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研究生:林依鈴
研究生(外文):Yi-LingLin
論文名稱:臺灣環境水體中類雌激素/類雄激素物質之流布
論文名稱(外文):The distribution of estrogenic and androgenic compounds in the water environment of Taiwan
指導教授:周佩欣
指導教授(外文):Pei-Hsin Chou
學位類別:碩士
校院名稱:國立成功大學
系所名稱:環境工程學系碩博士班
學門:工程學門
學類:環境工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:123
中文關鍵詞:類雌激素類雄激素生物試驗法
外文關鍵詞:Estrogenic compoundsAndrogenic compoundsBioassay
相關次數:
  • 被引用被引用:3
  • 點閱點閱:369
  • 評分評分:
  • 下載下載:32
  • 收藏至我的研究室書目清單書目收藏:1
本研究利用基因重組酵母菌生物試驗法搭配液相層析串聯式質譜儀,檢測臺灣河川及工業污水處理廠出流水中類雌激素及類雄激素物質於不同月份之分布情形。採樣地點分別為淡水河、鳳山溪、頭前溪、客雅溪、北港溪、朴子溪、急水溪、將軍溪、鹽水溪、二仁溪、阿公店溪、高屏溪、東港溪,及臺灣南部一科技園區內之污水處理廠處理單元出流水及與鄰近承受河川水體樣本。
生物試驗法結果顯示,鹽水溪流域支流潭頂溪、阿公店溪流域 (前洲橋)及高屏溪流域支流武洛溪之濕季水相樣本具高類雌激素活性,其餘採樣河川以乾季水相樣本具較高之類雌激素活性;懸浮固體樣本僅於乾季之二仁溪及阿公店溪中測出較明顯之類雌激素活性。所有河川之乾濕季水相樣本則皆無明顯之類雄激素活性。工業污水處理廠廢水經各處理單元處理後,其排放水中類雌激素物質濃度明顯下降。
液相層析串聯式質譜儀分析結果顯示,13條採樣河川之乾濕季水相樣本及懸浮固體中皆存在較高濃度之合成類雌激素雙酚A (32 ng/L~725.3 μg/L及7 ng/L~9.7 μg/L)及壬基酚 (nd~4.9 μg/L及nd~795 ng/L),其中以二仁溪之污染最為嚴重。此外,河川中天然雌激素雌酮、雌二醇及雌三醇以高屏溪流域之含量較高 (nd~831 ng/L),而低濃度之乙炔雌二醇僅於少數地點測得 (nd~51 ng/L)。以儀器分析結果比對生物試驗法之類雌激素活性,可得知天然水體中尚存在有未知之類雌激素污染物質,須進一步利用儀器分析鑑定其餘類雌激素物質。

In this study, yeast-based reporter gene assays and LC-MS/MS were used to detect the distribution of estrogenic and androgenic compounds in the river water and industrial wastewater samples of Taiwan collected at different months. The sampling sites included 13 rivers, different treatment units of an industrial wastewater treatment plant (WTP), and the receiving river of treated effluent from the industrial park.
The results of bioassay analysis showed that the water samples of AGD River and the tributaries of YS River and GP River exhibited high estrogenic activities in wet seasons. Other river water samples and the suspended solids (SS) samples of ER River and AGD River showed significant estrogenic activities in dry seasons. However, no significant androgenic activities were detected in samples collected at dry or wet seasons. In addition, the estrogenicity of WTP effluent decreased significantly after wastewater treatment.
The results of LC-MS/MS analysis showed that high concentrations of BPA (32 ng/L~725.3 μg/L and 7 ng/L~9.7 μg/L) and NP (nd~4.9 μg/L and nd~795 ng/L) were detected in the river water and SS samples, especially in ER River. Moreover, higher concentrations of E1, E2 and E3 were detected in GP River (nd~831 ng/L), and EE2 (nd~51 ng/L) were only detected at few sites. Comparing the results of bioassay and instrumental analysis, it’s obvious that there still exist unknown estrogenic compounds in the rivers, thus further identification of unknown estrogenic compounds is necessary.

中文摘要 III
英文摘要 IV
誌謝 V
表目錄 VIII
圖目錄 IX
第一章 前言 1
1-1研究動機 1
1-2研究目的 2
第二章 文獻回顧 3
2-1 內分泌干擾物質 3
2-1-1 類雌激素物質 4
2-1-2 類雄激素物質 7
2-1-3 內分泌干擾物質之危害與影響 9
2-1-4 國內外天然水體中天然及合成雌/雄激素濃度比較 11
2-2 生物試驗法 13
2-2-1 活體內試驗法 (in-vivo) 13
2-2-2 活體外試驗法 (in-vitro) 14
2-3 儀器分析法 17
2-3-1 液相層析質譜儀及應用 17
2-4 檢測河川流域及污水處理廠簡介 19
2-4-1 淡水河 20
2-4-2 鳳山溪 21
2-4-3 頭前溪 21
2-4-4 客雅溪 22
2-4-5 北港溪 22
2-4-6 朴子溪 23
2-4-7 急水溪 23
2-4-8 將軍溪 24
2-4-9 鹽水溪 24
2-4-10 二仁溪 25
2-4-11 阿公店溪 26
2-4-12 高屏溪 26
2-4-13 東港溪 27
2-4-14 工業污水處理廠 27
第三章 實驗步驟與方法 28
3-1 樣本採集 28
3-1-1 河川樣本採集方式 28
3-1-2 河川採樣位置 30
3-1-3 污水處理廠水樣採集 34
3-2 實驗材料與設備 35
3-2-1 實驗材料 35
3-2-2 實驗設備 38
3-3 實驗步驟 39
3-3-1 前處理 39
3-3-2 生物試驗法 41
3-3-3 高效液相層析儀分析 49
3-3-4液相層析串聯式質譜儀分析 50
3-3-5 回收率 55
第四章 結果與討論 56
4-1類雌激素生物試驗法分析結果 56
4-1-1 天然河川水相樣本 56
4-1-2 天然河川懸浮固體樣本 61
4-1-3 污水處理廠水相樣本 66
4-1-4 污水處理廠懸浮固體樣本 68
4-2類雄激素生物試驗法分析結果 69
4-3液相層析串聯式質譜儀分析結果 73
4-3-1 天然河川水相樣本 73
4-3-2 污水處理廠水相樣本 88
4-3-3 天然河川懸浮固體樣本 90
4-3-4 河川複合樣本分析 94
4-4 高效液相層析儀分析結果 96
4-4-1天然河川水相樣本 96
4-4-2污水處理廠水相樣本 110
第五章 結論與建議 113
5-1 結論 113
5-2 建議 115
參考文獻 116

1.Kinani S, Bouchonnet S, Creusot N, Bourcier S, Balaguer P, Porcher J-M, Aït-Aïssa S: Bioanalytical characterisation of multiple endocrine- and dioxin-like activities in sediments from reference and impacted small rivers. Environmental Pollution 2010, 158(1):74-83.
2.Chang H-S, Choo K-H, Lee B, Choi S-J: The methods of identification, analysis, and removal of endocrine disrupting compounds (EDCs) in water. Journal of Hazardous Materials 2009, 172(1):1-12.
3.Schiffer B, Daxenberger A, Meyer K, Meyer HHD: The Fate of Trenbolone Acetate and Melengestrol Acetate after Application as Growth Promoters in Cattle: Environmental Studies. Environ Health Perspect 2001, 109(11).
4.Ternes TA, Kreckel P, Mueller J: Behaviour and occurrence of estrogens in municipal sewage treatment plants — II. Aerobic batch experiments with activated sludge. Science of The Total Environment 1999, 225(1–2):91-99.
5.Chen C-Y, Wen T-Y, Wang G-S, Cheng H-W, Lin Y-H, Lien G-W: Determining estrogenic steroids in Taipei waters and removal in drinking water treatment using high-flow solid-phase extraction and liquid chromatography/tandem mass spectrometry. Science of The Total Environment 2007, 378(3):352-365.
6.Shappell NW, Billey LO, Forbes D, Matheny TA, Poach ME, Reddy GB, Hunt PG: Estrogenic Activity and Steroid Hormones in Swine Wastewater through a Lagoon Constructed-Wetland System. Environmental Science & Technology 2006, 41(2):444-450.
7.Tan BLL, Hawker DW, Müller JF, Leusch FDL, Tremblay LA, Chapman HF: Comprehensive study of endocrine disrupting compounds using grab and passive sampling at selected wastewater treatment plants in South East Queensland, Australia. Environment International 2007, 33(5):654-669.
8.Williams RJ, Jürgens MD, Johnson AC: Initial predictions of the concentrations and distribution of 17β-oestradiol, oestrone and ethinyl oestradiol in 3 English rivers. Water Research 1999, 33(7):1663-1671.
9.Cederroth CR, Zimmermann C, Nef S: Soy, phytoestrogens and their impact on reproductive health. Molecular and Cellular Endocrinology 2012(0).
10.Matsumoto T, Kobayashi M, Moriwaki T, Kawai Si, Watabe S: Survey of estrogenic activity in fish feed by yeast estrogen-screen assay. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2004, 139(1–3):147-152.
11.Jenkins RL, Wilson EM, Angus RA, Howell WM, Kirk M, Moore R, Nance M, Brown A: Production of Androgens by Microbial Transformation of Progesterone in Vitro:A Model for Androgen Production in Rivers Receiving Paper Mill Effluent. Environ Health Perspect 2004, 112(4):1508-1511.
12.Calafat AM, Ye X, Wong L-Y, Reidy JA, Needham LL: Exposure of the U.S. Population to Bisphenol A and 4-tertiary-Octylphenol:2003–2004. Environmental Health Perspectives 2008, 116(7):39-44.
13.Urbatzka R, Cauwenberge Av, Maggioni S, Vigano L, Mandich A, Benfenati E, Lutz I, Kloas W: Androgenic and antiandrogenic activities in water and sediment samples from the river Lambro, Italy, detected by yeast androgen screen and chemical analyses. Chemosphere 2007, 67(8):1080-1087.
14.Sohoni P, Sumpter JP: Several environmental oestrogens are also anti-androgens. Journal of Endocrinology 1998, 158(13):327-339.
15.Birnbaum LS, Fenton SE: Cancer and Developmental Exposure to Endocrine Disruptors. Environmental Health Perspectives 2003, 111(4):389-394.
16.Rijk J, Bovee T, Groot M, Peijnenburg A, Nielen M: Evidence of the indirect hormonal activity of prohormones using liver S9 metabolic bioactivation and an androgen bioassay. Analytical and Bioanalytical Chemistry 2008, 392(3):417-425.
17.Perusquía M, Stallone JN: Do androgens play a beneficial role in the regulation of vascular tone? Nongenomic vascular effects of testosterone metabolites. Am J Physiol Heart Circ Physiol 298 2010(8):H1301–H1307.
18.Blasco M, Carriquiriborde P, Marino D, Ronco AE, Somoza GM: A quantitative HPLC–MS method for the simultaneous determination of testosterone, 11-ketotestosterone and 11-β hydroxyandrostenedione in fish serum. Journal of Chromatography B 2009, 877(14–15):1509-1515.
19.Horiguchi T, Shiraishi H, Shimizu M, Morita M: Imposex and organotin compounds in Thais clavigera and T. bronni in Japan. Journal of the Marine Biological Association of the United Kingdom 1994, 74(03):651-669.
20.International Convention on the Control of Harmful Anti-fouling Systems on Ships [http://www.imo.org]
21.Wilson VS, Lambright C, Ostby J, Jr. LEG: In Vitro and in Vivo Effects of 17β-Trenbolone: A Feedlot Effluent Contaminant. Toxicological Sciences 2002, 70:202-211.
22.W.Dallinga J, J.C.Moonen E, C.M.Dumoulin J, L.H.Evers J, P.M.Geraedts J, C.S.Kleinjans J: Decreasing human semen quality and organochlorine compounds in blood, Hum. Human Reproduction 2002, 17(8):1973-1979.
23.Hu W-Y, Shi G-B, Hu D-P, Nelles JL, Prins GS: Actions of estrogens and endocrine disrupting chemicals on human prostate stem/progenitor cells and prostate cancer risk. Molecular and Cellular Endocrinology 2012, 354(1–2):63-73.
24.Prins GS, Tang W-Y, Belmonte J, Ho S-M: Perinatal Exposure to Oestradiol and Bisphenol A Alters the Prostate Epigenome and Increases Susceptibility to Carcinogenesis. Basic & Clinical Pharmacology & Toxicology 2008, 102(2):134-138.
25.Nelson ER, Habibi HR: Functional Significance of Nuclear Estrogen Receptor Subtypes in the Liver of Goldfish. Endocrinology 2010, 151:1668-1676.
26.Hansen PD, Dizer H, Hock B, Marx A, Sherry J, McMaster M, Blaise C: Vitellogenin – a biomarker for endocrine disruptors. TrAC Trends in Analytical Chemistry 1998, 17(7):448-451.
27.Soares J, Coimbra AM, Reis-Henriques MA, Monteiro NM, Vieira MN, Oliveira JMA, Guedes-Dias P, Fontaínhas-Fernandes A, Parra SS, Carvalho AP et al: Disruption of zebrafish (Danio rerio) embryonic development after full life-cycle parental exposure to low levels of ethinylestradiol. Aquatic Toxicology 2009, 95(4):330-338.
28.Sárria MP, Soares J, Vieira MN, Filipe C. Castro L, Santos MM, Monteiro NM: Rapid-behaviour responses as a reliable indicator of estrogenic chemical toxicity in zebrafish juveniles. Chemosphere 2011, 85(10):1543-1547.
29.Sebire M, Katsiadaki I, Taylor NGH, Maack G, Tyler CR: Short-term exposure to a treated sewage effluent alters reproductive behaviour in the three-spined stickleback (Gasterosteus aculeatus). Aquatic Toxicology 2011, 105(1–2):78-88.
30.Hatef A, Alavi SMH, Abdulfatah A, Fontaine P, Rodina M, Linhart O: Adverse effects of bisphenol A on reproductive physiology in male goldfish at environmentally relevant concentrations. Ecotoxicology and Environmental Safety 2012, 76(0):56-62.
31.Sharma B, Patiño R: Effects of cadmium, estradiol-17β and their interaction on gonadal condition and metamorphosis of male and female African clawed frog, Xenopus laevis. Chemosphere 2010, 79(5):499-505.
32.Santos MM, Castro LFC, Vieira MN, Micael J, Morabito R, Massanisso P, Reis-Henriques MA: New insights into the mechanism of imposex induction in the dogwhelk Nucella lapillus. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2005, 141(1):101-109.
33.Sárria MP, Santos MM, Reis-Henriques MA, Vieira NM, Monteiro NM: The unpredictable effects of mixtures of androgenic and estrogenic chemicals on fish early life. Environment International 2011, 37(2):418-424.
34.Mortensen AS, Arukwe A: Modulation of xenobiotic biotransformation system and hormonal responses in Atlantic salmon (Salmo salar) after exposure to tributyltin (TBT). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2007, 145(3):431-441.
35.Jolly C, Katsiadaki I, Le Belle N, Mayer I, Dufour S: Development of a stickleback kidney cell culture assay for the screening of androgenic and anti-androgenic endocrine disrupters. Aquatic Toxicology 2006, 79(2):158-166.
36.王正雄, 張小萍, 黃壬瑰, 李宜樺, 王世冠, 洪文宗, 陳珮珊: 環境荷爾蒙-壬基苯酚殘留調查及其對雄鯉魚生理效應之研究. 環境檢驗所; 2001.
37.Cheng C-Y, Wu C-Y, Wang C-H, Ding W-H: Determination and distribution characteristics of degradation products of nonylphenol polyethoxylates in the rivers of Taiwan. Chemosphere 2006, 65(11):2275-2281.
38.彭柏頤, 周佩欣: 結合生物試驗法與LC-MS/MS檢測台灣河川中類雌激素物質之分布. 國立成功大學; 2011.
39.Chen T-S, Chen T-C, Yeh K-JC, Chao H-R, Liaw E-T, Hsieh C-Y, Chen K-C, Hsieh L-T, Yeh Y-L: High estrogen concentrations in receiving river discharge from a concentrated livestock feedlot. Science of The Total Environment 2010, 408(16):3223-3230.
40.Shue M-F, Chen F-A, Chen T-C: Total estrogenic activity and nonylphenol concentration in the Donggang River, Taiwan. Environmental Monitoring and Assessment 2010, 168(1):91-101.
41.Boyd GR, Palmeri JM, Zhang S, Grimm DA: Pharmaceuticals and personal care products (PPCPs) and endocrine disrupting chemicals (EDCs) in stormwater canals and Bayou St. John in New Orleans, Louisiana, USA. Science of The Total Environment 2004, 333(1–3):137-148.
42.Sodré FF, Pescara IC, Montagner CC, Jardim WF: Assessing selected estrogens and xenoestrogens in Brazilian surface waters by liquid chromatography–tandem mass spectrometry. Microchemical Journal 2010, 96(1):92-98.
43.Jardim WF, Montagner CC, Pescara IC, Umbuzeiro GA, Di Dea Bergamasco AM, Eldridge ML, Sodré FF: An integrated approach to evaluate emerging contaminants in drinking water. Separation and Purification Technology 2012, 84(0):3-8.
44.Pawlowski S, Ternes TA, Bonerz M, Rastall AC, Erdinger L, Braunbeck T: Estrogenicity of solid phase-extracted water samples from two municipal sewage treatment plant effluents and river Rhine water using the yeast estrogen screen. Toxicology in Vitro 2004, 18(1):129-138.
45.Beck I-C, Bruhn R, Gandrass J: Analysis of estrogenic activity in coastal surface waters of the Baltic Sea using the yeast estrogen screen. Chemosphere 2006, 63(11):1870-1878.
46.Liu S, Ying G-G, Zhao J-L, Chen F, Yang B, Zhou L-J, Lai H-j: Trace analysis of 28 steroids in surface water, wastewater and sludge samples by rapid resolution liquid chromatography–electrospray ionization tandem mass spectrometry. Journal of Chromatography A 2011, 1218(10):1367-1378.
47.Furuichi T, Kannan K, Giesy JP, Masunaga S: Contribution of known endocrine disrupting substances to the estrogenic activity in Tama River water samples from Japan using instrumental analysis and in vitro reporter gene assay. Water Research 2004, 38(20):4491-4501.
48.Funakoshi G, Kasuya S: Influence of an estuary dam on the dynamics of bisphenol A and alkylphenols. Chemosphere 2009, 75(4):491-497.
49.Basheer C, Lee HK, Tan KS: Endocrine disrupting alkylphenols and bisphenol-A in coastal waters and supermarket seafood from Singapore. Baseline / Marine Pollution Bulletin 2004, 48(7):1145–1167.
50.Ko E-J, Kim K-W, Kang S-Y, Kim S-D, Bang S-B, Hamm S-Y, Kim D-W: Monitoring of environmental phenolic endocrine disrupting compounds in treatment effluents and river waters, Korea. Talanta 2007, 73(4):674-683.
51.Engvall E, Perlmann P: ENZYME-LINKED IMMUNOSORBENT ASSAY, ELISA III. Quantitation of Specific Antibodies by Enzyme-Labeled Anti-Immunoglobulin in Antigen-Coated Tubes The Journal of Immunology 1971, 109(8):129-135.
52.美國環保署 (U.S. EPA) [http://www.epa.gov/]
53.Soto AM, Sonnenschein C, Chung KL, F. M, Fernandez, Olea N, Serrano FO: The E-SCREEN Assay as a Tool to Identify Estrogens: An Update on Estrogenic Environmental Pollutants. Environ Health Perspect 1995, 103:113-122.
54.Alum A, Yoon Y, Westerhoff P, Abbaszadegan M: Oxidation of bisphenol A, 17β-estradiol, and 17α-ethynyl estradiol and byproduct estrogenicity. Environmental Toxicology 2004, 19(3):257-264.
55.Svenson A, Allard A-S: In vitro androgenicity in pulp and paper mill effluents. Environmental Toxicology 2004, 19(5):510-517.
56.Legler J, Brink CEvd, Brouwer A, Murk AJ, Saag PTvd, Vethaak AD, Burg Bvd: Development of a stably transfected estrogen receptor-mediated luciferase reporter gene assay in the human T47D breast cancer cell line. Toxicological Sciences 1999, 48(12):55-66.
57.Houtman CJ, Sterk SS, van de Heijning MPM, Brouwer A, Stephany RW, van der Burg B, Sonneveld E: Detection of anabolic androgenic steroid abuse in doping control using mammalian reporter gene bioassays. Analytica Chimica Acta 2009, 637(1–2):247-258.
58.Campíns-Falcó P, Verdú-Andrés J, Sevillano-Cabeza A, Herráez-Hernández R, Molins-Legua C, Moliner-Martinez Y: In-tube solid-phase microextraction coupled by in valve mode to capillary LC-DAD: Improving detectability to multiresidue organic pollutants analysis in several whole waters. Journal of Chromatography A 2010, 1217(16):2695-2702.
59.Andrew MN, Dunstan RH, O’Connor WA, Van Zwieten L, Nixon B, MacFarlane GR: Effects of 4-nonylphenol and 17α-ethynylestradiol exposure in the Sydney rock oyster, Saccostrea glomerata: Vitellogenin induction and gonadal development. Aquatic Toxicology 2008, 88(1):39-47.
60.Mazellier P, Méité L, Laat JD: Photodegradation of the steroid hormones 17β-estradiol (E2) and 17α-ethinylestradiol (EE2) in dilute aqueous solution. Chemosphere 2008, 73(8):1216-1223.
61.Vega-Morales T, Sosa-Ferrera Z, Santana-Rodríguez JJ: Determination of alkylphenol polyethoxylates, bisphenol-A, 17α-ethynylestradiol and 17β-estradiol and its metabolites in sewage samples by SPE and LC/MS/MS. Journal of Hazardous Materials 2010, 183(1–3):701-711.
62.Twaddle NC, Churchwell MI, Vanlandingham M, Doerge DR: Quantification of deuterated bisphenol A in serum, tissues, and excreta from adult Sprague-Dawley rats using liquid chromatography with tandem mass spectrometry. Rapid Communications in Mass Spectrometry 2010, 24(20):3011-3020.
63.經濟部水利署全球資訊網-台灣重要河川 [http://www.wra.gov.tw/]
64.Lin AY-C, Tsai Y-T: Occurrence of pharmaceuticals in Taiwan's surface waters: Impact of waste streams from hospitals and pharmaceutical production facilities. Science of The Total Environment 2009, 407(12):3793-3802.
65.經濟部水利署: 水文年報歷年電子書; 2009.
66.新竹科學工業園區 [http://www.sipa.gov.tw]
67.張尊國, 王黎穎, 鄭百佑: 高科技產業之重金屬排放及其影響-新竹科學園區為例. 2004.
68.雲林縣政府全球資訊網 [http://www.yunlin.gov.tw]
69.行政院農業委員會: 行政院農業委員會. 2011.
70.台南市戶政生活服務網 [http://tnhr.tncg.gov.tw]
71.二仁溪再生願景整治管理系統 [http://ivy1.epa.gov.tw/runlet/entry/]
72.高雄市政府環境保護局 [http://www.ksepb.gov.tw/]
73.全國環境水質監測資訊網 [http://wq.epa.gov.tw]
74.行政院環境保護署環境檢驗所: 河川、湖泊及水庫水質採樣通則. In.; 2005.
75.行政院環境保護署環境檢驗所: 環境檢驗品管分析執行指引. In.; 2004.
76.行政院環境保護署環境檢驗所: 水中總溶解固體及懸浮固體檢測方法. In.; 2006.
77.Routledge EJ, Sumpter JP: Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen. Environmental Toxicology and Chemistry 1996, 15(3):241-248.
78.Oasis 固相萃取方法原理 [http://www.waters.com]
79.Rodbard D (ed.): Mathematics and statistics of ligand assay: An illustrated guide. New York: Masson; 1981.
80.Lien G-W, Chen C-Y, Wang G-S: Comparison of electrospray ionization, atmospheric pressure chemical ionization and atmospheric pressure photoionization for determining estrogenic chemicals in water by liquid chromatography tandem mass spectrometry with chemical derivatizations. Journal of Chromatography A 2009, 1216(6):956-966.
81.Chen F, Ying G-G, Yang J-F, Zhao J-L, Wang L: Rapid resolution liquid chromatography-tandem mass spectrometry method for the determination of endocrine disrupting chemicals (EDCs), pharmaceuticals and personal care products (PPCPs) in wastewater irrigated soils. Journal of Environmental Science and Health, Part B 2010, 45(7):682-693.
82.Hanselman TA, Graetz DA, Wilkie AC: Manure-Borne Estrogens as Potential Environmental Contaminants:  A Review. Environmental Science & Technology 2003, 37(24):5471-5478

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