跳到主要內容

臺灣博碩士論文加值系統

(3.236.84.188) 您好!臺灣時間:2021/08/04 22:56
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:葉怡君
研究生(外文):YEH,YI CHUN
論文名稱:比較小球藻 裂片石蓴 皺紫菜於美容用途及機制之區別
論文名稱(外文):Chlorellaceae,the Ulva fasciata,Porphyra crispata kjellman in the difference between cosmetic purposes and mechanisms.
指導教授:邵東子姜泰安姜泰安引用關係
指導教授(外文):Dong Zi ShaoTai An Chiang
口試委員:林指宏邵東子姜泰安
口試委員(外文):Jju Home LinDong Zi ShaoTai An Chiang
口試日期:2012-06-27
學位類別:碩士
校院名稱:中華醫事科技大學
系所名稱:醫學檢驗生物技術系碩士班
學門:醫藥衛生學門
學類:醫學技術及檢驗學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:80
中文關鍵詞:小球藻裂片石蓴皺紫菜保濕抗氧化
外文關鍵詞:ChlorellaceaeUlva fasciataPorphyra crispata kjellmanMoisture retentionAntioxidant activity
相關次數:
  • 被引用被引用:1
  • 點閱點閱:248
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
  本研究是利用綠藻中最大生物裂片石蓴(Ulva fasciata)及最小生物小球藻(Chlorellaceae)將二者以不同的萃取方法進行比較,同時也對尚未在美粧界開發的皺紫菜(Porphyra crispata kjellman)進行探討,小球藻以溫度120℃萃取,酒精沉澱(小球藻I型,Chlo-lacⅠ)及85℃萃取,酒精沉澱(小球藻Ⅱ型,Chlo-lac Ⅱ);裂片石蓴120℃萃取,酒精沉澱(裂片石蓴Ⅰ型,Ulva-fasⅠ)及120℃萃取,冷凍乾燥 (裂片石蓴Ⅱ型,Ulva-fasⅡ)、皺紫菜萃取出紫菜油(Por-cri)分別探討這些萃取物之保濕性、抗氧化活性及細胞存活率之影響。
  保濕性測定包括水合作用及經皮水散失值(TEWL)。抗氧化活性測定包括DPPH自由基清除力、螯合亞鐵離子能力、還原力測定。細胞存活率測定,MTT assay。
保濕性測定中,水合作用結果顯示只有小球藻表現出水合保濕效果。而實驗結果證實三種海藻都具有穿皮失水率障壁功能,更讓人注目的是以冷凍乾燥萃取的裂片石蓴Ⅱ型,保濕障壁功能更強於酒精沉澱的裂片石蓴Ⅰ型。
  在抗氧化活性中,濃度於10mg/ml的條件下,小球藻Ⅰ型和Ⅱ型的DPPH清除率達67~71%,二者幾乎相同;裂片石蓴Ⅰ型表現強於裂片石蓴Ⅱ型。
螯合亞鐵離子實驗中,在濃度10mg/ml條件下,小球藻Ⅰ型Ⅱ型的螯合能力分別是65%及63% ;裂片石蓴Ⅰ型Ⅱ型分別是55%及57%;皺紫菜可達到66%,在還原力的實驗中,小球藻Ⅱ型的還原力是93%強於I型55%的還原力。裂片石蓴在DPPH自由基清除能力和還原力的實驗結果中,濃度於10mg/ml裂片石蓴Ⅱ型的清除率強於裂片石蓴Ⅰ型。
  在細胞存活率實驗中,藻類萃取液的濃度,從不加藥到10mg/ml的條件下,結果顯示Ha Cat、B16、Hela細胞的存活率基本上沒有什麼改變,證實這些藻類萃取液對細胞幾乎沒有殺傷作用。
  以上結果為海藻在美容、防止老化及臨床應用提供了良好的科學依據,也證實了作為化妝品基質的可行性。
  This study tries to make a comparison between ulva fasciata the largest class of green algae and chlorellaceae the smallest class of green algae by using different extraction methods, and also explores the effect of porphyra crispata kjellman that have not yet been developed in beauty and make-up field. To probe into the effect of algae on moisture retention, antioxidant activity and cell viability, chlorellaceae are extracted under 120℃ (chlo-lacⅠ) and 85℃ (chlo-lacⅡ) and settle in alcohol, while ulva fasciata are extracted under 120℃ and settle in alcohol (ulva fasciataⅠ) or freeze dried (ulva fasciataⅡ), por-cri oil are extracted from porphyra crispata kjellman.
  Moisture retention tests cover hydration and transepidermal water loss (TEWL) values. Antioxidant activity tests involve diphenyl picryl hydrazinyl radical scavenging capacity, Fe2+-chelation ability, reducing power, cell viability and MTT assay.
In moisture retention tests, hydration results show that only chlorellaceae have hydrated moisture effect. Experimental results indicate the TEWL barrier function of the three kinds of marine algae. More significantly, ulva fasciataⅡ extracted by the freeze drying method are superior to ulva fasciata Ⅰ settled in alcohol in terms of moisture retention barrier effect.
In antioxidant activity tests under conditions of the 10mg/ml concentration, chlorellaceae Ⅰ and Ⅱ have almost the same DPPH scavenging rate of 67~71%; ulva fasciata Ⅰ is better than ulva fasciataⅡ.
  In Fe2+ chelation ability tests under conditions of the 10mg/ml concentration, the chelation capabilities of chlorellaceae Ⅰ and Ⅱ are 65% and 63% respectively; ulva fasciata Ⅰ and Ⅱ’s chelation capabilities are 55% and 57% respectively, while porphyra crispata kjellman’ chelation capability reaches 66%. In reducing power tests, chlorellaceae Ⅱ is more powerful with 93% reducing power than chlorellaceaeⅠ with 55% reducing power. DPPH radical scavenging capacity and reducing power tests for ulva fasciata under the 10mg/ml concentration show that ulva fasciata Ⅱ have more scavenging capacity than ulva fasciata Ⅰ .
  In cell viability tests of the algae extracts under the concentration of zero to 10mg/ml, results show that basically the viability of Ha Cat, B16 and Hela cells has few changes, indicating these extracts have hardly any killing effect on cells.
The above results provide a solid scientific basis for application of marine algae in beauty, anti-aging and clinical fields, and also confirm the feasibility of marine algae as cosmetics base materials.
中文摘要……………………………………………………………iv
英文摘要........................................vi
謝誌………………………………………………………………viii
目錄............................................ix
表目錄.........................................xiii
圖目錄..........................................xiv
第一章 序論
第一節 前言……………………………………………………1
第二節 研究動機與目的………………………………………3
第二章 文獻整理
第一節 海藻介紹………………………………………………4
一、何謂藻類…………………………………………………5
二、藻類的分佈與種類………………………………………5
三、綠藻之相關介紹…………………………………………5
四、紅藻之相關介紹…………………………………………6
五、小球藻之介紹……………………………………………8
六、裂片石蓴之介紹……………………………………… 10
七、皺紫菜之介紹………………………………………… 12
第二節 人體皮膚結構
一、表皮層……………………………………………………14
二、真皮層……………………………………………………15
三、皮下組織…………………………………………………15
第三節 皮膚老化
一、皮膚老化的特徵………………………………………..16
二、皮膚老化的相關機制……………………………………16
三、皮膚障壁功能……………………………………………17
四、紫外線與皮膚老化之關係………………………………17
第四節 自由基
一、自由基分類………………………………………………19
二、自由基與活性氧對生物體之危害………………………20
三、自由基與老化之相關性…………………………………21
第五節 人體中的抗氧化系統
一、酵素性抗氧化系統……………………………………21
二、非酵素性抗氧化系統…………………………………22
第三章 材料與方法
第一節 實驗架構………………………………………………26
第二節 實驗材料與方法
一、實驗儀器與材料………………………………………28
二、抗氧化功能測定試劑……………………………………28
三、細胞培養與分析之藥品…………………………………29
第三節 實驗方法
一、保濕功能測定…………………………………………30
(一)水合作用測定…………………………………………30
(二)經皮水分散失值測定…………………………………31
二、抗氧化功能測定…………………………………………32
(一)DPPH自由基清除能力測定……………………………32
(二)螯合亞鐵離子能力測定………………………………33
(三)還原力測定……………………………………………34
三、細胞培養……………………………………………35
(一)附著性細胞之培養……………………………………35
(二)細胞的繼代培養………………………………………35
(三)細胞解凍與保存………………………………………36
四、細胞存活率實驗…………………………………………36
(一)MTT assay……………………………………………………37
第四節 統計分析………………………………………38
第四章 結果與討論
第一節 保濕有效性評估
一、水合作用……………………………………………………38
二、經皮水分散失值…………………………………………..39
第二節 抗氧化測試
一、DPPH 自由基清除能力測定……………………………40
二、螯合亞鐵離子能力測定…………………………………41
三、還原力測定………………………………………………42
第三節 細胞存活率
MTT assay………………………………………………43
第五章 結論…………………………………………………………45
第六章 參考文獻…………………………………………………46


1.Bernard, P. and Berthon, J. Y.( 2000) Resveratrol: an original mechanism on tyrosinase inhibition. International Journal of Cosmetic Science. 22: 219-226.
2.Bold, H. C. and M. J. Wynne,(1985) Introduction to algae, Prentice Hall Inc.
3.Burton GW, Ingold KU,(1984) beta-Carotene:an unusual type of lipid antioxidant,Science 224:569-73.
4.Byorksten, J. A. (1958) A common molecular basis for the agingsyndrome. Journal of the American Geriatrics Society. 6: 740.
5.Cameron E, Pauling L(1976) Supplemental ascorbate in thesupportive
treatment of cancer: Prolongation of survival times in terminal human
cancer.Proc Natl Acad Sci USA 73: 3685-9.
6.Carr A, Frei B. (1999) Does vitamin C act as a pro-oxidant under phys iologyical con-ditions Faseb J 13:1007-24.
7.Carr AC, McCall MR, Frei B(2000) Oxida-tion of LDL by myelop-eroxidase and reactive nitrogen species: reaction pathways and antioxidant protection.Arterioscler Thromb Vasc Bio 20: 1716-23.
8.Davies, K. J. A., Qaintanilha, A. T.,Brooks,G. A.and Packer, L.(1982) Freeradicals and tissue damage produced by exercise. Biochemical and Biophysical Research Communications. 107(4):1198-1205.
9.Del Caro A., Piga A., Pinna I., Fenu P. M., Agabbio M., (2004) Effect of Drying Conditionsand Storage Period on Polyphenolic Content, Antioxidant Capacity, and Ascorbic Acid of Purnes, Journal of Agricultural and Food Chemistry, 52: 4780-4784.
10.Dinis T. C. P., Madeira V. M. C., Almeida L. M., (1994) Action of Phenolic Deriva-tives(Acetaminophen, Salicylate, and 5-Aminosalicylate)as Inhibit-ors of Membrane Lipid Peroxidation and as Peroxyl Radical Scavengers , Archives of Biochemistry and Biophysics315:,161-169.
11.Dozg zi shao,Cheng kun wang,Hann jang hwang,Ching hsia hung,Yu wen chen (2010) Comparison of hydration,tyrosinase resistance,and antioxidant activation in three kinds of pearl powders.61,133-145.
12.Eric,FB,Jouni, U.(1996) Clinics in Dermatology.14:143.151.
13.Harman D. Aging(1956) a theory based on free radical and radiation chemistry. J Gerontol 11: 298-300.
14.Hink U, Li H, Mollnau H, et al.(2001) Mechanisms underlying
endothelialdysfunction in diabetes mellitus. Circ Res 88: E14-22.
15.Jenkins, G.,Molecular mechanisms of skin ageing. (2002)Mechanis-ms ofAgeing and Development. 123: 802-810.
16.Jung, J. W., Cha, S. H., Lee, S. C., Chun, I. K. and Kim Y. P.(1997) Age-related changes of water content in the rat skin. Journal of Dermat ological Science.14:12-19.
17.Kovacic P, Pozos RS, Somanathan R,Shangari N, O'Brien PJ.(2005)
Mechanism of mitochondrial uncouplers, inhibitors,and toxins: focus onelectron transfer,free radicals, and structure-activity Curr Med Chem 12:2601-23.
18.Kukreja RC, Hess ML.(1992) The oxygen free radical system: fromequations through membrane-protein interacti ons to cardi ovascul arinjur yand protection. Cardiovasc Res 26:641-55.15.
19.Lee R.E., (1989) Chlorophyta. In Phycology. Cambridge University Press, New York. P224-228.
20.Li JM, Shah AM.( 2003) ROS generation by nonphagocytic NADPH
oxidase:po-tential relevance In diabetic nephropa-thy. J Am SocNephr ol 14:S221-6.
21.Likhitwitayawuid K., Stilbenes with tyrosinase inhibitory activity,Current Science,94(1): 44-51, 2008.
22.McCord JM, Fridovich I.(1969) Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 244: 6049-55.
23.Mauricio, G. Y.(1996) Clinics in Dermatology.14:153-160.
24.Nahm, W. K., Zhou, L. and Falanga, V.( 2002) Sustained ability forfibr oblast outgrowth from stored neonatal foreskin: a model for studyingmec-hanisms of fibroblast outgrowth. Journal Dermatologi calScience.28:P152-158.
25.Oyaizu M,( 1986) Antioxidative activity of browning products of glucosmine fractionated by organic solvent and thin-layer chromato graphy .Nippon shokuhin Kogyo Gakkaishi 35:771-775.

26.Pinnagoda J, Tupker RA, Agner T and Serup J. (1990) "Guidelines fortransepidermal water loss (TEWL) measurement. A report from the Standardization Group of the European Society of Contact Dermatitis."Contact Derm 22(3):164-78.
27.Pitotti, A., Elizalde, B. E. and Anese, M. (1995) Effect of caramelization and maillard reaction products on peroxidase activity. J. Food Biochem. 18(6):445-457.
28.Pastor N, Weinstein H, Jamison E, Brenowitz M.( 2000) A detailedin terpr etation of OH radical footprints in a TBP-DNA complex reveals therole of dynamics in the mechanism of sequencespecic binding . JMol Biol304:55-68.
29.Pastore A, Federi ci G, Bert i ni E,Piemonte F.(2003) Analysis of glutathione:implication in redox and detoxication.Clin Chim Acta 333:19-39.
29.Prota, G. (1996) Melanins and Melanogenesis. Cosmetic and Tolletries Magazine. 111: 43-51.
30.Quanbin Zhang, Ning Li , Gefei Zhou, Xiaolan Lu, Zuhong Xu, Zhien Li , (2005) In vivo antioxidant activity of polysaccharide fraction from Porphyra haitanesis (Rhodephyta) in aging mice.
31.Robertson RP, Harmon J, Tran PO,Tanaka Y, Takahashi H. (2003) Glucosetoxicity in beta-cells:type 2 diabetes,good radicals Gone bad,and theglutathione connection . Diabetes 52:581-7.
32.Roberts MS and Walker M. Water. The most natural penetratione
nhancr. In: Walters KA, Hadgraft J (eds). (1993) Pharmaceutical SkinPenetration Enhancement. New York., Marcel Dekker.
33.Roberts MS and Walker M. Water. "The most natural penetrationenhancer."In: Walters KA, Hadgraft J (eds). (1993) Pharmaceutical Skin Penetration Enhancement. New York., Marcel Dekker.
34.Rodriguez R.J., Miranda C.L., Stevens J.F., Deinzer M.L., Buhler D.R., (2001) Influence of prenylated and non-prenylated flavonoids on liver microsomal lipid peroxidation and oxidative injury in rat hepatocytes.Food Chem. Toxicol., 39(5), 437-445.
35.Stadtman ER.( 2004) Role of oxidant species in aging. Curr Med Chem11:1105-12.
36.Sies H, Stahl W, Sundquist AR. (1992) Antioxidant functions of vitamins. Vitamins E and C, beta-carotene, and other carotenoids. Ann N Y Acad Sci669: 7-20.
37.Schaefer, H., and Redelmeier, T. E. (1996) Skin barrier: Principles of percutaneous absorption. Karger, New York.
38.Snadra,M.D.L;Nico,P.M;MoniqueV.V.(2001),Journal of Photobiology B: Biology.61: 106-113.
39.Shampa, M.;FSamir, K. M. and Ranjan, B.(2002) Human placental lipidinduces mitogenesis and melanogenesis in B16 F10 melanoma cells.J. Biosci.27:243-249.
40.Yin-Ta Chen; Yung-Hsiang Tsai,(2006) Hygienic quality and
antioxidanta ctivities of commerical microalgal products in Taiwan.
41.Yamaguchik.(1997) Recent advance in microalgal bioscience in Japan,with special reference to utilization of biomass and metabolites: a review[J].J Appl Phyc , 8(6): 487-502.
42.Valko M, Leibfritz D, Moncol J, et al. (2007) Free radicals and antioxidantsin nor-mal physiological functions and human disease. Int J Biochem CellBiol 39: 44-84.
43.Val ko M, I zakovic M, Mazur M,Rhodes CJ, Telser J.( 2004) Roleof oxygen radicals in DNA damage and cancer incidence. Mol Cell Biochem266: 37-56.
44.Wester RC and Maibach HI. (1995) Percutaneous Penetration
Enhancers. Boca Raton FL, CRC Press.
45.Wickett RR, Nath V, Tanaka R and Hoath SB: Use of continuous
electrical capacitance and transepidermal water loss measurements for
assessing barrier function in neonatal rat skin. Skin Pharmacol. 8:
179-85, 1995.
46.Y.L.Lee,M.T.Yen,and J.L.Mau, (2007) ,Antioxdant propertiens of variousextracts from Hypsizigu mar-moreus,Food Chem.,104,1-9 .
47.Pinnagoda, J, Tupker, RA, Agner, T and Serup J. (1990)〝Guidelines for transepidermal water loss (TEWL) measurement.A report from the Standar dization Group of the European Society of Contact Dermatitis.〞,Contact Derm. 22(3): 164-178.
48.Prota, G. (1996) Melanins and Melanogenesis. Cosmetic and Tolletries Magazine. 111: 43-51.
49.方銘志,(2002),山藥多醣體之免疫調節機能探討,國立台灣海洋
大學水產食品科學研究所碩士論文。
50.光井武夫(陳韋達、鄭慧文譯),(1992),新化妝品學,南山堂
株式會社出版,合記圖書出版社發行,P13-46。
51.孔維寶、李龍囡、張繼、夏春谷,(2010),小球藻的營養保健功能
及其食品工業的應用,P323-327。
52.李時珍,本草綱目(2002),北京,華夏出版社。
53.林春吉,(2005),台灣的水生與溼地植物,台北,綠世界出版社。
54.周子傑,(2005),二硫化碳暴露對皮膚疾病與皮膚障壁功能損失之
探討,國立成功大學基礎醫學研究所博士論文。
55.陳宜嫻,(2001),皮膚黑色素抑制劑與保濕劑效能之研究,靜宜大
學應用化學研究所博士論文,P35。
56.黃淑芳,(1997),台灣的海藻資訊及利用。台灣博物。15: 76-87。
57.黃淑旻,(2004),評估豬隻胎盤萃取液在皮膚老化及美白之功效,
國立交通大學生化工程研究所碩士論文,P9-13,23-24。
58.楊佳璋等合著(陳宜嫻、傅如嶽、黃宜純、黃淑桂、楊佳璋、溫慧
萍、鄭智交),(2004),皮膚生理學,華格那出版社,二版,P45-51。
59.楊濟華,(1995),部分中草藥美白與防曬成分研究,靜宜大學應用
化學研究所碩士論文,P18。
60.趙強,(1997),自由基,美食天下,64:P116。
61.臺博館臺灣海藻資訊網
62.德永和信與研谷啟,(1993),90 年代における新製品開發-老化防
止化妝品,Frangrance Journal,21(1):P51-56 。
63.黎孝韻、曾國慶,(2008),自由基及抗氧化物功能的探討,藥學雜
誌臨床醫學24:P95-101。

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top