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研究生:劉冠岑
論文名稱:健身器材製造廠勞工之職業暴露與健康傷害關係之評估
論文名稱(外文):Evaluation of the association between occupational exposure and health effects of the workers in industry of manufacturing fitness equipments
指導教授:陳秀玲陳秀玲引用關係
學位類別:碩士
校院名稱:弘光科技大學
系所名稱:職業安全與防災研究所
學門:環境保護學門
學類:環境防災學類
論文種類:學術論文
畢業學年度:100
語文別:中文
論文頁數:111
中文關鍵詞:健身器材氧化性傷害肺部發炎指標重金屬
外文關鍵詞:fitness equipmentsoxidative damagemetalslung inflammation
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摘要
運動器材製造過程包含器具切割、噴漆、粉體塗裝、銲接、零件組裝、拋光、品保品管等,其中以銲接作業與粉體塗裝為主要之工作型態,勞工所暴露之危害物質也較為廣泛,包含金屬粉塵、活性含氧物種 (Reactive oxygen species, ROS)等。
本研究以外銷健身器材之製造公司為主要研究場所,進行健身器材作業勞工之生物暴露偵測,如分析血液重金屬,而勞工之生物效應偵測則評估氧化傷害 (MDA, DNA strand breaks, telomere length)、肺部發炎指標 (TNF-α)、心血管疾病指標 (hs-CRP)、染色體端粒長度(Telomere length)及相對風險估計之評估。
勞工之肺部發炎反應指標TNF-α及MDA之分析結果顯示暴露組勞工有顯著偏高於對照組勞工;Telomere length之分析結果則相反之,此結果顯示此類型之作業場所,勞工之職業暴露可能已經造成體內氧化傷害、發炎反應與Telomere length變短。此外,個人習慣、服務年資、粉塵暴露與佩戴防護具可能為干擾因子,而這些干擾因子會影響勞工體內氧化傷害及TL。此外,於製造部門勞工無配戴防護具者其血中重金屬Cr、Fe、Co、Cu、Zn、Mn、Cd、Mg、As濃度高於製造部門勞工有配戴防護具者,此結果顯示現場勞工如有配戴呼吸防護具,其體內重金屬均有顯著較低之現象。

此外,本研究結果顯示Mg暴露可能為勞工Telomere length變短之保護因子,而Fe暴露會導致勞工相對風險估計值下降。根據研究顯示染色體端粒變短與心血管疾病、高密度膽固醇累積與增加氧化傷害有關,因此本研究結果也可推論此類型之職業暴露會增加勞工未來罹患相關健康疾患之可能性。

關鍵詞:健身器材,氧化性傷害,肺部發炎指標,重金屬


Abstract
The main production route of manufacturing fitness equipments industry includes cutting appliances, painting, welding, assembling parts, polishing, quality assurance quality control, welding jobs and painting. Therefore, the workers exposed to many hazardous substances, including metal dust and reactive oxygen species (ROS).
In the present study, the analysis of the blood heavy metals and biological effects of the oxidative damage(MDA, DNA strand breaks and telomere length assessment), the lung inflammatory markers (TNF-α), indicators of cardiovascular disease (hs-CRP), chromosome telomere length was done in an export company of manufacturing fitness equipments.
The significantly higher levels of TNF-α and MDA analysis was found in exposure group than those of the control, but telomere length analysis of the results was contrary. Meanwhile, the results showed that the workplaces, occupational exposure of the labor may caused the oxidative damage, and the shorten of telomere length. In addition, personal habits, working year, dust exposure and wearing protective equipment might be the confounding factors, to affect the oxidative injury and TL shorten. In the manufacturing workers, the levels of blood heavy metals, Cr, Fe, Co, Cu, Zn, Mn, Cd, Mg and As seemed to be lower in the workers wearing protective equipments than those did not wear protective equipments. These results indicated that the wearing a respirator was a protective factor for preventing the heavy metals exposure.
In addition, the result of this study showed that the Mg exposure might be a factor for protecting the shortening of telomere length of workers. Research has demonstrated that chromosome telomere shortening was in related to the cardiovascular disease. High-density lipoprotein cholesterol accumulation was in related to increased oxidative damage of this study can be inferred that this type of occupational exposure will increase the possibility of the workers suffering cardiovascular disorders in the future.

Keywords: fitness equipments, oxidative damage, metals, lung inflammation

摘要 I
Abstract III
誌謝 V
目錄 VII
表目錄 X
圖目錄 XII
第一章 緒論 1
1-1 研究背景 1
1-2 研究目的 2
第二章 文獻回顧 3
2-1健身器材業介紹 3
2-2重金屬 10
2-3生物指標 12
2-3-1脂質過氧化反應產物(Malondialdehyde, MDA) 12
2-3-2血液DNA傷害(tail moment) 13
2-3-3染色體末端長度(telomere length) 14
2-4肺部發炎指標 16
腫瘤壞死因子(tumor necrosis factor α, TNF-α) 16
2-5心血管疾病指標 17
高敏感性C-反應蛋白(high sensitivity C-reactive protein, hs-CRP) 17
2-6動物實驗與流行病學研究結果 18
第三章 研究材料與方法 22
3-1研究架構 22
3-2研究對象 23
3-3 血液MDA之實驗方法 23
3-3-1血液MDA分析方法 23
3-3-2檢量線製作 25
3-4 Comet assay分析 25
3-4-1血液採集 25
3-4-2 Comet分析方法 26
3-4-3影像分析與計算 27
3-5染色體端粒長度(telomere length)分析 29
3-5-1血液採集 29
3-5-2 telomere length分析方法 29
3-6肺部發炎反應:TNF-α 33
3-6-1血液採集 33
3-6-2 TNF-α分析方法 33
3-6-3檢量線製作 34
3-7心血管疾病前軀指標:hs-CRP 36
3-7-1血液採集 36
3-7-2 hs-CRP分析方法 36
3-7-3檢量線製作 37
3-8血液重金屬之實驗方法 38
3-8-1血液採集 38
3-8-2樣品前處理 38
3-8-3血液重金屬分析方法 38
3-8-4品保品管 40
3-9 勞工健康問卷調查 44
3-10 統計分析方法 45
3-10-1描述性統計 50
3-10-2分析性統計 51
第四章 結果與討論 52
4-1勞工之基本資料 52
4-2健身器材勞工血液MDA、DNA傷害濃度、hs-CRP、TNF-α及TL長度之分布情形 58
4-3健身器材勞工個人生活習慣因素對MDA、DNA傷害濃度、TL、TNF-α及hs-CRP之影響 60
4-4勞工血液MDA、DNA傷害濃度、hs-CRP、TNF-α及TL長度、相對風險估計之相關 68
4-5 MDA、TMOM、hs-CRP、TNF-α及TL長度之複回歸分析 70
4-6血液重金屬 74
4-6-1 不同部門之勞工血液重金屬分析 74
4-6-2勞工血液重金屬濃度與防護具使用分析 77
4-6-3勞工氧化性傷害與血液重金屬相關性分析 79
第五章 結論與建議 82
5-1結論 82
5-2建議 83
第六章 參考文獻 84

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