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研究生:阮氏明舒
研究生(外文):Minh-Thu ThiNguyen
論文名稱:發展氧化鋅奈米粒子以改善牙科填補材的抗菌與機械性質
論文名稱(外文):Development of ZnO Nanoparticles for Improvement of Antibacterial and Mechanical Properties of Dental Luting Cement
指導教授:謝達斌
指導教授(外文):Dar-Bin Shieh
學位類別:碩士
校院名稱:國立成功大學
系所名稱:口腔醫學研究所
學門:醫藥衛生學門
學類:牙醫學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:英文
論文頁數:76
中文關鍵詞:氧化鋅奈米粒子抗菌性機械性質牙科填補材料
外文關鍵詞:zinc oxide nanoparticleantibacterial propertymechanical propertyluting cement
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金屬氧化物奈米粒子為一被廣泛使用於各種領域的新穎材料,包含生醫影像、藥物傳輸及其它健康照護相關領域。在牙醫學中,氧化鋅塊材由於具有優秀的機械性質與生物相容性,一直被用來當作暫時或永久性填補材料主成分。其中,磷酸鋅填補材料具有良好的抗壓強度,而聚羧酸鋅填補材料可與礦物組織表面產生化學鍵結。藉由奈米科技的改善,具有不同晶格結構與構型的氧化鋅奈米粒子已被成功製備。獨特性氧化鋅奈米粒子相較於相同重量的塊材擁有更高的表面積與較小的尺寸。因此,氧化鋅奈米粒子具更好的抗菌性以及當成填充物,來增強密度與減少材料的空洞,而得以取代現今填補材料中的部分氧化鋅塊材,從而改善填補材料的整體性質。本論文致力於改善填補材料的抗菌性,以作為避免牙科填補材料損耗的解決方案;尤其是二次齲齒與口腔細菌(活動)所造成的牙周病所導致的補綴填補物與牙齒結構間之失敗。當多於10%合成的氧化鋅奈米粒子取代聚羧酸鋅填補材料中的氧化鋅塊材後,抗菌性有顯著提升。此外,含氧化鋅奈米粒子填補材料的機械性質較傳統的氧化鋅塊材填補材料佳。總結,在填補材料中奈米氧化鋅的添加能在牙科復形上對二次齲齒與牙周病的預防提供一解決方案之發展潛力。本研究的發現將可同時助益於進一步改善牙科填補材料的內在的抗菌性與更好機械性質。
Metal oxide nanoparticles are known as a new class of important materials that have been implemented for various applications such as biomedical imaging, drug delivery, and other healthcare related fields. In dentistry, bulk zinc oxide particles have been used for temporal or permanent luting cement due to their excellent mechanical strength and biocompatibility. Among these, zinc phosphate cement exhibits excellent compressive strength, while the zinc polycarboxylate cement presents chemical bonding to tooth mineral surfaces. With the advancement of nanotechnology, zinc oxide nanoparticles with different crystal structures and conformation have been synthesized. The zinc oxide nanoparticles possess smaller size and higher surface area compared with their bulk counterparts. As a result, the zinc oxide nanoparticles are more active in antibacterial activity, and they act as fillers to enhance the density and to reduce the porosity of the zinc oxide contained materials. These properties might have certain niches to use zinc oxide nanoparticles to replace a part of the bulk zinc oxide particles of current cements, and thus it improves the overall properties of the cements. In the present study, it was aimed to improve the antibacterial property of luting cement as a solution for preventing the failure of dental restoration, the development of secondary caries and (or) periodontitis in the tooth-prosthetic junction when attacked by cariogenic bacteria. It was discovered that antibacterial activity was significantly improved when more than 10% of the synthesized zinc oxide nanoparticles were used to replace a part of the bulk particles of conventional zinc oxide polycarboxylate cement. In addition, mechanical properties of zinc oxide nanoparticles contained cement were better than that of conventional zinc oxide polycarboxylate cement. In conclusion, this study presented a new dental luting cement system with integrated nano-ZnO that provides a promising solution for potential prevention of secondary carries and periodontitis. The discovery of this study could simultaneously contribute to the concept for future improvement of dental restorative cement with build in antibacterial and better mechanical properties.
TABLE OF CONTENTS

ENGLISH ABSTRACT 1
CHINESE ABSTRACT 3
ACKNOWLEDGEMENT 4
LIST OF TABLES 7
LIST OF FIGURES 8
CHAPTER
1 INTRODUCTIION
1.1 Zinc polycarboxylate luting cement 13
1.2 Cast restoration 14
1.3 Activities of zinc oxide nanoparticles (ZnO NPs) 15
2 MATERIALS AND METHODS
2.1 Synthesis of ZnO NPs 17
2.2 Test of antibacterial activity 18
2.3 Test of compressive strengths 19
2.4 Test of diametral tensile strengths 20
2.5 Test of shear bond strengths 20
2.6 Test of solubility 21
2.7 Test of biocompatibility 22
2.7.1 Cells treated directly with cement disks 23
2.7.2 Cells treated with liquid extracted from cement disks 23
2.7.3 Morphology of cells around cement disks 24
3 RESULTS
3.1 Characterization of synthesized ZnO NPs 25
3.2 Test of antibacterial activity 25
3.3 Test of compressive strengths 26
3.4 Test of diametral tensile strengths 26
3.5 Test of shear bond strengths 27
3.6 Test of solubility 27
3.7 Test of biocompatibility
3.7.1 Cells treated directly with cement disks 28
3.7.2 Cells treated with liquid extracted from cement disks 28
3.7.3 Morphology of cells around cement disks 29
4 DISCUSSION 30
4.1 Antibacterial property 30
4.2 Mechanical properties 33
4.3 Adhesive strength 34
4.4 Solubility 36
4.5 Biocompatibility 38
5 CONCLISION and RECOMMENDATIONS 40
REFERENCES 42
CURRICULUM VITA 76

Abo-Hamar SE, Hiller K-A, Jung H, Federlin M, Friedl K-H , Schmalz G. Bond strength of a new universal self-adhesive resin luting cement to dentin and enamel. Clinical Oral Investigations 9 (3) 161-167 (2002).

Adams LK, Lyon DY, Alvarez PJJ. Comparative eco-toxicity of nanoscale TiO2, SiO2, and ZnO water suspensions. Water Research 40 (19): 3527-3532 (2006).

Arefi and Rezaei-Zarchi. Synthesis of Zinc Oxide Nanoparticles and Their Effect on the Compressive Strength and Setting Time of Self-Compacted Concrete Paste as Cementitious Composites. International Journal of Molecular Sciences 13 (4): 4340-4350 (2012).

Beech DR. A spectroscopic study of the interaction between human tooth enamel and polyacrylic acid (polycarboxylate cement). Archives of Oral Biology 17 (5): 907-911 (1972).

Brauer DS, Gentleman E, Farrar DF, Stevens MM , Hill RG. Benefits and drawbacks of zinc in glass ionomer bone cements. Biomedical Materials 6 (4): (2011).

Bresciani E, Barata TDJE, Fagundes TC, Adachi A, Terrin MM , Navarro MFDL. Compressive and diametral tensile strength of glass ionomer cements. Journal of Applied Oral Science 12: 344-348 (2004).

Burnett ME, Wang SQ. Current sunscreen controversies: a critical Review. Photodermatology, Photoimmunology & Photomedicine 27 (2): 58-67 (2011).

Causton BE, Johnson NW. Changes in the dentine of human teeth following extraction and their implication for in-vitro studies of adhesion to tooth substance. Archives of Oral Biology 24 (3): 229-232 (1979).

Certification programs of the Council on Dental Materials, Instruments and Equipment. Chicago, Ill: American National Standards Institution/ American Dental Association specification 61 for zinc polycarboxylate cement. 1977.

Chang H-J, Wu C-M, Chang Y-C, Fanchiang J-C, Shieh D-B , Wong T-Y Collagen Enhances Compatibility and Strength of Glass Ionomers. Journal of Dental Research 88 (5): 449-454 (2009).

Chang Y-Y, Lai C-H, Hsu J- T, Tang C-H, Liao W-C , Huang H-L. Antibacterial properties and human gingival fibroblast cell compatibility of TiO2/Ag compound coatings and ZnO films on titanium-based material. Clinical Oral Investigations 16 (1): 95-100.

Chen IC, Liou Y-CM, Yang J , Shieh T-Y. Preparation of silver nanoparticles on zinc oxide nanowires by photocatalytic reduction for use in surface-enhanced Raman scattering measurements. Journal of Raman Spectroscopy 42 (3): 339-344 (2011).

Colon G, Ward BC , Webster TJ. Increased osteoblast and decreased Staphylococcus epidermidis functions on nanophase ZnO and TiO2. Journal of Biomedical Materials Research Part A 78A (3): 595-604 (2006).

Duymus ZY. An investigation of pH changes of various cement. Quintessence international (Berlin, Germany:1985) 35 (9): 753-757 (2004)

Fang M, Chen JH, Xu XL, Yang PH. Antibacterial activities of inorganic agents on six bacteria associated with oral infections by two susceptibility tests. International Journal of Antimicrobial Agents 27 (6): 513-517 (2006).

Hardie JM. Oral microbiology: current concepts in the microbiology of dental caries and periodontal disease. British Dental Journal 172 (7): 271-278 (1992).

Iwaku M, Takatsu T, Fusayama T. Comparison of three luting agents. The Journal of Prosthetic Dentistry 43 (4): 423-425 (1980).

Jacobs MS, Windeler AS. An Investigation of Dental Luting Cement Solubility as a Function of the Marginal Gap. The Journal of Prosthetic Dentistry 65 (3): 436- 442 (1991).

Jahangiri L, Wahlers C, Hittelman E , Matheson P. Assessment of sensitivity and specificity of clinical evaluation of cast restoration marginal accuracy compared to stereomicroscopy. Journal of Prosthetic Dentistry 93 (2): 138-142 (2005).

Jiang W, Mashayekhi H, Xing B. Bacterial toxicity comparison between nano- and micro-scaled oxide particle. Environmental Pollution 157 (5): 1619-1625 (2009).

Jones N, Ray B, Ranjit KT, Manna AC. Antibacterial activity of ZnO Nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiology Letter 279 (1): 71-76 (2008).

Kan KC, Messer LB, Messer HH. Variability in Cytotoxicity and Fluoride Release of Resin-modified Glass-ionomerCements. Journal of Dental Research 76 (8): 1502-1507 (1997).

Karlsson HL, Cronholm P, Gustafsson J, Möller L. Copper Oxide Nanoparticles Are Highly Toxic: A Comparison between Metal Oxide Nanoparticles and Carbon Nanotubes. Chemical Research in Toxicology 21 (9): 1726-1732 (2008).

Kendzior GM, Leinfelder KF, Garland Hershey H. The Effect of Cold Temperature Mixing on the Properties of Zinc Phosphate Cement. The Angle Orthodontist 46 (4): 345-350 (1976).

Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, Kim SH, Park YK, Park YH, Hwang CY, Kim YK, Lee YS, Jeong DH, Cho MH. Antimicrobial effects of silver nanoparticles. Nanomedicine: Nanotechnology, Biology, and Medicine 3 (1): 95-101 (2007).

Kois JC. The restorative- periodontal interface: biological parameters. Periodontology 2000 11 (1): 29-38 (1996).

Levine MJ, Aguirre A, Hatton MN, Tabak LA. Artificial salivas: present and future. Journal of Dental Research 66: 693-698 (1987).

Leyhausen G, AbtahiM, Karbakhsch M, Sapotnick A , Geurtsen W. Biocompatibility of various light-curing and one conventional glass ionomer cement. Biomaterials 19 (6): 559-564 (1998).

Lia ZC, White SN. Mechanical properties of dental luting cements. The Journal of Prosthetic Dentistry 81 (5): 597-609 (1999).

Ling-Min Y, Xin-Hui F, LI-Jun Q , Wen Y. Field emission property of printed CNTs-mixed ZnO nanoneedles. Applied Surface Science 257 (15): 6332-6335 (2011).

Lipovsky A, Nitzan Y, Gedanken A, Lubart R. Antifungal activity of ZnO nanoparticles—the role of ROS mediated cell injury. Nanotechnology 22 (10): 105101 (2011).

Nagarajan P, Rajagopalan V. Enhanced bioactivity of ZnO nanopartcles—an antimicrobial study. Science and Technology of Advanced Materials 9 (3): 035004 (2008).

Nakabayashi N, Kojima K, Masuhara E. The promotion of adhesion by the infiltration of monomers into tooth substrates. Journal of biomedical materials research 16 (3): 265-273 (1982).

Newman MD, Stotland M, Ellis JI. The safety of nanosized particles in titanium dioxide- and zinc oxide-based sunscreens. Journal of the American Academy of Dermatology 61 (4): 685-692 (2009).

Oliva A, Dellaragione FD, Salerno A, Riccio V, Tartaro G, Cozzolino A, Damato S, Pontoni G, Zappia V. Biocompatibility studies on glass ionomer cements by primary cultures of human osteoblasts. Biomaterials 17 (13): 1351-1356 (1996).

Osborne JW, Wolff MS. The effect of powder/liquid ratio on the in vivo solubility of polycarboxylate cement. The Journal of Prosthetic Dentistry 66 (1): 49-51(1991).

Plum LJ, Arends J, Havinga P, Jongebloed WL, Stokroos I.Quantitative cement solubility experiments in vivo. Journal of Oral Rehabilitation 11 (2): 171-179 (1984).

Øilo G, Espevik S.Stress/strain behavior of some dental luting cements. Acta Odontologica Scandinavica 36 (1): 45-49 (1978).

Riahi S,NazariA.Physical,mechanical and thermal properties of concrete in different curing media containing ZnO2 nanoparticles. Energy and Buildings 43 (8): 1977-1984 (2011).

Sawai J. Quantitative evaluation of antibacterial activities of metallic oxide powders (ZnO, MgO and CaO) by conductimetric assay. Journal of Microbiological Methods 54 (2): 177-182 (2003).

Schilling K, Bradford B, Castelli D, Dufour E, Nash JF, Pape W, Schlte S, Tooley I,Van Den BoschJ, Schellauf F. Human safety review of nano titanium dioxide and zinc oxide. Photochemical & Photobiological Sciences 9 (4): 495-509 (2010).

Smith DC. A new dental cement. British dental journal 124 (9): 381-384 (1968).

Smith DC. Medical and dental applications of cements. Journal of biomedical materials research 5 (2): 189-205 (1971).

Tang X, Zhang B,Xie G, Xia X. Study on antibacterial mechanism of Ag-inorganic antibacterial material containing lanthanum. Advanced Materials Research 79-82: 1799-1802 (2009).

Tuntiprawon M, Wilson PR. The effect of cement thickness on the fracture strength of all-ceramic crowns. Australian Dental Journal 40 (1): 17-21(1995).

Wang RC, Lin HY, Chen SJ, Lai YF , Huang MRS. Boundary layer- assisted chemical bath deposition of well-aligned ZnO rods on Si by a one-step method. Applied Physics A: Materials Science & Processing 96 (3): 775-781 (2009).

Wei J, Wang J, Shan W, Liu X, Ma J, Liu C, Fang J, Wei S. Development of fluorapatite cement for dental enamel defects repair. Journal of Materials Science: Materials in Medicine 22 (6): 1607-1614 (2011).

White SN , Yu Z. Compressive and diametral tensile strengths of current adhesive luting agents. Journal of Prosthetic Dentistry 69 (6): 568-572 (1993).

Wilson AD.The chemistry of dental cements .Chemical Society Reviews 7 (2): 265-296 (1978).

Wilson AD, Paddon JM, Crisp S. The Hydration of Dental Cements. Journal of Dental Research 58 (3): 1065-1071 (1979).

Xie Y, He Y, Irwin PL, Jin T, Shi X. Antibacterial Activity and Mechanism of Action of Zinc Oxide Nanoparticles against Campylobacter Jejuni. Applied and Environmental Microbiology 77 (7): 2325-2331(2011).

Ximénez-fyvie LA, Haffajee AD, Saransk SS. Comparison of the microbiota of supra- and subgingival plaque in health and periodontitis. Journal of Clinical Periodontology 2000; 27 (9): 648-657.

Yanikoglu N, Yesil Duymus Z. Evaluation of the Solubility of Dental Cements in Artificial Saliva of Different pH Values. Dental Materials Journal 26 (1): 62-67 (2006)

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