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研究生:徐銘謙
研究生(外文):Ming-Chien, Hsu
論文名稱:植物荷爾蒙和蔗糖濃度在葡萄細胞培養下對原花青素、鞣花酸及白藜蘆醇生合成之影響
論文名稱(外文):The effects of growth regulators and sucrose on the production of proanthocyanidin, ellagic acid and resveratrol in Vitis vinifera cell cultures
指導教授:王建國王建國引用關係
指導教授(外文):Chien Kuo, Wang
學位類別:碩士
校院名稱:亞洲大學
系所名稱:生物科技學系碩士班
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:85
中文關鍵詞:葡萄細胞培養二次代謝物
外文關鍵詞:grapecell culturessecondary metabolites
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由於葡萄所含的多酚類化合物包括原花青素(proanthocyanidin)、鞣花酸(ellagic acid)及白藜蘆醇(resveratrol)常被廣泛的應用於食品、醫療、化妝品、醫療工程上,例如:抗人體低密度酯蛋白的氧化、增強免疫系統、抗癌、預防高血壓、抗氧化。故以葡萄(Vitis vinifera L. Gamay Freaux) 癒傷組織細胞(FC-01)為培養於54 種條件下,萃取出原花青素、鞣花酸及白藜蘆醇這三種物質並比較其不同調間培養下其含量的差異,另將萃取物做酪胺酸酶活性之抑制試驗,以對應原花青素、鞣花酸及白藜蘆醇之生合成量是否等同於酪胺酸酶活性之抑制效能。結果顯示出來源自澳洲Flinders University 的FC-01 紅色細胞株,在懸浮培養條件為0.3 mg/L NAA + 0.3 mg/L kinetin + 50 g/L sucrose 培養下原花青素生合成量最高為7.541 mg/L,而在懸培養條件為0.1 mg/L NAA + 0.1 mg/L kinetin + 30 g/L sucrose 培養下能同時誘導原花青素、鞣花酸及白藜蘆醇的生合成,另在懸培養條件為0.1 mg/L 2,4-D +
0.2 mg/L kinetin + 30 g/L sucrose 培養下,測得白藜蘆醇含量為5.212 mg/L。而在酪胺酸酶活性之抑制試驗結果顯示原花青素、鞣花酸及白藜蘆醇之生合成量與酪胺酸酶活性之抑制效能並沒有顯著的相關。此實驗也顯示了以不同的環境因子如:蔗糖濃度的調控、植物荷爾蒙濃度的調控,不同的生長條件能誘導增加原花青素、鞣花酸及白藜蘆醇
在FC-01 葡萄(Vitis vinifera L. Gamay Freaux) 癒傷組織細胞中的產量,且由於尚未有文獻指出藉由改變培養條件能使葡萄癒傷組織細胞能生合成鞣花酸,故此實驗結果大大的提高了葡萄癒傷組織細胞之利用價值。
The antioxidants in grapes such as proanthocyanidins, ellagic acid and resveratrol are usually applied in food, medical and cosmetic products. Previous studies have shown that the changes of environmental factors such as UV light treatment, sucrose concentration and plant growth regulators, could increase the contents of these antioxidants in Vitis vinifera L. Gamay Freaux cultures. Therefore, we use 54 kinds of suspension culture conditions which use plant growth regulators for the FC-01 grape callus (Vitis vinifera L. Gamay Freaux). The extracts(proanthocyanidins, ellagic acid and resveratrol) from FC-01 were tested with inhibition of tyrosinase activity. The results have shown FC-01 in suspension culture conditions for the 0.3 mg/L NAA + 0.3 mg/L kinetin + 50g/L sucrose cultured in the amount of proanthocyanidin biosynthesis up to 7.541 mg/L. By applying the suspension culture condition (0.1 mg/L NAA + 0.1 mg/L kinetin + 30 g/L sucrose), we were able to induce proanthocyanidins, ellagic acid and resveratrol biosynthesis. Additionally, by applying another suspension culture condition (0.1 mg/L 2, 4-D + 0.2 mg/L kinetin + 30 g/L sucrose), we were also able to culture the amount of resveratrol up to 5.212 mg/L. The tyrosinase activity inhibition test showed that the amount of biosynthesis of proanthocyanidins, ellagic acid and resveratrol was not significantly related. Furthermore, this experiment has shown that different plant growth factors such as the regulation of sucrose concentration and plant hormone concentration can induce an increase in the amount of the biosynthesis of proanthocyanidins, ellagic acid and resveratrol in FC-01, and subsequently improve the value of the use of FC-01.
壹、摘要
貳、英文摘要
參、前言
肆、文獻回顧
一、植物二次代謝物
(一)原花青素(proanthocyanidins)
(二)鞣花酸(ellagic acid:EA)
(三)白藜蘆醇 (resveratrol)
二、植物生長激素
三、黑色素簡介
四、高效能液相層析儀(high-performance liquid chromatography, HPLC)
肆、研究目的
伍、材料與方法
一、藥品與配製:
二、HPLC儀器與條件:
三、植物細胞株(cell line)和培養的條件
四、細胞懸浮培養與取樣
五、實驗設計方法
六、萃取與測定
七、酪胺酸酶活性之抑制試驗
八、實驗數據統計分析
陸、結果與討論
一、原花青素、鞣花酸和白藜蘆醇標準品在HPLC分析之滯留時間
二、生長條件對FC-01細胞株生合成原花青素、鞣花酸和白藜蘆醇的影響
三、FC-01細胞株之萃取物對酪胺酸酶活性之抑制效能
柒、結論
捌、參考文獻
1.劉承煌;皮膚病理學。中國醫藥科技出版社(1990)。
2.邱念華;儀器分析實驗。新文京開發(2003)。
3.陳世昌;植物組織培養。重慶大學出版社(2008)
4.Aumont, V., Larronde, F., Richard, T., Budzinski, H., Decendit, A., Deffieux, G., Krisa, S. and Mérillon, J. M., “Production of highly 13C-labeled polyphenols in Vitis vinifera cell bioreactor cultures”, Journal of Biotechnology 109 (2004) 287-294.
5.Bogs, J., Mark O. Downey, M. O., Harvey, J. S., Ashton, A. R., Tanner, G. J., Robinson, S. P. “Proanthocyanidin Synthesis and Expression of Genes Encoding Leucoanthocyanidin Reductase and Anthocyanidin Reductase in Developing Grape Berries and Grapevine Leaves”, Plant Physiol 139 (2005) 652-663.
6.Breuil A. C., Adrian M., Pirio, N., Meunier, P., Bessis, R., Jeandet, P., “Metabolism of Stilbene Phytoalexins by Botrytis cinerea : 1. Characterization of a Resveratrol Dehydrodimer”, Tetrahedron Letters 39(1998)537-540.
7.Castrejon, A. D. R., Eichholz, I., Rohn, S., Kroh, L. W., Huyskens-Keil, S., “Phenolic profile and antioxidant activity of highbush blueberry (Vaccinium corymbosum L.) during fruit maturation and ripening”, Food Chemistry 109(2008)564-572.
8.Comis, R. L. and Friedland, D. M., “New chemotherapy agents in the treatment of advanced non-small cell lung cancer: An update including data from the seventh world conference on lung cancer”, Lung Cancer 12 Suppl. 2 (1995) S63-S99.
9.Cormier, F., Crevier, H. A. and Do, C. B., “Effects of sucrose concentration on the accumulation of anthocyanins in grape (Vitis vinifera) cell suspension. Can J Bot(1990)68:1822-1825.
10.Decendit, A., Waffo-Teguo, P., Richard, T., Krisa, S., Vercauteren, J., Monti, J. P., Deffieux, G. and Merillon, J. M., “Galloylated catechins and stilbene diglucosides in Vitis vinifera cell suspension cultures”, Phytochemistry 60 (2002) 795-798
11.Do, C. B. and Cormier, F., “Accumulation of anthocyanins enhanced by a high osmotic potential in grape (Vitis vinifera L.) cell suspensions”, Plant Cell Rep 9(1990)143-146.
12.Donnez, D., Kim, K. H., Antoine, S., Conreux, A., Luca, V. D., Jeandet, P., Clementa, C. and Courot, E., “Bioproduction of resveratrol and viniferins by an elicited grapevine cell culture in a 2 L stirred bioreactor”, Process Biochemistry 46 (2011) 1056-1062.
13.Ernfors, P., “Cellular origin and developmental mechanisms during the formation of skin melanocytes”, Experimental Cell Research 316 (2010)1397-1407.
14.Ferri, M., Righetti, L. and Tassoni, A., “Increasing sucrose concentrations promote phenylpropanoid biosynthesis in grapevine cell cultures”, Journal of Plant Physiology 168 (2011) 189-95.
15.Funk C. and Brodelius, P. E., “Influence of growth regulators and an elicitor on phenylpropanoid metabolism in suspension cultures of Vanilla planifolia”, Phytochemistry 29 (1990) 845-848.
16.Funk C. and Brodelius, P. E., “Phenylpropanoid Metabolism in Suspension Cultures of Vanilla planifolia Andr.”, Plant Physiol. 99(1992)256-262
17.Gurbuz, O., Gocmen, D., Dagdelen, F., Gursoy, M., Aydin, S., Sahin, I., Buyukuysal, L., Usta, M., “Determination of flavan-3-ols and trans-resveratrol in grapes and wine using HPLC with fluorescence detection”, Food Chemistry 100(2007)518-525.
18.Haseeb, N., John, S., Gauri, S. M., Yukio, K., “Extraction of polyphenols from grape seeds and concentration by ultrafiltration”, Separation and Purification Techno l8(2006)176-181.
19.Hayes, J. E., Allen P., Brunton N., O’Grady b, M. N., Kerry, J. P., “Phenolic composition and in vitro antioxidant capacity of four commercialphytochemical products: Olive leaf extract (Olea europaea L.), lutein, sesamol and ellagic acid”, Food Chemistry 126(2011) 948-955.
20.Hirasuna, T. J., Shuler, M. L., Lackney, V. K. and Spanswick, R.M., “Enhanced anthocyanin production in grape cell cultures” Plant Sci 78(1991)107–120.
21.Hopkins, W. G. and Huner N. P. A. “Introduction to Plant physiology”. (2004)
22.Janis, A and Desmond J. T. Melanosomal pH Controls Rate of Melanogenesis, Eumelanin/Phaeomelanin Ratio and Melanosome Maturation in Melanocytes and Melanoma Cells. Experimental Cell Research 268(2001)26–35.
23.Jo, J-Y, MEJIA , E. G. D., LILA, M. A. “Effects of Grape Cell Culture Extracts on Human Topoisomerase II Catalytic Activity and Characterization of Active Fractions” , J. Agric. Food Chem. 53 (2005)2489-2498.
24.Kramer, M., Bongaerts, J., Bovenberg, R., Kremer, S., Muller, U.,
Orf, S., Wubbolts, M. and Raeven, L., “Metabolic engineering for microbial production of shikimic acid”, Metabolic Engineering 5 (2003) 277–283.
25.Krasnow, M. and Murphy, T. M., “Polyphenol Glucosylating Activity in Cell Suspensions of Grape(Vitis vinifera)”, J. Agric. Food Chem. 52(2004)3467-3472.
26.Kuo, H. S., “Production of resveratrol by cell culture of Vitis thunbergii Sieb. et Zucc.”, Science Department of Bioengineering
Tatung University (2006)
27.Larronde, F., Krisa, S., Decendit, A., Cheze, C., Deffieux, G. and Merillion, J. M., “Regulation of polyphenol production in Vitis vinifera cell suspension cultures by sugars”, Plant Cell Rep 17(1998)946-950.
28.Lee, J. H. and Talcott, S. T., “Ellagic Acid and Ellagitannins Affect on Sedimentation in Muscadine Juice and Wine”, J. Agric. Food Chem.50(2002)3971-3976.
29.Lee, J. H., Johnson, J. V. and Talcott, S. T., “Identification of Ellagic Acid Conjugates and Other Polyphenolics in Muscadine Grapes by HPLC-ESI-MS”, J. Agric. Food Chem. 53(2005)6003-6010.
30.Lei, Z., “Monomeric Ellagitannins in Oaks and Sweetgum”, A dissertation submitted to faculty of Virginia Polytechnic Institute and State University in partial fulfillment of the requirements for the Degree of Doctor of Philosophy in Wood Science and Forest Products. (2002)
31.Luximon-Ramma, A., Bahorun, T., Crozier, A., Zbarsky, V., Datla, K.P., Dexter, D.T., Aruoma, O. I., “Characterization of the antioxidant functions of flavonoids and proanthocyanidins in Mauritian black teas”, Food Res. Intern 38(2005)357–367.
32.Manna, S. K., Mukhopadhyay, A. and Aggarwal, B. B., “Resveratrol Suppresses TNF-Induced Activation of Nuclear Transcription Factors NF- kB, Activator Protein-1, and Apoptosis: Potential Role of Reactive Oxygen Intermediates and Lipid Peroxidation”, The Journal of Immunology 64(2000)6509-6519.

33.Mario J. Simirgiotis, M. J. and Schmeda-Hirschmann, G., “Determination of phenolic composition and antioxidant activity in fruits, rhizomes and leaves of the white strawberry (Fragaria chiloensis spp. Chiloensis form chiloensis) using HPLC-DAD-ESI-MS and free radical quenching techniques”, Journal of Food Composition and Analysis 23 (2010) 545-553
34.Matkowski, A., “Plant in vitro culture for the production of antioxidants -A review”, Biotechnology Advances 26(2008) 548-560.
35.Mertens-Talcott, S. U., Percival, S. S., “Ellagic acid and quercetin interact synergistically with resveratrol in the induction of apoptosis and cause transient cell cycle arrest in human leukemia cells”, Cancer Letters 218(2005)141-151.
36.Moreno, D. A., Ilic, N., Poulev, A., Brasaemle, D. L., Fried, S. K. and Raskin, I., “Inhibitory Effects of Grape Seed Extract on Lipases”, Nutrition 19(2003)876-879.
37.Narayanana, B. A.,Geoffroya, O., Willinghama, M. C., Re, G. G., Nixona, D. W. “p53/p21(WAF1/CIP1) expression and its possible role in G1 arrest and apoptosis in ellagic acid treated cancer cells”, Cancer Letters 136(1999)215-221.
38.Nandakumar, V., Singh, V. T., Katiyar, S. K., “Multi-targeted prevention and therapy of cancer by proanthocyanidins”, Cancer Letters 26 (2008)378-387.
39.Nawaz, H., Shi, J., Mittal G. S., Kakuda, Y., “Extraction of polyphenols from grape seedsand concentration by ultrafiltration”, Separation and Purification Technology 48(2006)176-181.
40.Ng, L. T., Ko, H. H., Lu, T. M., “Potential antioxidants and tyrosinase inhibitors from synthetic polyphenolic deoxybenzoins”, Bioorganic & Medicinal Chemistry 17 (2009) 4360-4366.
41.Nicolai, M., Gonçalves, G., Natalio, F., Humanes, M., “Biocatalytic formation of synthetic melanin: The role of vanadium haloperoxidases, L-DOPA and iodide“, Journal of Inorganic Biochemistry 105(2011)887-893.
42.Pasqua, G., Silvestrini, A., Monacelli a, B., Mulinacci, N., Menendez, P., Botta, B. , “Triterpenoids and ellagic acid derivatives from in vitro cultures of Camptotheca acuminata Decaisne”, Plant Physiology and Biochemistry 44(2006)220-225.
43.Pastrana-Bonilla, E., Akoh, C. C.,Sellappan, S. and Krewer, G., “Phenolic Content and Antioxidant Capacity of Muscadine Grapes”, J. Agric. Food Chem. 51(2003) 5497-5503.
44.Prasad, K. N., Yang, B., Yang, S., Chen, Y., Zhao, M., Ashraf, M., Jiang, Y., “Identification of phenolic compounds and appraisal of antioxidant and antityrosinase activities from litchi (Litchi sinensis Sonn.) seeds”, Food Chem 116 (2009)1-7.
45.Rao, S. R. and Ravishankar, G. A., “Plant cell cultures:
Chemical factories of secondary metabolites”, Biotechnology Advances 20 (2002) 101-153.
46.Revilla, E. and Ryan, J. M., “Analysis of several phenolic compounds with potential antioxidant properties in grape extracts and wines by high-performance liquid chromatography–photodiode array detection without sample preparation”, Journal of Chromatography A 881 (2000) 461-469.
47.Rozanowska, M., Sarna, T., Land, E. J., Truscott, T. G. “Free Radical Scavenging properties of Melanin Interaction of EU-and Pheo-Melanin Models with Reducing and Oxidising Radical s”, Free Radical Biology & Medicine 26(1999)518-525.
48.Saigne-Soulard, C., Richard, T., Merillion, J. M., Monti, J. P., “13C NMR analysis of polyphenol biosynthesis in grape cells: impact of various inducing factor”, Anal Chim Acta 563(2006)137-144.
49.Saiko, P., Szakmary, A., Jaeger, W., Szekeres, T., “Resveratrol and its analogs: Defense against cancer, coronary disease and neurodegenerative maladies or just a fad?”, Mutation Research 658 (2008) 68-94.
50.Sairam, G., Franklin, R., Hassel, B., Smith, K., Meeker, N., Kashikar, M., Parani, D. Al., Abed, S., Ismail, K., Berry, S. L. G. “A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus” Plant Cell, Tissue and Organ Culture 75(2003) 79-85.
51.Sandhu, A. K. and Gu, L., “Antioxidant Capacity, Phenolic Content, and Profiling ofPhenolic Compounds in the Seeds, Skin, and Pulp of Vitis rotundifolia (Muscadine Grapes) As Determined by HPLC-DAD-ESI-MSn”, J. Agric. Food Chem. 58(2010) 4681-4692.
52.Teguo, P. W., Decendit, A., Vercauteren, J., Deff ieux, G. and Merillon, J. M., “trans-Resveratrol-3-O-glucoside (Piceid) in cell suspension cultures of Vitis Vinifera”, Phytochemistry 42(1996)1591-1593.
53.Vaquero, M. J. R., Serravalle, L. R. T., Nadra , M. C. M. de and Saad, A. M. S. de, “Antioxidant capacity and antibacterial activity of phenolic compounds from argentinean herbs infusions”, Food Control 21 (2010) 779-785.
54.Victor, S. S. and Richard J. C. “trans-Resveratrol Content in Commercial Peanuts and Peanut Products”, J. Agric. Food Chem. 47(1999)1435-1439.

55.Vitrac, X., Krisa, S., Decendit, A., Vercauteren, J., Nuhrich, A., Monti, J. P., Deffieux, G. and Merillon, J. M., “Carbon-14 biolabelling of wine polyphenols in Vitis inifera cell suspension cultures”, Journal of Biotechnology 95 (2002) 49-56.
56.Wang, H., Arakawa O., and Y. Motomura:Influence of maturity and bagging on the relationship between anthocyanin accumulation and phenylalanine ammonialyase (PAL) activity in ‘Jonathan’ apples. Postharvest Biol. Tech. 19(2000)123-128
57.Wang. W., Tang, K., Yang, H. R., Wen, P. F., Zhang, P., Wang, H. L., Huang, W. D., “Distribution of resveratrol and stilbene synthase in young grape plants (Vitis vinifera L. cv. Cabernet Sauvignon) and the effect of UV-C on its accumulation”, Plant Physiology and Biochemistry 48(2010)142-152.
58.Yoshimura, M., Watanabe, Y., Kasai, K., Yamakoshi, J., Koga, T., “Inhibitory Effect of an Ellagic Acid-Rich Pomegranate Extract on Tyrosinase Activity and Ultraviolet-Induced Pigmentation”, Biosci. Biotechnol. Biochem. 69(2005) 2368-2373,
59.Zhang, W., Curtin, C., Kikuchi, M., Franco, C., “Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis inifera suspension cultures”, Plant Science 162 (2002)459-468.
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