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研究生:郭榮哲
研究生(外文):Sugihatta HendrataKuswono
論文名稱:利用雙吸收塔輔助式光反應器提升小球藻產量之研究
論文名稱(外文):Improving Biomass Production of Chlorella sp Cultivation by Implementing A Double-Bubble-Column Photo-bioreactor
指導教授:吳文騰
指導教授(外文):Wen-Teng Wu
學位類別:碩士
校院名稱:國立成功大學
系所名稱:化學工程學系碩博士班
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:英文
論文頁數:89
中文關鍵詞:小球藻光生物反應器混合逆流配置生質產量
外文關鍵詞:Chlorella sp.photo-bioreactormixing treatmentcounter-current flow patternbiomass production
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開放式微藻培養池目前最主要困難在於無法穩定維持最佳培養環境,以至於生物質產量始終無法達到預期。為了提高二氧化碳的使用效率,本研究設計了新型培養槽,利用兩個各1L的吸收塔供應40L主培養槽之碳源,藉由自吸收塔底端混合通入1 vvm (1%CO2)的空氣,以提高培養槽中曝氣效率。此外,量測主培養槽內導電度,藉以評估其攪拌混合程度是否良好。為了瞭解流動模式對微藻產量之影響,本研究進一步探討順、逆流配置的差異。逆流搭配沉水幫浦可提供最短混合時間,因此本研究採用此一設計。
研究成果顯示,於培養體積為42 L光生化反應器進行九天週期性光照批次培養(初始濃度為0.1g/L)中,雙吸收塔設計優於單吸收塔,其生質濃度分別為1.011 g/L及0.900 g/L;對應之生質總產量為42.47 g及37.81 g。另外為了提高產量,本研究進一步採用重複批次培養。於相同條件下,自開始培養起始後4.5天(停滯期前)以20%的置換比例進行,將培養體積提升至84 L,可獲得最終生質濃度為0.593 g/L ,總生質產量達60.14 g。
由以上結果得知,此研究所使用之光生化反應器結合重複批次培養策略可有效提升生質產量。



Microalgae cultivated in open pond system have several difficulties to maintain optimum conditions for its growth, as a result biomass production is always lower than the expected amount. For increasing CO2 uptake, absorption tower was developed to supply CO2 into a photo-bioreactor. A proposed photo-bioreactor cultivation system consisted of a 42 L rectangular open tank equipped with 2-bubble columns of 1 L separately. From the bottom of each bubble column, air aeration was given at the flow rate of 1 vvm (1% CO2). Mixing performance inside the whole bioreactor had been examined through measuring the electric conductivity of water-salt solution taken from the tank. The flow pattern of the culture system was investigated with co-current and counter-current configurations. Counter-current flow pattern with external mixing treatment provided the shortest mixing time. Thus, we adopted counter-current configuration in our photo-bioreactor cultivation system.
In this study, Chlorella sp. were cultivated for 9 days. The reactor and working volume used in the batch cultivation were 42 L. Higher biomass production was obtained when using 2-bubble columns in comparison with 1-bubble column, there were 1.011 g/L and 0.900 g/L, respectively and calculated as an absolute biomass at 42.47 g and 37.81 g Then, in order to increase absolute biomass production, fed-batch strategy in which daily renewal rate is 20% since 4.5 day (the early stage of the stationary phase) was applied and the working volume increased to 84 L. Biomass production obtained is 0.593 g/L and the absolute biomass rose to 60.14 g. These results indicate that our proposed photo-bioreactor combined with the fed-batch cultivation can successfully enhance biomass production.

ABSTRACT I
摘要 II
ACKNOWLEDGEMENTS III
TABLE OF CONTENTS IV
LIST OF TABLES VII
LIST OF FIGURES VIII
CHAPTER 1 INTRODUCTION 1
1.1. Research Background. 1
1.2. Objectives. 5
1.3. Thesis structure. 6
CHAPTER 2 LITERATURE REVIEW 7
2.1. Introduction to Algae. 7
2.2. Physiology of microalgae. 9
2.2.1. Photosynthesis in algae. 9
2.2.2. Lipid biosynthesis in microalgae. 12
2.2.3. Environmental effects on cell growth. 15
2.3. Microalgae Cultivation. 23
2.3.1. Type of growth. 24
2.3.2.Cultivation system. 26
CHAPTER 3 RESEARCH DESIGN AND METHODOLOGY 33
3.1. Seed of Microalgae. 33
3.2. Composition of Medium. 33
3.3. Experimental Instrument. 36
3.3.1. Cultivation system. 36
3.3.2. Analytical instruments. 37
3.4. Photo-bioreactor. 37
3.4.1. Evaluation of reactor mixing performance. 38
3.4.2. Photo-bioreactor equipped with single bubble column. 39
3.4.3. The proposed photo-bioreactor equipped with double bubble column. 40
3.6. Method of Cultivation. 42
3.6.1 Algae seed storage. 42
3.6.2 Pre Culture and Scale up Culture. 42
3.6.3. Main Cultivation. 42
3.6.4. Experiment Flowchart. 43
3.7. Measurement and analytical methods. 43
3.7.1. Light Intensity. 43
3.7.2. Analysis of biomass concentration 43
3.7.3. Urea concentration analysis. 45
3.7.4. Lipid Analysis. 46
CHAPTER 4 RESULTS AND DISCUSSION 49
4.1. Photo-bioreactor design. 49
4.1.1. Volume ratio of the bubble column to the open tank. 49
4.1.2. Different flow pattern configuration. 50
4.1.3. Light arrangement. 50
4.1.4. External mixing by using submerged pumps. 51
4.1.5. Mixing performance evaluation. 52
4.2. Batch cultivation. 64
4.2.1. Biomass concentration of photo-bioreactor equipped with single bubble column 64
4.2.2. Biomass concentration of photo-bioreactor equipped with double bubble columns and co-current flow configuration. 66
4.2.3. Comparison of biomass concentration between photo-bioreactor equipped with single and double bubble columns (co-current flow configuration). 68
4.2.4. Comparison of biomass concentration of photo-bioreactor equipped with double bubble-columns between co- and counter-current flow configuration 70
4.3. Fed batch cultivation. 72
4.4. Summaries of absolute biomass production and reactor performance at different photo-bioreactor and cultivation strategies. 74
4.5. Lipid content, carbon utilization efficiency and photosynthetic efficiency. 76
CHAPTER 5 CONCLUSION AND SUGGESTION 78
5.1. Conclusion 78
5.2. Suggestion. 78
REFERENCES 80

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