(54.236.58.220) 您好!臺灣時間:2021/02/27 10:57
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:宋世雄
研究生(外文):Shih-Hsiung Sung
論文名稱:不分解蔗糖、麥芽糖或乳糖的酵母菌的篩選
論文名稱(外文):The Screening of Yeasts That Do Not Utilize Sucrose, Maltose or Lactose
指導教授:許垤棋
指導教授(外文):Dey-Chyi Sheu
學位類別:碩士
校院名稱:大同大學
系所名稱:生物工程學系(所)
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:57
中文關鍵詞:酵母菌寡糖
外文關鍵詞:oligosaccharidesyeast
相關次數:
  • 被引用被引用:1
  • 點閱點閱:828
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:1
有鑑於目前市售的低純度果寡糖、異麥芽寡糖和半乳寡糖糖漿內容物,除了寡醣的主成份外,大都還含有大量葡萄糖以及相當量的其它反應不完全的原料糖,例如:蔗糖、麥芽糖或乳糖等人體可消化糖,為了去除寡糖糖漿內的可消化糖,又不要減少寡醣的產量,達到純化出高純度寡糖漿的目的,因而想到在反應過程中,利用酵母菌對糖的醱酵具有選擇性,在不影響反應物的前題下以酵母菌醱酵法將反應過程中的葡萄糖去除,來提高產品中寡醣的含量,就可以提升寡醣的保健功能及商品價值,即篩選「不分解蔗糖、麥芽糖或乳糖的酵母菌」來達到此目標。將酵母菌培養於含有酵母萃取物、葡萄糖以及蔗糖、麥芽糖或乳糖的培養基中,然後用高效液相層析法分析殘存的醣類,以篩選出只消耗葡萄糖,而不分解蔗糖、麥芽糖或乳糖的酵母菌。在所篩選的酵母菌之中,Wickerhamomyces anomala BCRC 20858、Candida utilis BCRC 21990可代謝蔗糖,而不會利用麥芽糖和乳糖。另外,Pichia bimundalis BCRC 20479可輕微代謝乳糖,而Wickerhamomyces anomalus BCRC 20866則對於蔗糖、麥芽糖、乳糖都不會代謝。這些酵母菌都有潛力,將可利用於高純度寡糖的製造。
The low-content oligosaccharides including fructooligisaccharides (FOS), isomaltooligosaccharides (IMO) and galactooligosaccharides (GOS) consisted of not only oligosaccharides but also large amount of glucose and unreacted substrate sugars, such as sucrose, maltose and lactose. In order to eliminate these digestible sugars from oligosaccharides syrups and to increase the yields of oligosaccharides, selected yeasts were employed during the enzymatic reactions for the production of oligosaccharides. These yeasts should consume glucose alone but not the substrate sugars. During the oligosaccharides-forming reaction catalyzed by enzyme and yeast, the byproducts glucose was concurrently eliminated by yeast fermentation. This work was engaged in the screening of such yeasts that can ferment glucose but not sucrose, maltose or lactose. Yeasts were cultured in the fermentation medium composed of yeast extract, glucose and sucrose, maltose or lactose. Then the sugars in the fermented broth were analyzed by HPLC. Among four yeast strains, Wickerhamomyces anomala BCRC 20858 and Candida utilis BCRC 21990 could utilize sucrose but not maltose and lactose and Pichia bimundalis BCRC 20479 consume lactose slightly, whereas Wickerhamomyces anomalus BCRC 20866 did not utilize three sugars at all.
中文摘要 I
英文摘要 II
目錄 III
圖目錄 Ⅵ
表目錄 Ⅷ
第一章 緒論 1
1.1 前言 1
1.2 寡醣 2
1.2.1 何謂寡醣 2
1.2.2 寡醣的生理功能作用 4
1.2.3 寡醣研究應用的未來性 10
1.2.4 寡醣的製造 11
1.3 果寡醣 13
1.3.1 果寡醣的簡介 13
1.3.2 果寡醣的製造方式 16
1.3.2.1 果寡醣和高純度果寡醣的生成反應 17
1.3.2.2 生產高濃度果寡醣產品的方式 20
1.4 異麥芽寡醣 21
1.4.1異麥芽寡醣的簡介 21
1.4.2異麥芽寡醣的生產技術 25
1.5 半乳寡醣 27
1.5.1 半乳寡醣的簡介 27
1.5.2 半乳寡醣的製造方式 28
第二章 實驗材料、設備與方法 31
2.1 實驗材料 31
2.1.1 實驗菌種來源 31
2.1.2 菌種生長培養基所需之養分 31
2.2 設備 32
2.2.1. 一般設備 32
2.2.2. 分析設備 33
2.3 儀器分析原理 34
2.3.1 高效液相層析法(HPLC)的簡介 34
2.3.2 高效液相層析法的特點 35
2.3.3 Refraction Index Detector(RI偵檢器)原理 35
2.3.4 偵測器種類 36
2.4 實驗方法 37
2.5 糖液含量之分析方法 40
第三章 結果與討論 42
3.1 檢測各菌種對蔗糖sucrose及葡萄糖glucose的利用情形 42
3.2 檢測各菌種對麥芽糖maltose及葡萄糖glucose的利用情形 45
3.3 檢測各菌種對乳糖lactose及葡萄糖glucose的利用情形 48
第四章 結論 51
參考文獻 52
圖目錄
圖1.1 果寡醣 Fructooligosaccharides (FOS) 之化學結構圖 15
圖1.2 果寡醣和高純度果寡醣的生成反應 17
圖1.3 異麥芽寡醣主要成份的結構 22
圖1.4 市售異麥芽寡糖漿(環泰公司)所含各種糖的組成百分比 24
圖2.1 實驗流程圖 39
圖3.1 20479與蔗糖sucrose及葡萄糖glucose之HPLC層析圖譜 43
圖3.2 20858與蔗糖sucrose及葡萄糖glucose之HPLC層析圖譜 43
圖3.3 20866與蔗糖sucrose及葡萄糖glucose之HPLC層析圖譜 44
圖3.4 21990與蔗糖sucrose及葡萄糖glucose之HPLC層析圖譜 44
圖3.5 20479與麥芽糖maltose及葡萄糖glucose之HPLC層析圖譜 46
圖3.6 20858與麥芽糖maltose及葡萄糖glucose之HPLC層析圖譜 46
圖3.7 20866與麥芽糖maltose及葡萄糖glucose之HPLC層析圖譜 47
圖3.8 21990與麥芽糖maltose及葡萄糖glucose之HPLC層析圖譜 47
圖3.9 20479與乳糖lactose及葡萄糖glucose之HPLC層析圖譜 49
圖3.10 20858與乳糖lactose及葡萄糖glucose之HPLC層析圖譜 49
圖3.11 20866與乳糖lactose及葡萄糖glucose之HPLC層析圖譜 50
圖3.12 21990與乳糖lactose及葡萄糖glucose之HPLC層析圖譜 50
表目錄
表1.1 寡醣調節腸道蠕動及糞便重量之人體試驗 5
表1.2 目前商業生產的一些難消化寡醣之結構、製造方式及聚合度 11
表1.3 商業上半乳寡醣的製造方式的比較 28
表2.1 ICE-Sep ION-300標準品滯留時間(分)及積分面積(uv*sec) 41
表3.1 檢測各菌種對蔗糖sucrose及葡萄糖glucose的利用情形 42
表3.2 檢測各菌種對麥芽糖maltose及葡萄糖glucose的利用情形 45
表3.3 檢測各菌種對乳糖lactose及葡萄糖glucose的利用情形 48
表4.1 四株酵母菌對三種雙糖的利用情形 51
1.尤新,潘子明(2001)機能性發酵製品,第三章,低聚醣,藝軒圖書出版社;39-115.
2.大山武司作;徐玉枝、蕭淑君譯(1995)寡醣的效用:活化雙叉桿菌,預防現代病的食品,台北,青春出版社。
3.施淑銀(2008)半乳寡醣之特性與生產方式,食品工業,40卷,第6期;11-20.
4.孫安迪(2005)孫安迪教你解毒排毒,文經出版社;183-184;(2005)孫安迪之免疫處方,時報文化;(2009)孫安迪好好照顧你的腸道,邦聯文化。
5.陳曉鈴(2005)國家衛生研究院,論壇健康促進與疾病預防委員會,第五期,文獻回顧延析計畫-機能性食品成分對腸胃道功能的影響;31-49.
6.劉守芬,林怡希,蔡敬民(1994)生技合成難消化寡醣與聚糊精能改善成年男子排便與糞臭素之產生。中華民國營養學會雜誌;19:221-320.
7.尤新,潘子明(2001)機能性發酵製品,第三章,低聚醣,藝軒圖書出版社,39-115.
8.丁女娟(2008)高純度異麥芽寡糖的製造,碩士論文,大同大學生物工程研究所。
9.段國仁、許垤棋(2003)利用複合細胞系統製造高純度果寡糖,民國92年4月,知識創新第三十四期,P.3,生物技術產業。
10.畢家麟 (1996)複合生物催化系統生產高濃度果寡糖,碩士論文,大同大學生物工程研究所。
11.黃伯欣(2008)利用日本麴菌進行液態發酵和固態發酵生產果糖轉移酶,碩士論文,大同大學生物工程研究所。
12.鄭慈千(2005)固定化酵素生產果寡糖與半乳寡糖,博士論文,大同大學生物工程研究所。
13. Chen HL, Lu YH, Lin JJ(2001)Effects of isomalto-oligosaccharides on bowel functions and indicators of nutritional status in constipated elderly men. J Am Coll Nutr 20:44-90.
14. Cherbut C, Michel C, Lecannu G(2003) The prebiotic characteristics of fructooligosaccharides are necessary for reduction of TNBS-induced colitis in rats. J Nutr 133:21-7
15. Chicrici RG, Sawatzki S, Thurl K, Tovar K, Vigi V(1997). Experimental milk formula with reduced protein content and desialylated milk proteins:Influence on the faecal flora and the growth of term newborn infants. Acta paediatr 86:557-563.
16. Duan KJ, Sheu DC, Chen JS (1993) Purification and characterization of β-fructofuranosidase from Aspergillus japonicus TIT-KJ1. Biosci Biotech Biochem 57: 1811-1815.
17. Gibson GR, Roberfroid MB(1995)Dietary modulation of human colonic microbiota: introducing the concept of prebiotics, J Nutr 125:1401-1412.
18. Gibson GR(1999)Dietary modulation of the human gut microflora using the prebiotics oligofructose and inulin. J Nutr;129:1438S-41S.
19. Hamburger RN, Clemens RA, Brown C.(1997)The role of probiotics and prebiotics in infants. In J. A. Bellanti, R. Bracci, G. Prindull and M. Xanthou. ed. Neonatal Hematology and Immunology Ⅲ. Elsevier Science, Amsterdam. 159-172.
20. Harder A, Noordam, B, Brekelmans, AM(1983) Kinetic of isomation by amylogucosidase and purification of the disaccharide by fermentation of undesired by-products. Ann. N. Y. Sci., 413 : 340-351.
21. Hayashi, M. (1989) Effect of 4’-galactosyllactose on lipid metablism in the human serm. Med. Biol. 119:15-18.
22. Hayashi S, Imada K, Kushima Y, Ueno H. (1989) Oberservation of chemical structure of fructooligosaccharides produced by an enzyme from Aureobasidium sp. ATCC 20524. Current Microbiol. 19: 175-177.
23. Ishikawa F, Takayama H, Matsumoto K, Ito M, Chonan O, Deguchi Y, Kikuchi-Hayakawa H, Watanuki M (1995) Effects of β 1-4 linked galacto-oligosaccharides on human fecal microflora. Bifidus 9: 5-18.
24. Kaneko T, Kohmoto T, Kikuchi M(1994)Effects of isomaltooligosaccharides with different degrees of polymerisation on human faecal. Biotech Biochem 58:2288-2290.
25. Kohmoto T, Fukui F, Takaku H(1988)Effect of isomalto- oligosaccharides on human fecal flora. Bifid. Micro 7:61-69.
26. Loo J, Cummings J, Delzenne N(1999)Functional food properties of nondigestible oligosaccharides: a consensus report from the ENDO project. Br J Nutr 81:121-132.
27. MacGillivray PC, Finlay HVL, Binns TB(1959)Use of lactulose to create a predominance of lactobacilli in the intestine of bottle-fed infants. Scot Med J 4: 182-189.
28. Mahoney RR(1996)Lactose: enzymatic modification. In “Advanced Dairy Chemistry”, Fox, PF, Chapman and Hall, London. 3:77-126.
29. Mahoney RR(1998)Galactosyl-oligosaccharide formation during lactose hydrolysis - a review. Food chem. 63:147-154.
30. Matsumoto K, Kobayashi Y, Ueyama S, Watanabe T, Tanak R, Kan T, Kuroda A, Sumihara Y. chapter 5. Galactooligosaccharides. in: Nakakuki, T. ed. Oligosaccharides. Production, properties and applications. Japanese Technology Reviews, Vol. 3, no. 2, Gordon and Breach Science Publishers, Switzerland, Austrilia,(1993)90-106.
31. Sako T, Matsumoto K, Tanaka R(1999)Recent progress on research and application of non-digestible galacto- oligosaccharides. Int Dairy J 9:69-80.
32. Scholz-Ahrens KE, Schaafsma G, van den Heuvel EG (2001)Effects of prebiotics on mineral metabolism. Am J Clin Nutr 73: 459-464
33. Scholz-Ahrens KE, Schrezenmeir J(2002)Inulin, oligofructose and mineral metabolism-experimental data and mechanism. Br J Nutr 87: 179-186.
34. Takaku H(1988)Anomalously linked oligosaccharides mixture “Alomixture “ In The Amylase Research Society of Japan, Handbook of amylase and relative enzymes. Perg Pre Oxf 215-217.
35. Teuri U, Korpela R(1998) Galacto-oligosaccharides relieve constipation in elderly people. Ann Nutr Metab 42:319-327.
36. van den Heuvel EG, Muys T, van Dokkum W (1999) Oligofructose stimulates calcium absorption in adolescens.Am J Clin Nutr 69:544-548.
37. Voragen AGJ (1998)Technological aspects of functional food-related carbohydrates. Trends Food Sci Technol 9:328-335.
38. Wallenfels K, Malhotra OP(1961)Galactosidases. Adv Carbohydr Chem 16:239-298.
39. Wang HF, Lim PS, Kao MD (2001) Use of isomalto-oligosaccharide in the treatment of lipid profiles and constipation in hemodialysis patients. J Ren Nutr 11:73-79.
40. Younes H, Garleb K, Behr S(1995)Fermentable fibers or oligosaccharides reduce urinary nitrogen excretion by increasing urea disposal in the rat cecum. J Nutr 125:1010-1016
41. Yun JW, Lee MG, Song SK(1994) Continuous production of isomalto-oligosaccharides from maltose syrup by immobilized cells of permeabilized Aureobasidium pullulans. Biotech Lett 16:1145-1150.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔