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研究生:趙文瑜
研究生(外文):Wen-Yu Chao
論文名稱:鹿茸萃取物對卵白蛋白誘導之呼吸道過度反應的調節作用
論文名稱(外文):Velvet antler extracts modulate OVA-induced airway hyperresponsiveness
指導教授:謝長奇謝長奇引用關係
指導教授(外文):Chang-Chi Hsieh
口試委員:林文川詹明修謝長奇
口試委員(外文):Wen-Chuan LinMing-Shiou JanChang-Chi Hsieh
口試日期:2011.06.10
學位類別:碩士
校院名稱:東海大學
系所名稱:畜產與生物科技學系
學門:農業科學學門
學類:畜牧學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:104
中文關鍵詞:呼吸道過度反應過敏體質免疫調節鹿茸
外文關鍵詞:Airway hyperresponsivenessAllergic constitutionsImmunomodulationVelvet antler
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過敏性氣喘為一種慢性呼吸道過度反應,常伴隨著肺部發炎、嗜酸性白血球浸潤與血清中IgE含量增加。已有研究指出鹿茸在體外試驗中可抑制大鼠腹股溝淋巴結生成IL-2,亦可抑制發炎物質前驅物的細胞激素例如IL-1β、IL-6、TNF-α。而臨床試驗發現,氣喘孩童在不停用西藥下,同時服用鹿茸後,血清中組織胺、白三烯素C4有下降的趨勢,且IFN-γ增加,但其相關機制不明。因此本研究希望能藉由動物模式來進一步探討鹿茸對於呼吸道過度反應之調節作用。試驗中以卵白蛋白致敏八週齡BALB/c雌鼠誘發其呼吸道過度反應,以口服方式餵食小鼠不同劑量之鹿茸酒精萃取物(50、100、300 mg/kg),並以皮質類固醇(10 mg/kg)作為正對照組。七週後測量其呼吸道阻力,並犧牲小鼠收集頸部氣管週邊淋巴結、肺部沖洗液、脾、胸腺、肺等,進行淋巴細胞表面標記分析(CD3, CD4, CD8, CD19, CD25, CD69, CD278, Tim-3)、抗卵白蛋白抗體(Anti-OVA-IgE, Anti-OVA-IgG1, Anti-OVA-IgG2a)及細胞激素(IL-4, IL-5, IFN-γ)測定、肺部沖洗液中血球形態區分、蘇木素與嗜伊紅染色法(Hematoxylin-eosin stain)和過碘酸希夫染色(Periodic acid-Schiff stain)評估肺部組織病理切片中細胞浸潤、氣管受損及黏液分泌情形。結果顯示經過卵白蛋白致敏後,鹿茸萃取物可增加肺部沖洗液中Th1細胞(Tim-3, CD4+)比例,減少脾臟淋巴細胞中Th2(CD278+, CD4+)細胞比例;且降低肺部沖洗液中IL-4與IL-5分泌量、降低血清及肺部沖洗液中抗卵白蛋白IgE與IgG1生成,提高其抗卵白蛋白IgG2a生成量;並減少肺部支氣管週邊細胞浸潤情形及嗜酸性白血球數量;減少肺泡壁發炎反應、靜脈週邊區域中、動脈週邊與支氣管週邊區域中發炎細胞浸潤;進而顯著性減緩呼吸道過度反應。進一步利用細胞試驗來解明分子調控之機轉。以鹿茸萃取物或鹿茸不同極性萃取層刺激EL4細胞株及RAW 264.7細胞株觀察其細胞激素的分泌調控。鹿茸萃取物(10.0 μg/ml)可顯著性提高EL4細胞的IFN-γ分泌量,而鹿茸氯仿萃取物可提升Raw 264.7細胞的IL-12分泌量。綜上所述,鹿茸可透過活化全身性之T調節細胞、調節naïve T細胞分化趨向Th1 cells、進而降低過敏性IgE抗體生成與發炎細胞於肺部浸潤來降低呼吸道過度反應。
Allergic asthma is a chronic disease associated with airway hyperresponsiveness such as bronchial inflammation, eosinophils infiltration and increase of serum IgE. Previous in vitro studies were reported that velvet antler can decrease the production of IL-2, and suppress pro-inflammatory cytokines including IL-1β, IL-6 and TNF-α. Otherwise, in the clinical study, it was discovered that the concentration of histamine and leucotriene were decreased and IFN- was increased in serum of asthmatic children after velvet antler treatment. But the relative mechanism is still unclear. In this study, we established the animal model to realize the effect of velvet antler (VA) in airway hyperresponsiveness. Female BALB/c mice were sensitized and challenged with ovalbumin (OVA) to induce airway hyperresponsiveness and administered with various doses of velvet antler (50, 100, and 300 mg/kg) or prednisolone (10 mg/kg) for seven weeks. After seven weeks treatments, mice were determined the enhanced pause (Penh) using whole body plethysmograpy, pulmonary cell infiltration and T cell differentiation, anti-OVA antibodies (including Anti-OVA-IgE, Anti-OVA-IgG1, Anti-OVA-IgG2a) and cytokines (such as IL-4, IL-5, IFN-γ) analysis, the paraffin section of lung for hematoxylin-eosin stain and periodic acid-Schiff stain. The results indicated that Velvet antler extracts (VAE) could increase the Th1 cells (Tim-3, CD4+) and reduce the Th2 cells (CD278+, CD4+) and to modulate T helper cell differentiation. VAE significantly decreased the secretion of IL-4 and IL-5 in bronchoalveolar lavage fluid (BALF) of asthmatic mice. Anti-OVA IgE and IgG1 were significantly decreased and anti-OVA IgG2a were significantly increased in serum and BALF of asthmatic mice. It also decreased the leukocytes infiltration and eosinophils counts in BALF. Moreover, VAE reduced the infiltration in perivenous regions and periarterial and periboronchial regions and inflammation in alveolar wall. VAE significantly decrease Penh after OVA challenged in allergic BALB/c mice. Furthermore, we used in vitro cell culture system to determinate the molecular regulation mechanism. Various doses of VAE or VAE fractions were treated EL4 cells and RAW 264.7 cells to observe their cytokines modulation. It was showed that VAE significantly raised the production of IFN-γ in EL4 cells. The chloroform fraction of VA stimulated the IL-12 expression in RAW 264.7 cells. It suggested that velvet antler might effectively modulate Treg cells, regulate T cells tend to th1 cells differentiation, down-regulated allergic IgE and inflammatory cells infiltration to regulated airway hyperresponsiveness.
壹、 摘要 1
貳、 前言 5
參、 文獻探討 7
一、 鹿茸 7
(一) 鹿茸來源與型態 7
(二) 鹿茸分類 8
(三) 鹿茸品質鑑別 8
(四) 鹿茸之組成份 9
(五) 鹿茸之藥理作用 10
(六) 鹿茸之毒性與副作用 11
二、 氣喘 12
(一) 氣喘定義 12
(二) 氣喘盛行率及死亡率 12
(三) 氣喘病理特徵 13
(四) 免疫系統 13
(五) 氣喘相關分子 14
(六) 氣喘相關細胞表面抗原分子介紹 16
(七) 氣喘致病機轉 17
(八) 氣喘治療 19
肆、 研究目的 21
伍、 材料方法 22
一、 實驗材料 22
二、 實驗方法 24
(一) 動物飼養與分組 24
(二) 鹿茸萃取物製備流程 24
(三) 動物致敏模式 25
(四) 樣品收集 25
(五) 樣品分析 26
(六) 以EL4細胞株和Raw264.7細胞株模擬體內細胞 29
陸、 統計分析 31
柒、 結果 32
一、 小鼠呼吸道過度反應之免疫調節 32
(一) 小鼠體重、脾臟、胸線與肺臟重量之變化 32
(二) 呼吸道過度反應中肺部沖洗液細胞總數之變化 32
(三) 呼吸道過度反應中呼吸道週邊淋巴細胞次族群之變化 32
(四) 呼吸道過度反應中肺部沖洗液淋巴細胞次族群之變化 33
(五) 呼吸道過度反應中脾臟淋巴細胞次族群之變化 34
(六) 呼吸道過度反應中肺部沖洗液細胞激素之變化 35
(七) 呼吸道過度反應中血清與肺部沖洗液特異性抗體之變化 35
(八) 呼吸道過度反應中肺部沖洗液細胞浸潤種類之變化 36
(九) 肺部組織病理切片之變化 36
(十) 小鼠呼吸道過度反應之變化 37
二、 以細胞模式模擬體內細胞反應 37
(一) EL4細胞株生長週期 37
(二) EL4細胞經鹿茸萃取物刺激後細胞增生之效果 37
(三) EL4細胞經鹿茸萃取物刺激後分泌細胞激素之變化 38
(四) EL4細胞經鹿茸不同極性萃取層刺激後細胞激素之變化 38
(五) Raw 264.7細胞經鹿茸不同極性萃取層刺激後細胞激素之變化 38
捌、 討論 39
一、 呼吸道過度反應中T細胞之分化 39
二、 呼吸道過度反應中IgE之生成 40
三、 呼吸道過度反應中經過免疫調節支氣管病理狀況 41
四、 以細胞模式模擬體內細胞 42
玖、 結論 43
壹拾、 參考文獻 44
壹拾壹、 附錄 51
壹拾貳、 附表 84
壹拾參、 附圖 88
壹拾肆、 附件 94

王志華、趙文忠、李二秋。2000。人參、鹿茸對體液免疫功能改善的研究。今日科技11:32-33。
王本祥與陳曉光。1989。次黃嘌呤對單胺氧化酶的抑制作用。藥學學報 8:573-577。
王琮仁。2004。分析進口鹿茸之氨基酸及銅鐵之含量。國立東華大學生物技術研究所碩士論文。
那琦與謝文全。1977。重輯名醫別錄。中國醫藥學院中國藥學研究所,台中。
李時珍。1982。本草綱目。人民衛生出版社,中國。
呂春美、黃曉薇、賴銘淙、李明憲、許清祥。2001。過敏性氣喘寒熱證型動物模型之建立。中國醫藥期刊12:155-164。
宋玉萍、李建勳、潘錫明。2000。中藥鹿茸的品種分類和外觀鑑定。中草藥 31(8):634-635。
凌雲、羅方成、李秋俠。1999。中藥鹿茸性激素的含量測定。中草藥 30(7):499。
高一瑛。1994。鹿茸、西洋蔘、黃芩、甘草及麻杏甘石湯。台北醫學大學生藥所研究所碩士論文。
孫茂峰。2010。中醫臨床標準作業程序及療效評估:以氣喘、腦中風、腦性麻痺為例(全程總報告)。中醫藥年報 28(3):69-110
馬志平。1992。人蔘、鹿茸、燕窩真偽品的簡易鑑別方法。福建中醫藥23(5):17。
唐巍然、聞傑、于曉紅、張德山。1998。鹿茸多醣對小鼠免疫功能的影響。中醫藥信息15(006):43-44。
唐巍然、于曉紅、聞傑、楊寶準、王桂山、張德山。2000。鹿茸多醣對免疫功能低下模型小鼠細胞免疫功能的影響。中國中醫藥科技7(004):234-234。
陳曉光、楊明、王木祥、曲文、崔勇與王岩。1990b。人工培養鹿茸細胞的藥理作用。中藥藥理與臨床 1:30-32。
陳俊吉。2004。老化與鹿茸給予對早老化小鼠骨骼動態及其相關內泌素變化之影響。東海大學畜產系研究所博士論文。
陳書明與聶向庭。2000。鹿茸醇提物對用環磷酰胺處理的小白鼠紅細胞免疫功能的影響。經濟動物學報4(001):23-25。
陳書明與聶向庭。2000。鹿茸醇提物對白細胞減少小鼠模型腹腔吞噬細胞吞噬功能的影響。山西中醫學院學報1(001):4-5。
陳朝宗。1998。腎氣丸對塵蹣激發氣喘天竺鼠呼吸道發炎與免疫之影響。中國醫藥學院中國醫學研究所碩士論文。
陳瑞楠。2001。鹿茸萃取液對鹿茸細胞增殖之影響。東海大學畜產系研究所碩士論文。
柴中元與施仁潮。2003。鹿茸,第198頁。浙江科學技術出版社,中國。
許清祥、徐昀耀、李明憲。2000。中醫平喘方劑對於過敏原特異性呼吸道發炎反應的作用機轉評估。中華醫誌11:111-121。
張賢哲與蔡貴花。1984。中藥炮製及藥材。中國醫藥學院出版組:855-859。
黃玉儒。2003。靈芝免疫調節蛋白的作用機制及其減緩塵致敏之應用。中山醫藥大學毒理學研究所碩士論文。
趙詠梅與陳曉光。2005。鹿茸口服液抗炎、鎮痛、免疫增強作用的實驗研究。中國中醫藥科技12:128-128。
鍾英傑。2005。梅花鹿茸的化學成分及藥理作用研究進展。長春中醫學院學報21(003):61-63。
羅翔丹,孫威,周玉,張鐵華,潘風光。2007。鹿茸活性多肽軟膠囊對大鼠免疫功效的初步研究。食品科學28(11):554-557。

Banks, W. J. 1974. The ossification process of the developing antler in the white-tailed deer (odocoileus virginianus). Calcif. Tissue Int. 14:257-274.
Barnes, P. J. and M. Karin. 1997. Nuclear factor-κB: a pivotal transcription factor in chronic inflammatory disease. New Eng. J. Med. 336:1066-1071.
Beck, L. and H. L. Spiegelberg. 1989. The polyclonal and antigen-specific IgE and IgG subclass response of mice injected with ovalbumin in alum or complete freund's adjuvant* 1. Cell. Immunol. 123:1-8.
Bousquet, J., P. Chanez, J. Y. Lacoste, G. Barneon, N. Ghavanian, I. Enander, P. Venge, S. Ahlstedt, J. Simony-Lafontaine and P. Godard. 1990. Eosinophilic inflammation in asthma. N. Engl. J. Med. 323:1033-1039.
Braitch, M., S. Harikrishnan, R. A. Robins, C. Nichols, A. J. Fahey, L. Showe and C. S. Constantinescu. 2009. Glucocorticoids increase CD4CD25 cell percentage and Foxp3 expression in patients with multiple sclerosis. Acta Neurol. Scand. 119:239-245.
Chen, J. Y. 1973. Chinese health foods and herb tonics. Am. J. Chin. Med. (Gard City. N. Y). 1:225-247.
Clifford, D. H., M.O. Lee, C.Y. Kim and D.C. Lee. 1979. Can an extract of deer antlers alter cardiovascular dynamics. Am. J. Chin. Med. 7:345-350.
Doganci, A., M. F. Neurath and S. Finotto. 2005. Mucosal immunoregulation: Transcription factors as possible therapeutic targets. Curr. Drug Targets Inflamm. Allergy. 4:565-575.
Feili-Hariri, M., D. H. Falkner and P. A. Morel. 2005. Polarization of naive T cells into Th1 or Th2 by distinct cytokine-driven murine dendritic cell populations: Implications for immunotherapy. J. Leukoc. Biol. 78:656-664.
Gould, H. J. and B. J. Sutton. 2008. IgE in allergy and asthma today. Nat. Rev. Immunol. 8:205-217.
Guidance, C. 1996. Guidance for industry. Center for Biologics Evaluation and Research (CBER).
Hamada, K., Y. Suzaki, A. Leme, T. Ito, K. Miyamoto, L. Kobzik and H. Kimura. 2007. Exposure of pregnant mice to an air pollutant aerosol increases asthma susceptibility in offspring. Journal of Toxicology and Environmental Health, Part A. 70:688-695.
Huang, S. L., N. Kakiuchi, M. Hattori and T. Namba. 1991. A new monitoring system of cultured myocardial cell motion: Effect of pilose antler extract and cardioactive agents on spontaneous beating of myocardial cell sheets. Chem. Pharm. Bull. (Tokyo). 39:384-387.
JinYuarn, L. and L. BiFong. 2000. Effects of weekly vs bi-weekly ovalbumin sensitization protocols in ICR mice. Food Science and Agricultural Chemistry. 2:28-34.
Kaminuma, O., A. Mori, N. Kitamura, T. Hashimoto, F. Kitamura, S. Inokuma and S. Miyatake. 2005. Role of GATA-3 in IL-5 gene transcription by CD4+ T cells of asthmatic patients. Int. Arch. Allergy Immunol. 137 Suppl 1:55-59.
Kao, S., S. Wang, C. Yu, H. Lei and J. Wang. 2000. The effect of chinese herbal medicine, xiao-qing-long tang (XQLT), on allergen-induced bronchial inflammation in mite-sensitized mice. Allergy. 55:1127-1133.
Kenyon, N. J., K. Gohil and J. A. Last. 2003. Susceptibility to ovalbumin-induced airway inflammation and fibrosis in inducible nitric oxide synthetase-deficient mice: Mechanisms and consequences. Toxicol. Appl. Pharmacol. 191:2-11.
Kim, K. H., K. S. Kim, B. J. Choi, K. H. Chung, Y. C. Chang, S. D. Lee, K. K. Park, H. M. Kim and C. H. Kim. 2005. Anti-bone resorption activity of deer antler aqua-acupunture, the pilose antler of cervus korean TEMMINCK var. mantchuricus swinhoe (nokyong) in adjuvant-induced arthritic rats. J. Ethnopharmacol. 96:497-506.
Kim, K. S., Y. H. Choi, K. H. Kim, Y. C. Lee, C. H. Kim, S. H. Moon, S. G. Kang and Y. G. Park. 2004. Protective and anti-arthritic effects of deer antler aqua-acupuncture (DAA), inhibiting dihydroorotate dehydrogenase, on phosphate ions-mediated chondrocyte apoptosis and rat collagen-induced arthritis. Int. Immunopharmacol. 4:963-973.
Kim, Y. K., K. S. Kim, K. H. Chung, J. G. Kim, K. S. Kim, Y. C. Lee, Y. C. Chang and C. H. Kim. 2003. Inhibitory effects of deer antler aqua-acupuncture, the pilose antler of cervus korean TEMMINCK var mantchuricus swinhoe, on type II collagen-induced arthritis in rats. Int. Immunopharmacol. 3:1001-1010.
Kiwamoto, T., Y. Ishii, Y. Morishima, K. Yoh, A. Maeda, K. Ishizaki, T. Iizuka, A. E. Hegab, Y. Matsuno, S. Homma, A. Nomura, T. Sakamoto, S. Takahashi and K. Sekizawa. 2006. Transcription factors T-bet and GATA-3 regulate development of airway remodeling. Am. J. Respir. Crit. Care Med. 174:142-151.
Kohguchi, M., T. Kunikata, H. Watanabe, N. Kudo, T. Shibuya, T. Ishihara, K. Iwaki, M. Ikeda, S. Fukuda and M. Kurimoto. 2004. Immuno-potentiating effects of the antler-shaped fruiting body of ganoderma lucidum (rokkaku-reishi). Biosci. Biotechnol. Biochem. 68:881-887.
Kuo, Y. C., W. J. Tsai, J. Y. Wang, S. C. Chang, C. Y. Lin and M. S. Shiao. 2001. Regulation of bronchoalveolar lavage fluids cell function by the immunomodulatory agents from cordyceps sinensis. Life Sci. 68:1067-1082.
Larche, M. 2007. Regulatory T cells in allergy and asthma. Chest. 132:1007-1014.
Lee, M. H., J. Park, S. W. Chung, B. Y. Kang, S. H. Kim and T. S. Kim. 2004. Enhancement of interleukin-4 production in activated CD4+ T cells by diphthalate plasticizers via increased NF-AT binding activity. Int. Arch. Allergy Immunol. 134:213-222.
Lefrancois, L. and N. Lycke. 2001. Isolation of mouse small intestinal intraepithelial lymphocytes, peyer's patch, and lamina propria cells. Curr. Protoc. Immunol. Chapter 3: Unit 3.19.
Li, Y. J., T. H. Kim, H. B. Kwak, Z. H. Lee, S. Y. Lee and G. J. Jhon. 2007. Chloroform extract of deer antler inhibits osteoclast differentiation and bone resorption. J. Ethnopharmacol. 113:191-198.
Lin, J. Y., M. L. Chen, B. L. Chiang and B. F. Lin. 2006. Ganoderma tsugae supplementation alleviates bronchoalveolar inflammation in an airway sensitization and challenge mouse model. Int. Immunopharmacol. 6:241-251.
Lin, J. Y., M. L. Chen and B. F. Lin. 2006. Ganoderma tsugae in vivo modulates Th1/Th2 and macrophage responses in an allergic murine model. Food Chem. Toxicol. 44:2025-2032.
Liu, Y. H., C. F. Tsai, M. C. Kao, Y. L. Lai and J. J. Tsai. 2003. Effectiveness of Dp2 nasal therapy for Dp2- induced airway inflammation in mice: Using oral ganoderma lucidum as an immunomodulator. J. Microbiol. Immunol. Infect. 36:236-242.
Mauri, C., M. Feldmann and R. O. Williams. 2003. Down-regulation of Th1-mediated pathology in experimental arthritis by stimulation of the Th2 arm of the immune response. Arthritis Rheum. 48:839-845.
Michael, A. M. and A. C. John. 2004. Coagulation-dependent mechanisms and asthma. J. Clin. Invest. 114: 20-3.
Motta, A. C., J. L. Vissers, R. Gras, B. C. Van Esch, A. J. Van Oosterhout and M. C. Nawijn. 2009. GITR signaling potentiates airway hyperresponsiveness by enhancing Th2 cell activity in a mouse model of asthma. Respir. Res. 10:93.
National Institutes of Health, National Heart, Lung, and Blood Institute. 1997. Expert Panel Report II. Guidelines for the diagnosis and management of asthma. NIH publication No. 97-4053.
Pan, H. J., H. X. Sun and Y. J. Pan. 2005. Adjuvant effect of ethanol extract of semen cuscutae on the immune responses to ovalbumin in mice. J. Ethnopharmacol. 99:99-103.
Park, J., S. W. Chung, S. H. Kim and T. S. Kim. 2006. Up-regulation of interleukin-4 production via NF-AT/AP-1 activation in T cells by biochanin A, a phytoestrogen and its metabolites. Toxicol. Appl. Pharmacol. 212:188-199.
Park, J., S. H. Kim, D. Cho and T. S. Kim. 2005. Formononetin, a phyto-oestrogen, and its metabolites up-regulate interleukin-4 production in activated T cells via increased AP-1 DNA binding activity. Immunology. 116:71-81.
Park, K. R., J. H. Lee, C. Choi, K. H. Liu, D. H. Seog, Y. H. Kim, D. E. Kim, C. H. Yun and S. S. Yea. 2007. Suppression of interleukin-2 gene expression by isoeugenol is mediated through down-regulation of NF-AT and NF-kappaB. Int. Immunopharmacol. 7:1251-1258.
Patel, M., D. Xu, P. Kewin, B. Choo-Kang, C. McSharry, N. C. Thomson and F. Y. Liew. 2005. Glucocorticoid-induced TNFR family-related protein (GITR) activation exacerbates murine asthma and collagen-induced arthritis. Eur. J. Immunol. 35:3581-3590.
Reagan-Shaw, S., M. Nihal and N. Ahmad. 2008. Dose translation from animal to human studies revisited. FASEB J. 22:659-661.
Romagnani, S. 2004. Immunologic influences on allergy and the TH1/TH2 balance. J. Allergy Clin. Immunol. 113:395-400.
Seminario, M. C. and G. J. Gleich. 1994. The role of eosinophils in the pathogenesis of asthma. Curr. Opin. Immunol. 6:860-864.
Shin, T. Y., S. H. Kim, K. Suk, J. H. Ha, I. Kim, M. G. Lee, C. D. Jun, S. Y. Kim, J. P. Lim, J. S. Eun, H. Y. Shin and H. M. Kim. 2005. Anti-allergic effects of lycopus lucidus on mast cell-mediated allergy model. Toxicol. Appl. Pharmacol. 209:255-262.
Suttie, J. M. and S. R. Haines. 1998 Evaluation of New Zealand vetvel antler efficacy and diagnostic testing. AgResearch.
Suh, S. J., K. S. Kim and A. Lee. 2007. Prevention of collagen-induced arthritis in mice by cervus korean TEMMINCK var. mantchuricus swinhoe. Environ. Toxicol. Pharmacol. 23:147-153.
Stock, P., T. Kallinich, O. Akbari, D. Quarcoo K. Gerhold U. Wahn, D. T. Umetsu and E. Hamelmann. 2004. CD8(+) T cells regulate immune responses in a murine model of allergen-induced sensitization and airway inflammation. Eur. J. Immunol. 34(7): 1817-27.
Sy, L. B., Y. L. Wu, B. L. Chiang, Y. H. Wang and W. M. Wu. 2006. Propolis extracts exhibit an immunoregulatory activity in an OVA-sensitized airway inflammatory animal model. Int. Immunopharmacol. 6:1053-1060.
Szabo, S. J., S. T. Kim, G. L. Costa, X. Zhang, C. G. Fathman and L. H. Glimcher. 2000. A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell. 100:655-669.
Takaki, H., K. Ichiyama, K. Koga, T. Chinen, G. Takaesu, Y. Sugiyama, S. Kato, A. Yoshimura and T. Kobayashi. 2008. STAT6 inhibits TGF-beta1-mediated Foxp3 induction through direct binding to the Foxp3 promoter, which is reverted by retinoic acid receptor. J. Biol. Chem. 283:14955-14962.
Takaki, H., K. Ichiyama, K. Koga, T. Chinen, G. Takaesu, Y. Sugiyama, S. Kato, A. Yoshimura and T. Kobayashi. 2008. STAT6 inhibits TGF-beta1-mediated Foxp3 induction through direct binding to the Foxp3 promoter, which is reverted by retinoic acid receptor. J. Biol. Chem. 283:14955-14962.
Tamachi, T., N. Watanabe, Y. Oya, S. Kagami, K. Hirose, Y. Saito, I. Iwamoto and H. Nakajima. 2007. B and T lymphocyte attenuator inhibits antigen-induced eosinophil recruitment into the airways. Int. Arch. Allergy Immunol. 143 Suppl 1:50-55.
Tykocinski, L. O., P. Hajkova, H. D. Chang, T. Stamm, O. Sozeri, M. Lohning, J. Hu-Li, U. Niesner, S. Kreher, B. Friedrich, C. Pannetier, G. Grutz, J. Walter, W. E. Paul and A. Radbruch. 2005. A critical control element for interleukin-4 memory expression in T helper lymphocytes. J. Biol. Chem. 280:28177-28185.
Umetsu, D. T. and R. H. DeKruyff. 2006. Immune dysregulation in asthma. Curr. Opin. Immunol. 18:727-732.
University of South Carolina. USA. http://pathmicro.med.sc.edu/bowers/cell-mediated-ver2.htm
Voetberg, B. J., B. A. Garvy, H. K. Mayer, L. E. King and P. J. Fraker. 1994. Apoptosis accompanies a change in the phenotypic distribution and functional capacity of murine bone marrow B-cells chronically exposed to prednisolone. Clin. Immunol. Immunopathol. 71:190-198.
Wang, B., X. Zhao, X. Yang, S. Kaneko, M. Hattori, T. Namba and Y. Nomura. 1988. Identification of the inhibitor for monoamine oxidase B in the extract from deer antler (rokujo). Journal of Medical and Pharmaceutical Society for WAKAN-YAKU. 5:116-122.
Wu, Y., H. Sun, F. Qin, Y. Pan and C. Sun. 2006. Effect of various extracts and a polysaccharide from the edible mycelia of cordyceps sinensis on cellular and humoral immune response against ovalbumin in mice. Phytother. Res. 20:646-652.
Xu, D., H. Liu, M. Komai-koma, C. McSharry, J. Alexander and F. Y. Liew. 2003. CD4+CD25+ regulatory T cells suppress differentiation and functions of Th1 and Th2 cells, Leishmania major infection, and colitis in mice. J. Immunol. 170(1): 394-9.
Xu, G., L. Cheng, L. Lu, Y. Zhu, R. Xu, X. Yao and H. Li. 2008. Expression of T-cell immunoglobulin- and mucin-domain-containing molecule-1 (TIM-1) is increased in a mouse model of asthma and relationship to GATA-3. Life Sci. 82:663-669.
Yamaguchi, S., M. Kagoshima, S. Kohge and M. Terasawa. 1997. Suppressive effects of Y-24180, a long-acting antagonist for platelet-activating factor, on allergic pulmonary eosinophilia in mice. Jpn. J. Pharmacol. 75:129.
Yamashita, N., H. Tashimo, H. Ishida, Y. Matsuo, H. Tamauchi, M. Terashima, I. Yoshiwara, S. Habu and K. Ohta. 2006. Involvement of GATA-3-dependent Th2 lymphocyte activation in airway hyperresponsiveness. Am. J. Physiol. Lung Cell. Mol. Physiol. 290:L1045-51.
Yudin, A. and Y. Dubryakov. 1974. A guide for the preparation and storage of uncalcified male antlers as a medicinal raw material. Reindeer antlers, Academy of Sciences of the USSR.Far East Science Centre.
Zavorotinskaya, T., A. Tomkinson and J. E. Murphy. 2003. Treatment of experimental asthma by long-term gene therapy directed against IL-4 and IL-13. Mol. Ther. 7:155-162.

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