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研究生:邱挺瑋
研究生(外文):Ting-Wei Chiu
論文名稱:常壓非熱電漿對大腸桿菌之滅菌效果評估
論文名稱(外文):Inactivation of E. coli by nonthermal atmospheric plasma
指導教授:洪雪芬洪雪芬引用關係
指導教授(外文):Hsueh-Fen Hung
學位類別:碩士
校院名稱:元培科技大學
系所名稱:環境工程衛生研究所
學門:工程學門
學類:環境工程學類
論文種類:學術論文
畢業學年度:99
語文別:中文
論文頁數:86
中文關鍵詞:介電質放電滅菌技術大腸桿菌臭氧
外文關鍵詞:Dielectric barrier dischargeDisinfectionInactivationE.coliOzone
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傳統滅菌方法包含物理以及化學性,但美中不足的地方為這些滅菌方法仍然受到些許條件之限制。電漿技術被視為符合改善傳統滅菌技術之一。電漿滅菌可分為常壓以及非常壓兩大類,非常壓電漿,需要在真空系統下產生電漿狀態,除設備複雜外耗能以及溫度也較高。而常壓電漿具有低溫且可在常壓下操作等優點,本研究利用常壓非熱電漿系統,包含針盤式介電質電漿(電漿表面殺菌式)系統以及電漿產生臭氧式系統對滅菌進行探討可能影響之原因。
本研究利用電漿表面殺菌式測試系統對滅菌效果之評估,以大腸桿菌為測試菌種,並以濾紙以及瓊脂培養基做為底襯,使用放射頻率60 Hz、電功率0至18.5 W,測試時間分別由0至10分鐘。由實驗結果發現滅菌效果不僅受時間影響,初始菌數濃度亦為重要因素之一。初始菌數濃度3 orders,處理時間為3分鐘即可達到完全滅菌,但菌數濃度提升至6 orders,滅菌效果僅有1 order。此外,電壓與電功率也扮演著重要的角色,處理時間為3分鍾,初始菌數濃度5 orders,17 kv滅菌效果僅有0.7 order,隨電壓強度增加至20 kv,其滅菌效果增加至2 orders。而通入氣體種類不同也會影響電漿滅菌效果,當電功率8 W時,通入氮氣、空氣、氧氣之滅菌效果分別為0、1、及3 orders。若以耗能基準比較系統之滅菌效率,則電漿產生臭氧式較電漿表面殺菌式具有優勢,電功率為8 W時,電漿產生臭氧式可去除6 orders,電漿表面殺菌式僅1 order。

Conventional sterilization techniques have some disadvantages such as limited use on heat-sensitive materials and time-consuming process of heating methods, residual toxicity and environmental pollution caused by the application of biocides and potential radiation hazards of irradiation sterilizations. In recent years, many researchers are devoted to develop novel disinfection techniques as an alternative to the traditional methods. Plasma has been wildly studied for its effectiveness and application on microbial inactivation. Based on the operating condition, gas plasma device can be divided into two categories. One is low pressure plasma device that can achieve a high efficiency of sterilization but has high cost on operation and equipment set-up. The other system, atmospheric plasma generating gas plasma at normal ambient pressure, is less complexity and thus has low cost on operation.

This study described the first application of a multi-needle-to-plate type DBD which can produce nonthermal atmospheric plasma on surface decontamination. A commonly used reference strain, Escherichia coli, deposited on membrane filters with agar medium was chosen to evaluate the inactivation performance of the DBD system operated at a low frequency of 60 Hz and a peak electric power of 18.5 W. It was found that the disinfection efficacy was not only treatment time-dependent but also strongly affected by cell loading density. An initial cell density of 2.3× 103 CFU/cm2 can be completely inactivated within 3 min by this DBD device. Nevertheless, only a count reduction of 1 log was reached while the cell density rise to 2.8×106 CFU/cm2. In addition, the supply voltage is another factor influencing the disinfection of E. coli. After 3 min of plasma treatment, a reduction in cell counts exceeding 2 log orders was observed at a voltage of 20 kV compared with a value of 0.7 log reduction at 18 kV (initial cell density 2.0 × 105 CFU/cm2 ). Compared with N2 and air, O2 used as the feeding gas can achieve a higher disinfection efficacy. The energy consumption of the plasma inactivation by the multi-needle-to-plate type DBD was lower than traditional plate type DBD.

致謝 II
中文摘要 III
英文摘要 IV
目錄 VI
表目錄 VIII
圖目錄 IX

第一章 前言 1
1.1 研究緣起 1
1.2 研究目的 1

第二章 文獻回顧 2
2.1 常用之消毒及滅菌方式 2
2.2 電漿 4
2.3 影響電漿表面殺菌之因素 8
2.4 電漿非表面殺菌 12

第三章 實驗設備與方法 21
3.1 微生物測試樣本之製備 21
3.2 電漿設備 24
3.3 菌落計數 26
3.4 霍氏紅外線吸收光譜儀(FTIR) 27

第四章 結果與討論 39
4.1 震盪過程對菌數回收之影響 39
4.2 吸光值(OD值)與菌數濃度之關係 39
4.3 電壓與輸出能量之關係 41
4.4 電壓強度對滅菌效果之影響 42
4.5 電漿處理時間對滅菌效果之影響 42
4.6 測試菌數濃度對電漿滅菌效果之影響 43
4.7 單位面積所施予之能量對滅菌效果的影響 44
4.8 氣體流量對滅菌效果之影響 45
4.9 探討不同電漿系統之滅菌效果 45
4.10 不同電漿系統在不同電功率下之滅菌效果 46
4.11 不同電漿系統在不同時間下之滅菌效果 47
4.12 電漿產生臭氧式系統通入不同氣體之滅菌效果 48
4.13 電漿表面殺菌式通入不同氣體之滅菌影響 49

第五章 結論 70

參考文獻 71


中文文獻
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(3) 徐詮亮、高孟誠,「電漿殺菌技術原理及其應用」,真空科技第十七卷第一期,2004。
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(5) 陳柏賢,「電漿技術於生物感測器表面改質與高分子材料表面殺菌之研究」,中國文化大學材料科學與製造研究所碩士論文,2004。
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