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研究生:張維芬
研究生(外文):Wei-FenChang
論文名稱:添加劑對雙十六碳鏈離子對雙親分子形成之陰陽離子液胞物化特性的影響
論文名稱(外文):The Effects of Additives on the Physicochemical Properties of Catanionic Vesicles Formed by Dihexdecyl-Chained Ion Pair Amphiphile
指導教授:張鑑祥張鑑祥引用關係
指導教授(外文):Chien-Hsiang Chang
學位類別:碩士
校院名稱:國立成功大學
系所名稱:化學工程學系碩博士班
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:106
中文關鍵詞:陰陽離子液胞離子對雙親分子液胞液胞雙層膜
外文關鍵詞:catanionic vesicleion pair amphiphilevesiclevesicular bilayer
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  • 被引用被引用:6
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本研究藉由提高溫度增加離子型界面活性劑在水中的溶解度,並利用沉澱方法製備出雙十六碳鏈離子對雙親分子(ion pair amphiphile, IPA)hexadecyltrimethylammonium-hexadecylsulfate (HTMA-HS)。然後添加不同莫耳分率的膽固醇及雙十六碳磷酸鹽(dihexadecyl phosphate, DHDP),以強制性製程由HTMA-HS製備出陰陽離子液胞 (catanionic vesicle),並探討添加劑對HTMA-HS所形成之液胞物化性質的影響。此外,與利用其他兩種碳鏈長度不同之IPA所製備出的陰陽離子液胞比較,分析IPA碳鏈長度及對稱性對陰陽離子液胞物化性質的影響。
實驗結果顯示,利用HTMA-HS可製備出穩定達50天的陰陽離子液胞,但液胞間有明顯的聚集。添加適量的膽固醇或DHDP可減緩液胞間聚集或融合的速率,但可能使液胞雙層膜內的結構產生缺陷,因而對液胞物理穩定性的提升效果有限。藉由同時添加膽固醇和DHDP,可增強液胞雙層膜的機械強度,並增加液胞間的靜電排斥作用,使得液胞的物理穩定性有顯著的改善。以紅外光光譜法分析液胞雙層膜內分子碳氫鏈的排列,發現在HTMA-HS雙層膜結構之相轉移溫度下添加膽固醇,會使分子碳氫鏈的排列變得較不規則,DHDP的添加則只會略微降低分子碳氫鏈排列的規則度。
分析不同IPA添加膽固醇後所形成之陰陽離子液胞的物化特性,發現IPA碳鏈長度越長,所形成之液胞的界面電位越大,此現象可能是HTMA-HS所形成之液胞比其他兩種IPA所形成之液胞有較佳物理穩定性的原因。然而碳鏈較短且對稱之IPA (DTMA-DS)的疏水碳鏈與膽固醇的作用較佳,並有良好之結構上的配位,因此添加少量的膽固醇即可明顯提升其液胞之物理穩定性。

In this study, the surfactant solubility in water was increased by rasimg the temperature and the ion pair amphiphile (IPA) hexadecyltrimethylammonium-hexadecylsulfate (HTMA-HS) was prepared by a precipitation approach. Then the catanionic vesicles were fabricated from HTMA-HS with the addition of different molar fractions of cholesterol and dihexadecyl phosphate (DHDP) by a force formation process, and the effects of additives on the physicochemical properties of the catanionic vesicles composed of HTMA-HS were investigated. In addition, the effects of alkyl chain length and symmetry of IPA on the physicochemical properties of catanionic vesicles were analyzed by comparing the catanionic vesicles composed of HTMA-HS with those composed of two other IPAs with different chain lengths.
  The experimental results indicated that catanionic vesicles which were stable up to 50 days could be prepared from HTMA-HS. However, significant aggregation between the vesicles was detected. The addition of a proper amount of cholesterol or DHDP could slow down the aggregation or fusion rates of the vesicles, but defects in the vesicular bilayer structures might be induced, resulting in limited enhancement effect on the physical stability of the catanionic vesicles. By adding both cholesterol and DHDP, the mechanical strength of the vesicular bilayers and the electrostatic repulsion between the vesicles were increased, and the physical stability of the catanionic vesicles could be significantly improved. The arrangement of molecular alkyl chains in the vesicular bilayers was analyzed by the infrared spectroscopy, and it was found that the molecular alkyl chains became less ordered when cholesterol was added below the phase transition temperature of the HTMA-HS bilayers. The addition of DHDP only slightly lowered the order of the molecular alkyl chains.
  By analyzing the physicochemical characteristics of the catanionic vesicles composed of different IPAs with the addition of cholesterol, one could find that the longer the alkyl chain length of IPA, the more positive the zeta potential of catanionic vesicles. This might be the reason for the vesicles composed of HTMA-HS possessing better physical stability than the vesicles composed of two other IPAs. However, the IPA (DTMA-DS) with shorter and symmetric alkyl chains could better interact and match well in structure with cholesterol. As a result, adding a small amount of cholesterol could significantly enhance the physical stability of the vesicles composed of DTMA-DS.

摘要 I
Abstract III
誌謝 V
目錄 VI
表目錄 IX
圖目錄 XI
符號說明 XV
第一章 緒論 1
1-1 前言 1
1-2 研究動機與目的 3
1-3 文獻回顧 3
1-3-1 離子對雙親分子 3
1-3-2 帶電液胞 5
1-3-3 膽固醇的效應 6
1-3-4 液胞雙層膜中分子碳氫鏈的排列 8
1-3-5 陰陽離子界面活性劑之碳鏈對稱性和長度的效應 9
第二章 實驗 20
2-1 藥品 20
2-2 實驗儀器及裝置 20
2-2-1 超音波震盪分散裝置 20
2-2-2 雷射光散射法粒徑及界面電位分析儀 21
2-2-3 元素分析儀 24
2-2-4 傅利葉轉換紅外光譜儀 25
2-2-5 穿透式電子顯微鏡 26
2-3 實驗方法 27
2-3-1 HTMA-HS的製備 27
2-3-2 液胞分散液的製備 28
2-3-3 粒徑分布及界面電位的量測 29
2-3-4 穿透式紅外光光譜的分析 29
第三章 結果與討論 39
3-1 離子對雙親分子 39
3-2 陰陽離子液胞的合成 41
3-2-1 HTMA-HS形成之陰陽離子液胞 41
3-2-2 帶負電之陰陽離子液胞 42
3-2-3 膽固醇的影響 44
3-2-4 含膽固醇之帶負電陰陽離子液胞 47
3-2-5 液胞雙層膜之相轉移溫度 50
3-3 陰陽離子界面活性劑碳鏈長度與對稱性的影響 51
3-3-1 物理穩定性 51
3-3-2 界面電位 54
3-3-3 粒徑 54
3-3-4 雙層膜的排列 56
第四章 結論 95
參考文獻 97

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