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研究生:張富傑
研究生(外文):Chang,Fu-Chieh
論文名稱:天然物萃取液之光電性質研究
論文名稱(外文):Studies on the Electro-Optical Properties for Natrual Products Extracts
指導教授:黃清江黃清江引用關係
指導教授(外文):Ching-Chiang Hwang
口試委員:徐力行黃英邦黃清江
口試委員(外文):Shyu,Lih-ShyngHuang,Lng-BangHwang,Ching-Chiang
口試日期:2012-07-19
學位類別:碩士
校院名稱:明道大學
系所名稱:光電暨能源工程學系碩士班
學門:工程學門
學類:綜合工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:70
中文關鍵詞:循環伏安法還原力銀杏葉山楂酪胺酸酶
外文關鍵詞:Cyclic volammetryReducing powerHawthornGinkgoTyrosinase
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摘要
本研究目的是利用天然物銀杏葉和山楂的萃取液來做電化學性質探討和美白能力及還原能力探討。利用循環伏安法實驗來分析天然物電化學性質。循環伏安實驗中,分別用銀杏葉和山楂萃取液來來做為電池電解液,實驗中發現這兩種天然物在SS 304不鏽鋼極板上,都會產生鈍化膜,這是具有陰極抗腐蝕的效果,所以有開發天然腐蝕抑制劑的空間。另外一方面以銀杏葉和山楂萃取液來對酪胺酸酶的抑制效果作為美白效果活性實驗,由於酪胺酸酶為形成黑色素時之關鍵酵素,因此酪胺酸酶抑制劑常被視為有皮膚美白作用,實驗中了解到這兩種天然物萃取液皆有抑制酪胺酸酶的效果,酵素動力學的實驗顯示,銀杏葉是非競爭型抑制劑,山楂是類似競爭型的抑制效果。實驗中可發現當受質濃度增加也無法克服銀杏葉當抑制劑的抑制效果,卻可以克服山楂當抑制劑的抑制效果。以這兩種萃取液做還原力效果實驗,結果顯示在低濃度當山楂取液的濃度2 ml/l增加至5 ml/l時,還原力明顯會上升,且在濃度2 ml/l增加至5 ml/l時還原力不明顯,但是在高濃度10 ml/l增加至100 ml/l時,這兩種萃取液還原力都明顯上升,但是山楂的還原力仍優於銀杏葉。
關鍵詞:酪胺酸酶,山楂,銀杏葉,循環伏安法,還原力。

Abstract
The purpose of this research is to make use of natural gingko leaves and the extracted liquid of hawthorn to engage in an exploration in relation to the feature of electrochemistry, as well as to the capacity of whitening and Reducing poewr. The experiment of Cyclic voltammetry will be used to analyze the electrochemistry attribute of natural substance. In the course of the experiment of Cyclic voltammetry, the extracted liquid derived from both Ginkgo and hawthorn is to be used as the electrolyte of battery. The finding in the course of experiment was that these two natural substances are to generate the blunt membrane on the cell plate of stainless steel called SS 304. This is encompasses the effect of negative-pole anti-erosion. Therefore, this results in the room of exploiting natural inhibitor against erosion. On the other hand, the extracted liquid of Ginkgo and hawthorn is used, by means of its inhibiting effect upon Tyrosinase, to engage in the active experiment of whitening effect. Due to its role as a crucial enzyme when the Melanin is formed, the inhibitor of Tyrosinase has often been viewed as having the whitening effect. It has been recognized that in the course of experiment, these two natural extracted liquids are in possession of the effect of inhibiting Tyrosinase. Further, the experiment of Enzyme Kinetics shows that Gingko is non-competitive inhibitor, and that hawthorn has its effect similar to that of competitive inhibitor. It has been found during the experiment that when substrate concentration increases, it is impossible to overcome the inhibiting effect caused by Ginkgo, but possible to overcome the inhibiting effect caused by Hawthorn. When we use these two extracted liquids to engage in the capacity of Reducing poewr experiment, the result shows that on the occasion of low concentration, with the low concentration of Hawthorn of 2ml/l increased to 5ml/l, the capacity of Reducing poewr will by far increase. Also, when the concentration has been diluted from 2ml/l to 5ml/l, the capacity of Reducing poewr is not prominent. However, on the occasion of high concentration, with 10ml/l increased to 100ml/l, there will be a prominent increase for these two extracted liquids. But the capacity of Reducing poewr for Hawthorn is superior to that of Ginkgo.
Key Words: Tyrosinase,Ginkgo,Hawthorn,Reducing power,Cyclic volammetry

目 錄
摘要 .................................................................................................... i
Abstract .............................................................................................. ii
目錄 .................................................................................................... iv
圖目錄 ................................................................................................ vi
表目錄 ................................................................................................ viii
第一章 前言 .................................................................................... 1
第二章 原理及文獻回顧 ................................................................ 4
2.1銀杏葉 ....................................................................................... 4
2.1.1銀杏葉效用 ............................................................................ 4
2.1.2山楂 ........................................................................................ 5
2.1.3山楂效用 ................................................................................ 6
2.2循環伏安 ................................................................................... 7
2.3氧化還原 ................................................................................... 9
2.4美白能力探討 ........................................................................... 11
2.4.1人體皮膚簡介 ........................................................................ 11
2.4.2酪胺酸酶 …............................................................................ 13
2.4.3黑色素形成 ............................................................................ 14
2.4.4常見美白方法 ….................................................................... 21
第三章 實驗材料與方法 …............................................................ 25
3.1實驗架構圖 ............................................................................... 25
3.2實驗用品 ................................................................................... 25
3.2.1實驗儀器 ................................................................................ 25
3.2.2天然物來源 ............................................................................ 26
3.2.3實驗藥劑 ................................................................................ 26
3.3實驗步驟 ……........................................................................... 27
3.3.1山楂、銀杏葉萃取方法........................................................ 27
3.3.2電化學實驗.............................................................................. 29
3.3.3 天然物還原能力實驗 .......................................................... 31
3.3.4美白能力測定 ….................................................................... 33
第四章 結果與討論 ........................................................................ 35
4.1電化學性質分析 ....................................................................... 35
4.1.1山楂萃取液 …........................................................................ 35
4.1.2銀杏葉萃取液 ….................................................................... 43
4.2還原力實驗 …........................................................................... 51
4.3美白能力實驗 …....................................................................... 54
4.3.1酵素動力學實驗 …................................................................ 54
第五章 結論 ………….................................................................... 64
第六章 參考文獻 …........................................................................ 66

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