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研究生:衛星妤
研究生(外文):Hsing -Yu Wei
論文名稱:松杉靈芝對熱中風抗發炎之效果
論文名稱(外文):THE ANTI-INFLAMMATORY EFFECTS OF GANODERMA TSUGAE ON THE HEATSTROKE OF RATS
指導教授:張林松張林松引用關係林錫斌林錫斌引用關係
指導教授(外文):Lin -Song ChangHsi-Pin Lin
學位類別:碩士
校院名稱:元培科技大學
系所名稱:食品科學研究所
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
畢業學年度:99
語文別:中文
論文頁數:66
中文關鍵詞:熱中風松杉靈芝免疫調節蛋白發炎反應組織損傷
外文關鍵詞:Heat strokeGanoderma tsugae immunomodulatory proteinInflammationTissue damage
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熱中風是長期處在41℃以上的高溫環境下,導致調節體溫失敗並產生高體溫且伴隨著中樞神經系統異常,如譫妄,抽搐或昏迷等症狀的疾病。目前可能的病理機制有(1)熱中風所產生的熱效應,可能會直接對器官組織和細胞造成損傷。組織和細胞損傷時,會分泌基質金屬蛋白酵素類別中的MMP2(負責重塑功能)與MMP9(負責分解細胞外基質)。MMP9分泌過多導致組織損傷嚴重。(2)體內產生過多的發炎介質 (TNF-α、IL-1β與一氧化氮),導致内毒素血症觸發全身性發炎反應,引起多器官功能衰竭。
松杉靈芝免疫調節蛋白(fungal immunomodulatory protein gts, FIP-gts)是從松杉靈芝菌絲體中純化出來,具有輔正體內過度免疫反應功能。熱中風可能會造成心臟、肝臟、肺臟、腎臟的損傷與衰竭。因此,利用FIP-gts改善熱中風大鼠,觀察FIP-gts是否能輔正心臟、肝臟、肺臟、腎臟組織過度的損傷與發炎反應,降低多器官功能衰竭與死亡的發生。
大鼠事前分別管餵兩週、四週、六週的FIP-gts,再誘導熱中風並紀錄誘導到死亡、誘導到生成、生成到死亡共三段的時間。利用酵素連結吸附分析法分析TNF-α、IL1-β與一氧化氮分析法分析一氧化氮表現量,觀察FIP-gts對熱中風大鼠併發的組織損傷與發炎反應,是否有輔正之功能。再利用西方墨點法分析MMP9、MMP2的分泌量與明膠酵素電泳分析活化程度,觀察FIP-gts對熱中風大鼠的組織損傷,是否有改善之功能。
結果顯示,持續給予兩週的FIP-gts可增加熱中風的存活時間。持續給予兩週、六週的FIP-gts可延遲熱中風生成的時間,進而使持續給予兩週、六週的FIP-gts大鼠在誘導熱中風到死亡的耐受性增強。事先持續餵食兩週、四週、六週的FIP-gts後,再誘導熱中風大鼠的心臟、肝臟、肺臟、腎臟對於產生的發炎介質 (TNF-α、IL1-β與一氧化氮)、MMP9的分泌量與活化程度會在不同週數下產生抑制作用,達到降低發炎反應與改善組織損傷之功效。MMP2分泌量與活化程度則無增加,因此FIP-gts無法增加細胞外基質重塑與修復組織的功能。
因此,FIP-gts可能藉由輔正熱中風體內組織過度的損傷與發炎反應,來延遲熱中風生成並延長熱中風後的存活時間。FIP-gts可能具有預防與改善熱中風所造成的傷害。


Heatstroke was induced by exposing rats to an ambient temperature of 41℃. It causes acute respiratory distress syndrome and myocardial injury. Severe cases lead to myocardial infarction and associated with central nervous system abnormalities, such as delirium, convulsions or coma and other symptoms of the disease.
FIP-gts, a fungal immunomodulatory protein found in Ganoderma tsugae, has been proposed to possess therapeutic effects on cancers and autoimmune diseases. Ganoderma tsugae is a biological activity of the protein and offers protection with its anti-inflammatory effects on heart, lung, and liver functions. The heatstroke caused by hyperthermia, the body's inflammatory response, and cardio-pulmonary disease put rats at higher risk. Therefore, it was the purpose of this study to use animal models of heatstroke to investigate whether FIP-gts can improve the heart, lung, liver and kidney inflammation due to heatstroke in rats. Rats were orally administered FIP-gts for 2 weeks, 4 weeks, and 6 weeks after the rats were exposed to an ambient temperature of 43℃ and induced the formation of heatstroke. The time of generated heatstroke (onset time) and heatstroke to the time of death (survival time) were recorded. Finally, the analysis of pro-inflammatory cytokines of TNF-α, IL1-β and nitric oxide by ELISA was done. Used western blot analysis method of MMP9, MMP2 secreted and activated of gelatin-zymography to observe the heatstroke of rats.
After 6 weeks of the oral FIP-gts, the onset time for heatstroke was delayed. In the oral FIP-gts 2 week group, the survival time was extended. In the oral FIP-gts 6 weeks group, the expression of TNF-α and IL1-β and nitric oxide and MMP9 enzyme was significantly decreased. These results suggest that the anti-inflammatory effects of FIP-gts can prolong the onset time and the survival time of heatstroke in correlation to a decrease in the expression of TNF-α, IL1-β, nitric oxide, and MMP9 enzyme in the multiorgan of rats.
damage. (2) Pro-inflammatory mediators produced in vivo (TNF-α, IL1-β and nitric oxide) is resulted in end toxemia trigger systemic inflammatory response syndrome, result the multiple organ failure.
Ganoderma tsugae immunomodulatory protein (fungal immunomodulatory protein gts, FIP-gts) from the mycelium of Ganoderma tsugae purified, have a function is auxiliary excessive immune response in vivo. Heat stroke caused heart, liver, lung, kidney damage and failure. Therefore, used the FIP-gts to improve the heat stroke rats, and observed the FIP-gts whether than auxiliary positive heart, liver, lung, kidney damage and excessive inflammation, to reduce multiple organ failure and death occur.
In the Rats before was feeding tubes for two weeks, four weeks, six weeks of FIP-gts, induced that heat stroke and record three paragraphs induced to death, and induced to produce, and produce to death. Used ELISA analysis method of TNF-α, IL1-β and nitric oxide analysis method, observed FIP-gts for the heat stroke complicated by tissue damage in rats with inflammation, whether have an auxiliary positive function. Used western blot analysis method of MMP9, MMP2 secreted and activated of gelatin-zymography to observe the FIP-gts heat stroke rats, tissue damage, whether have an improvement function.
The results showed that continued to give two weeks FIP-gts, heat stroke can increase the survival time. Continued to give two weeks, six weeks of FIP-gts, heat stroke can produce the time delay, thereby enabling the continued to give two weeks, six weeks of FIP-gts rats in the Induce of heat stroke to death of tolerated enhanced. Continued feeding prior two weeks, four weeks, six weeks of FIP-gts, then induced heat stroke rat heart, liver, lungs, kidneys, pro-inflammatory mediators(TNF-α, IL1-β and nitric oxide) and MMP9 enzyme’ secreted and activated, in different weeks, the produced inhibition, to reduce inflammation and improve the effect of tissue damage.
Thus, FIP-gts is possible by auxiliary heat stroke over in vivo tissue damage and inflammation, to delay heat stroke generation, survival time and tolerance induction to death. FIP-gts has a heat stroke prevention and to improve the effect.

誌謝 I
中文摘要 II
英文摘要 IV
目錄 VI
圖目錄 IX
表目錄 X
第一章 緒論 1
1.1 熱中風 1
1.2 發炎反應 4
1.3 基質金屬蛋白酶 5
1.4 松杉靈芝免疫調節蛋白 10
1.5 研究目的 11
第二章 實驗架構 12
第三章 材料與方法 13
3.1動物實驗 13
3.2實驗方法 13
3.2.1 實驗分組 13
3.2.2 心血功能及體溫之測定 14
3.2.3 熱中風誘發 14
3.2.4 蛋白質萃取 14
3.2.5 蛋白質含量與定量之測定 15
3.2.6 酵素連結免疫吸附方析法 16
3.2.7 一氧化氮分析 20
3.2.8 西方墨點法 21
3.2.9 明膠酵素電泳法 25
3.2.10 統計分析 26
第四章 結果 27
4.1心血功能及體溫之測定與熱中風誘發 27
4.2紀錄FIP-gts對大鼠在高溫誘導到死亡之耐受性、熱中風生成 ( Heat stroke on set time )與熱中風存活時間( survival time ) 27
4.2.1高溫誘導到死亡之耐受性 27
4.2.2 熱中風生成( Heat stroke on set time ) 28
4.2.3 熱中風存活時間( survival time ) 28
4.3檢測FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟降低發炎反應的效果 31
4.3.1檢測FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟的 TNF-α、IL1-β表現量 31
4.3.2檢測FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟的一
氧化氮表現量 37
4.4檢測FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟減少組織
損傷的效果 40
4.4.1檢測FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟的 Pro-MMP9 蛋白質表現量 40
4.4.2檢測FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟的
Pro-MMP2表現量 43
4.4.3檢測FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟的 Active-MMP9 蛋白質表現量 46
4.4.4檢測FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟的
Active-MMP2表現量 49
第五章 討論 52
5.1 FIP-gts對大鼠在高溫誘導到死亡之耐受性、熱中風生成 ( Heat stroke on set time )與熱中風存活時間( survival time )之影響 52
5.2 FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟的降低發炎反應的效果 53
5.2.1 FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟TNF-α、IL1-β表現量之影響 53
5.2.2 FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟一氧化氮表現量之影響 54
5.3 FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟的組織損傷 55
5.3.1 FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟的 Pro-MMP9、Pro-MMP2蛋白質表現量 55

5.3.2 FIP-gts對熱中風大鼠心臟、肝臟、肺臟、腎臟的 Active--MMP9、Active-MMP2活性表現量 57
第六章 結論 59
第七章 參考文獻 60
7.1 中文部分 60
7.2 英文部分 66

A. Bouchama, R. S. Parhar, A. el-Yazigi, K. Sheth, S. al-Sedairy,1991. Endotoxemia and release of tumor necrosis factor and interleukin 1 alpha in acute heatstroke. Journal of Applied Physiology, vol. 70, No.6, 2640-264.

Abderrezak Bouchama, M.D and James, P. Knochel, M.D, 2002. HEAT STROKE. N Engl J Med, Vol. 346, No. 25, 1978-1988.

Abigail L. Clutterbuck, Pat Harris, David Allaway, Ali Mobasheri, 2010. Matrix metallopr-
teinses in inflammatory pathologies of the horse. The Veterinary Journal, 183, 27–38.

A. H. Alzeer, A. Al-Arifi, A. S. Warsy, Z. Ansari H.Zhang, J.-L.Vincent, 1999. Nitric oxide production is enhanced in patients with heat stroke. Intensive Care Med , 25, 58- 62.

Akira Ito, Akihira Mukaiyama, Yoshifumi Itoh, Hideaki Nagase, Ida B. Thøgerseni, Jan J. Enghildi, Yasuyuki Sasaguri, Yo Mori,1996. Degradation of Interleukin 1β by Matrix Metall-
prteinases. THE JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 271, No. 25, Issue of June 21, pp. 14657–14660.

Anne Fouillet, Grégoire Rey, Françoise Laurent, Gérard Pavillon, Stéphanie Bellec, Chantal
Ghihenneuc-Jouyaux, Jacqueline Clavel, Eric Jougla, Denis Hémon, 2006. Excess mortality related to the August 2003 heat wave in France. Int Arch Occup Environ Health. 80(1), 16–
24.

Anne M. Manicone and John K. McGuire, 2008. Matrix Metalloproteinases as Modulators of Inflammation. Semin Cell Dev Biol, 19(1), 34–41.

Bhagwan S. Sanodiya, Gulab S. Thakur, Rakesh K. Baghel, G.B.K.S. Prasad, P.S. Bisen,2009. Ganoderma lucidum: A Potent Pharmacological Macrofungus. Current Pharmaceutical Biotechnology, 10, 717-742.

Charles B. Jones, David C. Sane, David M. Herrington, 2003. M atrix metalloproteinases: A review of their structure and role in acute coronary syndrome. Cardiovascular Research, 59, 812–823.
Chin Leong Lim and Laurel T. Mackinnon, 2006.The Roles of Exercise-Induced Immune System Disturbances in the Pathology of Heat Stroke, The Dual Pathway Model of Heat Stroke. Sports Med , 36 (1), 39-64.

Ching-Ping Chang, Chin-Cheng Lee, Sheng-Hsien Chen, Mao-Tsun Lin, 2004. Aminogua-
nidine Protects Against Intracranial Hypertension and Cerebral Ischemic Injury in Experim-
ental Heatstroke. J Pharmacol Sci 95, 56 – 64 .

David Leppert, Raija L.P. Lindber, Ludwig Kappos, Stephen L. Leib, 2001. Matrix metallo-
proeinases: multifunctional effectors of inflammation in multiple sclerosis and bacterial meningitis. Brain Research Reviews, 36, 249–257.

David Rodríguez , Charlotte J. Morrison, Christopher M. Overall, 2010. Matrix metalloprotei-
nases: What do they not do? New substrates and biological roles identified by murine models and proteomics. Biochimica et Biophysica Acta, 1803, 39–54.

Elise Lambert, Emilie Dassé, Bernard Haye, Emmanuelle Petitfrère, 2004. TIMPs as multifa-
cial proteins. Critical Reviews in Oncology/Hematology, 49, 187–198.

Elke Roeb, Iris Behrmann, Joachim Gro¨ Tzinger, Bettina Breuer, Siegfried Matern, 2000. An MMP-9 mutant without gelatinolytic activity as a novel TIMP-1-antagonist. The FASEB Journal, Vol. 14, 1671-1673.

Ghislain Opdenakker, Philippe E.Van den Steen, Jo Van Damme, 2001. Gelatinase B: a tuner and amplifier of immune functions. TRENDS in Immunology, Vol.22,No.10, 571-579.

Gilbert R. Upchurch, Jr, MD, John W. Ford, BA, Steven J. Weiss, MD, Brian S. Knipp, MA, David A. Peterson, BA, Robert W. Thompson, MD, Matthew J. Eagleton, MD, Autumn J. Broady, Mary C. Proctor, MS, James C. Stanley, MD, 2001. Nitric oxide inhibition increases matrix metalloproteinase–9 expression by rat aortic smooth muscle cells in vitro. JOURNAL OF VASCULAR SURGERY, Volume 34, Number 1, 76-83.

H. Grogun and P. M. Hopkins, 2002. Heat Stroke: implications for critical care and anaesthe-
sia. Birtih Journal of Anaesthesia. 88(5), 700-7.



Hyun-Jeong Ra and William C. Parks, 2007. Control of Matrix Metalloproteinase Catalytic Activity. Matrix Biol. 26(8), 587–596.

Ian M. Clark, Tracey E. Swingler, Clara L. Sampieri, Dylan R. Edwards, 2008. The regulation of matrix metalloproteinases and their inhibitors. The International Journal of Biochemistry & Cell Biology , 40, 62–1378.

IY Lee, CC Lee, CK Chang, CH Chien and MT Lin, 2005. Sheng mai san, a chinese herbal medicine, protects against renal ischaemic injury during Heat Stroke in the rat. Clinical and Experimental Pharmacology and Physiology. 32. 742–748.

James T. Wu and Lily L. Wu,2007. Local Inflammatory Diseases Are at Risk of Developing Myocardial Infarction, Stroke, Renal Failure and Cancer via Chronic Systemic Inflammation. J Biomed Lab Sci, Vol 19, No 2, 35-39.

Jiménez-Mejías ME, Montaño Díaz M, Villalonga J, Bollain Tienda E, López Pardo F, Pineda JA, González de la Puente MA,1990. Classical heatstroke in Spain. Analysis of a series 78 cases. Med Clin (Barc). 94(13). 481-486.

Kristina A. Szabo, Richard J. Ablin, Gurmit Singh, 2004. Matrix metalloproteinases and the immune response. Clinical and Applied Immunology Reviews , 4, 295–319.

Kun-Hung Shen, Chen-Kuei Chang, Mao-Tsun Lin, Ching-Ping Chang, 2008. Interleukin-1 receptor antagonist restores homeostatic function and limits multiorgan damage in heatstroke. Eur J Appl Physiol, 103, 561–568.

Lisa R. Leon, 2006. The thermoregulatory consequences of heat stroke:Are cytokines involved? Journal of Thermal Biology. 31. 67–81.

Lisa R. Leon and Bryan G. Helwig, 2010. Heat stroke: Role of the systemic inflammatory response. J Appl Physiol. VOL 109. 1980-1988.

Lisa R. Leon, Michael D. Blaha, and David A. DuBose, 2006. Time course of cytokine, corticosterone, and tissue injury responses in mice during heat strain recovery. J Appl Physiol, Vol 100, 1400–1409.


M. Corbel, E. Boichot, V. Lagente, 2000. Role of gelatinases MMP-2 and MMP-9 in tissue
remodeling following acute lung injury. Brealzaitliinaans eJosu arnnda l aocfu Mtee lduincga li najnudry Biological Research, 33, 749-754.

M. Kosaka, M. Yamane, R. Ogai, T. Kato, N. Ohnishi, E. Simon, 2004. Human body temperature regulation in extremely stressful environment: epidemiology and pathophysiolo-
gy of heat stroke. Journal of Thermal Biology, 29, 495–501.

Man-Hua Tong, Po-Jung Chien, Hui-Hsin Chang, Ming-Jer Tsai, Fuu Sheu, 2008. High Processing Tolerances of Immunomodulatory Proteins in Enoki and Reishi Mushrooms. J. Agric. Food Chem, 56, 3160–3166.

Magnus Bäck, Daniel F.J. Ketelhuth, Stefan Agewall, 2010. Matrix Metalloproteinases in Atherothrombosis. Progress in Cardiovascular Diseases, 52, 410–428.

Michael P. Murphy,1999. Nitric oxide and cell death. Biochimica et Biophysica Acta, 401-
414.

Mohammad-Fata Moradali, Hossein Mostafavi, Shirin Ghods, Ghorban-Ali Hedjaroude, 2007. Immunomodulating and anticancer agents in the realm of macromycetes fungi (macrofungi).
International Immunopharmacology, 7, 701–724.

Mohita J. Mohan, Theresa Seaton, Justin Mitchell, Anne Howe, Kevin Blackburn, William Burkhart, Mary Moyer, Inder Patel, Gregory M. Waitt, J. David Becherer, Marcia L. Moss, Marcos E. Milla, 2002. The Tumor Necrosis Factor-R Converting Enzyme (TACE): A Uniqu-
e Metalloproteinase with Highly Defined Substrate Selectivity. Biochemistry , 41, 9462-9469.

Nghia T.V. Le, Meilang Xue, Laura A Castelnoble and Christopher J. Jackson, 2007. The dual personalities of matrix metalloproteinases in inflammation. Frontiers in Bioscience, 12, 1475-1487.

PA´ L PACHER, JOSEPH S. BECKMAN, LUCAS LIAUDET, 2007. Nitric Oxide and Peroxynitrite in Health and Disease. Physiol Rev, 87, 315–424.

Ping Li, Edward M. Schwarz, 2003. The TNF-α transgenic mouse model of inflammatory arthritis. Springer Semin Immunopathol, 25, 19–33.

P Michaluk and L Kaczmarek, 2007. Matrix metalloproteinase-9 in glutamate-dependent
adult brain function and dysfunction. Cell Death and Differentiation, 14, 1255–1258.

R. H. Das, 2007. Nitric oxide in health & diseases. Indian J Med Res 125, 712-714.

Robert Visse, Hideaki Nagase, 2003. Matrix Metalloproteinases and Tissue Inhibitors of Met-
alloproteinases Structure, Function, and Biochemistry. Circulation Research, 92, 827-839.

Satoshi Hagiwara, Hideo Iwasaka, Chihiro Shingu, Shigekiyo Matsumoto, Tomohisa Uchida, Takayuki Noguchi, 2010. High-dose antithrombin III prevents heat stroke by attenuating
systemic inflammation in rats. Inflamm. Res. 59, 511–518.

Sebastian Pease, Lila Bouadma, Nathalie Kermarrec, Fre´de´rique Schortgen, Bernard Re´gnier, Michel Wolff, 2009. Early organ dysfunction course, cooling time
and outcome in classic heatstroke. Intensive Care Med. 35. 1454–1458.

Shizuko Tanaka, Kerry KO, Kohsuke Kino$, Kazuko Tsuchiya, Akio Yamashita, Akira Murasugi, Sadatoshi Sakuma, and Hajime Tsunoo, 1989. Complete Amino Acid Sequence oafn Immunomodulatory Protein, Ling Zhi-8 (LZ-8). THEJ OURNAOLF BIOLOGICACLH EMISTRY, Vol. 264, No. 28, 16372-16377.

Steven B. Abramson, Ashok R. Amin, Rober M. Clancy, Makhundan Attur, 2001. The role of nitric oxide in tissue destruction. Best Practice & Research Clinical Rheumatology. Vol. 15, No. 5, 831–845.

Tracy L. Adair-Kirk and Robert M. Senior, 2008. Fragments of Extracellular Matrix as Mediators of Inflammation. Int J Biochem Cell Biol, 40(6-7), 1101–1110.

Theresa Pluth Yeo, MSN, MPH, CRNP, 2004. Heat Stroke A Comprehensive Review. AACN Clinical Issues, Vol. 15, No. 2, 280–293.

T.J. Guzik, R. Korbut, T. Adamek-Guzik, 2003. Nitric Oxide and superoxide in inflammation
and immune regulation. Journal of physiology and pharmacology, 54, 4, 469-487.




Won-Shiung Liu, Chun-Ta Chen, Ning-Hui Foo, Hsuan-Rong Huang, Jhi-Joung Wang, Sheng-Hsien Chen, Te-Jen Chen, 2009. Human Umbilical Cord Blood Cells Protect Against Hypothalamic Apoptosis and Systemic Inflammation Response During Heatstroke in Rats. Pediatr Neonatol . 50(5). 208−216.

Xuan Zhang and Warren B. Nothnick, 2005. The role and regulation of the uterine matrix metalloproteinase system in mensyruating and non-menstruating species. Frontiers in Bioscience, 10, 353-366.

You-E Yan, Yong-Qi Zhao, Hui Wang, Ming Fan, 2006. Pathophysiological factors underlying heatstroke. Medical Hypotheses, 67, 609–617.

Yu-Li Lin, Yu-Chih Liang, Yu-Shan Tseng, Hsin-Yi Huang, Shu-Yu Chou, Ruey-Shyang Hseu, Ching-Tsan Huang, Bor-Luen Chiang, 2009. An immunomodulatory protein, Ling Zhi-8, induced activation and maturation of human monocyte-derived dendritic cells by the
NF-kB and MAPK pathways. Journal of Leukocyte Biology, Volume 86, 887-889.

王文賢,真菌類免疫調節功能蛋白抗發炎及穩定性之研究,中山醫學大學醫學分子毒理學研究所碩士論文,民國94年。

洪永瀚與林壁鳳,以巨噬細胞株分泌發炎介質篩選抗發炎作用的模式評估,國立台灣大學微生物生化研究所,臺灣營養學會雜誌,第29期第3卷,民國93年,第159-167頁。

柯俊良,評估靈芝免疫調節功能蛋白抑制肺癌細胞轉移能力,中醫藥年報 第26 期 第2 冊,民國97年。

許瑞祥,靈芝在生技領域研發的新趨勢,農業生技產業季,第三期,民國94年。

黃玉儒,靈芝免疫調節蛋白的作用機制及其減緩塵蟎致敏之應用,中山醫學大學醫學分子毒理學研究所碩士論文,民國92年。

葉娟美,重組靈芝免疫調節蛋白之食品級生產系統研發及應用潛力。農業生技產業季刊,食品生技, 第18卷,民國98年。

黃敏瑜,靈芝蛋白LZ-8抗發炎活性的探討,輔仁大學生命科學研究所碩士論文,民國94年。

童麗珠、葉雯芳、羅惠如,神經修護與保護,科學教育月刊,第310期,民國97年7月。

廖韋凱 林家伃 黃文濤 施科念,中草藥對免疫系統的生物效應,加馬第四十期 民國98年6月 第60-65 頁。

張林松,大白鼠熱中風時腦中葡萄糖利用率及轉運體之變化,國立陽明大學生理學研究所博士論文,名國89年。




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