臺灣博碩士論文加值系統

TLRs具有調節免疫，參與能量代謝、控制細胞呼吸以及提高骨骼肌粒線體生合成等作用。然而過去未曾有任何文獻指出TLR2與運動耐力表現之間的關係，故本研究透過野生型及TLR2全基因剔除小鼠模式來探討TLR2在運動表現方面所扮演的角色。本實驗採用C57BL/6J小鼠與TLR2基因剔除品系之小鼠，分為四組：(1)對照老化組(WT-S)；(2)對照成年組(WT-A)；(3) TLR-2剔除老化組(KO-S)；(4) TLR-2剔除成年組(KO-A)。針對前肢抓力測試、力竭運動表現、口服葡萄糖耐受試驗(OGTT)藉以瞭解葡萄糖利用情形進行分析。前肢抓力之運動表現結果，KO-S顯著低於WT-S，KO-A顯著低於WT-A，而TLR2基因剔除則顯著低於WT。單次負重游泳力竭之耐力表現測試結果，KO-A顯著低於WT-A，而TLR2基因剔除則顯著低於WT。口服葡萄糖耐受試驗之曲線下面積結果為KO-S顯著低於WT-S、TLR2基因剔除顯著低於WT。我們發現當小鼠缺乏TLR2 基因時，雖有較佳的胰島素敏感度，但會導致副睪脂肪囤積增加並降低對脂質的利用，進而引發全身系統性代謝與生理上的影響，最後導致運動表現不佳的情形發生。

TLRs have been demonstrated to regulate the immune system, energy metabolism, cellular respiration and increase to mitochondrial biogenesis in skeletal muscle. However, there is no literature reported on the relationship between TLR2 and exercise performance. The purpose of this study was to examine the effect of TLR2 on exercise performance by using the TLR2 knockout mouse model. In this study, wild-type (WT) and TLR2 konckout (KO) mice of the C57BL/6J genetic background were be used and divided into four groups: (1) wild-type and senescence (WT-S); (2) wild-type and adult (WT-A); (3) TLR-2 knockout and senescence (KO-S); (4) TLR-2 knockout and adult (KO-A). The forelimb grip strength, an exhaustive swimming exercise, and oral glucose tolerance test (OGTT) of all animals were be analyzed. The results showed that forelimb grip strength in group KO-S was significantly lower than group WT-S, KO-A group was significantly lower than WT-A group, and TLR2 knockout mice was significantly lower than the WT mice. The exhaustive swimming time of group KO-A significantly lower than that of group WT-A, and TLR2 knockout is significantly lower than the WT. The area under the curve of OGTT result of group KO-S was significantly lowered than group WT-S, and TLR2 knockout mice significantly lower than the WT mice. In the present study, we found that TLR2 knockout mice have better insulin sensitivity, but the visceral fat accumulation is also higher than the control mice. In conclusion, TLR2 play an important role in the balance of glucose and fat metabolism, and contribute to exercise performance.

目 錄

中文摘要…………………………………………………………………………Ⅰ
英文摘要…………………………………………………………………………Ⅱ
圖目錄……………………………………………………………………………Ⅷ
表目錄……………………………………………………………………………Ⅸ

第壹章 緒論.............................................1
第一節 研究動機 ....................................... 1
第二節 研究目的 ....................................... 3

第貳章 文獻探討.........................................4
第一節 能量利用 ....................................... 4
第二節 老化與氧化壓力 .................................. 6
第三節 TLR2 與胰島素阻抗之相關研究 ...................... 8

第參章 實驗方法.........................................9
第一節 實驗動物 ....................................... 9
第二節 實驗設計 ...................................... 10
第三節 分析步驟與方法 ................................. 12
第四節 統計分析 ...................................... 19

第肆章 實驗結果........................................20
第一節 生化代謝之變化 ................................. 25
一、口服葡萄糖耐受試驗(Oral Glucose Tolerance Test, OGTT) ............................................... 25
二、胰島素阻抗指數(Homeostasis Model Assessment, HOMA) ............................................... 27
第二節 運動表現之比較 ................................. 31
一、TLR-2基因與老化於前肢抓力表現之影響 ..............31
二、TLR-2基因與老化對於耐力表現之影響 ............... 32
第三節 血液生化及肝臟與肌肉之組織中肝醣含量情形 .......... 33
一、血液生化之比較 ................................ 33
二、肝臟肝醣之比較 ................................ 35
三、肌肉肝醣之比較 ................................ 36
第四節 組織切片之觀察 ................................ 37
一、肝臟切片之比較 ................................ 37
二、肌肉切片之比較 ................................ 38

第伍章 討論...........................................39
第一節 生化代謝之比較 ................................. 39
以口服葡萄糖耐受試驗(Oral Glucose Tolerance Test, OGTT)評估生化代謝之變化 ................................39
第二節 運動表現之比較 ..................................40
第三節 血液生化以及肝臟與肌肉之組織中分子變化情形 ......... 41
一、血液生化之比較 ................................. 41
二、肝臟與肌肉組織中肝醣之比較 ...................... 43
第四節 組織切片之比較 ................................. 44

第陸章 結論與建議......................................45
一、結論 ......................................... 45
二、建議 ......................................... 45

參考文獻...............................................46

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