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研究生:顧洽任
研究生(外文):CHA-REN GU
論文名稱:牛初乳水解物之普拉斯丁產物在體外之免疫調節及細胞活性
論文名稱(外文):In Vitro Immunomodulatory and Cellular Activities of the Plastein Reaction Products of Bovine Colostrum Hydrolysates
指導教授:張基郁張基郁引用關係
指導教授(外文):CHI-YUE CHANG
口試委員:吳淑姿曾慶瀛
口試委員(外文):Shwu-Tzy WuChin-Yin Tseng
口試日期:2012-06-26
學位類別:碩士
校院名稱:大葉大學
系所名稱:生物產業科技學系
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:100
中文關鍵詞:牛初乳普拉斯丁白血病細胞株U937細胞激素Flavourzyme
外文關鍵詞:Bovine colostrums、Plasteins、Leukemic U937 cells、Cytokines、Flavourzyme
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本研究用母牛分娩後第2天分泌之初乳,經Flavourzyme水解後,所得之初乳水解物,在pH 5和9條件下,加入proline以Flavourzyme進行普拉斯丁反應4、8及12小時後之普拉斯丁產物,與人類單核球細胞培養1及3天後之條件培養液(MNC-CM),再進行U937細胞生長之抑制及免疫調節之活性試驗。結果顯示,在pH 9反應8小時之普拉斯丁產物(Pp-9-8),在濃度800μg/mL下,其對U937細胞生長之抑制效果及細胞激素之分泌量皆具有顯著之效果。另外以3種胺基酸(Tyrosine, Leucine, Glycine)在pH 9和反應8小時之條件下進行普拉斯丁反應,所得之產物(Tp-9-8, Lp-9-8, Gp-9-8)與Pp-9-8進行相同試驗之比較。結果顯示,Pp-9-8與Lp-9-8對U937細胞生長之抑制皆具有顯著之效果,且可顯著刺激細胞激素之分泌。本研究亦對Pp-9-8及Lp-9-8進行模擬腸胃道消化試驗,探討經由腸胃道酵素作用後,對U937細胞生長之抑制及免疫調節之功效。結果顯示,經模擬腸胃道之普拉斯丁產物,對U937細胞生長之抑制及細胞激素之分泌量均有降低之趨勢。
In this research the skimmed milk isolated from colostrums collected on the 2nd day postpartum were hydrolyzed by Flavourzyme. The skimmed milk hydrolysates were subjected into a plastein reaction with Flavourzyme under pH 5 and 9 for 4, 8 and 12 hours, respectively. This research investigates the effects of different plastin products on human mononuclear cell (MNC) growth, on the secretion of cytokines (IL-1β, IFN-γ and TNF-α) as well as nitric oxide, and on the growth inhibition of human leukemic U937 cells.
The results showed that the products of plastein reaction with Flavourzyme under pH 9 for 8 hours (Pp-9-8) (at 800 μg/mL) had significant effects on the inhibition of U937 cell growth and cytokine secretion. Additionally, Tp-9-8, Lp-9-8 and Gp-9-8 from the plastein reactions with three amino acids (Tyrosine, Leucine and Glycine) under pH 9 for 8 hours were compared with Pp-9-8. It was found that both Pp-9-8 and Lp-9-8 had significant effects on the inhibition of U937 cell growth, and could considerably stimulate the secretion of cytokines. This research also investigated the simulated digestion in the gastrointestinal tract for Pp-9-8 and Lp-9-8 and the effects of the enzymes in the gastrointestinal tracts on the growth inhibition and immunoregulation of U937 cells. Results showed that the plastein products after the simulated digestion in the gastrointestinal tracts had a tendency to decrease both the inhibition of U937 cell growth and the amount of cytokine secreted.

目錄

封面內頁
簽名頁
中文摘要iii
英文摘要iv
誌謝v
目錄vii
圖目錄xi
表目錄xiii
符號說明xiv

1. 緒論1
2. 文獻回顧2
2.1 牛初乳2
2.1.1 牛乳蛋白之水解物應用4
2.2 牛初乳中的生物活性成分4
2.2.1 乳鐵蛋白4
2.2.2 生長因子5
2.2.3 細胞激素8
2.3 酵素水解11
2.3.1 蛋白質水解物之應用11
2.3.2 酵素基質比11

2.3.3 溫度與pH12
2.3.4 普拉斯丁反應及特性12
2.4 人類白血病細胞株U93713
3. 材料與方法15
3.1 實驗材料15
3.1.1 材料15
3.1.2 藥品16
3.1.3 重要儀器16
3.2 實驗方法與分析18
3.2.1 實驗流程18
3.2.2 基本組成成分分析19
3.2.3 脫脂牛初乳之水解與普拉斯丁反應21
3.2.4 普拉斯丁經由間接模式對U937細胞抑制用實驗22
3.2.5 統計分析27
4. 實驗結果28
4.1 牛初乳之一般組成分28
4.2 加入Proline之普拉斯丁產物之免疫調節及細胞活性30
4.2.1 加入Proline普拉斯丁產物經由間接抑制U937細胞生長試驗30
4.2.2 加入Proline之普拉斯丁之MNC條件培養液中IL-1β之表現量33
4.2.3 加入Proline之普拉斯丁之MNC條件培養液中IFN-γ之表現量36
4.2.4 加入Proline之普拉斯丁之MNC條件培養液中TNF-α之表現量39
4.2.5 加入Proline之普拉斯丁產物之MNC條件培養液中一氧化氮(NO)之含量42
4.2.6 加入Proline之普拉斯丁產物刺激人類單核球細胞之細胞生長率44
4.3 加入不同胺基酸之普拉斯丁產物之免疫調節及細胞活性47
4.3.1 加入不同胺基酸之普拉斯丁產物對U937細胞生長之間接抑制試驗47
4.3.2 加入不同胺基酸之普拉斯丁產物之條件培養液對IL-1β表現量之影響49
4.3.3 加入不同胺基酸之普拉斯丁產物之條件培養液對IFN-γ表現量之影響51
4.3.4 加入不同胺基酸之普拉斯丁產物之條件培養液對TNF-α表現量之影響53
4.3.5 加入不同胺基酸之普拉斯丁產物之條件培養液中一氧化氮(NO)之含量55
4.3.6 加入不同胺基酸之普拉斯丁產物之條件培養液刺激人類單核球細胞1及3天後之細胞生長57
4.4 加入Proline及Leucine之普拉斯丁產物經模擬腸胃道後之免疫調節及細胞活性60
4.4.1 經模擬腸胃道之普拉斯丁產物對U937細胞生長之間接抑制試驗60
4.4.2 經模擬腸胃道試驗後之普拉斯丁產物對單核球細胞生長率及細胞激素與一氧化氮表現量之影響62
5. 討論69
5.1普拉斯丁產物對U937細胞生長之間接抑制69
5.2普拉斯丁產物對人類單核球細胞生長之效果與細胞激素及NO之表現量70
5.3經模擬腸胃道試驗之普拉斯丁產物對U937細胞生長之抑制及免疫調節之活性72
6. 結論74
參考文獻76

圖目錄
圖3.1本研究實驗流程圖18
圖4.1普拉斯丁產物刺激人類單核細胞條件培養液1天對U937細胞抑制率之比較31
圖4.2普拉斯丁產物刺激人類單核細胞條件培養液3天對U937細胞抑制率之比較32
圖4.3普拉斯丁產物刺激人類單核細胞條件培養液1天所產生IL-1β之表現量之比較34
圖4.4普拉斯丁產物刺激人類單核細胞條件培養液3天所產生IL-1β之表現量之比較35
圖4.5普拉斯丁產物刺激人類單核細胞條件培養液1天產生IFN-γ之表現量之比較37
圖4.6普拉斯丁產物刺激人類單核細胞條件培養液3天產生IFN-γ之表現量之比較38
圖4.7普拉斯丁產物刺激人類單核細胞條件培養液1天所產生TNF-α含量之比較40
圖4.8普拉斯丁產物刺激人類單核細胞條件培養液3天所產生TNF-α含量之比較41
圖4.9普拉斯丁產物(Pp-9-8, Tp-9-8, Lp-9-8, Gp-9-8;800 μg/mL)刺激人類單核細胞條件培養液1及3天後對U937細胞之生長抑制率48
圖4.10普拉斯丁產物(Pp-9-8, Tp-9-8, Lp-9-8, Gp-9-8 ; 800μg/mL)刺激人類單核細胞條件培養液1及3天後之IL-1β含量之比較50
圖4.11普拉斯丁產物(Pp-9-8, Tp-9-8, Lp-9-8, Gp-9-8 ; 800μg/mL)刺激人類單核細胞條件培養液1及3天後之IFN-γ含量之比較52
圖4.12普拉斯丁產物(Pp-9-8, Tp-98, Lp-98, Gp-9-8 ; 800μg/mL)刺激人類單核細胞條件培養液1及3天後之TNF-α含量之比較54
圖4.13普拉斯丁產物經模擬腸胃道後(Lp-9-8,Pp-9-8-GS)刺激人類單核細胞條件培養液1及3天對U937細胞生長之抑制率61
圖4.14普拉斯丁產物經模擬腸胃道試驗後(Lp-9-8-GS與Pp-9-8-GS)刺激人類單核細胞培養液1及3天產生IL-1β含量之比較64
圖4.15普拉斯丁產物經模擬腸胃道試驗後(Lp-9-8-GS與Pp-9-8-GS)刺激人類單核細胞培養液1及3天產生IFN-γ含量之比較65
圖4.16普拉斯丁產物經模擬腸胃道試驗後(Lp-9-8-GS與Pp-9-8-GS)刺激人類單核細胞培養液1及3天產生TNF-α含量之比較66

表目錄
表2.1牛初乳與常乳組成成分比較3
表2.2牛初乳於產後不同時間之TGF-β2 、IGF-1 及GH之濃度7
表4.1母牛分娩後第2天之初乳之一般組成份29
表4.2加入proline之普拉斯丁產物刺激MNC條件培養液1及3天之一氧化氮(NO)含量43
表4.3加入proline普拉斯丁產物刺激人類單核球細胞1及3天之細胞生長率45
表4.4普拉斯丁產物(Pp-9-8, Tp-9-8, Lp-9-8, Gp-9-8;800μg/mL)刺激MNC條件培養液1及3天之一氧化氮(NO)含量56
表4.5普拉斯丁產物(Pp-9-8, Tp-9-8, Lp-9-8, Gp-9-8;800μg/mL)刺激人類單核球細胞1及3天之細胞生長率58
表4.6普拉斯丁產物經模擬腸胃道試驗後(Lp-9-8-GS與Pp-9-8-GS)刺激MNC條件培養液1及3天之一氧化氮(NO)含量67
表4.7普拉斯丁產物經模擬腸胃道試驗後刺激人類單核球細胞1及3天之細胞生長率68

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