臺灣博碩士論文加值系統

本研究利用鹽酸降解幾丁聚醣(530KDa,Mv)，接著透過分餾作用使小分子量幾丁聚醣析出，當分餾區間pH 5.8-6.2時有最小分子量61KDa，而不同分餾區段有不同分子量分佈，接著利用幾丁聚醣與三聚磷酸鈉間之正負電荷作用進行離子交聯反應。為了找出最佳交聯條件使交聯微粒有最小粒徑，分別改變幾丁聚醣溶液pH值、CS/TPP比值，當pH值4及CS/TPP比值4時有最小粒徑，接著使用粒徑分析儀進行粒徑及介面電位測試，接著透過SEM觀察微粒大小分布情形並以AFM觀察微粒表面狀態。

進行包覆測試時，改變溶液pH值、牛血清蛋白濃度及幾丁聚醣分子量並探討各變因對包覆微粒粒徑及介面電位的影響，接著研究包覆率及負載率。包覆後微粒進行釋放實驗時，使用不同分子量之幾丁聚醣包覆牛血清蛋白後分別放入酸性及弱鹼性環境中，並研究分子量及不同環境與釋放速率的關係，而結果顯示在酸性環境中釋放速率較快，而分子量61KDa幾丁聚醣之交聯微粒有較好之釋放速率。

目 錄
中文摘要…………………………………………………………Ⅰ
目 錄…………………………………………………………Ⅱ
表 目 錄…………………………………………………………Ⅴ
圖 目 錄…………………………………………………………Ⅵ
壹、緒論 1
1-1 研究背景與動機……………………………………1
1-2 研究方法與目的……………………………………2
貳、文獻回顧…………………………………………………3
2-1生物可降解性高分子…………………………………3
2-2幾丁質與幾丁聚醣……………………………………5
2-3奈米藥物載體與釋放…………………………………11
2-4幾丁聚醣奈米微粒之製備……………………………19
2-5包覆牛血清蛋白微粒之製備…………………………25
参、實驗材料與方法…………………………………………29
3-1 實驗材料……………………………………………29
3-2 幾丁聚醣與微粒製備………………………………30
3-2-1幾丁聚醣之純化與製備………………………30
3-2-2幾丁聚醣的分餾………………………………31
3-2-3離子凝膠法製備幾丁聚醣微粒………………31
3-3 分子量測定………………………………………………31
3-3-1黏度分子量測試………………………………31
3-3-2凝膠滲透層析儀………………………………32
3-4去乙醯度測定…………………………………………32
3-5微結構分析……………………………………………34
3-5-1熱場發射掃描式電子顯微鏡…………………34
3-5-2 原子力顯微鏡…………………………………35
3-6粒徑分析儀……………………………………………37
3-7包覆率及負載率………………………………………38
3-8釋放速率………………………………………………40
肆、結果與討論…………………………………………………41
4-1幾丁聚醣結構分析……………………………………41
4-1-1幾丁聚醣分子量與分子量分佈………………41
4-2幾丁聚醣交聯微粒結構測試…………………………47
4-2-1 pH值對交聯微粒粒徑影響……………………47
4-2-2 CS/TPP比值對交聯微粒粒徑影響……………52
4-2-3分子量對交聯微粒粒徑影響……………………57
4-2-4原子力顯微鏡微結構分析………………………62
4-3包覆微粒性質探討………………………………………64
4-3-1 pH值對包覆微粒測試……………………………64
4-3-2 BSA濃度對包覆微粒測試………………………69
4-3-3分子量對包覆微粒測試…………………………74
4-4釋放速率
4-4-1酸性環境釋放速率…………………………………79
4-4-2弱鹼性環境釋放速率………………………………81
伍、結論……………………………………………………………83
陸、參考文獻………………………………………………………85

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