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研究生:張育萍
論文名稱:氧化銦錫製造廠作業勞工銦暴露與健康傷害之追蹤研究
論文名稱(外文):Follow up of indium exposure and health illness of the workers in indium tin oxide production plants
指導教授:陳秀玲陳秀玲引用關係
學位類別:碩士
校院名稱:弘光科技大學
系所名稱:職業安全與防災研究所
學門:環境保護學門
學類:環境防災學類
論文種類:學術論文
畢業學年度:100
語文別:中文
論文頁數:86
中文關鍵詞:氧化銦錫氧化性傷害脂質過氧化反應KL-6SP-D
外文關鍵詞:indium-tin oxideoxidative damageKL-6SP-D
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隨著國內高科技產業的快速發展,半導體與液晶面板製程技術日新月異,其中主要原料氧化銦錫的使用量更是與日俱增。現代工業廣泛的將金屬銦應用在製作液晶顯示器、平板顯示器、電漿顯示器、觸碰式顯示器、電子紙、光電導體、合金製造、太陽能等高科技領域。由於氧化銦錫製造廠作業勞工在工作過程中會暴露於金屬銦,且國內外文獻之研究報告亦證實氧化銦錫及銦化合物會對人體產生危害,然目前有關氧化銦錫製造廠作業勞工之健康危害的追蹤調查報告與文獻資料依然十分缺乏,使氧化銦錫製造廠及其相關產業在進行作業勞工的健康評估時缺乏相關之毒理資料資訊,因此本研究經由追蹤氧化銦錫製造廠作業勞工之銦暴露與健康危害以進行健康效應之探討。
本研究主要藉由血液之DNA斷裂(DNA strand breaks, comet assay)、脂質過氧化反應濃度(Malondialdehyde, MDA)、間質肺部疾病(Krebs von den Lungen, KL-6)、表面活性劑蛋白質(Surfactant Protein-D, SP-D)及血清銦測量結果,以追蹤氧化銦錫廠勞工之血清銦與氧化性傷害、蛋白質指標之差異與相關性,並探討年齡、年資、菸酒習慣及職業暴露對於勞工體內血清銦、氧化性傷害、脂質過氧化反應及蛋白質指標之差異與相關性。
本研究在氧化銦錫廠共有94位勞工參加,採集每位勞工之血液,血清銦濃度使用感應耦合電漿質譜儀(Inductively Coupled Plasma Mass Spectrometry, ICP-MS)進行分析,採樣二年血清銦濃度分別為1.6μg/l 、1.0μg/l、1.2μg/l,及利用單細胞電泳法分析DNA stand breaks(tail moment),使用螢光分光光譜儀於測量血中脂質過氧化反應指標MDA濃度,利用ELISY酵素免疫分析法分析勞工血清中KL-6及SP-D濃度。
本研究結果顯示抽菸、喝酒與運動習慣不會對於血清銦、氧化傷害(tail moment、MDA)及蛋白質(KL-6、SP-D)造成顯著性差異。追蹤暴露銦之勞工二年,蛋白質(KL-6)之濃度明顯增加,是為間質性肺部疾病之敏感指標。血清銦濃度在不同年資及不同部門有顯著性差異。血清銦與脂質過氧化(MDA)及蛋白質(KL-6、SP-D)有顯著性相關。顯示本研究之結果確實可提供氧化銦錫製造廠及其相關產業在進行作業勞工之健康評估時的考證與依據資訊。

Due to the rapid development of flat panel and liquid crystal displays (LCD), indium-tin oxide (ITO) is becoming increasingly used in the production process. ITO is a mixture of indium-oxide and tin-oxide; the most common use of ITO is in LCDs as a transparent electrical conductor and inflector in the production of LCD monitors, flat panel displays, plasma displays, and other high-technology fields. The previous reports have showed that ITO and indium compounds would cause adverse health effects to workers who exposed to them. However, the relationship from follow up study is lack on indium exposure and health illness in indium-tin oxide production plants. Therefore, this study aims to follow up the exposure of indium and health effects of workers in indium-tin oxide production plants.
In this study, ninety-four workers were recruited to follow up their serum indium concentration by using Inductively Coupled Plasma Mass Spectrometry ICP-MS for 2 years. Meanwhile, plasma lipid peroxidation was measured as the MDA level by a fluorescence spectrophotometer. The tail moment was considered as DNA strand breaks by comet assay (single cell gel electrophoresis or SCEG). Krebs von den Lungen (KL-6), Surfactant Protein-D (SP-D) was analyzed by using a competitive ELISA immunoassay.
The results of this study showed that no significant difference of serum indium, oxidative damage (tail moment, MDA), and protein (KL-6, SP-D) between smoker and non-smokers, as well as their drinking habit in the significantly differences were found in the follow up data of indium, protein (KL-6) concentration. The data showed that KL-6 might be a sensitive indicator of interstitial lung disease. Between different work years and departments, the serum indium concentration had significant difference. The serum indium was significantly correlated to MDA, KL-6 and SP-D. Therefore, the results of this study can provide information of the health assessment to workers in indium-tin oxide manufacturing plant and its related industries.

目錄
誌謝 I
中華民國一○一年八月二日摘要 II
Abstract V
目錄 VII
表目錄 X
圖目錄 XI
第一章 緒論 1
1-1研究背景 1
1-2研究目的 3
第二章 文獻回顧 5
2-1氧化銦錫簡介 5
2-2金屬銦之健康危害 6
2-3生物指標 8
2-3-1脂質過氧化反應產物丙二醛(Malondialdehyde, MDA) 8
2-3-2 DNA strand breaks (tail moment) 10
2-3-3 Krebs von den Lungen(KL-6) 11
2-3-4 Surfactant Protein-D(SP-D) 12
2-4銦之職業暴露 13
第三章 材料與方法 15
3-1研究架構 15
3-2研究對象 15
3-3勞工之健康問卷調查 16
3-4 血液MDA分析 17
3-4-1 血液採集 17
3-4-2血液MDA分析方法 17
3-4-3檢量線製作 18
3-5 DNA strand breaks(tail moment)分析 19
3-5-1血液採集 19
3-5-2 DNA strand breaks(tail moment)分析方法 19
3-5-3影像分析與計算 21
3-6 血清銦分析 22
3-6-1血液採集 22
3-6-2 血清銦分析分法 22
3-6-3檢量線製作 23
3-7 Krebs von den Lungen(KL-6)分析 24
3-7-1血液採集 24
3-7-2 KL-6分析分法 24
3-7-3 檢量線製作 25
3-8 Surfactant Protein-D(SP-D)分析 26
3-8-1血液採集 26
3-8-2 SP-D分析分法 26
3-8-3 檢量線製作 28
3-9統計分析 28
3-9-1描述性統計 28
3-9-2單變項分析 29
3-9-3多變項分析 29
第四章 結果與討論 30
4-1 勞工個人基本資料 30
4-2勞工基本資料對勞工血清銦、氧化性傷害及蛋白質濃度之差異 31
4-3不同生活習慣的勞工血清銦、氧化性傷害及蛋白質之差異 32
4-4追蹤血清銦、氧化性傷害及蛋白質之結果 33
4-5血清銦與氧化性傷害及蛋白質之差異與相關性 35
第五章 結論與建議 39
5-1結論 39
5-2建議 40
參考文獻 41

(一)期刊
Alfantazi , Moskalyk ., 2003. Processing of indium: a review. Minerals Engineering 687-694.
Asnar, J., Santos, M. J., Valles, J., Sala, J., 1983. Serum malondialdehyde-like material(MDA-LM)in acute myocardial infarction. J Clin Pathol 36:712-715.
Blazka, M. E., Dixon, D., et al., 1994. Pulmonary toxicity to intratracheally administered indium trichloride in Fischer 344 rats. Fundam Appl Toxicol 22: 231-239.
Block, G., Dietrich, M., Norkus, E. P., Morrow, J. D., Hudes, M., Caan, B., Packer, L., 2002. Factors Associated with Oxidative Stress in Human Populations. American Journal of Epidemiology 156: 274-85.
Carolyn, F.M., Urmila, P.K., et al., 2002. Toxicologyic pathology. 30: 427.
Chonan, T., Taguchi, O., et al., 2007. Interstitial pulmonary disorders in indium-processing workers. Eur Respir J. 29: 317-324.
Chou, W.C., Chio, C.P., Liao, C.M., 2009. Assessing airborne PM-bound arsenic epxosure risk in semiconductor manufacturing facilities. J Hazard Mat 167: 976-986.
Chuang, C. H., Hu, M. L., 2004. Use of whole blood directly for single-cell gel electrophoresis (comet) assay in vivo and white blood cells for in vitro assay. Mutation Research 564: 75-82.
Cummings, K. J., Donat, W. E., et al., 2010. Pulmonary alveolar proteinosis in workers at an indium processing facility. Am J Respir Crit Care Med 181:458-464.
Crouch, E. C., 1998.Collectins and pulmonary host defense. American journal of respiratory cell and molecular biology 19: 177-201.
Garaj-Vrhovac, V. and Zeljezic, D., 2000. Evaluation of DNA damage in workers occupationally exposed to pesticides using single-cell gel electrophoresis (SCGE) assay. Pesticide genotoxicity revealed by comet assay. Mutat Res 469:279-85.
Hamaguchi, T., Omae, K., et al., 2008. Exposure to hardly soluble indium compounds in ITO production and recycling plants is a new risk for interstitial lung damage. Occup Environ Med 65: 51-55.
Homma, S., Miyamoto, A., et al., 2005. Pulmonary fibrosis in an individual occupationally exposed to inhaled indium-tin oxide. Eur Respir J. 25: 200-204.
Hong, T., Qin, D., et al., 2004. Indium market and the characteristics of indium resources in china. Yunnan Geographic Environment Research 3.
Humad, S., Zarling, E. J., Skosey, J. I., 1985. lipid peroxidation in rheumatoid arthritis : measurement of pentane in breath samples by gas chromatography. Clin Res 33:919A.
Kretzschmar, M. and Muller, D. 1993. Aging, training and exercise. A review of effects on plasma glutathione and lipid peroxidation. Sports Medicine. 15:196-209.
Ledwozyw, A., Michalak, J., Stepien, A., Kadziolka, A.,1986. The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis, Clinica Chemica Acta 155:275-284.
Liao, Y.H., Yu, H.S., Ho, C.K., Wu, M.T., Yang ,C.Y., Chen ,J.R., Chang, C.C., 2004. Biological monitoring of exposures to aluminium, gallium, indium, arsenic, and antimony in optoelectronic industry workers. J Environ Occ Med 46: 831-936.
Liao, Y.H., Hwang ,L.H., Kao, J.S., YiiH ,S.J., Lin ,S.F., Lin, C.H., Lin ,Y.C., Aw, T.C., 2006. Lipid peroxidation in workers exposed to aluminium, gallium, indium, arsenic, and antimony in the optoelectronic industry. J Occ Environ Med 48: 789-793.
Liu, H. H., Chen, C. Y., et al., 2012. Relationship between indium exposure and oxidative damage in workers in indium tin oxide production plants. International Archives of Occupational and Environmental Health 85: 447-453.
Lykkesfeldt, J., 2007. Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking. Clinica Chimica Acta 380: 50-58.
Nishigake, I., Hagihara, M., Tsunekawa, H., Maseki, M., Yagi, K.,1981. Lipid peroxide levels of serum lipoprotein fractions of diabetic patients. Biochem Med 25:373-378.
Ostling, O. and Johanson, K., 1984. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochemical and Biophysical Research Communications 123: 291-298.
Sakuma, T., Takahashi, K., et al., 1999. Serum KL-6, a novel mucin-like glycoprotein, as an indicator of interstitial pneumonitis following lobectomy. Surgery today 29: 121-128.
Spiteller, G., et al., 2001.Lipid peroxidation in aging and age-dependent diseases. Experimental Gerontology 36: 1425-57.
Stahel, R. A., Gilks, W. R., et al., 1994. Third international workshop on lung tumor and differentiation antigens: overview of the results of the central data analysis. International Journal of Cancer 57: 6-26.
Taguchi, O. and Chonan, T., 2006. Three cases of indium lung. Nihon Kokyuki Gakkai Zasshi 44: 532-536.
Tice, R.R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J. C. and Sasaki, Y. F., 2000. Single cell gel/Comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35:206-21.
Tseng, F.M., Chiu, Y.J., Chen, J.S., 2007. Measuring business performance in the high-tech manufacturing industry: Acase study of Taiwan’s large-sized TFT-LCD panel companies. Omega.
Valavanidis, A., Vlahogianni ,T., Dassenakis, M., Scoullos, M., 2006. Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants. Ecotoxicology and Environmental Safety 64: 178-189.
Vivo, Genetic .,et al.,Toxicology Testing. Environmental and Molecular Mutagenesis 206-221.
Wang, G.S., Deng, Y.C., Lin, T.F., 2007. Cancer risk assessment from trihalomethanes in drinking water. Sci Total Environ 387: 86-95.
Wiklund, S. J. and Agurell, E., 2003. Aspects of design and statistical analysis in the Comet assay. Mutagenesis 18:167-75.
Zhu, C. Q., Lam, T. H., Jiang, C. Q., Wei, B. X., Xu, Q. R. And Chen, Y. H., 2000. Increased lymphocyte DNA strand breaks in rubber workers. Mutat. Res. 470:201-209.
Zhu, C. Q., Lam, T. H., Jiang, C. Q., 2001. Lymphocyte DNA damage in bus manufacturing workers. Mutat. Res./Genet. Toxicol. Environ. Mutagenesis 491: 173-181.
(二)學位論文
劉柏青,2008,「電銲金屬燻煙暴露之氧化傷害評估」,碩士論文,弘光科技大學職業安全與防災研究所,台中。
黃衍翔,2010,「以茶葉廢渣及電混凝技術去除水溶液中鎵、銦離子之研究」,碩士論文,弘光科技大學職業安全與防災研究所,台中。
黃崇恩,2009,「電銲金屬燻煙暴露之氧化傷害評估」,碩士論文,弘光科技大學職業安全與防災研究所,台中。
張雅婷,蕭庭哲,吳俊毅, 2008, 「銦金屬資源與日前資源化處理技術之探討」, 資源與環境學術研討會論文集,花蓮,第291-300頁。
陳成裕,2008,「銦、氧化銦錫及銦化合物暴露危害預防」,勞工安全衛生研究所,簡訊第96期。
陳怡如,2008,「底渣處理廠及飛灰固化處理廠勞工體內脂質過氧化反應及氧化性傷害之研究」,碩士論文,弘光科技大學職業安全與防災研究所,台中。
楊健宏,2012,「鑄造廠及塑膠廠PAHs與重金屬暴露與氧化傷害之相關性探討」,碩士論文,弘光科技大學職業安全與防災研究所,台中。

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