(3.231.166.56) 您好!臺灣時間:2021/03/08 11:25
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:張祐銓
研究生(外文):Yu-Chen Chang
論文名稱:Cep55蛋白質在小鼠睪丸細胞之分布及其被p53調控之機制
論文名稱(外文):Cep55 subcellular distribution in mouse testes and its regulation mechanism by p53
指導教授:歐陽品
指導教授(外文):P. Ouyang
學位類別:博士
校院名稱:長庚大學
系所名稱:臨床醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
論文頁數:103
中文關鍵詞:Cep55蛋白質細胞間橋p53蛋白質Plk1蛋白質
外文關鍵詞:Cep55intercellular bridgep53Plk1
相關次數:
  • 被引用被引用:0
  • 點閱點閱:204
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:28
  • 收藏至我的研究室書目清單書目收藏:0
Cep55蛋白質對於細胞分裂的完成很重要,它在間期(interphase)細胞會存在於中心體(centrosome),而在胞質分裂(cytokinesis)時則會存在於中體(midbody)。先前的北方墨點實驗顯示Cep55在睪丸會大量表現且在多數的人類癌症中可以發現Cep55蛋白質會過度表現與p53蛋白質的失去功能,這引起我們討論這兩個基因相關的可能性。在這個論文中我們首先檢驗在老鼠睪丸成熟過程中Cep55蛋白質的空間與時間的表現形態,其次我們評估p53對於Cep55調控的角色。我們發現Cep55和另一個中心體蛋白質pericentrin會存在於連接生殖細胞(spermatogenic cells)之細胞間橋(intercellular bridges)。我們也發現誘發p53生成會同時抑制Cep55和Plk1蛋白質之表現。Plk1的過度表現與抑制p53表現(knockdown)兩者皆會增強Cep55轉譯後(post-translational)之蛋白質穩定度。我們的結果顯示在精子生成(spermatogenesis)過程中需要Cep55和pericentrin形成生殖細胞間之穩定細胞間橋。我們也發現p53會經由Plk1負向調控Cep55之表現,而Plk1本身則會正向調控Cep55之蛋白質穩定度,這些資料顯示有p53-Plk1-Cep55調控軸的存在。
Centrosomal protein 55 (Cep55), located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. Northern blot previously showed Cep55 is predominantly expressed in the testis. Upregulation of Cep55 and inactivation of p53 occur in the majority of human cancers, raising the possibility of a link between these two genes. In the present thesis, we first examined the spatial and temporal expression patterns of Cep55 during mouse testis maturation and then we evaluated the role of p53 in Cep55 regulation. We found that Cep55, together with pericentrin, another centrosomal protein, were localized to the intercellular bridges interconnecting spermatogenic cells in a syncytium. We also demonstrated that downregulation of expression of Cep55 was accompanied by repression of polo-like kinase 1 (Plk1) levels due to p53 induction. Overexpression of Plk1 and knockdown of p53 expression both enhanced the post-translational protein stability of Cep55. Our results, therefore, indicate Cep55 and pericentrin are required for the stable bridge between germ cells during spermatogenesis and demonstrate the existence of a p53-Plk1-Cep55 axis in which p53 negatively regulates expression of Cep55 through Plk1 which, in turn, is a positive regulator of Cep55 protein stability.
TABLE OF CONTENTS
指導教授推薦書
口試委員審定書
國家圖書館授權書
長庚大學授權書
誌謝 i
摘要 ii
ABSTRACT iii
TABLE OF CONTENTS iv
FIGURES vi
CHAPTER I INTRODUCTION 1
1.1 Centrosomal Protein 55 kDa (Cep55) 1
1.1.1 Identification of Cep55 1
1.1.2 Gene functions of Cep55 3
1.1.3 Cep55 and cancers 5
1.2 Centrosome, cytokinesis, midbody, and intercellular bridges (IBs) 7
1.3 Testis and spermatogenesis 9
1.4 p53 10
1.5 Specific aims 11
CHAPTER II MATERIALS AND METHODS 13
PART I 13
2.1.1 Antibody production 13
2.1.2 Northern blot analysis 14
2.1.3 Western blot analysis 14
2.1.4 Cell culture and synchronization 15
2.1.5 Immunohistochemistry 16
2.1.6 Preparation of enzyme-dissociated testicular cells 17
2.1.7 Immunofluorescence microscopy 18
PART II 19
2.2.1 Cell culture, plasmids, and transfection 19
2.2.2 Growth rate assay 20
2.2.3 UV irradiation 20
2.2.4 Luciferase and -galactosidase assays 20
2.2.5 Cell treatment and Western blot analysis 21
CHAPTER III RESULTS 23
PART I 23
3.1.1 Characterization of monoclonal anti-Cep55 antibodies 23
3.1.2 Expression analysis of Cep55 in developing and adult testes 24
3.1.3 Localization of Cep55 to germ cell intercellular bridges in adult murine testes 26
3.1.4 Coexpression of Cep55 as well as pericentrin with MKLP1 at germ cell intercellular bridges in developing and adult testes 27
3.1.5 Cep55 and pericentrin form an inner ring in stable intercellular bridges from dissociated male germ cells 29
PART II 29
3.2.1 Correlation of Cep55 protein expression levels and ovarian cancer cell growth 29
3.2.2 Cep55 expression is negatively regulated by p53 31
3.2.3 Stability of Cep55 protein is influenced by p53 33
3.2.4 Repression of Cep55 by p53 is through Plk1 34
CHAPTER IV DISCUSSION 37
PART I - Characterization of centrosomal proteins Cep55 and Pericentrin in intercellular bridges of mouse testes 37
PART II - Cep55 stability is negatively regulated by p53 through Plk1 43
CHAPTER V SUMMARY 51
REFERENCES 80
APPENDIX 92


FIGURES
Fig. 1. Model for Cep55 phosphorylation during mitosis. 54
Fig. 2. Spermatogenesis in testis. 55
Fig. 3. Mouse stages in the cycle of the seminiferous epithelium (I-XII). 56
Fig. 4. Characterization of Cep55 monoclonal antibodies (mAbs). 58
Fig. 5. Expression of Cep55 in adult and developing testes. 59
Fig. 6. Immunohistological localization of Cep55 in adult mouse testis. 61
Fig. 7. Subcellular distribution of Cep55, MKLP1, and pericentrin in quiescent and dividing HeLa cells. 62
Fig. 8. Subcellular localizations of Cep55, MKLP-1, and pericentrin in mouse testes at different maturation stages to adult. 64
Fig. 9. Immunofluorescent localization of Cep55, MKLP-1, and pericentrin in dissociated cells from adult mouse testis. 65
Fig. 10. Cep55 expression contributes to cell proliferation. 67
Fig. 11. Repression of Cep55 protein expression in response to p53 induction. 69
Fig. 12. Cep55 promoter activity is suppressed by expression of p53. 71
Fig. 13. Cep55 stability is influenced by p53. 72
Fig. 14. Plk1 regulates Cep55 expression and protein stability. 74
Fig. 15. p53-dependent Cep55 expression is mediated by Plk1 phosphorylation. 77
Fig. 16. Expression patterns of Cep55 and Plk1 in isogenic cell lines with or without p53 expression. 78
Fig. 17. Model for regulatory pathway of Cep55. 79


Alastalo TP, Lonnstrom M, Leppa S, Kaarniranta K, Pelto-Huikko M, Sistonen L, Parvinen M (1998) Stage-specific expression and cellular localization of the heat shock factor 2 isoforms in the rat seminiferous epithelium. Experimental Cell Research 240:16-27

Böhlig L, Rother K (2011) One Function—Multiple Mechanisms: The Manifold Activities of p53 as a Transcriptional Repressor. Journal of Biomedicine & Biotechnology 2011:464916

Bartek J, Falck J, Lukas J (2001) CHK2 kinase--a busy messenger. Nature Reviews Molecular Cell Biology 2:877-886

Bartek J, Lukas J (2001) Mammalian G1- and S-phase checkpoints in response to DNA damage. Current Opinion in Cell Biology 13:738-747

Bastos RN, Barr FA (2010) Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission. Journal of Cell Biology 191:751-760

Bellve AR, Cavicchia JC, Millette CF, O'Brien DA, Bhatnagar YM, Dym M (1977) Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization. Journal of Cell Biology 74:68-85

Braun RE, Behringer RR, Peschon JJ, Brinster RL, Palmiter RD (1989) Genetically haploid spermatids are phenotypically diploid. Nature 337:373-376

Burgos MH, Fawcett DW (1955) Studies on the fine structure of the mammalian testis. I. Differentiation of the spermatids in the cat (Felis domestica). Journal of Biophysical & Biochemical Cytology 1:287-300

Canman JC, Wells WA (2004) Rappaport furrows on our minds: the ASCB Cytokinesis Meeting Burlington, VT July 22-25, 2004. Journal of Cell Biology 166:943-948

Carlton JG, Agromayor M, Martin-Serrano J (2008) Differential requirements for Alix and ESCRT-III in cytokinesis and HIV-1 release. Proceedings of the National Academy of Sciences of the United States of America 105:10541-10546

Carlton JG, Martin-Serrano J (2007) Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery. Science 316:1908-1912

Carmena M, Riparbelli MG, Minestrini G, Tavares AM, Adams R, Callaini G, Glover DM (1998) Drosophila polo kinase is required for cytokinesis. Journal of Cell Biology 143:659-671

Chang YC, Chen YJ, Wu CH, Wu YC, Yen TC, Ouyang P (2010) Characterization of centrosomal proteins Cep55 and pericentrin in intercellular bridges of mouse testes. Journal of Cellular Biochemistry 109:1274-1285

Chen CH, Chien CY, Huang CC, Hwang CF, Chuang HC, Fang FM, Huang HY, Chen CM, Liu HL, Huang CY, Huang CYF (2009a) Expression of FLJ10540 is correlated with aggressiveness of oral cavity squamous cell carcinoma by stimulating cell migration and invasion through increased FOXM1 and MMP-2 activity. Oncogene 28:2723-2737

Chen CH, Lai JM, Chou TY, Chen CY, Su LJ, Lee YC, Cheng TS, Hong YR, Chou CK, Whang-Peng J, Wu YC, Huang CY (2009b) VEGFA upregulates FLJ10540 and modulates migration and invasion of lung cancer via PI3K/AKT pathway. PLoS ONE [Electronic Resource] 4:e5052

Chen CH, Lu PJ, Chen YC, Fu SL, Wu KJ, Tsou AP, Lee YC, Lin TC, Hsu SL, Lin WJ, Huang CY, Chou CK, Lee YCG, Lin TCE, Huang CYF (2007) FLJ10540-elicited cell transformation is through the activation of PI3-kinase/AKT pathway. Oncogene 26:4272-4283

Chopra P, Sethi G, Dastidar SG, Ray A (2010) Polo-like kinase inhibitors: an emerging opportunity for cancer therapeutics. Expert Opinion on Investigational Drugs 19:27-43

Dai C, Gu W (2010) p53 post-translational modification: deregulated in tumorigenesis. Trends in Molecular Medicine 16:528-536

Doxsey S, McCollum D, Theurkauf W (2005) Centrosomes in cellular regulation. Annual Review of Cell & Developmental Biology 21:411-434

Doxsey SJ (2005) Molecular links between centrosome and midbody. Molecular Cell 20:170-172

Doxsey SJ, Stein P, Evans L, Calarco PD, Kirschner M (1994) Pericentrin, a highly conserved centrosome protein involved in microtubule organization.[see comment]. Cell 76:639-650

Dym M, Fawcett DW (1971) Further observations on the numbers of spermatogonia, spermatocytes, and spermatids connected by intercellular bridges in the mammalian testis. Biology of Reproduction 4:195-215

Eggert US, Mitchison TJ, Field CM (2006) Animal cytokinesis: from parts list to mechanisms. Annual Review of Biochemistry 75:543-566

el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW, Vogelstein B (1993) WAF1, a potential mediator of p53 tumor suppression. Cell 75:817-825

Fabbro M, Zhou BB, Takahashi M, Sarcevic B, Lal P, Graham ME, Gabrielli BG, Robinson PJ, Nigg EA, Ono Y, Khanna KK (2005) Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis. Developmental Cell 9:477-488

Farnebo M, Bykov VJ, Wiman KG (2010) The p53 tumor suppressor: a master regulator of diverse cellular processes and therapeutic target in cancer. Biochemical & Biophysical Research Communications 396:85-89

Fawcett DW, Ito S, Slautterback D (1959) The occurrence of intercellular bridges in groups of cells exhibiting synchronous differentiation. Journal of Biophysical & Biochemical Cytology 5:453-460

Fu Z, Malureanu L, Huang J, Wang W, Li H, van Deursen JM, Tindall DJ, Chen J (2008) Plk1-dependent phosphorylation of FoxM1 regulates a transcriptional programme required for mitotic progression. Nature Cell Biology 10:1076-1082

Gemenetzidis E, Bose A, Riaz AM, Chaplin T, Young BD, Ali M, Sugden D, Thurlow JK, Cheong SC, Teo SH, Wan H, Waseem A, Parkinson EK, Fortune F, Teh MT (2009) FOXM1 upregulation is an early event in human squamous cell carcinoma and it is enhanced by nicotine during malignant transformation. PLoS ONE [Electronic Resource] 4:e4849

Glotzer M (2005) The molecular requirements for cytokinesis. Science 307:1735-1739

Glotzer M (2001) Animal cell cytokinesis. Annual Review of Cell & Developmental Biology 17:351-386

Greenbaum MP, Iwamori N, Agno JE, Matzuk MM (2009) Mouse TEX14 is required for embryonic germ cell intercellular bridges but not female fertility. Biology of Reproduction 80:449-457

Greenbaum MP, Ma L, Matzuk MM (2007) Conversion of midbodies into germ cell intercellular bridges. Developmental Biology 305:389-396

Greenbaum MP, Yan W, Wu MH, Lin YN, Agno JE, Sharma M, Braun RE, Rajkovic A, Matzuk MM (2006) TEX14 is essential for intercellular bridges and fertility in male mice. Proceedings of the National Academy of Sciences of the United States of America 103:4982-4987

Gromley A, Yeaman C, Rosa J, Redick S, Chen CT, Mirabelle S, Guha M, Sillibourne J, Doxsey SJ (2005) Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission. Cell 123:75-87

Guo GQ, Zheng GC (2004) Hypotheses for the functions of intercellular bridges in male germ cell development and its cellular mechanisms. Journal of Theoretical Biology 229:139-146

Hasegawa S, Furukawa Y, Li M, Satoh S, Kato T, Watanabe T, Katagiri T, Tsunoda T, Yamaoka Y, Nakamura Y (2002) Genome-wide analysis of gene expression in intestinal-type gastric cancers using a complementary DNA microarray representing 23,040 genes. Cancer Research 62:7012-7017

Hermo L, Pelletier RM, Cyr DG, Smith CE (2010) Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 1: background to spermatogenesis, spermatogonia, and spermatocytes. Microscopy Research & Technique 73:241-278

Hess RA, Renato de Franca L (2008) Spermatogenesis and cycle of the seminiferous epithelium. Advances in Experimental Medicine & Biology 636:1-15

Hollstein M, Sidransky D, Vogelstein B, Harris CC (1991) p53 mutations in human cancers. Science 253:49-53

Inoda S, Hirohashi Y, Torigoe T, Morita R, Takahashi A, Asanuma H, Nakatsugawa M, Nishizawa S, Tamura Y, Tsuruma T, Terui T, Kondo T, Ishitani K, Hasegawa T, Hirata K, Sato N (2011a) Cytotoxic T lymphocytes efficiently recognize human colon cancer stem-like cells. The American Journal of Pathology 178:1805-1813

Inoda S, Hirohashi Y, Torigoe T, Nakatsugawa M, Kiriyama K, Nakazawa E, Harada K, Takasu H, Tamura Y, Kamiguchi K, Asanuma H, Tsuruma T, Terui T, Ishitani K, Ohmura T, Wang Q, Greene MI, Hasegawa T, Hirata K, Sato N (2009) Cep55/c10orf3, a tumor antigen derived from a centrosome residing protein in breast carcinoma. Journal of Immunotherapy 32:474-485

Inoda S, Morita R, Hirohashi Y, Torigoe T, Asanuma H, Nakazawa E, Nakatsugawa M, Tamura Y, Kamiguchi K, Tsuruma T, Terui T, Ishitani K, Hashino S, Wang Q, Greene MI, Hasegawa T, Hirata K, Asaka M, Sato N (2011b) The feasibility of Cep55/c10orf3 derived peptide vaccine therapy for colorectal carcinoma. Experimental & Molecular Pathology 90:55-60

Iwamori T, Iwamori N, Ma L, Edson MA, Greenbaum MP, Matzuk MM (2010) TEX14 interacts with CEP55 to block cell abscission. Molecular & Cellular Biology 30:2280-2292

Johnson KJ, Zecevic A, Kwon EJ (2004) Protocadherin alpha3 acts at sites distinct from classic cadherins in rat testis and sperm. Biology of Reproduction 70:303-312

Kato A, Nagata Y, Todokoro K (2004a) Delta-tubulin is a component of intercellular bridges and both the early and mature perinuclear rings during spermatogenesis. Developmental Biology 269:196-205

Kato A, Nagata Y, Todokoro K, Kato A, Nagata Y, Todokoro K (2004b) Delta-tubulin is a component of intercellular bridges and both the early and mature perinuclear rings during spermatogenesis. Developmental Biology 269:196-205

Kho PS, Wang Z, Zhuang L, Li Y, Chew JL, Ng HH, Liu ET, Yu Q (2004) p53-regulated transcriptional program associated with genotoxic stress-induced apoptosis. Journal of Biological Chemistry 279:21183-21192

Kikuchi T, Daigo Y, Katagiri T, Tsunoda T, Okada K, Kakiuchi S, Zembutsu H, Furukawa Y, Kawamura M, Kobayashi K, Imai K, Nakamura Y (2003) Expression profiles of non-small cell lung cancers on cDNA microarrays: identification of genes for prediction of lymph-node metastasis and sensitivity to anti-cancer drugs. Oncogene 22:2192-2205

Kneisel L, Strebhardt K, Bernd A, Wolter M, Binder A, Kaufmann R (2002) Expression of polo-like kinase (PLK1) in thin melanomas: a novel marker of metastatic disease. Journal of Cutaneous Pathology 29:354-358

Krawetz SA, De Rooij DG, Hedger MP (2009) Molecular aspects of male fertility. International Workshop on Molecular Andrology. EMBO Reports 10:1087-1092

Leblond CP, Clermont Y (1952) Definition of the stages of the cycle of the seminiferous epithelium in the rat. Annals of the New York Academy of Sciences 55:548-573

Lee HH, Elia N, Ghirlando R, Lippincott-Schwartz J, Hurley JH (2008) Midbody targeting of the ESCRT machinery by a noncanonical coiled coil in CEP55. Science 322:576-580

Lenart P, Petronczki M, Steegmaier M, Di Fiore B, Lipp JJ, Hoffmann M, Rettig WJ, Kraut N, Peters JM, Lenart P, Petronczki M, Steegmaier M, Di Fiore B, Lipp JJ, Hoffmann M, Rettig WJ, Kraut N, Peters J-M (2007) The small-molecule inhibitor BI 2536 reveals novel insights into mitotic roles of polo-like kinase 1. Current Biology 17:304-315

Levine AJ (1997) p53, the cellular gatekeeper for growth and division. Cell 88:323-331

Ma XJ, Salunga R, Tuggle JT, Gaudet J, Enright E, McQuary P, Payette T, Pistone M, Stecker K, Zhang BM, Zhou YX, Varnholt H, Smith B, Gadd M, Chatfield E, Kessler J, Baer TM, Erlander MG, Sgroi DC (2003) Gene expression profiles of human breast cancer progression. Proceedings of the National Academy of Sciences of the United States of America 100:5974-5979

Manandhar G, Schatten H, Sutovsky P (2005) Centrosome reduction during gametogenesis and its significance. Biology of Reproduction 72:2-13

Manandhar G, Simerly C, Salisbury JL, Schatten G (1999) Centriole and centrin degeneration during mouse spermiogenesis. Cell Motility & the Cytoskeleton 43:137-144

Martinez-Garay I, Rustom A, Gerdes HH, Kutsche K (2006) The novel centrosomal associated protein CEP55 is present in the spindle midzone and the midbody. Genomics 87:243-253

McKenzie L, King S, Marcar L, Nicol S, Dias SS, Schumm K, Robertson P, Bourdon JC, Perkins N, Fuller-Pace F, Meek DW (2010) p53-dependent repression of polo-like kinase-1 (PLK1). Cell Cycle 9:4200-4212

Minestrini G, Mathe E, Glover DM (2002) Domains of the Pavarotti kinesin-like protein that direct its subcellular distribution: effects of mislocalisation on the tubulin and actin cytoskeleton during Drosophila oogenesis. Journal of Cell Science 115:725-736

Mishima M, Kaitna S, Glotzer M (2002a) Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity.[see comment]. Developmental Cell 2:41-54

Mishima M, Kaitna S, Glotzer M, Mishima M, Kaitna S, Glotzer M (2002b) Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity.[see comment]. Developmental Cell 2:41-54

Montoya M (2007) An ESCRT for daughters. Nature Structural & Molecular Biology 14:579

Morita E, Sandrin V, Chung HY, Morham SG, Gygi SP, Rodesch CK, Sundquist WI (2007) Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis. EMBO Journal 26:4215-4227

Muller PA, Vousden KH, Norman JC (2011) p53 and its mutants in tumor cell migration and invasion. Journal of Cell Biology 192:209-218

Mullins JM, Biesele JJ (1977) Terminal phase of cytokinesis in D-98s cells. Journal of Cell Biology 73:672-684

Mullins JM, Biesele JJ (1973) Cytokinetic activities in a human cell line: the midbody and intercellular bridge. Tissue & Cell 5:47-61

Mundt KE, Golsteyn RM, Lane HA, Nigg EA (1997) On the regulation and function of human polo-like kinase 1 (PLK1): effects of overexpression on cell cycle progression. Biochemical & Biophysical Research Communications 239:377-385

Myatt SS, Lam EW (2007) The emerging roles of forkhead box (Fox) proteins in cancer. Nature Reviews Cancer 7:847-859

Nakamura T, Furukawa Y, Nakagawa H, Tsunoda T, Ohigashi H, Murata K, Ishikawa O, Ohgaki K, Kashimura N, Miyamoto M, Hirano S, Kondo S, Katoh H, Nakamura Y, Katagiri T (2004) Genome-wide cDNA microarray analysis of gene expression profiles in pancreatic cancers using populations of tumor cells and normal ductal epithelial cells selected for purity by laser microdissection. Oncogene 23:2385-2400

Oakberg EF (1956) A description of spermiogenesis in the mouse and its use in analysis of the cycle of the seminiferous epithelium and germ cell renewal. American Journal of Anatomy 99:391-413

Okabe H, Satoh S, Kato T, Kitahara O, Yanagawa R, Yamaoka Y, Tsunoda T, Furukawa Y, Nakamura Y (2001) Genome-wide analysis of gene expression in human hepatocellular carcinomas using cDNA microarray: identification of genes involved in viral carcinogenesis and tumor progression. Cancer Research 61:2129-2137

Pandit B, Halasi M, Gartel AL (2009) p53 negatively regulates expression of FoxM1. Cell Cycle 8:3425-3427

Petronczki M, Lenart P, Peters JM (2008) Polo on the Rise-from Mitotic Entry to Cytokinesis with Plk1. Developmental Cell 14:646-659

Plyte S, Musacchio A (2007) PLK1 inhibitors: setting the mitotic death trap. Current Biology 17:R280-283

Pohl C, Jentsch S, Pohl C, Jentsch S (2008) Final stages of cytokinesis and midbody ring formation are controlled by BRUCE. Cell 132:832-845

Prives C, Hall PA (1999) The p53 pathway. Journal of Pathology 187:112-126

Rattner JB (1992) Mapping the mammalian intercellular bridge. Cell Motility & the Cytoskeleton 23:231-235

Robinson DN, Cant K, Cooley L (1994) Morphogenesis of Drosophila ovarian ring canals. Development 120:2015-2025

Robinson DN, Cooley L (1996) Stable intercellular bridges in development: the cytoskeleton lining the tunnel. Trends Cell Biol 6:474-479

Rozan LM, El-Deiry WS (2007) p53 downstream target genes and tumor suppression: a classical view in evolution. Cell Death & Differentiation 14:3-9

Russell LD, Vogl AW, Weber JE (1987) Actin localization in male germ cell intercellular bridges in the rat and ground squirrel and disruption of bridges by cytochalasin D. American Journal of Anatomy 180:25-40

Sachdeva M, Zhu S, Wu F, Wu H, Walia V, Kumar S, Elble R, Watabe K, Mo YY (2009) p53 represses c-Myc through induction of the tumor suppressor miR-145. Proceedings of the National Academy of Sciences of the United States of America 106:3207-3212

Saint R, Somers WG (2003) Animal cell division: a fellowship of the double ring? Journal of Cell Science 116:4277-4281

Saint R, Somers WG, Saint R, Somers WG (2003) Animal cell division: a fellowship of the double ring? Journal of Cell Science 116:4277-4281

Sakai M, Shimokawa T, Kobayashi T, Matsushima S, Yamada Y, Nakamura Y, Furukawa Y (2006) Elevated expression of C10orf3 (chromosome 10 open reading frame 3) is involved in the growth of human colon tumor. Oncogene 25:480-486

Schoffski P, Schoffski P (2009) Polo-like kinase (PLK) inhibitors in preclinical and early clinical development in oncology. Oncologist 14:559-570

Sionov RV, Haupt Y (1999) The cellular response to p53: the decision between life and death. Oncogene 18:6145-6157

Somers WG, Saint R (2003) A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis. Developmental Cell 4:29-39

Somers WG, Saint R, Somers WG, Saint R (2003) A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis. Developmental Cell 4:29-39

Soussi T, Beroud C (2001) Assessing TP53 status in human tumours to evaluate clinical outcome. Nature Reviews Cancer 1:233-240

Steegmaier M, Hoffmann M, Baum A, Lenart P, Petronczki M, Krssak M, Gurtler U, Garin-Chesa P, Lieb S, Quant J, Grauert M, Adolf GR, Kraut N, Peters JM, Rettig WJ (2007) BI 2536, a potent and selective inhibitor of polo-like kinase 1, inhibits tumor growth in vivo. Current Biology 17:316-322

Sur S, Pagliarini R, Bunz F, Rago C, Diaz LA, Jr., Kinzler KW, Vogelstein B, Papadopoulos N (2009) A panel of isogenic human cancer cells suggests a therapeutic approach for cancers with inactivated p53. Proceedings of the National Academy of Sciences of the United States of America 106:3964-3969

Takaki T, Trenz K, Costanzo V, Petronczki M (2008) Polo-like kinase 1 reaches beyond mitosis--cytokinesis, DNA damage response, and development. Current Opinion in Cell Biology 20:650-660

Terasima T, Tolmach LJ (1963) Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Experimental Cell Research 30:344-362

Tobey RA, Anderson EC, Petersen DF (1967) Properties of mitotic cells prepared by mechanically shaking monolayer cultures of Chinese hamster cells. J Cell Physiol 70:63-68

Tres LL, Rivkin E, Kierszenbaum AL (1996) Sak 57, an intermediate filament keratin present in intercellular bridges of rat primary spermatocytes. Molecular Reproduction & Development 45:93-105

van de Weerdt BC, Medema RH (2006) Polo-like kinases: a team in control of the division. Cell Cycle 5:853-864

van der Horst A, Simmons J, Khanna KK (2009) Cep55 stabilization is required for normal execution of cytokinesis. Cell Cycle 8:3742-3749

van Vugt MA, Medema RH (2005) Getting in and out of mitosis with Polo-like kinase-1. Oncogene 24:2844-2859

Ventela S, Toppari J, Parvinen M (2003) Intercellular organelle traffic through cytoplasmic bridges in early spermatids of the rat: mechanisms of haploid gene product sharing. Molecular Biology of the Cell 14:2768-2780

Vousden KH, Lu X (2002) Live or let die: the cell's response to p53. Nature Reviews Cancer 2:594-604

Vousden KH, Prives C, Vousden KH, Prives C (2009) Blinded by the Light: The Growing Complexity of p53. Cell 137:413-431

Wasch R, Hasskarl J, Schnerch D, Lubbert M (2010) BI_2536--targeting the mitotic kinase Polo-like kinase 1 (Plk1). Recent Results in Cancer Research 184:215-218

Weber JE, Russell LD (1987) A study of intercellular bridges during spermatogenesis in the rat. American Journal of Anatomy 180:1-24

Weichert W, Denkert C, Schmidt M, Gekeler V, Wolf G, Kobel M, Dietel M, Hauptmann S (2004a) Polo-like kinase isoform expression is a prognostic factor in ovarian carcinoma. British Journal of Cancer 90:815-821

Weichert W, Schmidt M, Gekeler V, Denkert C, Stephan C, Jung K, Loening S, Dietel M, Kristiansen G (2004b) Polo-like kinase 1 is overexpressed in prostate cancer and linked to higher tumor grades. Prostate 60:240-245

Yu J, Zhang L, Yu J, Zhang L (2003) No PUMA, no death: implications for p53-dependent apoptosis. Cancer Cell 4:248-249

Zhao WM, Seki A, Fang G (2006) Cep55, a microtubule-bundling protein, associates with centralspindlin to control the midbody integrity and cell abscission during cytokinesis. Molecular Biology of the Cell 17:3881-3896

連結至畢業學校之論文網頁點我開啟連結
註: 此連結為研究生畢業學校所提供,不一定有電子全文可供下載,若連結有誤,請點選上方之〝勘誤回報〞功能,我們會盡快修正,謝謝!
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔