臺灣博碩士論文加值系統

鎂合金因具有良好力學特性及生物可降解性，降解成分可刺激骨細胞活性，適合應用在人工植體上，有機會取代目前臨床使用之不銹鋼與鈦合金，被視為第三代生醫材料。但生醫鎂合金由於抗腐蝕性差，動物實驗顯示鎂合金本身降解速率過快，導致植體表面析氫速度太高與增加周圍溶液 pH值，引起身體異常反應。鎂合金另一個問題是表面改質不易，很難利用現有表面改質方式獲得良好抗蝕並具有生物親和性表面。
本研究使用高溫反應器進行水熱處理，使試片表面生成均質與緻密之氧化物保護膜，提高試片基材抗腐蝕能力。而新式T-BAG 製程可有效的接枝上單分子膜，藉由單分子膜技術改變官能基，利用表面官能基獲得不同的性質，因此本研究融合此二種表面處理技術，並各別探討經表面處理後的鎂合金抗蝕性及生物相容性的變化。首先利用水熱處理方式在生醫用鎂合金表面生成氫氧化鎂保護膜，提供基材具有抗腐蝕能力，接續利用 T-BAG 法進行改質，獲得疏水性的保護，在模擬體液下進行電化學試驗及浸泡試驗結果中，各項耐蝕性的指標皆有隨處理步驟有順序性明顯上升，證明水熱複合單分子塗層的表面可以改善鎂合金之抗腐蝕性。在生物親和性測試中，以細胞毒性、細胞形態、細胞增生能力及蛋白質吸附量進行評估，各組別皆無明顯細胞毒性，其他項目皆有明顯改善的效果。經過表面處理後細胞貼附能力及增生效果大大提高，最後由蛋白質吸附實驗證實材料表面因官能基及疏水性的影響，提高蛋白質吸附能力並提供骨細胞更良好的生長環境。由上述結果，本研究所研究之鎂合金表面處理技術可有效應用於可降解性骨科植體。

Magnesium (Mg) and its alloys are new biomaterials which have been studied recently for hard-tissue replacement. The advantages of magnesium alloy compared to other metal traditionally used in implant, such as stainless and titanium alloy. Magnesium alloy have good mechanical properties and biodegradable. In addition, magnesium is an essential component in human metabolism, and magnesium is known to play an important role in the bone formation. However, owing to high electrochemical corrosion, the poor corrosion resistance of magnesium alloy hinders their use in clinical application. Thus, surface treatment is indispensable to improve their corrosion resistance and biocompatibility. Compared to Ca/P deposition and anodic treatment, hydrothermal treatment could form a uniform, adhesive and protective oxide film on the surface of magnesium. The oxide film could protect magnesium alloys from the aggressive attack in human body. By T-BAG method, the hydrothermal oxide film can be modified with hydrophobic monolayers. The designed tail groups and molecules can be selected to form T-BAG for specific applications, such as corrosion resistance and biocompatibility.
In this investigation, attempt will be devoted to apply hydrothermal treatment to grow protective oxide films of magnesium alloy. After hydrothermal treatment, biocompatibility and protective hydrophobicity could be achieved by grafted functional groups on oxide film by T-BAG method.
The electrochemical and immersion test(in R-SBF) results indicated that anti-corrosion behavior can be enhanced by hydrophobic tail groups (-CH3) on hydrothermal coating surface of plate-like architecture. All the results suggest that the hydrothermal / T-BAG coating can effectively protect the magnesium alloy.
And the biocompatibility of magnesium alloy and coatings was studied by using human osteoblast-like MG-63 cells. It was found that the MG-63 cells could grow well on the surface of hydrothermal/T-BAG coated AZ61 and the evidently cell proliferation rate were provoked by protein adsorption in culture medium. It was concluded that the hydrothermal/T-BAG coated magnesium alloy had good biocompatibility.

摘要 I
Abstract II
誌謝 IV
第一章 緒論 1
1-1生醫材料之發展 2
1-2鎂合金生醫材料之優點 2
1-3鎂合金生醫材料之缺點 4
1-4鎂合金提升抗腐蝕性處理 5
1-5鎂合金表面改質技術 6
1-6生醫材料表面特性對於細胞的影響 7
1-7單分子膜之製備技術 9
1-7-1 Langmuir-Blodgett 法 9
1-7-2自組裝單分子膜 10
1-7-3 T-BAG法 11
2研究目的 13
第二章 實驗內容及方法 14
2-1實驗流程 14
2-1-1實驗藥品 15
2-1-2實驗儀器 17
2-2-1試片前處理 18
2-2-2試片鹼液鈍化前處理 18
2-2-3製備水熱塗層 19
2-2-4 T-BAG單分子膜製程 20
2-2-4-1 T-BAG夾具製作 20
2-2-4-2單分子膜溶液配製 20
2-2-4-3 T-BAG 單分子膜改質 20
2-3 材料表面特性分析 21
2-3-1 材料表面形態及元素分析 21
2-3-2 材料表面相組成分析 22
2-3-3親疏水性測試 22
2-3-4塗層貼附力強度測試 23
2-3-5表面化學鍵結分析 23
2-4 電化學腐蝕測試 24
2-4-1模擬體液(R-SBF) 24
2-4-2開路電位對時間變化之測試 24
2-4-3電化學交流阻抗頻譜測試(EIS) 25
2-4-4動電位極化曲線測試 25
2-5模擬體液浸泡腐蝕測試 26
2-5-1模擬體液浸泡失重量測 26
2-5-2表面腐蝕形貌及元素組成 27
2-5-3表面腐蝕相組成 27
2-6體外細胞測試(in vitro test) 27
2-6-1細胞培養 28
2-6-2細胞毒性分析 28
2-6-3細胞增生活性分析 29
2-6-4蛋白質吸附定量分析 30
第三章 結果與討論 32
3-1水熱塗層製程及形貌分析 32
3-2 T-BAG單分子膜接枝及形貌分析 33
3-3 元素分析 34
3-4 相組成分析 35
3-5 接觸角試驗 36
3-6 XPS分析 37
3-7 塗層貼附力測試 39
3-8模擬體液中的電化學腐蝕 40
3-8-1開路電位變化 40
3-8-2交流阻抗測試 41
3-8-3動電位極化曲線測試 42
3-9模擬體液浸泡測試 44
3-9-1 pH值變化 44
3-9-2 試片重量變化及腐蝕速率 45
3-9-3腐蝕表面形貌及元素分析 46
3-9-4腐蝕表面相組成分析 47
3-10體外細胞試驗 49
3-10-1細胞毒性試驗 49
3-10-2細胞形態 50
3-10-3細胞增生測試 52
3-10-4蛋白質吸附測試 53
第四章 結論 54
參考文獻 56

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