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研究生:陳奕齊
研究生(外文):Chen, Yi-Chi
論文名稱:黃梔子萃出物對血糖及胰島素敏感性影響之探討
論文名稱(外文):Effect of Gardenia jasmindides Ellis Extract on Plasma Glucose and Insulin Sensitivity
指導教授:李世傑李世傑引用關係張世良張世良引用關係張耀南張耀南引用關係
指導教授(外文):Lee, Shih-ChiehChang, Shih-LiangChang, Yao-Nan
口試委員:李世傑張世良張耀南李泰林劉承慈
口試委員(外文):Lee, Shih-ChiehChang, Shih-LiangChang, Yao-NanLee, Tai-LinLiu,Cheng-Tzu
口試日期:2012-07-17
學位類別:碩士
校院名稱:大葉大學
系所名稱:生物產業科技學系
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:63
中文關鍵詞:糖尿病血糖胰島素游離脂肪酸胰島素阻抗黃梔子
外文關鍵詞:diabetesglucoseinsulinfree fatty acidsGardenia jasmindides Ellisinsulin sensitizers
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糖尿病是一種因胰島素分泌或胰島素作用缺陷而影響糖、脂肪及蛋白質的代謝紊亂所照成的代謝疾病。目前世界衛生組織衛生組織將糖尿病分為三類:第1型糖尿病、第2型糖尿病、妊娠型糖尿病,其中第2型糖尿病患者占90%。胰島素阻抗是導致第二型糖尿病的主要因素。胰島素阻抗是指在正常的胰島素濃度不能與脂肪、肌肉和肝臟等組織產生正常胰島素的反應,也就是組織對於胰島素的敏感性降低。
  黃梔子在過去文獻已提出具有降血糖、降胰島素及降血脂等改善糖尿病活性,但其機制尚未完全清楚,本研究首先利用正常大鼠餵食不同濃度黃梔子萃出物以尋找降血糖的最佳劑量,並運用在經由類固醇誘發的胰島素阻抗大鼠上,觀察是否也具有降血糖的效果。接著在類固醇誘發的胰島素阻抗大鼠上,評估其改善胰島素敏感性的效果,最後檢測血中游離脂肪酸,探討黃梔子萃出物藉由影響血中游離脂肪酸來達到改善胰島素敏感性的效果。
  結果顯示:黃梔子萃出物在正常大鼠表現出降血糖的效果,且在餵食濃度200 mg/kg時有較佳的降糖率。且在類固醇誘發的胰島素阻抗大鼠也有顯著降低血糖的效果。而在胰島素增敏評估中,發現黃梔子萃出物具有增加胰島素敏感度的表現,但血中游離脂肪酸的改變未達統計顯著差異,可能黃梔子萃出物增加胰島素敏感度的作用,不是藉由降低血中游離脂肪酸達到效果,但可能因為餵食的時間較短,故無法顯現效果,必須進一步的研究,才能明確表示黃梔子對於游離脂肪酸的影響。
  結論:本研究發現黃梔子萃出物在餵食濃度200 mg/kg時,具有顯著的降糖效果並且能改善類固醇誘發胰島素阻抗大鼠的高血糖症狀,以及增加胰島素敏感度,藉此能提供未來發展黃梔子做為胰島素增敏劑的方向。

Diabetes is a metabolic disorder, the defects of insulin secretion or insulin action affect the sugar, fat and protein into the metabolic syndrome. World Health Organization WHO, diabetes is divided into three categories: type 1 diabetes (Type 1), type 2 diabetes (Type 2), pregnancy diabetes, which accounted for 90% of patients with type 2 diabetes. Insulin reisitance is a major factor leading to type II diabetes. Insulin reisitance is that normal insulin level cannot normally react with the tissue of adipose, muscle and liver.. etc. to produce normal reaction of insulin.  
  In the previous literatures of Gardenia Jasmindides Ellis have been proposed with lower blood glucose, insulin, and lipid to improve diabetes activity, but the mechanism is not yet fully established. In this study, normal rats were fed different concentrations of Gardenia Jasmindides Ellis extracts to find the best hypoglycemic dose, and used the steroid-induced insulin reISItant rats (SIIR) to observe whether exits the effect of lowering blood glucose or not. Then, on the steroid-induced insulin reISItant rats, for study the improving effect of insulin sensitivity, the plasma free fatty acids were assayed, to investigate the effect of gardenia extracts to improve insulin sensitivity by the impact of blood free fatty acids. .  
  The results showed: Gardenia extracts showed hypoglycemic effect of feeding dose 200 mg/kg with better hypoglycemic activity in normal rats. And steroid-induced insulin reISItant rats also have significantly hypoglycemic effect. In the assessment of insulin sensitivity, the gardenia extracts performed increasing insulin sensitivity, but the change plasma free fatty acids (FFA) cannot reach the statistical significant difference. The improving insulin sensitivity effect of gardenia extracts may be not through the changing plasma FFA. Also, the feeding time is shorter to can not show results, we must further study of gardenia extracts in plasma FFA. 
  Conclusion: This study found that the extracts of Gardenia Jasmindides Ellis in the dose 200mg/kg, has a significant hypoglycemic effect and can improve the insulin reISItaance on steroid-induced insulin reISItant rats. It has the potential to develop the gardenia as an insulin sensitizer in the future.

目錄

封面內頁
簽名頁
中文摘要 iii
英文摘要 v
誌謝 vii
目錄 viii
圖目錄 x
表目錄 xi
1. 前言 1
2. 研究目的 3
3. 文獻回顧 4
3.1 血糖恆定 4
3.2 胰島素的血糖調控 5
3.3 糖尿病與胰島素阻抗 7
3.4 中醫歷代對糖尿病的記載 11
3.5 黃梔子 14
4. 材料與方法 24
4.1 實驗前準備 24
4.2 實驗架構 26
4.3 實驗流程圖 27
4.4 檢驗方法 30
5. 結果 32
5.1黃梔子萃出物的降血糖最佳劑量 32
5.2黃梔子萃出物在SIIR的降血糖效果 35
5.3黃梔子萃出物的胰島素增敏效果評估 38
6. 討論 44
7. 結論 47
參考文獻 48
附錄 55

圖目錄
圖3-1 胰島素激活GLUT 4的傳遞路徑 6
圖3-2 游離脂肪酸對胰島素傳遞訊息的影響 10
圖3-3梔子苷元、梔子苷、梔子苷元-1-β-D- 龍膽二糖苷化學結構圖 17
圖3-4 苦藏紅花酸、梔子酮、梔子二醇化學結構圖 18
圖3-5 藏紅花素、藏紅花酸化學結構圖 19
圖3-6 齊敦果酸、熊果酸結構圖 20
圖3-7 黃梔子(台北市內雙溪森林藥用植物園) 23
圖4-1 黃梔子萃出物粉末(嘉年生化) 25
圖4-2 實驗架構 26
圖4-3 實驗一流程圖 27
圖4-4 實驗二流程圖 28
圖4-5 實驗三流程圖 29
圖5-1 黃梔子萃出物應用在Normal-rat的降糖百分率 34
圖5-2 Normal-rat及SIIR之血糖值比較 36
圖5-3 SIIR餵食saline及黃梔子萃出物200mg/kg後0、30、60分鐘後的血糖 37
圖5-4 黃梔子萃出物的胰島素功效評估:血糖值 39
圖5-5 黃梔子萃出物的胰島素功效評估:胰島素 40
圖5-6 黃梔子萃出物的胰島素功效評估: 胰島素敏感指標(ISI)41
圖5-7 黃梔子萃出物的胰島素功效評估: FFA 42

表目錄

表5-1 Normal-rat餵食saline及不同濃度的黃梔子萃出物後0、30、60分鐘後的血糖值 33
表5-2黃梔子萃出物應用SIIR大鼠的胰島素敏感度評估 43



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