跳到主要內容

臺灣博碩士論文加值系統

(35.172.136.29) 您好!臺灣時間:2021/08/02 18:14
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:黃銘嘉
研究生(外文):Meng-KaWong
論文名稱:利用自發螢光做口腔癌偵測之分析
論文名稱(外文):Analysis of oral cancer detection using endogenous auto-fluorescence
指導教授:詹寶珠詹寶珠引用關係
指導教授(外文):Pau-Choo Chung
學位類別:碩士
校院名稱:國立成功大學
系所名稱:電腦與通信工程研究所
學門:工程學門
學類:電資工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:英文
論文頁數:43
中文關鍵詞:自發螢光口腔癌
外文關鍵詞:Auto-fluorescenceoral cancerNADHFAD
相關次數:
  • 被引用被引用:0
  • 點閱點閱:210
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
近年來,口腔癌的發生率一直有上升的趨勢,這個類別的癌症大多發生在男性身上。加上它的死亡率一直以來都是男性國人裡均高居於前四名,並且口腔癌的發生率更是全球之冠,因此口腔癌是嚴重威脅男性的生命健康。但是在癌症初期階段被發現並給予治療的話,與其它癌症相比之下存活率也高出許多,因此早期診斷出口腔癌一直是醫學界希望達到的目標。然而目前主流的診斷方式都有其缺點,無法兼顧精確度與便利性,甚至需要侵入方式診斷。在本報告中,我們將發展一套利用內生自發螢光作為口腔癌偵測的方法,因此這套系統具有安全、非侵入性和快速檢測的特性我們希望藉由這套系統,提高男性們在早期發現口腔癌的機會。

在本研究中,我們使用激發內生自發螢光的技術進行不同的動物實驗,並且進一步探討不同生理狀態、正常組織和變異組織的內生自發螢光量的多寡,從早期使用光譜儀測量到最後使用成像系統測量各不同生理狀態的組織,並分析這些組織的自發螢光。我們除了使用不同濃度自發螢光的水溶液了解螢光的亮度與濃度之間的關係外,並且測量了不同生理狀態的老鼠間螢光的亮度差異,最後我們還分析正常組織和變異組織間螢光的亮度。

In recent years, the incidence of oral cancer has continued an upward trend, this category of cancer occurs mostly in men. And its mortality rate in men has always been a high living in the top four, the incidence of oral cancer is highest in the world; threaten seriously male life and health. But in the early stages of cancer was discovered and given the treatment, the survival rates compare with other cancer is much higher, so early diagnosis of oral cancer has been the medical community want to achieve. However, the current mainstream diagnostic methods has shortcoming, cannot take into account the accuracy and convenience, or even requires invasive way to diagnostic. In this report, we will develop a set of using the endogenous auto-fluorescence as methods of oral cancer detection, and a characteristic of this system is safe, non-invasive and rapid detection of the features. We hope that through this system to improve opportunities for people to early detection of oral cancer.
In this paper, we use technology to excite the endogenous auto-fluorescence in different animal experiments, to find out more about the amount of emitted auto-fluorescence on different physiological states, normal and abnormal tissues, form the early use of the spectrometer of the different physiological states of these tissue and analysis a amount of auto-fluorescence. In addition to we used different concentration of solution to know the relationship between the amount of light and concentration for auto-fluorescence, and measuring in disparity physiological states of mice to observe fluorescent brightness. Finally, we also analyses the fluorescence between normal tissues and abnormal tissue of mice.

Contents v
List of Figures vi
List of Tables vii
Chapter 1 Introduction 1
1.1 Motivation 1
1.2 Current development 3
1.3 Research goals and system architecture 4
Chapter 2 Related literatures 7
2.1 Biological tissue auto-fluorescence molecules in different states 9
2.2 The relationship between autofluorescence and lesion 10
2.3 Relative research 12
Chapter 3 Simulation Theory 14
3.1 The simulation of autofluorescence on tissue 16
Chapter 4 Experiment design and result 17
4.1.1 Water solution experiment 17
4.1.2 The architecture of the water solution experimental system 18
4.2.1 The result of water solution experiment 20
4.2.2 Measuring mice under different metabolic status experiment 22
4.2.3 The process of animal experiment 23
4.2.4 Animal experiments of image analysis 24
4.2.5 The result of animal experiment 26
4.3.1 Monte Carlo simulation experiment 29
4.4.1 The oral of animal experiment 31
4.4.2 Compared with other studies 36
Chapter 5 Conclusion and Future work 37
5.1 Conclusion 37
5.2 Future work 38
References 39



[1]Barrellier P, Babin E, Louis MY, Meunier-Guttin A. “The use of toluidine blue in the diagnosis of neoplastic lesions of the oral cavity. Rev Stomatol Chir Maxillofac, No.94 pp.51–4 1993.
[2]Mark W. Lingen; John R. Kalmar; Theodore Karrison; Paul M. Speight. “Critical Evaluation of Diagnostic Aids for the detection of Oral Cancer. Oral Oncol, no.44, pp.10-22, 2008.
[3]Onizawa K, Yoshida H, Saginoya H. “Chromatic analysis of autofluorescence emitted from squamous cell carcinomas arising in the oral cavity: a preliminary study. Int J Oral Maxillofac Surg, no.1,pp.42-46 2000.
[4]Lam S, MacAulay C, Hung J, LeRiche J, Profio AE, Palcic B. “Detection of dysplasia and carcinoma in situ with a lung imaging fluorescence endoscope device. J Thorac Cardiovasc Surq, no.6, pp.1035-1040 1993.
[5]Ralph S Dacsota, Brian C Wilson, Norman E Marcon, “New optical technologies for earlier endoscopic diagnosis of premalignant gastrointestinal lesions Journal of Gastroenterology and Hepatology, no.17, pp85-104 2002.
[6]Juan Manuel Benavide, Sung Chang, Sun Young Park, and Rebecca Richards-Kortum “Multispectral digital colposcopy for in vivo detection of cervical cancer. Optics Express, no.10, pp.1223-1236 2003.
[7]Chance B. “Spectrophotometry of intracellular respiratory pigments. Science, no.3124, pp.767-775 1954.
[8]Sundt TM, Anderson RE. “Reduced nicotinamide adenine dinucleotide fluorescence and cortical blood flow in ischemic and nonischemic squirrel monky cortex.I. Animal preparation, instrumentation, and validity of model. Stroke, no.3, pp.270-278 1975.
[9]Harbig K, Chance B, Kovach AGB, Reivich M. “In vivo measurement of pyridine nucleotide fluorescence from cat brain cortex. Journal of Applied Physiology, no.4, pp.480-488 1976.
[10]Chance B. “Oxidation-Reduction Ratio Studies of Mitochondria in Freeze-trapped Samples. The Journal of biological chemistry, no.11, pp.4764-4771 1979.
[11]He N. Xu Shoko Nioka Jerry D. Glickson Chance B. Lin Z. Li, “Quantitative mitochondrial redox imaging of breast cancer metastatic potential. Journal of biological optics, no.3, pp.036-046 2010.
[12]He N. Xu Shoko Nioka Jerry D. Glickson Chance B. Lin Z. Li, “Calibration of CCD-based redox imaging for biological tissues. Proceedings of SPIE , no.1 ,Vol. 7262 2009.
[13]Juan Manuel Benavides, Sung Chang, Sun Young Park, and Rebecca Richards-Kortum, “Multispectral digital colposcopy for in vivo detection of cervical cancer. Optics express, No.10, pp.1223-1236 2003.
[14]Chance, B., Shoko Nioka, A. Quo, and Jenq-Ruey Horng, “A novel time-shared fluorometer gives the mitochondrial redox stage as the ratio of two components of the respiratory chain of the animal and human buccal cavity with quantitative measures of the redox energy state., Journal of Innovative Optical Health Sciences, No. 4 pp.235–245 2010.
[15]Chance, B. “Quantitative aspects of the control of oxygen utilization CibaFoundation Symposium on Cell Metabolism. J. & A.Churchill Ltd., London, No.10, pp.91-129 1959.
[16]C. Skala, Kristin M. Riching, Annette Gendron-Fitzpatrick, Jens Eickhoff, Kevin W. Eliceiri, John G. White, and Nirmala Ramanujam. “In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia Melissa The National Academy of Sciences of the USA, no.104, pp.19494–19499 2007
[17]Chance, B., Williams, G.R. “A method for the localization of sites for oxidative phosphorylation. Nature, no.176, pp.250–254 1955.
[18]Chance, B., Williams, G.R., “Respiratory enzymes in oxidative phosphorylation (I – Kinetics of oxygen utilization). J. Biol. Chem, No.217, pp.383–393 1955.
[19]Chance, B., Williams, G.R. “Respiratory enzymes in oxidative phosphorylation (II- Difference spectra). J. Biol. Chem, No.217, pp.395–407 1955.
[20]Chance, B., Williams, G.R. “Respiratory enzymes in oxidative phosphorylation (III – The steady state). J. Biol. Chem, No.217, pp.409–427 1955.
[21]Chance, B., Williams, G.R. “Respiratory enzymes in oxidative phosphorylation (IV – The respiratory chain). J. Biol. Chem. No,217, pp.429–438 1955.
[22]Chance, B., Williams, G.R., Holmes, W.F., Higgins, J. “Respiratory enzymes in oxidative phosphorylation (V – A mechanism for oxidative phosphorylation). J. Biol. Chem, No.217, pp.439–451. 1955.
[23]Roblyer D, Kurachi C, Stepanek V, Williams MD, El-Naggar AK, Lee JJ, Gillenwater AM, Richards- Kortum R. “Objective detection and delineation of oral neoplasia using autofluorescence imaging. Cancer Prev Res (Phila Pa), No. 5, pp423-431 2009.
[24]R. Sepehra, S. Audib,c, K. Staniszewskia, S Malekia, M. Ranji.Fluorescence Spectroscopy and Cryoimaging of Rat Lung Tissue Mitochondrial Redox State. European Conference on Biomedical Optics, Volume 8087, pp. 80870A-6 2011.
[25]Ekaterina Svistun, Reza Alizadeh-Naderi, Adel El-Naggar, Rhonda Jacob, Ann Gillenwater, Rebecca Richards-Kortum, “Vision Enhancement system for detection of oral cavity neoplasia based on autofluorescence,Head and Neck, No.26, pp.205-215 2004.
[26]D.Wang, James M. Crawford, Michael S. Feld, Yang Wang, Irving Itzkan, Jacques Van Dam. In vivo identification of colonic dysplasia using fluorescence endoscopic imaging Thomas. Gastrointestinal Endoscopy, No.49, pp.447-455 1999.
[27]Seong G. Kong, Matthew E. Martin, and Tuan Vo-Dinh. “Hyperspectral Fluorescence Imaging for Mouse Skin Tumor Detection. ETRI Journal, Volume 28, pp.770-776 2006.
[28]Jyh-Kwei Chen; Che-Shoa Chang; Huihua Kenny Chiang. “Implementation of autofluorescence for normal oral mucosas, oral precencer lesions and squamous cell carcinomas differentiation. Nation Yang-Ming University Institute of Clinical Dentistry Master Thesis, Chapter 2, 2003.
[29]Mohammed Rahman,a,b Pankaj Chaturvedi,b Ann M. Gillenwater,c and Rebecca Richards-Kortuma. “Low-cost, multimodal, portable screening system for early detection of oral cancer. Journal of biological optics, No.13, pp.030502 2008.
[30]Mohammed S Rahman, Nilesh Ingole, Darren Roblyer, Vanda Stepanek, Rebecca Richards-Kortum, Ann Gillenwater, Surendra Shastri and Pankaj Chaturvedi. “Evaluationof low-cost, portable imaging system for early detection of oral cencer. Rahman et al. Head & Neck Oncology, No.2, pp2-10 2010.
[31]S. A. Prahl, M. Keijzer, S. L. Jacques, A. J. Welch. “A Monte Carlo Model of Light Propagation in Tissue. Proc. SPIE IS (6th edn.), No.5, pp.102–111 1989.
[32]Shi-Hao Hua; Huihua Kenney Chiang. “Double Integrating Sphere and Monte Carlo Simulation of Cervical Tissue Autofluorescence Spectra. National Yang-Ming University Institute of Diophotonics Engineering Master Thesis 2004.
[33]D. C. Walker, B. H. Brown, A. D. Blackett, J. Tidy, and R. H. Smallwood, ‘‘A study of the morphological parameters of cervical squamous epithelium,’’ Physiol. Meas. No.24, pp.121–135 2003.
[34]Sung K. Chang Dizem Arifler ;Rebekah Drezek; Michele Follen; Rebecca Richards-Kortum. “Analytical model to describe fluorescence spectra of normal and preneoplastic epithelial tissue: comparison with Monte Carlo simulations and clinical measurements Journal of biomedical optics. No.9, pp.511-522 2004.

連結至畢業學校之論文網頁點我開啟連結
註: 此連結為研究生畢業學校所提供,不一定有電子全文可供下載,若連結有誤,請點選上方之〝勘誤回報〞功能,我們會盡快修正,謝謝!
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top