由於葡萄所含的多酚類化合物包括原花青素(proanthocyanidin)、鞣花酸(ellagic acid)及白藜蘆醇(resveratrol)常被廣泛的應用於食品、醫療、化妝品、醫療工程上，例如：抗人體低密度酯蛋白的氧化、增強免疫系統、抗癌、預防高血壓、抗氧化。故以葡萄(Vitis vinifera L. Gamay Freaux) 癒傷組織細胞(FC-01)為培養於54 種條件下，萃取出原花青素、鞣花酸及白藜蘆醇這三種物質並比較其不同調間培養下其含量的差異，另將萃取物做酪胺酸酶活性之抑制試驗，以對應原花青素、鞣花酸及白藜蘆醇之生合成量是否等同於酪胺酸酶活性之抑制效能。結果顯示出來源自澳洲Flinders University 的FC-01 紅色細胞株，在懸浮培養條件為0.3 mg/L NAA + 0.3 mg/L kinetin + 50 g/L sucrose 培養下原花青素生合成量最高為7.541 mg/L，而在懸培養條件為0.1 mg/L NAA + 0.1 mg/L kinetin + 30 g/L sucrose 培養下能同時誘導原花青素、鞣花酸及白藜蘆醇的生合成，另在懸培養條件為0.1 mg/L 2,4-D +
0.2 mg/L kinetin + 30 g/L sucrose 培養下，測得白藜蘆醇含量為5.212 mg/L。而在酪胺酸酶活性之抑制試驗結果顯示原花青素、鞣花酸及白藜蘆醇之生合成量與酪胺酸酶活性之抑制效能並沒有顯著的相關。此實驗也顯示了以不同的環境因子如：蔗糖濃度的調控、植物荷爾蒙濃度的調控，不同的生長條件能誘導增加原花青素、鞣花酸及白藜蘆醇
在FC-01 葡萄(Vitis vinifera L. Gamay Freaux) 癒傷組織細胞中的產量，且由於尚未有文獻指出藉由改變培養條件能使葡萄癒傷組織細胞能生合成鞣花酸，故此實驗結果大大的提高了葡萄癒傷組織細胞之利用價值。

The antioxidants in grapes such as proanthocyanidins, ellagic acid and resveratrol are usually applied in food, medical and cosmetic products. Previous studies have shown that the changes of environmental factors such as UV light treatment, sucrose concentration and plant growth regulators, could increase the contents of these antioxidants in Vitis vinifera L. Gamay Freaux cultures. Therefore, we use 54 kinds of suspension culture conditions which use plant growth regulators for the FC-01 grape callus (Vitis vinifera L. Gamay Freaux). The extracts(proanthocyanidins, ellagic acid and resveratrol) from FC-01 were tested with inhibition of tyrosinase activity. The results have shown FC-01 in suspension culture conditions for the 0.3 mg/L NAA + 0.3 mg/L kinetin + 50g/L sucrose cultured in the amount of proanthocyanidin biosynthesis up to 7.541 mg/L. By applying the suspension culture condition (0.1 mg/L NAA + 0.1 mg/L kinetin + 30 g/L sucrose), we were able to induce proanthocyanidins, ellagic acid and resveratrol biosynthesis. Additionally, by applying another suspension culture condition (0.1 mg/L 2, 4-D + 0.2 mg/L kinetin + 30 g/L sucrose), we were also able to culture the amount of resveratrol up to 5.212 mg/L. The tyrosinase activity inhibition test showed that the amount of biosynthesis of proanthocyanidins, ellagic acid and resveratrol was not significantly related. Furthermore, this experiment has shown that different plant growth factors such as the regulation of sucrose concentration and plant hormone concentration can induce an increase in the amount of the biosynthesis of proanthocyanidins, ellagic acid and resveratrol in FC-01, and subsequently improve the value of the use of FC-01.

壹、摘要
貳、英文摘要
參、前言
肆、文獻回顧
一、植物二次代謝物
(一)原花青素(proanthocyanidins)
(二)鞣花酸(ellagic acid：EA)
(三)白藜蘆醇 (resveratrol)
二、植物生長激素
三、黑色素簡介
四、高效能液相層析儀（high-performance liquid chromatography, HPLC）
肆、研究目的
伍、材料與方法
一、藥品與配製:
二、HPLC儀器與條件:
三、植物細胞株(cell line)和培養的條件
四、細胞懸浮培養與取樣
五、實驗設計方法
六、萃取與測定
七、酪胺酸酶活性之抑制試驗
八、實驗數據統計分析
陸、結果與討論
一、原花青素、鞣花酸和白藜蘆醇標準品在HPLC分析之滯留時間
二、生長條件對FC-01細胞株生合成原花青素、鞣花酸和白藜蘆醇的影響
三、FC-01細胞株之萃取物對酪胺酸酶活性之抑制效能
柒、結論
捌、參考文獻

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