changes in hippocampal drug facilitation of memory processing in SAMP8 mice. Neurobiol Aging 1996;17: 15-24.
Flood JF, Morley JE. Learning and memory in the SAMP8 mouse. Neurosci Biobehav Rev 1998; 22:1-20.
Forloni, F., Chiesa, R. & Smiroldo, S. (1993) Apoptosis mediated neurotoxicity induced by chronic application of beta amyloid fragment. Neuroreport 4: 523-6.
Geula, C., C. K. Wu, D. Saroff, A. Lorenzo, M. Yuan, and B. A. Yankner, Aging renders the brain vulnerable to amyloid beta-protein neurotoxicity: Nat Med, 1998; 4: 827-31.
Glenner, G. G., and C. W. Wong, Alzheimer''s disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein: Biochem Biophys Res Commun, 1984; 120: 885-90.
Gomes A, Vedasiromoni JR, Das M, Sharma RM, Ganguly DK. Anti- hyperglycemic effect of black tea (Camellia sinensis) in rat. J Ethnopharmacol 1995; 45: 223-236.
Goverde HJ, Dekker HS, Janssen HJ, Bastiaans BA, Rolland R, Zielhuis GA. Semen
quality and frequency of smoking and alcohol consumption--an explorative study. Int J Fertil Menopausal Stud 1995; 40: 135-138.
Halliwell B. and Gutteridge JMC. Free radicals in biology and medicine.
Clarendon Press 1989; 8: 484-487.
Halliwell, B. & Gutteridge, J. M. C. & Cross, C. E. Free radicals, antioxidants and human disease : where are we now ? J. Lab. Clin. Med. 1992; 119(6): 598-620.
Hao Z, Hang B, Wang Y: Hypoglycemic effect of fructus Ligustri Lucidi. Zhongguo Zhong Yao Za Zhi 1992; 17: 429-31.
Harman, D. Aging: a theory based on free radical and radiation chem. J. Gerontol. 1956; 11:298.
Hargreave TB. Genetics and male infertility. Curr Opin Obstet Gynecol 2000; 12: 207-219.
Hendin BN, Kolettis PN, Sharma RK, Thomas AJ Jr, Agarwal A. Varicocele is associated with elevated spermatozoal reactive oxygen species production and diminished seminal plasma antioxidant capacity. J Urol 1999; 161: 1831-1834.
Hooker, J. D. Chlorocodon whiteii- Curtis. Botanical Magazine. 1871; 5898 pp.
Hou WC, Hsu FL, Lee MH. Yam (Dioscorea batatas) tuber mucilage exhibited antioxidant activities in vitro. Planta Med 2002; 68: 1072-1076.
Hou WC, Lee MH, Chen HJ, Liang WL, Han CH, Liu YW, Lin YH. Antioxidant activities of dioscorin, the storage protein of yam (Dioscorea batatas Decne) tuber. J Agric Food Chem 2001; 49: 4956-4960.
Hsu HY. Infertility. Treatment with Chinese herbal preparations based on presenting
conformations. Int J Orient Med 1997; 22: 144-147.
Hsu PC, Liu MY, Hsu CC, Chen LY, Guo YLL. Effects of vitamin E and/or C on reactive oxygen species-related lead toxicity in the rat sperm. Toxicology 1998; 128: 169-179.
Hull MGR, Glazener CMA, Kelly NY. Population study of causes, treatment and outcome of infertility. Br Med J 1995; 291: 1693-1697.
Hu ML, Chen YK, Lin YF. The antioxidant and pro-oxidant activity of some B vitamins and vitamin- like component. Chem Biol Interact 1995; 97: 63-73.
Iwasaki A, Gagnon C. Formation of reactive oxygen species in species in spermatozoa of infertile patients. Fertil Steril 1992; 57: 409-416.
Jia J, Zhang X, Hu Y S, Wu Y. Evaluation of in vivo antioxidant activities of Ganoderma lucidum polysaccharides in STZ-diabetic rats. Food
Chemistry, 2009 ; 115(1):32-36.
Jones AR. Chemical interference with sperm metabolic pathways. J Reprod Fertil Suppl 1998; 53:227-234.
Kamtchouing P., Mbongue G.Y.F., Dimo T., Watcho P., Jatsa H.B. and Sokeng S.D.
Effects of Aframomum melegueta and Piper guineense on sexual behaviour of male rat. Behav Pharmacol 2002; 13: 243-247.
Karaca AG, Parker HM, Yeatman JB, McDaniel CD. The effects of heat stress and sperm quality classification on broiler breeder male fertility and semen ion concentrations. Br Poult Sci 2002; 43: 621-628.
Katzman, R. lzheimer’s disease. N. Engl. J. Med. 1986; 314: 964-971.
Kauto, H., Yokoi, I., Mori, A., Murakami, H. & Sawada, S. Neurochemical changes related to aging in the senescence-accelerated mouse brain and the effect of chronic administration of minodipine. Mech. Ageing Dev. 1995; 80: 1-9.
Kim, J. G. & Parthasarathy, S. Oxidation and the spermatozoa. Semin. Reprod. Edocrinol. 1998; 16: 235-239.
Kerharo, J. and J. G. Adam. La Pharmacop salaise traditionnelle:
Pants medicinales et toxiques. Paris: edition Vigot et Fres. 1974; 489 pp.
Kiho T, Ookubo K, Usui S, Ukai S, Hirano K. Structural features and hypoglycemic
activity of a polysaccharide (CS-F10) from the cultured mycelium of Cordyceps
sinensis. Biol Pharm Bull 22: 966-970, 1999.
Kins, S., A. Crameri, D. R. Evans, B. A. Hemmings, R. M. Nitsch, and J. Gotz, Reduced protein phosphatase 2A activity induces hyperphosphorylation and altered compartmentalization of tau in transgenic mice: J Biol Chem, 2001; 276;38193-200.
Klebanoff SJ. Phagocytic cells: products of oxygen metabolism. In: Gallin, J. I., Goldstein, I. M., Synderman, R. eds. Inflammation: Basic principles and clinical correlates. New York Raven 1988; 319-444.
Koorbanally, N. A., D. A. Mulholland and N. R. Crouch. Isolation of isovanillin from aromatic roots of the medicinal African liane, Mondia whitei . Journal of Herbs, Spices & Medicinal Plants. 2000; 7(3):37-44.
Kubo, I. and I. Kist-Hori. 2-Hydroxy-4-methoxybenzaldehyde: a potent tyrosinase from African medicinal plants. Planta Medica 1999;65;19-22.
Lee GH, Suriyaphan O, Cadwallader KR. Aroma components of cooked tail meat of American lobster (Homarus americanus). J Agric Food Chem. 2001;49: 4324-4332.
Li DJ, Li CJ, Zhu Y. Treatment of immunological infertility with Chinese medicinal herbs of aiyin jianghuo. Chung Kuo Chung His I Chieh Ho Tsa Chih 1995;15: 3-5.
Liu, J. & Mori, A. Age-associated changes in superoxide dismutase activity, thiobarbituric acid reactivity and reduced glutathione level in the rain and liver in senescence accelerated mice (SAM): a comparison with ddY mice. Mech Ageing Dev. 1993; 71(1-2): 23-30.
Lin SC, Chung TC, Lin CC, Ueng TH, Lin YH, Lin SY, Wang LY. Hepatoprotective effects of Arctium lappa on carbon tetrachloride- and acetaminophen-induced liver damage. Am J Chin Med 2000; 28: 163-73.
Lue T.F. Drug therapy: erectile dysfunction. N Engl J Med 2000; 342: 1802-1813.
Liu XD. Effect of Chinese medicinal herbs on sperm membrane of infertile male. Chung His I Chieh Ho Tsa Chih 1990; 10:515, 519-521.
Lorenzo, A., and B. A. Yankner, Beta-amyloid neurotoxicity requires fibril formation and is inhibited by congo red: Proc Natl Acad Sci 1994; 91: 12243-7.
Mann, T. Jones R. & Sherins, R. Oxygen damage, lipid peroxidation, and motility of spermatozoa. In “Testicular Development, Struction and Function” Steinberger, A. & Steinberger, E. Eds., Raven Press. New York. USA PP. 1980; 497-501.
Markesbery WR. Oxidative stress hypothesis in Alzheimer''s disease. Free
Radic Biol Med. 1997; 23:134-47.
Masaomi M, Yoshihiro K, Naoki Y, Akinobu N, Takao M, Yuji N, Toshio T.
Age-releated changes in learning and memory in the senescence-accelerated mice (SAM). Physiol Behav 1986; 38: 399-406. 
Morley JE. The SAMP8 mouse: a model of Alzheimer disease? Biogerontology 2002; 3: 57-60.
Masters, C. L., G. Multhaup, G. Simms, J. Pottgiesser, R. N. Martins, and K. Beyreuther, Neuronal origin of a cerebral amyloid: neurofibrillary tangles of Alzheimer''s disease contain the same protein as the amyloid of plaque cores and blood vessels: Embo 1985; 4: 2757-63.
Matsugo, S., Kitagawa, T., Minami, S., Esashi, Y., Oomura, Y., Tokumaru, S. & Kojo, S, Age-dependent changes in lipid peroxide levels in peripheral organs, but not in brain, in senescence-accelerated mice. Neurosci. Lett. 2000; 278: 105-108.
Matsugo, S., Yasui, F. & Sasaki, K. Analysis of the oxidative stress state in the brain and peripheral organs of senescence-accelerated mouse model. International Congress Series 2004; 1260: 251-253.
Mehlhorn, R. J. & Cole, G. The free radical theory of aging : a critical
review. Adv. Free Radical Biol. Med. 1985; 1: 165.
Minelli A, Moroni M, Castellini C, Lattaioli P, Mezzasoma I, Ronquist G. Rabbit
spermatozoa: a model system for studying ATP homeostasis and motility. J Androl 1999; 20: 259-266, 1999
Miyamoto, M. Characteristics of age-related behavioral changes in senescence-accelerated mouse SAMP 8 mice. In: Takeda T ed. The SAM model of senescence. Amsterdam: Excerpta Medica, Elsevier Science BV, 1994; 61-6.
Montorsi F., Salonia A., Deho F., Cestari A., Guazzoni G., Rigatti P. and Stief C. Pharmacological management of erectile dysfunction. Br J Urol 2003; 8: 211-216
Morley, J. E. The SAMP8 mouse: a model of Alzheimer disease? Biogerontology 2002; 3: 57-60.
Mortimer D, Pandya IJ, Sawers RS. Relationship between human sperm motility
characteristics and sperm penetration into human cervical mucus in vitro. J Reprod Fertil 1986; 78: 93-102.
Mosher, W. D., & Pratt, W. F. Fecundity and Infertility in the United States: Incidence and Trends. Fertil Steril, 1991; 56, 192-193.
Msonthi, J. D. A novel phenolic glycoside from Mondia whitei Skeels. Bull.Ch em. Soci. Ethiop 1991; 5: 107-110.
Mukonyi, K. W. and I. O. Ndiege. 2-Hydroxy-4-methoxybenz-aldehyde: aromatic taste modifying compound from Mondia whitei Skeels. Bull.Ch em. Soci. Ethiop 2001; 15:137-141.
Myhre, A. & Tysnes, O. B. Etiology and genetics of Alzheimer’s disease. Tidsskrift for Den Norske Laegeforening. 2002; 122: 50-53.
Nakazawa, Y. Functional and healthy properties of Du-zhoung tea and their utilization. Food industry (in Japamese) 1997; 40:6-15.
Namik KD, Mahmood M, Sergio O. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Reprod Biol 2000; 74: 1200-1207.
Newton, R. K., Ducore, J. M. & Sohal, R. S. Effect of age on endogenouse DNA single-strand breakage, strand break induction and repair in the adult housefly. Muatation Res. 1989; 219:113.
Noumi E., Amvam Zollo P.H. and Lontsi D. Aphrodisiac plants used in Cameroon. Fitoterapia 1998; 2: 125-134.
Noumi, E., Z. P. H. Amvam and D. Lontsi. Aphrodisiac plants used in Cameroon. Fitoterapia 1998;69(2): 125-134.
Ohnishi Y, Takagi S, Miura T, Usami M, Kako M, Ishihara E, Yano H, Tanigawa K, Seino Y. Effect of ginseng radix on GLUT2 protein content in mouse liver in normal and epinephrine-induced hyperglycemic mice. Biol Pharm Bull 1996; 19: 1238-1240.
Okuma Y, Nomura Y. Senescence-accelerated mouse (SAM) as an animal model of senile dementia: pharmacological, neurochemical and molecular biological approach. Jpn J Pharmacol 1998; 74: 399-404.
Oryem-Origa, H . E. K. Z. Kakudidi, A. B. Katende and Z.R. Bukenya. Preliminary ethno botanical studies of the Rwenzori Mountain forest area in Bundibugyo District, Uganda. Bothalia. 1995; 25(1): 111-119.
Padron OF, Brackett NL, Sharma RK, Lynne CM, Thomas AJ Jr, Agarwal A. seminalreactive oxygen species and sperm motility and morphology in men with spinal cord injury. Fertil Steril 1997; 67: 1115-1120.
Park SY, Bok SH, Jeon SM, Park YB, Lee SJ, Jeong TS, Choi MS. Effect of tutin and tannic acid supplements on cholesterol metabolism in rats. Nutr Res 2002; 22: 283-95.
Parazzini F, Marchini M, Tozzi L, Mezzopane R, Fedele L. Risk factors for unexplained dyspermia in infertile men: a case-control study. Arch Androl 1993; 31: 105-113.
Patnam, R., S. S. Kadali, K. H. Koumaglo, R. Roy. A chlorinated coumarinolignan from Africa medicinal plant, Mondia whitei, 2005; 66: 683-686.
Pike, C. J., D. Burdick, A. J. Walencewicz, C. G. Glabe, and C. W. Cotman, Neurodegeneration induced by beta-amyloid peptides in vitro: the role of peptide assembly state: J Neurosci, 1993; 13; 1676-8.
Pike, C. J., Walencewicz, A. J., Glabe, C. G. & Cotman, C. W. In vitro aging of beta-amyloid protein cause peptide aggregation and neurotoxicity. Brain Res. 1991; 563: 311-314.
Poon HF, Joshi G, Sultana R, Farr SA, Banks WA, Morley JE, Calabrese V, Butterfield DA. Antisense directed at the Abeta region of APP decreases brain oxidative markers in aged senescence accelerated mice. Brain Res. 2004;1018:86-96.
Rebrin, I. & Sohal, R. S. Comparison of thiol redox state of mitochondria and homogenates of various tissues between two strains of mice with different longevities. Exp. Gerontol. 2004; 39(10): 1513-9.
Reiss, U. & Gershon, D. Rat-liver superoxide dismutase. Purification and age-related modifications. Eur. J. Biochem. 1976; 63(2): 617-623.
Saleh RA, Agarwal A, Sharma RK, Nelson DR, Thomas AJ Jr. Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study. Fertil Steril 2002; 78: 491-499.
Sandy MS., Moldeus P., Ross D. and Smith MT. Role of redox cycling and
lipid peroxidation in bipyridyl herbicide cytotoxicity.Studies with a
comptomised isolated hepatocyte model system. Bio Pharm 1986; 35: 3095-3101.
Selkoe DJ. Amyloid protein and Alzheimer’s disease. Sci Am 1991; 265: 68-71.
Schrader SM, Kanitz MH. In, Gold E, Schenker M, Lesley B. State of the art reviews in occupational medicine, reproductive hazards. PA, Hanley & Belfus, Inc., 1994; 405-414.
Shabsigh R. and Anastasiadis A.G. (2003). Erectile dysfunction. Annu Rev Med 2003; 54: 153-168
Shan BE, Yoshida Y, Kuroda E, Yamashita U. Immunomodulating activity of seaweed extract on human lymphocytes in vitro. Int J Immunopharmacol 1999; 21: 59-70.
Sies H, Stahl W. Vitamin E and C, β-carotene, and other carotenoids as antioxidante. Am J Clin Nutr 1995; 62: 1315S–21S.
Sigman M, Lipshurtz L, Howards S. In., Infertility in the male. Lipshultz LA and
arow JP. Effects of varicocele on male fertility. Hum Reprod Update 2001; 7: 59-64.
Sikka SC, Rajasekaran M, Hellstrom WJ. Role of oxidative stress and antioxidants in male infertility. J Androl 1995; 16: 464-481.
Sinawat S. The environmental impact on male fertility. J Med Assoc Thai 2000; 83: 880-885.
Sohal, R. S. & Allen, R. G. Oxidative stress as a causal factor in differentiation and aging: a unifying hypothesis. Exp. Gerontol. 1990; 25: 499.
Takeda T, Hosokawa M, Higuchi K. Senescence-accelerated mouse
(SAM): a novel murine model of accelerated senescence. J Am Geriatr Soc 1991; 39:911-9.
Takeda T, Hosokawa M, Takeshita S, Irino M, Higuchi K, Matsushita T, Tomita Y, Yasuhira K, Hamamoto H, Shimizu K, Ishii M, Yamamuro T. A new murine model of accelerated senescence. Mech Ageing Dev 1981; 17: 183-94.
Talesa, V. N., 2001, Acetylcholinesterase in Alzheimer''s disease: Mech Ageing Dev, 2001;122; 1961-9.
Tanzi RE, McClatchey AI, Lamperti ED, Lydia VK, Gusella JF, Neve RL. Proteinase inhibitor domain encoded by an amyloid protein precursor mRNA associated with Alzheimer’s disease. Nature 1988; 331: 528-30.
Telisman S, Cvitkovic P, Jurasovic J, Pizent A, Gavella M, Rocic B. Semen quality and reproductive endocrine function in relation to biomarkers of lead, cadmium, zinc, and copper in men. Environ Health Perspect 2000; 108: 45-53.
Thiele, J. J., Friesleben, H. J., Fuchs, J. & Ochsendorf, F. R. Ascorbic acid and urate in human seminal plasma: determination and interrelationships with chemiluminescence in washed semen. Hum. Reprod. 1995; 10: 110-115.
Watcho, P.; Fotsing, D.; Zelefack, F.; Nguelefack, T.; P Kamtchouing; Tsamo, E.; Kamanyi, A., Effects of Mondia whitei extracts on the contractile responses of isolated rat vas deferens to potassium chloride and adrenaline. Indian Journal of Pharmacology 2006; 38:33-37.
Watcho P, Kamtchouing P, Sokeng SD, Moundipa PF, Tantchou J, Essame JL, Koueta N. Androgenic effect of Mondia whitei roots in male rats. Asian J Androl. 2004;6(3):269-72.
Wolff H. The biologic significance of white blood cells in semen. Fertil Steril 1995;63:1143-1157.
Wong WY, Thomas CM, Merkus HM, Zielhuis GA, Doesburg WH, Steegers-Theunissen RP. Cigarette smoking and the risk of male factor subfertility: minor association between cotinine in seminal plasma and semen morphology. Fertil Steril 2000; 74: 930-935.
Wyrobek, A. & Bruce, W. R. The induction of sperm-shape in mice and humans. In “Chemical mutagens principies and methods for their detection” Hollanender, C. A. Ed., Raven Press, New York. 1978;257-280 Pp.
Yagi, K., Yoshino, K. & Komura, S. Lipid peroxide level in the senescence- accelerated mouse. J. Clin, Biochem. Nutr. 1988; 5: 21-27.
Yamahara J, Mibu H, Sawada T, Fujimura H, Takino S, Yoshikawa M, Kitagawa I.
Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001; 54: 176-86.
Yen, G.C. and Hsieh, C.L. 2000. Reactive oxygen species scavenging activity of Du-zhong (Eucommia ulmoides Oliv.) and its active compounds. J. Agric. Food. Chem. 48:3431-3436.
Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001;54: 176-86.
Yang, J., Kato, K., Noguchi. K., Dairaku, N., Koke, T., Iijima,. K.,Sekin, H., Ohara S., Sasano,H., Tece. and Shimacc, T. Tochu (Eucommia ulmoides) leaf extract prevents ammonia and vitamin C deficiency induced gastric mucosal injury. Life Sci. 2003; 73:3245-3215.
Zahalsky MP, Zoltan E, Medley N, Nagler HM. Morphology and the sperm penetration assay. Fertil Steril 2003; 79: 39-41.
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