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研究生:洪堉智
研究生(外文):Yu-Chih Hung
論文名稱:基於單轉軸之稜鏡耦合表面電漿共振量測系統之研製
論文名稱(外文):Development of Prism-coupled Surface-plasmon-resonance Measurement System Based on Single-axis Rotation
指導教授:易政男
指導教授(外文):Jenq-Nan Yih
學位類別:碩士
校院名稱:國立高雄應用科技大學
系所名稱:電機工程系
學門:工程學門
學類:電資工程學類
論文種類:學術論文
論文出版年:101
畢業學年度:100
語文別:中文
論文頁數:49
中文關鍵詞:表面電漿共振單軸旋轉生物感測器牛血清蛋白
外文關鍵詞:Surface Plasmon Resonance (SPR)Single-axis RotationBiosensorBovine Serum Albumin (BSA)
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我們研製一套單轉軸之稜鏡耦合表面電漿共振 (Surface Plasmon Resonance, SPR) 的光學量測系統,並進行基本性能測試與生物感測實驗。本系統之設計為簡化雙轉軸為單轉軸,量測過程中只有單一轉台轉動以改變入射角;此外,同時分別使用參考光與參考感測區,以消減光源強度不穩與機構重置所引入的雜訊。用 LabVIEW 控制系統、處理數據,並顯示結果在儀控介面上。數據處理包含將光偵測器所接收到的訊號經過菲涅耳方程 (Fresnel equations) 換算到晶片反射光強度之結果、擬合原始數據,和利用衰減全反射 (Attenuated Total Reflection) 找出反射率最低點的表面電漿共振角 (SPR angle)。經由系統穩定性測試實驗,我們得到系統的表面電漿共振角之重複性為 0.015±0.002 度。在生物分子感測實驗部分,以牛血清蛋白(Bovine Serum Albumin, BSA)作為目標生物分子,並與自組裝單分子層 (Self-assembled Monolayer) 進行交互作用,依序反應 1、2、4、6、8、10及15 分鐘,並記錄其SPR角偏移量;結果顯示,濃度為 0.1 mg/ml 的 BSA 溶液在感測表面上反應約 15 分鐘後,就已達到飽和,其 SPR 角偏移約 0.64 度。上述實驗結果證明,本論文所提之單轉軸之稜鏡耦合表面電漿共振感測系統具有高穩定性,並且可應用在分析生物分子交互作用之動態資訊。
We assembled an optical measurement system of prism-coupled surface plasmon resonance (SPR) based on single-axis rotation, tested its basic performance with a biomolecular interaction. The advantage of system is to simplify two-axis rotation design to a single-axis one. When measuring, one single-axis turntable changes the incident angle; moreover, adding a reference beam and a reference sensing area simultaneously can decrease the noises induced by the instability of laser intensity and the reset of system mechanism. Using a LabVIEW programming language to control the system, process data, and show results at graphic interface. Data processing includes that a conversing the received signals from photodetectors into the reflectivity of total-internal-reflection through Fresnel equations, fitting the raw data, locating the angle position at minimum reflectivity as the SPR angle. Through the testing experiments for system stability, we obtained the repeatability of SPR angle for the system is ca. 0.015±0.002 degrees. In the biomolecular sensing experiment, a bovine serum albumin (BSA) adopted as a target molecule to be immobilized on self-assembled monolayer (SAM). The SPR angles were measured sequentially at the interacted time 1, 2, 4, 6, 8, 10 and 15 minutes. The obtained results show that the SPR angle shifts increase rapidly in the beginning and then reach a stable value after saturation time. The saturation time is ca. 15 minutes at the BSA concentration of 0.1 mg/ml, and the corresponding SPR angle shift is ca. 0.640 degrees. These experimental results show that the proposed SPR measurement system based on single-axis rotation has high stability and can be used for analyzing the biomolecular interaction.
摘要 VI
ABSTRACT VII
誌謝 IX
目 次 X
圖 次 XII
表 次 XIV
第一章 緒論 1
1.1 前言 1
1.2 研究動機與目的 3
1.3 文獻回顧 3
1.4 論文架構 4
第二章 表面電漿 5
2.1 表面電漿共振現象理論 5
2.2 激發表面電漿子機制 13
第三章 單轉軸表面電漿共振量測系統之設計與製作 15
3.1 系統設計 15
3.2 軟體界面介紹 21
3.3 實驗架構製作與量測 24
3.4 實驗流程說明 31
第四章 實驗結果分析與比較 34
4.1 實驗結果 34
4.2 SAM與BSA交互作用 43
4.3 實驗量測結果與討論 45
第五章 結論 46
參考文獻 47
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