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研究生:許筑維
研究生(外文):Hsu,chu-wei
論文名稱:不同碳源或氮源影響北蟲草於綠茶液生長及生長期時期綠茶發酵液之功能性能力變異之探討
論文名稱(外文):Investigation of Cordyceps militaris growth cultured in green tea liquid and functional variation of fermented liquid during growth period effected by various carbon or nitrogen source.
指導教授:王盛世
指導教授(外文):Wang,shan-shue
口試委員:張芳榮白育綸王盛世
口試日期:2012-06-08
學位類別:碩士
校院名稱:高苑科技大學
系所名稱:化工與生化工程研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:94
中文關鍵詞:北蟲草菌綠茶泡液碳源氮源蟲草素腺苷茶多酚
外文關鍵詞:Cordyceps militarisgreen tea soaking liquidcarbon sourcenitrogen sourceadenosinecordycepintea polyphenol
相關次數:
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  • 下載下載:46
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本研究以北蟲草菌(Cordyceps militaris)培養於添加不同比例蔗糖或脫脂乳之綠茶葉浸泡液進行發酵。我們發現北蟲草菌均可於所有測試條件下正常生長。適當的添加碳源有助於綠茶浸泡液之北蟲草菌生長數目,於6%的砂糖有最好生長的情形。但添加過多的氮源於茶葉浸泡液,不會明顯增加蟲草的生長數量。於綠茶發酵液進行不同功能性能力分析發現,所有的含脫脂乳綠茶發酵液裡,發現都有很高的DPPH自由基清除能力,介於9.4至11.7 mg Trolox/mL;於所有的含蔗糖綠茶發酵液裡,也都發現都有很高的DPPH自由基清除能力,介於8.4至10.5 mg Trolox/mL;於所有的含脫脂乳綠茶發酵液裡,發現都有很顯著量的總多酚,介於1.88至2.40 mg Gallic acid/mL;於所有的含蔗糖綠茶發酵液裡,發現都有很顯著量的總多酚,介於1.79至2.35 mg Gallic acid/mL;於所有的含脫脂乳綠茶發酵液裡,發現都有的總醣量,介於4.9至5.9 mg Glucose/mL; 含2%與4%蔗糖之綠茶發酵液的總糖含量於第30天生長期已減少至與控制組的總糖含量相近為有可接受的少糖量。以HPLC分析北蟲草發酵液於發酵期間其核苷酸含量之變化情形。於含脫脂乳發酵液所發現的蟲草素濃度介於0.05至1.75 mg/mL之間和含蔗糖發酵液所發現的蟲草素濃度介於0.16至0.79 mg/mL之間。所有添加脫脂乳與添加蔗糖的茶葉浸泡液均含有顯著量的腺苷量。於含脫脂乳發酵液所發現的腺苷濃度介於0.48至4.86 mg/mL之間和含蔗糖發酵液所發現的腺苷濃度介於1.41至4.82 mg/mL之間。以HPLC分析茶多酚,也發現到所有北蟲草發酵液裡,皆含有顯著量的茶多酚分怖。由上述資料的呈現,以北蟲草菌培養於含蔗糖的綠茶浸泡液,可促進北蟲草菌生長的數量,並於發酵液顯示出含有良好的功能性成分與能力,有潛力成為一優良之保健飲料。
Cordyceps (Cordyceps militaris) was cultured in green tea soaking liquid added with various ratio of sucrose or skim milk in this study. We found that Cordyceps fungi have demonstrated to be normally grown in all test conditions of green tea soaking liquid. The carbon sources added appropriately in green tea soaking liquid can improve the number of Cordyceps fungus growth, which has the best growth at 6% sucrose, but not in extra nitrogen source. The green tea fermentative liquid were analyzed their functional capabilities. All green tea fermentation broth containing skim milk or sucrose were found to have a high DPPH radical scavenging ability, ranging from 9.4 to 11.7 mg Trolox / mL or from 8.4 to 10.5 mg Trolox / mL; All green tea fermentation broth containing skim milk or sucrose were found to have a very significant amount of total polyphenols, ranging from 1.88 to 2.4 mg Gallic acid / mL or from 1.79 to 2.35 mg Gallic acid / mL; The total amount of sugar were ranged 4.9 to 5.9 mg glucose / mL among all green tea fermentation broth containing skim milk. In comparison with control, the total sugar amount of 2% and 4% sucrose green tea fermentation liquid at 30 days' growth were decreased to the level of control, which is acceptable. The nucleotide content changes at Cordyceps fermentation broth during fermentation were examined by HPLC analysis. The Cordycepin concentration found in the fermentation broth containing skim milk or sucrose was ranged from 0.05 to 1.75 mg /mL or from 0.16 to 0.79 mg / mL, respectively. All fermentation broths containing skim milk and sucrose have shown to contain a significant amount of adenosine. The Adenosine concentration found in the fermentation broth containing skim milk or sucrose was ranged from 0.48 to 4.86 mg /mL or from 1.41 to 4.82 mg / mL, respectively. There are also significant amount of tea polyphenols distribution found in all fermentation broth by HPLC analysis. Based on the aforementioned information, the Cordyceps fungi cultured in green tea liquid containing sucrose can promote the growth number of Cordyceps fungi. Its fermentation broth has shown good functional ingredients and ability, and can be potentially an excellent health drink.
中文摘要...............................................................................................................I
英文摘要............................................................................................................III
誌謝……………………………………………………………………………IV
目錄.....................................................................................................................V
圖目錄................................................................................................................IX
表目錄................................................................................................................XI
第一章 緒論
1-1前言.................................................................................................1
第二章 文獻回顧
2-1北蟲草......................................................................................3
2-2北蟲草的藥理功能..................................................................4
2-2-1延緩衰老、抗疲勞和耐缺氧.........................................4
2-2-2提高人體免疫功能........................................................4
2-2-3抗癌作用........................................................................5
2-2-4抗菌及抗病毒作用........................................................6
2-2-5驚厥及鎮靜作用............................................................6
2-2-6促使雄性激素分泌作用................................................7
2-2-7對心腦血管系統疾病的作用........................................7
2-2-8糖尿病治療....................................................................7
2-2-9抗氧化與清除自由基....................................................7
2-3北蟲草主要成分與營養價值..................................................9
2-3-1北蟲草機能性成份........................................................10
2-3-1-1核苷類(Nucleoside).......................................................10.
2-3-1-2蟲草素(Cordycepin)...............................................12
2-3-1-3蟲草多醣體(Cordycepic polysaccharide)...............13
2-3-1-4蛹蟲草胺基酸組成(Amino acid)...........................13
2-3-1-5超氧化物歧化酶(SOD)........................................14
第三章 實驗材料與分析方法
3-1實驗儀器................................................................................15
3-2實驗藥品................................................................................16
3-3北蟲草菌活化........................................................................18
3-4北蟲草菌培養於綠茶發酵液................................................19
3-5北蟲草菌於發酵液生長與生長數目評估方法....................20
3-6發酵液功能性分析................................................................22
3-7 DPPH自由基清除能力測定...........................................23
3-7-1原理...........................................................................23
3-7-2標準品校正線製作...................................................24
3-7-3樣品測定...................................................................24
3-8總多酚含量測定....................................................................26
3-8-1原理...........................................................................26
3-8-2標準品校正線製作...................................................26
3-8-3樣品測定...................................................................27
3-9總醣量測定............................................................................29
3-9-1原理...........................................................................29
3-9-2標準品校正線製作...................................................29
3-9-3樣品測定...................................................................29
3-10 HPLC分析蟲草素、腺苷與分析條件......................................31
3-11統計分析..............................................................................32
第四章 結果與討論
4-1北蟲草於添加脫脂乳發酵液之生長................................33
4-2北蟲草於添加蔗糖綠茶發酵液之生長....................................35
4-3 DPPH自由基清除能力.........................................................37
4-3-1脫脂乳綠茶發酵液之DPPH自由基清除能力...37
4-3-2蔗糖綠茶發酵液之DPPH自由基清除能力…...39
4-4總多酚含量............................................................................41
4-4-1脫脂乳綠茶發酵液之總多酚含量………….………...41
4-4-2蔗糖綠茶發酵液之總多酚含量………………………...44
4-5總醣含量................................................................................47
4-5-1脫脂乳綠茶發酵液之總醣含量……...……..…………...47
4-5-2蔗糖綠茶發酵液之總醣含量……………………...……50
4-6 HPLC分析核苷酸.................................................................53
4-6-1添加脫脂乳綠茶發酵液之核苷酸分析...................53
4-6-2添加蔗糖綠茶發酵液之核苷酸分析......................58
4-6-3綠茶發酵液之蟲草素定量分析.........................................63
4-6-4綠茶發酵液之腺苷定量分析.............................................68
4-7 HPLC分析茶多酚.................................................................73
4-7-1含碳源北蟲草發酵液茶多酚HPLC分析………………73
4-7-2含氮源北蟲草發酵液茶多酚HPLC分析………………74
第五章 結論............................................................................................75
第六章 參考文獻....................................................................................77

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