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研究生:尹柏力
研究生(外文):Boris Ivanic
論文名稱:於囓齒類動物模型探討電針保護古柯鹼引發癲癇之作用:行為及組織免疫化學之研究
論文名稱(外文):Electroacupuncture protects cocaine-induced seizure in a murine model: a behavior and immunohistochemistry study
指導教授:陳易宏陳易宏引用關係
學位類別:碩士
校院名稱:中國醫藥大學
系所名稱:國際針灸碩士學位學程
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:英文
論文頁數:68
中文關鍵詞:電針古柯鹼c-fos古柯鹼引起之相關症狀
外文關鍵詞:electroacupuncturecocainec-foscocaine-induced seizure
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本研究以免疫組織化學方法來探討電針(electroacupuncture, EA)對古柯鹼(cocaine,75 mg/kg)在小鼠腦中造成c-fos表現之影響。在固定動物姿勢與不施以麻醉的狀況下,將實驗分為三組:(1)控制組:不施行電針;(2)電針組:於動 物GV14(大椎)與GV20(百會)施行電針(50 Hz ; 1mA)和(3)假電針組:電針動物不具穴位之尾巴(非穴位)。電針完一分鐘後,於腹腔注射古柯鹼(75 mg/kg)。古柯鹼注射兩小時後,犧牲所有實驗動物並利用免疫組織化學的方法觀察其腦中c-fos的表現狀態。

實驗結果發現,在電針GV14與GV20的電針組可降低古柯鹼對於小鼠所引起的毒性症狀,並減低下丘腦外側區和丘腦的腦室壁核中由古柯鹼所引起的c-fos表現。


Abstract

We tested the effects of electroacupuncture (EA) on seizures induced by single administration of cocaine (75 mg/kg) in mice and followed by an imunohistochemistry study to determine the c-fos expression in the brain. Three groups of animals were included, i.e. (1) control group: animals were strained without EA, (2) EA treatment group: animals were strained and received EA (50 Hz; 1mA) for 15 min at GV14 (Dazhui) and GV20 (Baihui) without anesthesia before cocaine injection and (3) sham acupuncture group, where EA was performed on the tail (non-acupoints) of the animal. EA treatments were followed 1 min afterwards by an intraperitoneal injection of cocaine (75 mg/kg). At two hours after cocaine injection, all groups were sacrificed and the c-fos express in the brain was determined by an immunohistochemistry method. In addition, we also examined the c-fos expression after applicationof EA to GV14 and GV20, without cocaine injection.
We found that EA pretreatment at GV14 and GV20 attenuated the severity of cocaine-induced seizure in EA group and decreased cocaine-induced c-fos expression in paraventrical nucleus of thalamus and lateral hypothalamic area.




Abstract iv
I. Introduction and literature preview 1
1. Introduction 1
2. Prevalence of cocaine usage 1
3. Cocaine and the brain 2
4. Effect of cocaine on dopamine in the brain 3
5. Seizures associated with cocaine 4
6. Incidence of cocaine related seizures 5
7. Cocaine associated seizures mechanism 5
8. Role of serotonin in cocaine induced seizures 6
9. Treatments of cocaine-induced seizures 8
10. Cocaine and c-fos expression 8
11. Acupuncture introduction 10
12. Acupuncture treatment of drug addiction 10
13. Acupuncture mechanism in the treatment of drug addiction 11
14. Acupuncture and seizures 13
15. Reason why I study cocaine-related seizures and acupuncture. 15
II. Study design and methods 16
1. Laboratory animals 16
2. EA group 16
3. Control group 18
4. Sham acupuncture group 19
5. Stress related groups 19
6. Drugs and chemicals 20
7. Behavioral study 21
a) To determine how EA decreases cocaine-induced acute seizures and death in mice 21
b) To determine how EA influence onset of cocaine-induced acute seizures. 25
8. Immunohistochemistry procedure 27
9. Fos immunoreactivity analysis 28
10. Fos immunoreactivity analysis related to stress response from immobilization and EA
performed on the animals. 28
11. Data analysis 31
III. Results 32
1. Behavioral study 32
a) EA decreases severity of cocaine-induced acute seizures in mice 32
b) EA not prolong the onset of cocaine-induced acute seizures 34
1. Immunohistochemistry study 36
a) Dentate gyrus 36
b) Caudoputamen 41
c) Amygdala 45
d) Paraventrical nucleus of thalamus 49
e) Lateral hypothalamic area 53
2. Stress induced c-fos expression 57
IV. Discussion 60
V. Conclusion 63
VI. References 64



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