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研究生:廖弘玉
研究生(外文):Henry Liao
論文名稱:四環素類抗生素在畜產廢水中生物降解及菌群分析
指導教授:張碧芬張碧芬引用關係
學位類別:碩士
校院名稱:東吳大學
系所名稱:微生物學系
學門:生命科學學門
學類:微生物學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:129
中文關鍵詞:四環素類抗生素生物降解
外文關鍵詞:tetracycline
相關次數:
  • 被引用被引用:1
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摘要

台灣畜產業常以集約的方式經營,以便提高產品的產量,使得產品可作為人類生活所需優良蛋白質的來源。然而過於集約的方式,常造成養殖生物集體感染疾病而大量死亡,導致產品產量驟減與經濟上嚴重的損失。為了降低生物的疾病暴發,業者經常會使用抗生素來預防或治療疾病因此在環境中造成殘留,殘留的四環素類抗生素可能會危及環境中的水生生物生長,或是造成細菌抗藥性的發生,間接的增加人類健康的風險。因此需要探討四環素類抗生素在養殖環境中的殘留分佈與降解半衰期,以便更進一步來解決抗生素殘留的問題。
Tetracycline antibiotics (TCs) is one of the most extensively and
legally used antibiotics in aquaculture of Taiwan. However, since the low
bioavailability and strong solid absorbtion of TCs, many TCs residual cases
reported in the environment. The residual TCs may cause adverse impact
on aquatic organisms, raise the resistance of microorganisms and increase
indirect risk of human health. Therefore, it is necessary to be fully studied
in the fates of TCs in aquaculture environments.
The aims of this study were to understand the effects of natural light
and microbial activity on degradation of TCs that exist in the aquaculture
pond sediments. In aquaculture, limes and oxidants are used to adjust pH or
to disinfect bacteria in aquaculture ponds. Therefore, the effects of limes
and oxidants on the degradation of TCs in pond sediment slurries were also
elucidated. The TCs included oxytetracycline (OTC), tetracycline (TC),
chlortetracycline (CTC) and doxycycline (DC). The effect of light
experiment was conducted with both of the natural light and dark
treatments. The effects of microbial activity were conducted with both of
non-sterile and sterile. The applied limes included calcium carbonate
(CaCO3), calcium oxide (CaO), and calcium hydroxide (Ca(OH)2).
Furthermore, The six kinds of oxidants, namely calcium hypochlorite
(Ca(OCl)2), chlorine dioxide (ClO2), hydrogen peroxide (H2O2), sodium
nitrate(NaNO3), potassium permanganate (KMnO4) and sodium
IV
hypochlorite (NaOCl) were used. Moreover, the interactive effects between
natural light and the treatments of limes and oxidants on degradation of
TCs were also studied.
1.摘要..........I
2.前言..........1
3.材料方法.....14
4.結果.........42
5.討論.........66
6.表...........84
7.圖...........98
Bourbonnais, R., Paice, M.G., 1990. Oxidation of non-phenolic substrates. An expanded role for laccase in lignin biodegradation. FEBS Letters 267, 99-102.
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