臺灣博碩士論文加值系統

隨著現代生活品質的提升，人類在醫療方面的要求也因應提高，在抽血檢驗方面，有時我們抽血檢驗時所需要血量並不多，如一般健康檢查時，許多檢查所需的血量多為1-5cc，如ESR(紅血球沉積速率)為1cc，抽血時人所感受的痛楚感除了在針頭進入人體時，還有抽血量的多寡，當抽血量愈多人體痛楚感受會愈大，所以隨著未來生物晶片檢測的多元化，以及檢測靈敏度的提升，將不需抽取大量血液進行後續分析，尤其當檢測元件微小化後整合於單一晶片上，即所謂Lab-on-a-chip。
本研究將提出介電泳晶片整合抽血裝置，達到在血液檢驗時不需多抽取血液就可達到檢驗效果，利用PDMS易翻模與良好的生物相容性，PDMS抽血機構以PDMS薄膜受壓時所產生的回復力抽取血液，故不須外加泵浦；實驗並使用微機電技術製作介電泳晶片，目的在於萃取血液中細胞。
實驗結果證實PDMS抽血機構可確實抽取豬血液為0.39cc，整合的介電泳晶片可成功捉取血液中紅血球，達成萃取細胞之目的，利用此方式製作此抽血機構可達到抽血時不需太多血量與不須倚靠醫護人員抽血技術即可達到抽血並且做初步萃取血球之目的。

Recent years, more and more patients need medical treatment. The healthy examination is important for people. The blood is drawn for medical examination purposes. Sometimes the patient doesn’t need to be drawn too much blood for examination, such like the ESR (The erythrocyte sedimentation rate) would draw only 1cc. The feeling of the hurt during the blood drawing is the time of drawing.
The development of microfluidic devices has become a popular research topic because of the advantages. They offer over conventional macroscale instruments including lower cost, lower sample and reagent consumption, adaptability for automation, and promise for portable point-of-care devices. With the development of the bioschips, and the increasing the sensitivity of the biosensors, it wouldn’t be drawn too much blood to analyze the result. When the biosensors are integrated in a chip, it calls Lab-on-a-chip.
This research proposes an integrated blood drawing and separating device. It needn’t draw too much blood to achieve the analyzing. This research proposes a novel mechanism to draw blood without external pump or vacuum tank, the deformation of PDMS(Polydimethylsiloxane) is used to achieve the purpose. The operation process of the blood drawing device with the deflection of the PDMS membrane is to generate the deformation of the volume. The DEP chip is also fabricated, it is in order to separate the cells of the blood.
For measuring the performance of the device, we also fabricate a PDMS tank as pig’s skin. The result of the pig’s blood drawn is 0.39cc, and the separating chip is also trapped the red cells of the blood. Finally, this research is demonstrated the drawing blood and separating purpose. It wouldn’t draw too much blood to analyze the result.

中文摘要 i
Abstract ii
誌謝 iii
目錄 iv
圖目錄 vii
表目錄 xii
PDMS薄膜力與撓曲關係符號說明 xiii
介電泳公式推導符號說明 xiv
第一章 序論 1
1.1 前言 1
第二章 文獻回顧 2
2.1抽血機構 2
2.1.1抽血機構相關文獻 2
2.2介電泳晶片 8
2.3文獻探討結論 12
第三章 設計原理 14
3.1 PDMS抽血機構 15
3.1.1研究目標 15
3.1.2 PDMS特性 17
3.1.3 PDMS抽血機構針頭選用 18
3.1.4 PDMS抽血機構設計 20
3.2介電泳晶片 36
3.2.1研究目標 36
3.2.2 電泳原理 36
3.2.3 介電泳原理 37
3.2.4介電泳分離原理 40
3.2.5數值模擬 43
3.2.6 光罩設計 48
3.2.7 電極設計 48
3.3儲血槽、仿皮膚儲血槽設計 49
第四章 實驗製作 52
4.1 PDMS抽血機構製作 53
4.2儲血槽、仿皮膚儲血槽製作 58
4.3介電泳電極製作 61
4.4晶片接合 64
5.1 PDMS抽血機構 66
5.1.1 PDMS抽血機構量測目的 66
5.1.2樣本製備 67
5.1.3 PDMS抽血機構量測 67
5.2 介電泳力量測 71
5.2.1量測系統架構 71
5.2.2樣品製備 73
5.2.3實驗步驟 75
5.2.4 介電泳力測量 76
5.3結果與討論 79
5.4 製程探討 80
第六章 結論與未來工作 82
6.1 結論 82
6.2 未來工作 83
參考文獻 84

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