跳到主要內容

臺灣博碩士論文加值系統

(3.236.110.106) 您好!臺灣時間:2021/07/24 05:42
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:黃兆尉
研究生(外文):Chao-Wei Huang
論文名稱:篩選細菌素生產益生菌及中草藥對其抗菌能力之影響
論文名稱(外文):Screening Of Bacteriocin Producing Probiotic And Effect Of Herbal Medicines To Its Antimicrobial Ability
指導教授:呂英震
指導教授(外文):Ying-Chen Lu
口試委員:吳志忠謝淑玲
口試委員(外文):Chih-Chung WuShu-Ling Hsieh
口試日期:2012-06-26
學位類別:碩士
校院名稱:中華醫事科技大學
系所名稱:生物醫學研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:77
中文關鍵詞:中草藥益生菌細菌素
外文關鍵詞:herbal medicinesprobioticbacteriocin
相關次數:
  • 被引用被引用:3
  • 點閱點閱:617
  • 評分評分:
  • 下載下載:97
  • 收藏至我的研究室書目清單書目收藏:0
近年來,益生菌改善人體健康已受到相當的重視。乳酸菌及比非德氏菌是最常見也是潛力的益生菌。在發酵食品中,乳酸菌亦扮演最核心的角
色,其所產生的抗菌蛋白或胜肽稱為細菌素,具有展現抑制食品腐敗菌的活性。利用乳酸菌發酵中草藥具有許多優點,例如:提高中草藥之萃取率、
產生新的活性成分、減少廢棄物等。本研究從實驗室菌種庫中總共分析了270 株乳酸菌,其中以 Lactobacillus plantarum 居多,利用洋菜槽擴散法
從中選取具有產生細菌素之乳酸菌,經過序列比對後,鑑定此菌株為Lactobacillus gasseri。由實驗結果發現,Lactobacillus gasseri 的菌數在 MRS
培養基下培養 16 小時達到 2.1×108 ,20 小時後菌數開始往下降。培養8小時後,細菌素的抑制活性即可達到最高峰,以 Fast Protein Liquid
Chromatography(FPLC)分析,fraction7、8 具有抑制活性,以 Proteinase K處理後失去活性,經過 110℃處理 10 分鐘後具有抑制活性。由此本研究進一步使用了 11 種不同的中草藥進行發酵,結果發現 Lactobacillus gasseri經由不同的中草藥發酵後,對 Lactobacillus gasseri 的生長及細菌素的產生會有不同的影響,經過山苦瓜及知母發酵之後,Lactobacillus gasseri產生的細菌素對 E. coli 及 S. aureus 還保有活性,而隨著濃度的提高,菌數及抑菌圈也隨之增加。探討中草藥對 Lactobacillus gasseri 產生細菌素的影響,推測有部分中草藥會吸附 Lactobacillus gasseri 所產生的細菌素,以及中草藥會影響 Lactobacillus gasseri 產生細菌素之合成,經實驗後,證實山藥與黃精會吸附細菌素,而其他中草藥影響細菌素的原因還有待探討。
In recent years, probiotics improve human health has received considerable attention. Lactic acid bacteria including lactobacilli and bifidobacteria are the most common bacterial species considered as potential
probiotics . Lactic acid bacteria have an essential role in the majority of food fermentations , Its produce antimicrobial proteins and peptides called bacteriocins , It was shown to inhibit food spoilage microorganisms . The use of lactic acid bacteria fermented Chinese herbal medicine has many advantages , Such as : increase the extraction rate of Chinese Herbal Medicine、Production of new active ingredients、Reduce waste, etc . This study analyzed a
total of 270 lactic acid bacteria in the laboratory culture collections, lactic acid bacteria were selected and screened for their ability to produce bacteriocin by agar well diffusion method, after the Sequence Alignment, identification of this strain is Lactobacillus gasseri. In this study, The cell density of Lactobacillus
gasseri increased to 2.1×108 during 16 h, after the 24h the bacterial count started to decrease. bacteriocin production was observed at 8 hours, The best activity in inhibition of bacteriocin in 24 hours , Analysis by Fast Protein Liquid Chromatography (FPLC) , whereas the peak of bacteriocin was observed at fraction 7、8 , The activity of bacteriocin was destroyed after treatment with Proteinase K, bacteriocin remained active after 10 min at 110℃.
This study used 11 different Chinese herbs medicine for fermentation, The results showed that Lactobacillus gasseri through the fermentation of Chinese herbal medicine, the growth and bacteriocin of Lactobacillus gasseri, they
would have different effects, after Momordica charantia and Anemarrhena fermentation, bacteriocin produced by Lactobacillus gasseri still retained antimicrobial activity against E. coli and S. aureus, With the increase of
Chinese herbs medicine concentration, Bacterial counts and the inhibitory activity also increases. The Exploration of Chinese herbs medicine to produce bacteriocin by Lactobacillus gasseri, Speculate that there are some Chinese
herbal medicine will be the adsorption of bacteriocins produced by Lactobacillus gasseri, and Chinese herbal medicine will change the Lactobacillus gasseri to produce bacteriocin synthesis pathway, After the experiments, Confirmed that the Dioscorea opposita and Polygonatum sibiricum adsorption of the bacteriocin, and other Chinese herbal medicine
bacteriocin reasons yet to be explored.
目錄 ....................................................................................................... I
中文摘要 .............................................................................................. II
英文摘要 ............................................................................................ III
壹、導論 .............................................................................................. 1
貳、研究構想 .................................................................................... 18
參、材料與方法 ................................................................................ 19
肆、結果 ............................................................................................ 23
伍、討論 ............................................................................................ 26
陸、結論 ............................................................................................ 27
柒、參考文獻 .................................................................................... 28
捌、表 ................................................................................................ 36
玖、圖 ................................................................................................ 38
拾、附表 ............................................................................................ 56
- 30 -

柒、參考文獻

李時珍著,御史編譯。本草綱目:天下第一藥典(白話精譯本)。大地出版社。
(2008)
廖永安。中醫藥材使用寶鑑。億(王民)文化。(2007)
林欣慧。膳食中草藥對乳酸菌菌體生長之影響,碩士論文,朝陽科
龍湘美。 乳酸菌細菌素抗菌作用及其在食品上應用之回顧 國立台灣海
洋大學食品科學系碩士論文。 (2004)
國學書院系列編委會,楊玲宜。本草綱目。西北國際文化。(2009)
科技大學應用化學系研究所。(2004)
黃國欽。以中藥為益生素提升乳酸菌調節膽固醇能力,碩士論文,中國醫
藥大學。(2004)
黃崇真。 腸道微生物與益生菌。 食品工業。 (2004)
黃則棓。乳酸菌粉劑試量產-菌種及培養基之探討,碩士論文,朝陽
技大學應用化學系研究所。(2007)
邱雪惠。乳酸菌之抗癌機制.。食品工業, 36, 16. (2004)
余秋樺。評估乳酸菌改善腸道發炎之作用,碩士論文,中華醫事科技大學
生物液學研究所。(2010)
楊媛絢。原生保健性菌種與益菌助生質之應用。 食品工業。 (1997)
Aaron I, Mark F, Robert JM and Mark T (2003) The bacteriocin piscicolin 126
retains antilisterial activity in vivo. J. Antimicrob. Chemother. 51 (6):
1365-1371.
Abee T, Klaenhammer TR and Letellier L (1994) Kinetic studies of the action
of lactacin F, a bacteriocin produced by Lactobacillus johnsonii that
forms poration complexes in the cytoplasmic membrane. Appl Environ
Microbiol, 60(3), 1006-1013. - 31 -

Abee T, Krockel L and Hill C (1995) Bacteriocins: modes of action and
potentials in food preservation and control of food poisoning. [Review].
Int J Food Microbiol, 28(2), 169-185.
Adolfsson O, Meydani SN and Russell RM (2004) Yogurt and gut function.
Am J Clin Nutr. 80, 245-256.
Alvarez-Olmos MI and Oberhelman RA (2001) Probiotic agents and infectious
diseases: a modern perspective on a traditional therapy. Clin Infect Dis.
2001 Jun 1;32(11):1567-76.
Alvarez-Olmos MI and Oberhelman RA (2001) Probiotic Agents and
Infectious Diseases:A Modern Perspective on a Traditional Therapy.
Clinical Infectious Diseases. 2001; 32:1567–76
Arakawa K, Kawai Y, Ito Y, Nakamura K, Chujo T, Nishimura J, Kitazawa H
and Saito T (2010) HPLC purification and re-evaluation of chemical
identity of two circular bacteriocins, gassericin A and reutericin 6. Lett
Appl Microbiol, 50(4), 406-411.
Arunachalam KD (1999) Role of bifidobacteria in nutrition, Medicine and T
echnology. Nutr. Res. 19, 1559–1597.
Baricault L, Denariaz G, Houri JJ, Bouley C, Sapin C and Trugnan G (1995)
Use of HT-29, a cultured human colon cancer cell line, to study the
effect of fermented milks on colon cancer cell growth and differentiation.
Carcinogenesis. Feb;16(2):245-52.
Baricault L, Denariaz G, Houri JJ, Bouley C, Sapin C and Trugnan G (1995)
Use of HT-29, a cultured human colon cancer cell line, to study the
effect of fermented milks on colon cancer cell growth and
differentiation. Carcinogenesis. Feb;16(2):245-52. - 32 -

Bartram HP, Scheppach W, Gerlach S, Ruckdeschel G, Kelber E and Kasper H
(1994) Does yogurt enriched with Bifidobacterium longum affect
colonic microbiology and fecal metabolites in health subjects? Am J
Clin Nutr, 59(2), 428-432.
Bernet MF, Brassart D, Neeser JR and Servin AL (1994) Lactobacillus
acidophilus LA 1 binds to cultured human intestinal cell lines and
inhibits cell attachment and cell invasion by enterovirulent
bacteria. Gut, 35, 483–489.
Bezirtzoglou E and Stavropoulou E (2011) Immunology and probiotic impact
of the newborn and young children intestinal microflora. Anaerobe.
Dec;17(6):369-74. Epub 2011 Apr 16.
Bonadè A, Murelli F, Vescovo M and Scolari G (2001) Partial characterization
of a bacteriocin produced by Lactobacillus helveticus. Lett Appl
Microbiol, 33(2), 153-158.
Breukink E and de Kruijff B. (1999).The lantibiotic nisin, a special case or not?
Biochim Biophys Acta . Dec 15;1462(1-2):223-34.
Broadbent JR, Chou YC, Gillies K and Kondo JK (1989) Nisin inhibits several
gram-positive, mastitis-causing pathogens. J Dairy Sci. 1989
Dec;72(12):3342-5.
Brook I (1999) Bacterial Interference.Critical Reviews in Microbiology, Vol.
25, No.3, pp. 155-172
Chen Y and Montville TJ (1995) Efflux of ions and ATP depletion induced by
pediocin PA-1 are concomitant with cell death in Listeria
monocytogenes .Scott A. J Appl Microbiol, 79(6), 684-690. doi:
10.1111/j.1365-2672.1995.tb00955.x - 33 -

Chikindas ML, García-Garcerá MJ, Driessen AJ, Ledeboer AM, Nissen-Meyer
J, Nes IF, Abee T, Konings WN and Venema G (1993) Pediocin PA-1,
a bacteriocin from Pediococcus acidilactici PAC1.0, forms hydrophilic
pores in the cytoplasmic membrane of target cells. Appl Environ
Microbiol, 59(11), 3577-3584.
Cudic M and Otvos L (2002) Intracellular targets of antibacterial peptides.
Current Drug Targets, 3, 101-106
Davey GP and Richardson BC (1981) Purification and Some Properties of
Diplococcin from Streptococcus cremoris 346. Appl Environ Microbiol,
41(1), 84-89.
Deegan LH, Cotter PD, Hill C and Ross P (2006) Bacteriocins: Biological tools
for bio-preservation and shelf-life extension. International dairy journal
/ published in association with the International Dairy Federation, 16(9),
1058-1071.
Frazier WC and Westhoff DC (1988) Microorganisms important in food
microbiology.McGraw-Hill Book Co, New York, pp. 35-97.
Fukushimk M, Yamada A, Endo T, Nakano and M (1999) Effects of a mixture
of organisms, Lactobacillus acidophilus or Streptococcus faecalis on
1976-desaturase activity in the livers of rats fed a
fat-and-cholesterol-enriched diet. Nutrition. 15, pp. 373–378.
Fuller, R. (1989) Probiotics in man and animals. J Appl Bacteriol. 1989
May;66(5):365-78.
Galdeano CM, LeBlanc M, Vinderola G, Bibas MEB and Perdigón G (2007)
Proposed model: mechanisms of immunomodulation induced by
probiotic bacteria. Clin Vaccine Immunol. 14, 485–492. - 34 -

Ganjam LS, Thorntonm WH, Marshall RT and Macdonald RC (1997)
Antiproliferative effects of yogurt fractions obtained by membrane
dialysis on cultured mammalian intestinal cells. J Dairy Sci. 80,
2325–2329.
Gonzalez B, Glaasker E, Kunji E, Driessen A, Suarez J E and Konings WN
(1996) Bactericidal mode of action of plantaricin C. Appl Environ
Microbiol, 62(8), 2701-2709.
González Martínez BE, Gómez TM and Jiménez SZ (2003)
BACTERIOCINAS DE PROBIÓ TICOS. Salus cum propositum vitae
Vol 4 No.2 Abril-Junio
Hilton E, Kolakowski P, Singer C and Smith,M (1997) Efficacy of
Lactobacillus GG as a Diarrheal Preventive in Travelers. J Travel Med,
4(1), 41-43.
Huang Y and Zheng Y (2010) The probiotic Lactobacillus acidophilus reduces
cholesterol absorption through the down-regulation of Niemann-Pick
C1-like 1 in Caco-2 cells. Br J Nutr, 103(4), 473-478. doi:
10.1017/s0007114509991991
Ingham A, Ford M, Moore R J and Tizard M (2003) The bacteriocin piscicolin
126 retains antilisterial activity in vivo. Comparative Study
International Journal of Medical Microbiology 300 (2010) 57–62
Jalonen T, Isolauri E, Heyman M, Crain-Denoyelle AM, Sillanaukee P
and Koivula T(1991) Increased beta-lactoglobulin absorption during
rotavirus enteritis in infants: relationship to sugar permeability.
Pediatr Res. 1991 Sep;30(3):290-3. - 35 -

Jalonen T, Isolauri E, Heyman M, Crain-Denoyelle AM, Sillanaukee P and
Koivula T (1991) Increased beta-lactoglobulin absorption during
rotavirus enteritis in infants: relationship to sugar permeability.
Pediatr Res. Sep;30(3):290-3.
Joerger MC and Klaenhammer T R (1986) Characterization and purification of
helveticin J and evidence for a chromosomally determined bacteriocin
produced by Lactobacillus helveticus 481. J Bacteriol, 167(2),
439-446.
Kaur I , Chopra K and Saini A (2002) Probiotics: potential pharmaceutical
Applications. Eur. J. Pharm. Sci. 15, 1–9
Kawai Y, Ishii Y, Arakawa K, Uemura K, Saitoh B, Nishimura J and Saito T
(2004) Structural and functional differences in two cyclic bacteriocins
with the same sequences produced by lactobacilli. Appl Environ
Microbiol, 70(5), 2906-2911.
Kim DS, Kim SH, Kim BK, Yang MC and Ma JY (2012) Antiasthmatic
Effects of Herbal Complex MA and Its Fermented Product MA128.
Evid Based Complement Alternat Med. 769508. Epub 2011 Nov 15.
Klaenhammer T R, Fremaux C and Hechard Y (1994) activité
antimicrobienne des bactéries lactiques. In-Bactéries Latiques, H.
De Roissart and F. M. Luquet, Lorica. .
Koort J, Murros A, Coenye T, Eerola S, Vandamme P, Sukura A and Björkroth
J (2005) Lactobacillus oligofermentans sp. nov., Associated with
Spoilage of Modified-Atmosphere-Packaged Poultry Products. Appl
Environ Microbiol. 2005 Aug;71(8):4400-6. - 36 -

Lavermicocca P, Valerio F, Evidente A, Lazzaroni S, Corsetti A and Gobbetti,
M (2000) Purification and characterization of novel antifungal
compounds from the sourdough Lactobacillus plantarum strain 21B.
Appl Environ Microbiol, 66(9), 4084-4090.
Lee, Y.K. and S. Salminen(1995) The coming of age of probiotics. Trends
Food Sci. Technol., 6: 241-245.
Lievin V, Peiffer I and Houdijk AP (2000) Bifidobacterium strains from
resident infant human gastrointestinal microflora exert antimicrobial
activity,Gut, Vol. 47, pp. 646-652
Lilly DM. and Stillwell RH (1965) Probiotics.Growth promoting factors
produced by micro-organisms. Science 147, 747-748.
LIN MY and CHEN TW (2000) Reduction of Cholesterol by Lactobacillus
acidophilus in Culture. Journal of Food and Drug Analysis.
macrophage cells. BMC Complementary and Alternative Medicine ,
12:17
Liu SN, Han Y and Zhou ZJ(2011) Lactic acid bacteria in traditional fermented
Chinese foods. Food Research International 44 .643–651.
Luc DV and Erick JV (1994) Bacteriocins of Lactic Acid Bacteria:
Microbiology, Genetics and Applications. Blackie Academic and
Professional.
Makras L, Triantafyllou V, Fayol-Messaoudi D, Adriany T, Zoumpopoulou
G, Tsakalidou E, Servin A and De Vuyst L (2006) Kinetic analysis of
the antibacterial activity of probiotic lactobacilli towards Salmonella
enterica serovar Typhimurium reveals a role for lactic acid and other
inhibitory compounds.. Res Microbiol, 157(3), 241-247. doi: - 37 -

10.1016/j.resmic.2005.09.002
Marra G and Boland CR (1995) Hereditary nonpolyposis colorectal cancer: the
syndrome, the genes, and historical perspectives. J Natl Cancer Inst,
87(15), 1114-1125.
McAuliffe O, Ross RP and Hill C (2001) Lantibiotics: structure, biosynthesis
and mode of action. FEMS Microbiol Rev, 25(3), 285-308. Microbiol
Infect. 2005; 11:958–66.
Meydani SN and HaWK. (2000). Immunologic effects of yogurt. Am J Clin
Nutr. 71, 861–872.
Montville T J and Bruno M E (1994) Evidence that dissipation of proton
motive force is a common mechanism of action for bacteriocins and
other antimicrobial proteins. myths. Med J Aust. 2008; 188:304–8.
Montville TJ and Bruno ME (1994) Evidence that dissipation of proton motive
force is a common mechanism of action for bacteriocins and other
antimicrobial proteins. Int J Food Microbiol. Dec;24(1-2):53-74.
Naidu AS, Bidlack WR and Clemens RA (1999) Probiotic spectra of lactic
Probiotic spectra of lactic acid bacteria (LAB).Crit Rev Food Sci
Nutr. Jan;39(1):13-126.
Nancy TW (2010) Probiotics. American Journal of Health-System
Pharmacy. March 15, vol. 67 no. 6449-458
Nes IF and Holo H (2000) Class II antimicrobial peptides from lactic acid
bacteria. Peptide Science, 55(1), 50-61. doi:
10.1002/1097-0282(2000)55:1<50::aid-bip50>3.0.co;2-3
Oh YC, Cho WK, Oh JH, Im GY, Jeong YH, Yang MC and Ma JY (2012)
Fermentation by Lactobacillus enhances anti-inflammatory - 38 -

effect of Oyaksungisan on LPS-stimulated RAW 264.7 mouse
macrophage cells. BMC Complement Altern Med. Mar 12;12:17.
Orrhage K, Sillerstrom E, Gustafsson JA, Nord CE and Rafter J (1994) Binding
of mutagenic heterocyclic amines by intestinal and lactic acid bacteria.
Mutat Res, 311(2), 239-248.
Ouwehand AC, Salminen S and Isolauri E (2002) Probiotics: an overview of
beneficial effects. Antonie Van Leeuwenhoek. Aug;82(1-4):279-89.
Parada JL, Caron CR, MEDEIROS ABP and Soccol CR (2007) Bacteriocins
from Lactic Acid Bacteria: Purification,Properties and use as
Biopreservatives. Brazilian Archives of Biology and Technology 50(3),
521-542.
Parvez S, Malik KA, Ah Kang S and Kim HY (2006) Probiotics and their
fermented food products are beneficial for health. [Review]. J Appl
Microbiol, 100(6), 1171-1185. doi: 10.1111/j.1365-2672.2006.02963.x
Pham M, Lemberg DA and Day AS (2008) Probiotics: sorting the evidence
from the myths. Med J Aust 2008; 188 (5): 304-308.
Pool-Zobel B, Van LJ, Rowland I and Rober FB ( 2002) Experimental
evidences on the potential of prebiotic fructans to reduce the risk of
colon cencer. Br. J. Nutr. 87(Suppl.2): 273-281.
Rafter J (2002) Lactic acid bacteria and cancer: mechanistic perspective. Br J
Nutr, 88 Suppl 1, S89-94. doi: 10.1079/BJN2002633
Renye JA Jr and Somkuti GA (2010) Nisin-induced Expression of Pediocin
in Dairy Lactic Acid Bacteria. Journal of Applied Microbiology.
108:2142-2151.
Rowland IR, Rumney CJ, Coutts JT and Lievense LC (1998) Effect of - 39 -

Bifidobacterium longum and inulin on gut bacterial metabolism and
carcinogen-induced aberrant crypt foci in rats. Carcinogenesis, 19(2),
281-285.
Sahl HG and Bierbaum G (1998) Lantibiotics: biosynthesis and biological
activities of uniquely modified peptides from gram-positive bacteria.
Annual Review of Microbiology Vol. 52: 41-79
Santosa S, Farnworth E and Jones PJ (2006) Probiotics and their potential
health claims. Nutr Rev. 64(6):265-74.
Schnürer J and Magnusson J (2005) Antifungal lactic acid bacteria as
biopreservatives. Trends in Food Science andamp; Technology,
16(1–3), 70-78. doi: 10.1016/j.tifs.2004.02.014
Senok AC, Ismaeel AY and Botta GA(2005) Probiotics: facts and myths. Clin
Microbiol Infect. Dec;11(12):958-66. Review.
Shornikova AV, Casas IA, Isolauri E, Mykkänen H and Vesikari T (1997)
Lactobacillus reuteri as a therapeutic agent in acute diarrhea in young
children. J. Pediatr. Gastroenterol. Nutr. 24: 399-404.
Simpson PJ, Stanton C, Fitzgerald GF and Ross RP (2002) Genomic Diversity
within the Genus Pediococcusas Revealed by Randomly
Amplified Polymorphic DNA PCR and Pulsed-Field Gel
Electrophoresis. Appl. Environ. Microbiol.February 2002 vol.
68no. 2 765-771
Strom K, Sjogren J, Broberg A and Schnurer J (2002) Lactobacillus plantarum
MiLAB 393 produces the antifungal cyclic dipeptides
cyclo(L-Phe-L-Pro) and cyclo(L-Phe-trans-4-OH-L-Pro) and
3-phenyllactic acid. Appl Environ Microbiol, 68(9), 4322-4327. - 40 -

Tagg JR and McGiven AR (1971) Assay system for bacteriocins. Appl
Microbiol, 21(5), 943.
Tobias A. Oelschlaeger (2010) Mechanisms of probiotic actions – A review.
Int J Med Microbiol.300(1):57-62.
Upreti GC and Hinsdill RD (1975) Production and mode of action of lactocin
27: bacteriocin from a homofermentative Lactobacillus. Antimicrob
Agents Chemother, 7(2), 139-145.
Vaughan EE, Daly C and Fitzgerald GF (1992) Identification and
characterization of helveticin V-1829, a bacteriocin produced by
Lactobacillus helveticus 1829. [Research Support, Non-U.S. Gov't]. J
Appl Bacteriol, 73(4), 299-308.
Venema K, Chikindas M L, Seegers J, Haandrikman AJ, Leenhouts KJ,
Venema G and Kok J (1997) Rapid and Efficient Purification Method
for Small, Hydrophobic, Cationic Bacteriocins: Purification of
Lactococcin B and Pediocin PA-1. Appl Environ Microbiol, 63(1),
305-309.
Waleed KM,Al-Tememy, Ibraem ,Al-Ani, Mohammed Q, Al-Ani, Nadia T,
Al-Alusi and Salmah Ismail (2011) Isolation of Lactobacillus
salivarius from Children and Purification of Bacteriocin to Inhibition
Cancer Cell in Vitro. Hotel Equatorial Bangi-Putrajaya, Malaysia, 14 -
15 January
WHO and FAO (2001) Health and Nutritional Properties of Probiotics in Food
including Powder Milk with Live Lactic Acid Bacteria. Amerian
Zhang M, Qiao X, Zhao L, Jiang L, Ren F (2011) Lactobacillus salivarius REN
counteracted unfavorable 4-nitroquinoline-1-oxide-induced changes in colonic microflora of rats. J Microbiol, 49(6), 877-883. doi:
10.1007/s12275-011-1137-7
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top