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研究生:陳志文
研究生(外文):Chee-WoonTan
論文名稱:抗菌型生醫陶瓷性質探討
論文名稱(外文):Investigation of Antibacterial Properties of Bioceramic
指導教授:陳瑾惠朱建平朱建平引用關係
指導教授(外文):Jiin-Huey Chern LinJiin-Huey Chern Lin
學位類別:碩士
校院名稱:國立成功大學
系所名稱:材料科學及工程學系碩博士班
學門:工程學門
學類:材料工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:127
中文關鍵詞:抗菌鹽類金黃色葡萄球菌生醫陶瓷
外文關鍵詞:Antimicrobial saltsStaphylococcus aureusBioceramic
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為了降低骨科手術術後感染的風險,因而本實驗進行抗菌型生醫陶瓷的探討,使用抗菌鹽類當添加劑加入具有良好生物相容性與骨誘導性的雙相磷酸鈣與硫酸鈣的複合材中,製作成塊狀生醫陶瓷。
本實驗使用金黃色葡萄球菌當抑菌圈測試實驗的菌種,測試抗菌型生醫陶瓷的抗菌效果。首先探討四種抗菌鹽類添加入複合材後的抗菌效果。然後選擇抗菌效果較好與溶解速率較慢的C型抗菌鹽類添加入複合材中,探討此生醫陶瓷的各種性質。為了延緩抗菌鹽類在Hanks’solution中的釋放,本實驗配製成含有高分子的磷酸氫二銨((NH4)2HPO4)硬化劑,簡稱為P1、P2及P3,與C型抗菌鹽類及複合材混合製作成塊狀生醫陶瓷。
本實驗發現隨著添加高分子的量增加,塊狀生醫陶瓷的抗菌效果維持天數也增長。由未添加高分子的只能維持1~2天,增加至3~7天。含P3高分子的C型生醫陶瓷除了於第7天有微小的抑菌圈出現外,在浸泡Hanks’solution 10天後的細胞毒性測試中,對NIH-3T3細胞亦無不良反應,細胞存活率達85%以上,說明了此抗菌型生醫陶瓷具有潛質應用於骨頭修損上。

關鍵字:抗菌鹽類;金黃色葡萄球菌;生醫陶瓷

In order to reduce the risk of postoperative infection in orthopedic surgery, thus this experiment investigation antibacterial properties of bioceramic, we select antimicrobial salts additive to has good biocompatibility and bone-induced of biphasic calcium phosphate and calcium sulfate composite, and made into a block bioceramic.
In this study, we select the Staphylococcus aureus strains as the zone of inhibition test experiments, to test the antibacterial effect of the bioceramics. First, we test the antibacterial effect of four antimicrobial salts into the composite. Then, we select have better antibacterial effect and slower dissolution rate of C-type antimicrobial salts add into the composite material to explore the various properties of the bioceramics. In order to delay the release of antimicrobial salts in Hanks’solution, this experiment is formulated into the polymer-containing diammonium phosphate ((NH4)2HPO4) hardener, referred to as P1, P2, and P3, and C-type antimicrobial salts and composite materials mixed into a block bioceramics.
This was found, as the block bioceramic add the amount of the polymer increased, the antibacterial effect of maintain number of days also increased. Not add any polymer for 1~2 days, and after add the amount of the polymer increased to 3~7 days. Bioceramics of P3 and C-type antimicrobial salts, had a small zone of inhibition in seven days, the cytotoxicity test in the tenth day, NIH-3T3 cells had no adverse reactions, cell viability was 85% or more, indicating this antibacterial bioceramics have the potential to be applied on bone repair damage.

Keywords: Antimicrobial salts;Staphylococcus aureus;Bioceramic

摘要 I
ABSTRACT II
誌謝 III
總目錄 IV
圖目錄 IX
表目錄 XII
第一章 總序論 1
1-1 生醫材料的定義 1
1-2 生醫材料的分類 2
1-2-1 依製造原料及化學組成分類 2
1-2-2 依活性分類 5
1-3 生醫材料之發展 9
1-4 生醫陶瓷 13
1-5 人體硬組織成分及性質簡介 16
1-6 骨科植入材的種類及性質要求 23
1-6-1 骨科植入材的來源種類 23
1-6-2 骨取代物在生物學上的要求 24
1-6-3 骨取代材的性質及製程要求 25
1-7 抗菌型骨科植入材 26
1-7-1 抗菌型陶瓷植入材 26
1-7-2 抗菌型金屬植入材 27
1-8 骨骼發炎 27
1-9 金黃色葡萄球菌 30
1-10 抗菌劑與抗菌機制 33
1-11 可分解性高分子 37
第二章 文獻回顧 41
2-1 生醫陶瓷之骨取代材 41
2-1-1 生物活性與結構型態間之相關性 42
2-1-2 可吸收性生醫陶瓷 43
2-2 硫酸鈣的歷史回顧與性質簡介 47
2-2-1 硫酸鈣的歷史回顧與發展 47
2-2-2 硫酸鈣的性質簡介 48
2-3 磷酸鈣的性質簡介與發展 48
2-3-1 鈣磷系生醫陶瓷的性質與分類 48
2-3-2 雙相磷酸鈣骨水泥 51
2-3-3 磷酸鈣骨水泥的優點與缺點 52
2-4 骨科植入醫材的感染風險 57
2-5 骨科植入醫材抗菌對策文獻回顧 58
2-6 研究目的 61
第三章 實驗原理與步驟 62
3-1 實驗所使用的材料與藥品簡介 62
3-1-1 塊狀抗菌型生醫陶瓷基底材料 62
3-1-2 添加劑 62
3-1-3 硬化劑P1, P2及P3與浸泡溶液 62
3-1-4 TSB液態細菌培養基 62
3-1-5 TSB固態細菌培養基 63
3-2 實驗步驟 65
3-2-1 各種抗菌型鹽類之塊狀試片製作 65
3-2-2 含有抗菌型鹽類之塊狀生醫陶瓷試片製作 65
3-2-3 含P1, P2, P3之C型塊狀抗菌生醫陶瓷試片的製作 65
3-3 實驗原理與分析方法 69
3-3-1 抑菌圈之抗菌測試 69
3-3-2 pH值的量測 70
3-3-3 重量損失量測 71
3-3-4 孔隙率的量測 71
3-3-5 徑向抗壓強度測試 72
3-3-6 X-Ray diffraction(XRD)分析 73
3-3-7 SEM破斷面顯微觀察 74
3-3-8 細胞毒性測試 75
第四章 結果與討論 83
4-1 各種不同抗菌型鹽類之抑菌圈測試 83
4-2 添加不同抗菌型鹽類之塊狀生醫陶瓷 84
4-2-1 抑菌圈測試 84
4-2-2 pH值之量測 85
4-3 添加O型或C型抗菌型鹽類之塊狀生醫陶瓷 86
4-3-1 添加O型抗菌型鹽類之塊狀抗菌型生醫陶瓷的抑菌圈測試 86
4-3-2 C型抗菌型鹽類與一般鹽類(a or b)之抑菌圈測試比較 87
4-3-3 試片不同作法以延緩C型抗菌鹽類的釋放 88
4-4 添加P1,P2,P3之C型塊狀抗菌型生醫陶瓷 89
4-4-1 抑菌圈測試結果 89
4-4-2 pH值之量測 91
4-4-3 重量損失率量測 91
4-4-4 孔隙率量測 92
4-4-5 徑向抗壓強度(DTS)測試 92
4-4-6 SEM破斷面顯微觀察 93
4-4-7 細胞毒性測試 93
第五章 結論 118
參考文獻 119


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