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研究生:江怡宣
研究生(外文):I-Shiuan Chiang
論文名稱:開發暫時性蛋白質錨定系統可用於高通量分選具最高蛋白質產量之細胞
論文名稱(外文):Development of a transiently protein-anchored system for high-throughput sorting of the most productive protein-producing cell
指導教授:鄭添祿
指導教授(外文):Tian-Lu Cheng
學位類別:碩士
校院名稱:高雄醫學大學
系所名稱:生物醫學暨環境生物學研究所
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:英文
論文頁數:33
中文關鍵詞:暫時性蛋白質錨定技術流式細胞分選技術高產能細胞重組蛋白藥物
外文關鍵詞:transiently protein-anchored systemfluorescence activated cell sorting (FACS)high-producing cellrecombinant protein
相關次數:
  • 被引用被引用:0
  • 點閱點閱:105
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1. ABSTRACT 3
2. INTRODUCTION 6
3. MATERIALS AND METHODS 9
3.1. MATERIALS AND REAGENTS 9
3.2. CELLS AND ANIMALS 9
3.3. PLASMID CONSTRUCTION 9
3.4. GENERATION OF ANTI-EGFR ANTIBODY-SECRETING CELL BY LENTIVIRAL TRANSDUCTION 10
3.5. WESTERN BLOTTING ANALYSIS 10
3.6. FLOW CYTOMETRY ANALYSIS 11
3.7. ELISA ANALYSIS 12
4. RESULTS 13
4.1. CHARACTERIZATION OF TRANSIENTLY PROTEIN-ANCHORED SYSTEM MODULATED BY THE FURIN INHIBITOR 13
4.2. CORRELATION OF MEMBRANE-ANCHORED ANTI-EGFR AB-RAKR-B7 EXPRESSION WITH SECRETED ANTI-EGFR ANTIBODY TITERS 14
4.3. SOLUBLE LYSOSOMEAL PROTEIN 15
5. DISCUSSION 18
6. FIGURES 23
6.1. THE TRANSIENTLY PROTEIN-ANCHORED SYSTEM. 23
6.2. FURIN INHIBITOR-MEDIATED SWITCH OF SECRETED ANTI-EGFR ANTIBODY AND ANCHORED ANTI-EGFR AB-RAKR-B7 24
6.3. RELEVANCE BETWEEN THE SECRETED ANTI-EGFR AB AND THE MEMBRANE-ANCHORED ANTI-EGFR AB-RAKR-B7 26
6.4. CALCULATION OF THE CORRELATION COEFFICIENT BETWEEN THE SECRETED ANTI-EGFR AB AND THE MEMBRANE-ANCHORED ANTI-EGFR AB-RAKR-B7 27
6.5. ACCURATE PREDICTION OF THE CLONES PRODUCING HIGH LEVELS OF RECOMBINANT LYSOSOMAL PROTEINS. 28
7. REFERENCE 30


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