臺灣博碩士論文加值系統

本研究之目的為發展一個可便於攜帶、使用簡單、成本低廉且準確性高的離心式三酸甘油酯檢測平台。本檢測平台利用微流體光碟分析儀所產生穩定性佳之離心力來驅動少量之樣本與試劑。在實驗中，將光碟旋轉系統與光學偵測系統整合並透過軟體介面進行操作，其檢測可以全血樣本進行並獲得結果。實驗最後將臨床檢測結果與台中榮民總醫院醫檢部所使用之全自動生化分析儀(HITACHI 7600)所得之測試結果進行比對後，得到良好的相關性。

This goal of this study is to develop point-of-care triglyceride assays on a centrifugal platform, which is portable, easy to use, low cost, while offering reasonable precision. The microfluidic disc analyzer is capable of actuating low volume samples and reagents through centrifugation. By integrating the disc spinning system and optical detection system through software interface, the testing results could be gathered from whole blood samples. Clinical samples were conducted by both the microfluidic disc analyzer and the instrument used in the hospital (Hitachi 7600), the test results showed good correlation and agreement.

致謝.............................................................................................................................. iii
摘要............................................................................................................................... iv
Abstract .......................................................................................................................... v
目錄............................................................................................................................... vi
圖目錄........................................................................................................................ viii
表目錄........................................................................................................................... ix
第一章緒論.................................................................................................................... 1
1.1血脂肪檢測.............................................................................................................. 1
1.2重點照護檢驗(Point-of-Care Testing, POCT) ........................................................ 2
1.3微流體光碟.............................................................................................................. 3
1.4 市售血脂肪檢測產品............................................................................................. 3
1.5研究動機.................................................................................................................. 7
第二章文獻回顧............................................................................................................ 8
2.1三酸甘油酯簡介...................................................................................................... 8
2.1.1血脂肪概述 ....................................................................................................... 8
2.1.2三酸甘油酯簡介(Triglyceride, TG) ................................................................. 9
2.1.3三酸甘油酯檢測 ............................................................................................. 10
2.2微流體功能............................................................................................................ 11
2.2.1 血漿傾注與定量 ............................................................................................ 12
2.2.1.1 使用疏水閥進行血漿傾注與定量 ......................................................... 12
2.2.1.2利用毛細管閥進行定量 .......................................................................... 12
2.2.1.3利用虹吸原理進行血漿傾注與定量 ...................................................... 13
2.2.2離心式混合器 ................................................................................................. 14
2.2.2.1利用壓縮氣體之氣動方式進行混合 ...................................................... 14
2.2.2.2利用磁珠來回震盪使流體混合 .............................................................. 14
2.3微流體光碟檢測.................................................................................................... 15
第三章實驗方法.......................................................................................................... 16
3.1實驗藥品與材料.................................................................................................... 16
3.2微流體光碟製作.................................................................................................... 16
3.3微流體碟片觀測系統............................................................................................ 17
3.3.1旋轉運動系統 ................................................................................................. 17
3.3.2影像擷取系統 ................................................................................................. 19
3.3.3傾注量分析系統 ............................................................................................. 19
3.4光學檢測系統........................................................................................................ 20
3.4.1 光學檢測平台 ................................................................................................ 20
3.4.2 光學訊號分析方法 ........................................................................................ 21
第四章實驗結果與討論.............................................................................................. 22
4.1 基礎條件探討....................................................................................................... 22
4.1.1 樣本之選擇 .................................................................................................... 22
4.1.2 光學路徑 ........................................................................................................ 23
4.1.3 詴劑用量 ........................................................................................................ 24
4.2 微流體功能........................................................................................................... 25
4.2.1 傾注量探討 .................................................................................................... 26
4.2.2 混合條件探討 ................................................................................................ 28
4.2.3氣泡清除時間 ................................................................................................. 30
4.3 化學反應............................................................................................................... 31
4.3.1 反應濃度對反應時間之影響 ........................................................................ 31
4.4 臨床檢測............................................................................................................... 33
第五章結論.................................................................................................................. 35
第六章未來展望.......................................................................................................... 35
參考文獻...................................................................................................................... 35

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