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研究生:沈昕平
研究生(外文):Hsine-Ping Shen
論文名稱:利用電漿處理及UV光接枝聚合改善固定軟骨素於純鈦表面之研究
論文名稱(外文):IMMOBILISE CHITOSAN AND CHONDROITIN ON SURFACE MODIFIED TITANIUM SURFACE BY PLASMA TREATMENT AND UV LIGHT GRAFT
指導教授:陳克紹陳克紹引用關係
指導教授(外文):Ko-Shao Chen
學位類別:碩士
校院名稱:大同大學
系所名稱:材料工程研究所
學門:工程學門
學類:材料工程學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:94
中文關鍵詞:接枝軟骨素電漿
外文關鍵詞:graftchondroitinplasma
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鈦合金在植牙材與骨植入材上已經有相當廣泛的應用,而增進植牙材料表面與患部癒合為重要的議題,因此在純鈦表面上固定一層類細胞外基質之結構期望增進細胞的生長。但是金屬上沒有可固定生物分子之結構故本研究表面引入界面膜利用電漿處理即六甲基二矽氨烷( Hexamethyldisilazane,HMDSZ )電漿在植體表面沉積一層有機矽薄膜,可在其表面固定其他機能性高分子。又因有機矽膜為疏水性薄膜水接觸角約為90°,此疏水性質能改善抗蝕性。抗蝕電位經HMDSZ處理後從-0.41~ -0.07V,在HMDSZ 60W 60mtorr 10min 有最佳抗蝕性腐蝕電位約在-0.07 V。另外再使用氧氣(O2)電漿活化表面產生自由基,即可UV-light將丙烯酸(Acrylic Acid)單體接枝在電漿膜表面,使表面呈親水性且水接觸角約50°,此丙烯酸表面可使用EDC/NHS化學交聯將幾丁聚醣與軟骨素固定,固定率可達每平方公分3.83克。大鼠骨隨幹細胞培養結果顯示,未處理試片及氧氣親水化後之表面生長情形良好,細胞生長情形較佳,而疏水性之PD-HMDSZ表面及經AAc接枝以及固定幾丁聚醣和軟骨素之細胞貼附情形較差。
Titanium Alloy had been wide used in implants. Improve implant surface healing with wound is important discussion. The study is immbolised extracellular bone matrix (ECM) on the Titanium surface excepting that will improve cells growth. The study used plasma treatment of Hexamethyldisilazane ( HMDSZ) that treated on imapnt surface deposition organic silicon film.This organic silicon film contains carbon, nitrogen, oxygen functional groups that could be modified other functional groups. The organic silicon flim is hydrophobic that water contact angle is about 90°. This hydrophobic thin film could improve corrosion resistance. The corrosion volts after HMDSZ plasma treatment is about -0.41~-0.07V.HMDSZ plasma treated 60W 60mtorr 10min has the best corrosion volts is about -0.07V. Surface of oxygen plasma treated the HMDSZ thin film that generated radical.Surface of UV-light grafted Acrylic Acid (AAc) on the deposition film. The surface is hydrophilic. The water contact angle is about 50°. Surface of EDC/NHS cross linked chitosan and chondroitin on the surface.The graft density is about 3.83mg/cm2. Surface of the rats bone marrow stem cell culture, the untreated Titanium cell culture is well. O2 plasma treatmed PD-HMDSZ cell culture is well. After graft AAc and immobilise chitosan with chondroitin cell culture is not well.
致謝IV
摘要 V
Abstract VI
圖目錄ⅩI
表目錄ⅩI V
第一章 前言 1
1.1研究目的4
第二章 文獻回顧5
2.1植入性金屬合金5
2.2電漿及表面接枝聚合7
2.2.1電漿7
2.2.2低溫電漿表面處理10
2.2.3電漿表面處理在生醫醫材料上的應用13
2.2.4 UV光表面接枝聚合法16
2.3水膠介紹18
2.3.1網狀聚合物與膠體18
2.3.2水膠20
2.3.3植入性高分子21
2.3.4生物分解性高分子22
2.3.5固定生物性高分子22
2.3.6幾丁質與幾丁聚醣23
2.3.7軟骨素24
2.4腐蝕26
2.4.1腐蝕的定義與種類26
2.4.2極化曲線方法與原理29
第三章 實驗31
3.1 實驗步驟31
3.1.1 實驗材料31
3.1.2電漿沉積單體31
3.1.3 電漿沉積單體31
3.1.4表面接枝聚合水膠單體其添加物32
3.1.5交聯劑與耦合劑33
3.1.6生解性高分子34
3.2實驗設備35
3.3實驗流程36
3.3.1電漿沉積薄膜36
3.3.2電漿活化表面37
3.3.3UV光接枝聚合38
3.3.4利用化學交連固定幾丁聚醣或硫化軟骨素40
3.4細胞繼代培養40
3.5 實驗分析41
3.5.1水接觸角量測41
3.5.2接枝密度量測41
3.5.3 膜厚量測41
3.5.4場發射電子顯微鏡41
3.5.5表面官能基分析41
3.5.6 X光光電子能譜儀(ESCA)42
3.5.7細胞技術與存活測試.42
3.5.8體外細胞模擬試驗43
3.5.9 MTT assay44
3.5.10電化學分析45
第四章 結果與討論46
4.1水接觸角觀察46
4.2重量變化 49
4.3膜厚53
4.4 SEM表面形貌分析54
4.5 ESCA光譜儀表面鍵結分析55
4.6 細胞培養測試59
4.7電化學極化曲線測試63
5.結論70
參考文獻73
自述79
主要著作80
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