跳到主要內容

臺灣博碩士論文加值系統

(18.97.14.81) 您好!臺灣時間:2025/02/11 01:04
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:朱為麟
研究生(外文):Chu, Wei-Lin
論文名稱:利用液晶聚合物薄膜之人類三酸甘油酯及高密度膽固醇感測器
論文名稱(外文):A human triglyceride sensor and a human high-density lipoprotein sensor by using a liquid crystal and polymer composite film
指導教授:林怡欣林怡欣引用關係
指導教授(外文):Lin, Yi-Hsin
學位類別:碩士
校院名稱:國立交通大學
系所名稱:光電工程學系
學門:工程學門
學類:電資工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:152
中文關鍵詞:三酸甘油酯高密度膽固醇生物感測器液晶聚合物薄膜高密度脂蛋白
外文關鍵詞:High density lipoprotein cholesterolTriglycerideHDL-CLCPCFliquid crystalbiosensor
相關次數:
  • 被引用被引用:0
  • 點閱點閱:509
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
本論文提出一個利用液晶聚合物薄膜之感測人類三酸甘油脂以及高密度膽固醇濃度之感測器。其感測原理是人類血清中三酸甘油脂濃度越高時,人類血清的表面張力降低,因此人類血清置於不加電壓的液晶聚合物薄膜表面時接觸角會隨三酸甘油脂濃度增加而降低。當人類血清含有高濃度的高密度膽固醇時,使高極性之高密度脂蛋白分子增多,因此當液晶聚合物薄膜受到電場驅動時,由於液晶分子具有極性的氰基與高密度脂蛋白分子具較強的附著力,使液晶聚合物薄膜表面聚合物顆粒間的液晶分子的氰基轉向表面,此液晶分子軸向轉動角度與高密度膽固醇的濃度成正比,因而影響到含有高濃度的高密度膽固醇之人類血清液珠於液晶聚合物薄膜上的運動。因此,我們可實現利用液晶聚合物薄膜之人類三酸甘油脂及高密度膽固醇濃度感測器。
In this thesis we demonstrate a human triglyceride sensor and a human high-density lipoprotein sensor by using a liquid crystal and polymer composite film (LCPCF). The sensing mechanism for the human triglyceride (TG) in the human serum is that the contact angle of a droplet of the human serum on LCPCF at voltage-off state decreases with an increase of TG concentration because the surface tension of the human serum decreases with an increase of TG concentration. The sensing mechanism for the high density lipoprotein- cholesterol (HDL-C) in the human serum is that the adhesion between high polar HDL-C and cyano-group of LC molecules which tilts up at voltage-on state increases when the concentration of HDL-C increases. The tilt angle or the orientation of LC molecules is affected linearly by the concentration of HDL-C. As a result, the droplets of the human serums with different concentrations of HDL-C on LCPCF have different droplet motion. Therefore, we can realize a human triglyceride sensor and a human high-density lipoprotein sensor by using LCPCF.
摘要 1
Abstract 2
目錄 4
圖目錄 9
表目錄 16
第一章 導論 17
1.1 血液以及心血管疾病 17
1.1.1 心血管疾病與發生原因 17
1.1.2 高血脂症的分類 20
1.1.3 HDL-C及TG的介紹 21
1.2 不同的血脂檢測方法 22
1.2.1 TG檢測方法 23
1.2.2 HDL-C檢測方法 36
1.3 液晶聚合物薄膜介紹 54
1.3.1 液晶介紹 54
1.3.3 液晶的電光特性 56
1.3.2 液晶聚合物薄膜(Liquid Crystal and Polymer Composite Film, LCPCF) 58
1.4 研究動機 61
第一章參考文獻 63
第二章 液體於表面的表現 71
2.1 表面張力 71
2.1.1 定義表面張力 71
2.1.2 Young-Laplace 定理 72
2.1.3 Young’s Equation 74
2.2 內聚力以及附著力(Work of Cohesion and Adhesion) 75
2.2.1 內聚力(Cohesive force) 75
2.2.2 附著力(Adhesive force) 76
2.3 表面水濕性(Surface Wettability) 78
2.4 接觸角遲滯現象(Contact angle hysteresis) 80
2.4.1 定義接觸角遲滯現象 81
2.4.2 接觸角遲滯現象成因 82
2.5 液體於表面之運動 83
2.6 利用LCPCF感測血清中TG及HDL-C之設計原理 84
第二章參考文獻 86
第三章 實驗樣品配置與實驗方法 88
3.1 液晶聚合物薄膜製作 88
3.2 實驗量測裝置 89
3.2.1 偏光顯微鏡 90
3.2.2 倒立式生物顯微鏡 92
3.2.3 掃描式電子顯微鏡 93
3.2.4 接觸角量測儀-First ten angstrom 1000 (FTA 1000) 95
3.2.5表面張力量測 95
3.3 血清樣品的量測流程圖 97
3.3.1 血清樣品的配置 98
3.3.2 血清樣品分析 99
3.3.3 血清於LCPCF上測試 100
3.4 血清樣品於液晶盒中與液晶接觸面之觀察 102
第三章參考文獻 102
第四章 實驗結果 104
4.1 液晶聚合物薄膜表面型態觀察 104
4.2 人類血清於液晶聚合物薄膜(LCPCF)表面之表現 106
4.2.1 調控過之人類血清於LCPCF之表現 106
4.2.2 一般人體血清於LCPCF表現 114
4.3 人類血清的表面張力 116
4.4 不同高密度膽固醇濃度血清與液晶界處觀察 117
第五章 實驗討論 123
5.1 不同濃度的TG對接觸角影響 123
5.1.1 不同濃度TG對於初始接觸角關係 123
5.1.2 不同濃度的TG對於LCPCF施加電壓後之接觸角關係 124
5.2 不同濃度之高密度膽固醇對於接觸角的影響 125
5.2.1 不同濃度之HDL-C與起始接觸角關係 125
5.2.2 不同濃度的HDL-C對於LCPCF施加電壓後之接觸角關係 127
5.3 高密度膽固醇與液晶接觸面之討論 128
5.4 血清於LCPCF上之表現 133
5.4.1 血清於未加電壓之LCPCF之前 133
5.4.2 血清於受電場影響的LCPCF 134
5.5 普遍人體血清於LCPCF上之表現 137
5.5.1 血清於LCPCF未加電壓表現 138
5.5.2血清於施加電壓之LCPCF表現 139
第五章參考文獻 139
第六章 LCPCF用以檢測TG以及HDL-C濃度高低 140
6.1 利用LCPCF製作人類TG以及HDL-C檢測器 140
6.1.1 未調控人類血清溶液於裝置未加電壓前接觸角檢測 140
6.1.2 未調控人類血清於裝置施加電壓後檢測 142
6.2 LCPCF TG及HDL-C感測器的優劣 143
6.3 LCPCF感測器及其他感測器比較 144
第七章 結論與展望 146
附錄A 149
附錄B 151
[1-1] World Health Organization, The Top Ten Causes of Death (2008) http://www.who.int/mediacentre/factsheets/fs310_2008.pdf
[1-2] V. Manninen, L. Tenkanen, P n. Koskinen, J. K. Huttunen, M. M?躪tt?鑼i, O. P. Heinonen, M. H. Frick, “Joint effects of serum triglyceride and LDL cholesterol and HDL cholesterol concentrations on coronary heart disease risk in the Helsinki Heart Study. Implications for treatment”, Circulation, 85:37-45 (1992)
[1-3] H. Robert Horton, et al., Principles of Biochemistry 3rd edition, (2002)
[1-4] National institutes of health, Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III) Final Report, Circulation,106:3143(2002)http://circ.ahajournals.org/content/106/25/3143.full.pdf
[1-5] B. G. Nordestgaard, M. Benn, P. Schnohr, A. Tybj?礽g-Hansen, “Nonfasting Triglycerides and Risk of Myocardial Infarction, Ischemic Heart Disease, and Death in Men and Women”, J. Am. Med. Assoc.,298(3):299-308. (2007)
[1-6] D. L. Sparks, and M. C. Phillips, “Quantitative measurement of lipoprotein surface charge by agarose gel electrophoresis”, J. Lipid Res. 33, 123-130. (1992)
[1-7] I. D. Cruzadoa, S. Songa, S. F. Crouseb, B. C. O'briena,and R. D. Macfarlanea, "Characterization and Quantitation of the Apoproteins of High-Density Lipoprotein by Capillary Electrophoresis", Anal. Biochem. 243, 100-109. (1996)
[1-8] A. S. Dukhin, et al., Ultrasound for characterizing colloids (2002).
[1-9] M. Burstein, H.R. Scholnick, "Lipoprotein-polyanion-metal interactions", Adv. Lipid. Res. , 11(0):67-108. (1973)
[1-10] H. Sugiuchi, Y. Uji, H. Okabe, T. Irie, K. Uekama, N. Kayahara and K. Miyauchi, "Direct measurement of high-density lipoprotein cholesterol in serum with polyethylene glycol-modified enzymes and sulfated alpha-cyclodextrin”, Clin. Chem. 41, 717-723. (1995)
[1-11] S. G. Klotzsch and J. R. McNamara, "Triglyceride Measurements: a Review of Methods and Interferences", Clin. Chem. 36, 9, 1605-13. (1990)
[1-12] E. V. Handel, D. B. Zilversmit, "Micromethod for the direct determination of serum triglycerides", J. Lab. Clin. Med. 50, 1, 152-7. (1957)
[1-13] Y. Liu, L. Mu, B. Liu, and J. Kong, "Controlled switchable surface", Chem. Euro. J. 11, 2622-2631. (2005).
[1-14] M. Eggstein, F. H. Kreutz, "Eine neue Bestimmung der Neutral fette in Blutserum und Gewebe. I. Mitteilung. Prinzip, Durchfuh rung und Besprechung der Methode", Klin. Wochenschr 44, 262-7. (1966)
[1-15]和光純藥工業株式會社. "Triglyceride 檢測用 使用說明書"(2004)
[1-16] S. I. Matsuura, R. Ishii, T. Itoh, S. Hamakawa, T. Tsunoda, T. Hanaoka,and F. Mizukami, “Immobilization of enzyme-encapsulated nanoporous material in a microreactor and reaction analysis”, Chem. Eng. J. 167, 744-749. (2011)
[1-17] I. Basu, R.V. Subramanian, A. Mathewa, A.M. Kayasthac, A. Chadha,and E. Bhattacharya, "Solid state potentiometric sensor for the estimation of tributyrin and urea", Sens. Actuators B 107 418–423. (2005)
[1-18]
[1-19] C. S. Lee, S. K. Kim,and M. Kim, “Ion-Sensitive Field-Effect Transistor for Biological Sensing”, Sensors, 9, 7111-7131. (2009)
[1-20] H. Vemulachedu, R. E. Fernandez, E. Bhattacharya,and A. Chadha, “Miniaturization of EISCAP sensor for triglyceride detection”, J. Mater. Sci-Mater. M. 20,S229–S234. (2009)
[1-21] A. Vijayalakshmia, Y. Tarunashreea, B. Baruwatib, S. V. Manoramab, B. L. Narayanaa, R.E.C. Johnsona,and N.M. Raoa, "Enzyme field effect transistor (ENFET) for estimation of triglycerides using magnetic nanoparticles”, Biosensors Bioelectron. 23, 1708-1714. (2008)
[1-22] P. R. Solanki, C. Dhand, A. Kaushik, A. A. Ansari, K. N. Sood, B. D. Malhotra, “Nanostructured cerium oxide film for triglyceride sensor”, Sens. Actuators B, 141, 551-556. (2009)
[1-23] W. Y. Liao, C. C. Liu, T. C. Chou, “Detection of triglyceride using an iridium nano-particle catalyst based amperometric biosensor ”, The Analyst, 133, 1757-63. (2008)
[1-24] G. R. Warnick, M. Nauck and N. Rifai, “Evolution of Methods for Measurement of HDL-Cholesterol: From Ultracentrifugation to Homogeneous Assays”, Clin. Chem. 47:9, 1579-1596(2001)
[1-25] L. Seigler and W. T. Wu,“Separation of Serum High-Density Lipoprotein for Cholesterol Determination:Ultracentrifugation vs Precipitation with Sodium Phosphotungstate and Magnesium Chloride”, Clin. Chem. 27:6 838-841.(1981)
[1-26] R. I. Levy and D. S. Fredrickson,“Heterogeneity of plasma high denstiy lipoprotein”,J. Clin. Invest.,44(3): 426–441. (1965)
[1-27] F. F. Reuss, "Sur un Nouvel Effet de l'?翼ectricit? Galvanique", Mem. Soc. Imp. Natur. Moscou, 2 ,327-337. (1809)
[1-28] M. von Smoluchowski, "Contribution to the theory of electro-.osmosis and related phenomena", Bull. Int. Acad. Sci. Cracovie 1,184-199. (1903)
[1-29] T. J. Bronzert, H. B. Brewer Jr. "New micromethod for measuring cholesterol in plasma lipoprotein fractions", Clin. Chem. 23:11, 2089-2098. (1977)
[1-30] G. L. Myers, G. R. Cooper, L. O. Henderson, D. J. Hassemer, M. M. Kimberly, "Standardization of lipid and lipoprotein measurement", Clin. Chem. 34(8B), B95-105.(1988)
[1-31] M. Nauck, K. Winkler, W. M?鑼z ,and H. Wieland, " Quantitative determination of high-, low-, and very-low-density lipoproteins and lipoprotein (a) by agarose gel electrophoresis and enzymatic cholesterol staining", Clin. Chem. 41, 1761–67. (1995)
[1-32] P. Benlian, C. Cansier, G. Hennache, O. Khallouf, P. Bayer, F. Duron, F. Carrat, R. Couderc, O. Chazouill?嫫es, J. Bardet, P. Bouchard, R. Poupon, J. Masliah ,and G. B?臆?膾iat, "Comparison of a new method for the direct and simultaneous assessment of LDL- and HDL-cholesterol with ultracentrifugation and established methods", Clin. Chem. 46, 493-505. (2000)
[1-33] M. Burstein, H. R. Scholnick, and R. Morfin, " Rapid method for the isolation of lipoproteins for human serum by precipitation with polyanions", J. Lipid Res. 11, 583–95. (1970)
[1-34] G. L. Myers, G. R.Cooper,L. O. Henderson, D. J.Hassemer, M. M. Kimberly, "Standardization of lipid and lipoprotein measurement. In:Rifai N, Warnick GR, Dominiczak MH, eds. Handbook of lipoprotein testing", Washington: AACC Press. 717–48. (2000)
[1-35] N. Harris, V. Galpchian ,and N. Rifai, "Three routine methods for measuring high-density lipoprotein cholesterol compared with the Reference Method", Clin. Chem. 42, 738–743. (1996)
[1-36] T. Kakuyama, S. Kimura, Y. Hasiguchi, "Fully automated determination of HDL-cholesterol from human serum with Hitachi 911", Clin. Chem. 40:A1104. (1994)
[1-37] H. Sugiuchi, Y. Uji, H. Okabe, T. Irie, K. Uekama, N. Kayahara and K. Miyauchi, "Direct measurement of high-density lipoprotein cholesterol in serum with polyethylene glycol-modified enzymes and sulfated a-cyclodextrin", Clin. Chem. 41, 717–23.(1995)
[1-38] 和光純藥工業株式會社. "Determiner-L HDL-C 檢測用 使用說明書"(2004)
[1-39] S. Izawa, M. Okada, H. Matsui, Y. Horita, "A new direct method for measuring HDL-cholesterol which does not produce any biased values", J. Med. Pharm. Sci. 37, 1385–88. (1997)
[1-40] N. Harris, V. Galpachian, J. Thomas, E. Iannotti, T. Law, N. Rifai, "Three generations of high-density lipoprotein cholesterol assays compared with ultracentrifugation/dextran sulfate-Mg 2+method", Clin. Chem. 43, 816–823. (1997)
[1-41] M. de Keijzer, D. Elbers, H. Baadenhuijsen, P. N. Demacker, "Evaluation of five different high-density lipoprotein cholesterol assays: the most precise are not the most accurate. Ann", Clin. Biochem. 36, 168–175. (1999)
[1-42] S. chunta, C. Promptmas, and C. Cherdchu, "Lipoprotein Sensor: A Piezoelectric Quartz Crystal Device", Int. J. Appl. Biomed. Eng.2, 1(2009)
[1-43] B. H. Weiller, L. Ceriotti, T. Shibata, D. Rein, M. A. Roberts, J. Lichtenberg, J. B. German, N. F. de Rooij, and E. Verpoorte, "analysis of lipoproteins by capillary zone electrophoresis in microfluidic devices: assay development and surface roughness measurements", Anal. Chem. 74, 1702-1711. (2002)
[1-44] G. Zhuang, Q. Jin, J. Liu, H. Cong, K. Liu, J. Zhao, M. Yang, H. Wang, "A low temperature bonding of quartz microfluidic chip for serum lipoproteins analysis", Biomed. Microdevices 8, 255-261. (2006)
[1-45] S.G. Anaokar, G.L. Antonopoulos, N. Muchnik, "method for determining HDL concentration from whole blood or plasma", United States Patent, US 7087397 B2(2006)
[1-46]Y.B. Thakore, "Direct measurement of HDL cholesterol via dry chemistry", United States Patent, US 5135716(1992)
[1-47]M.B. Kozak, A. Badke, "Method of assaying whole blood for HDL cholesterol", United States Patent, US 5460974(1995)
[1-48]J.E. Kim, J.H. Cho, S.H. Paek, "Functional membrane-implanted lab-on-a-chip for analysis of percent HDL cholesterol", Anal. Chem., 77, 7901-7907(2005)
[1-49]M. Elizabeth, B.J. Morton, L.L. Wong, “HDL Cholesterol Sensor”, International Patent, WO2006/067424(2006)
[1-50] S. H. Paek, M. R. Jang, R. S. Mok, S. C. Kim, H. B. Kim, "Immunochromatographic membrane strip assay system for a single-class plasma lipoprotein cholesterol, exemplified by high-density lipoprotein cholesterol measurement", Biotechnol. Bioeng. 62, 2, 145-154. (1999)
[1-51] M. I. Mackness, and P. N. Durrington. "Lipoprotein separation and analysis for clinical studies", In C. A. Converse, and E. R. Skinner (ed.), Lipoprotein analysis: a practical approach. (1992)
[1-52] F. Reinitzer, "Contribution to the understanding of cholesterol", Monatshefte Fur Chemie(wien) 9, 41-421. (1888)
[1-53] P. S. Drzaic, "Polymer dispersed nematic liquid crystal for large area displays and light valves", J. Appl. Phys. 60, 2142-48. (1986)
[1-54] Y. H. Lin, H. Ren, S. T. Wu, "High contrast polymer-dispersed liquid crystal in a 90° twisted cell", Appl. Phys. Lett. 84, 4083. (2004)
[1-55] H. Ren, Y. H. Fan, Y. H. Lin,and S. T. Wu, "Tunable-focus microlens arrays using nanosized polymer-dispersed liquid crystal droplets", Opt. Commun. 247(1-3), 101-106. (2005)
[1-56] P. J. Collings, M. Hird, "Introduction to Liquid Crystals: Chemistry and Physics", Liquid Crysyal Today 7, 3. (1997)
[1-57] Y. H. Lin, J. K. Li, T. Y. Chu, and H. K. Hsu, "A bistable polarizer-free electro-optical switch using a droplet manipulation on a liquid crystal and polymer composite film", Optics Express, 18, 10, 10104-10111. (2010)
[1-58] 陳俊霖, ”一種利用一低於液晶聚合物薄膜上運動的不需偏振片之反射式顯示器”,國立交通大學, 碩士論文(民國 99 年 6 月)
[1-59] 朱庭玉. “液晶聚合物薄膜之表面物理研究及應用開發”, 國立交通大學, 碩士論文(民國 100 年 6 月)
[1-60] Y. H. Lin, T. Y. Chu, W. L. Chu, Y. S. Tsou, Y. P. Chiu, F. Lu, W. C. Tsai, and S. T. Wu, "A Sperm Testing Device on a Liquid Crystal and Polymer Composite Film", J. Nanomedic Nanotechnol, 9:001 (2011)
[2-1] T. Young, "Miscellaneous Works", G. Peacock, ed. (Murray, London), 418. (1885)
[2-2] P. S. de Laplace, "Mechanique Celeste", Supplement to Book, Paris: Durat, 10. (1806)
[2-3] W. D. Harkins, "Surface energy and the orientation of molecules in surfaces as revealed by surface energy relations", J. Phys. Chem. 139, 647. (1928)
[2-4] K. Kendall, "Adhesion: Molecules and Mechanics", Science, 263, 5154, 1720-25. (1994)
[2-5] A. Dupre, "Theorie Mecanique de la Chaleur", Gauthier-Villars, Paris, 368. (1869)
[2-6] L. A. Girifalco, R. J. Good, "A Theory for the Estimation of Surface and Interfacial Energies. I. Derivation and Application to Interfacial Tension", J. Phys. Chem. 61, 904. (1957)
[2-7] R. J. Good, "Contact angle, wetting, and adhesion: a critical review" J. Adhes. Sci. Technol, 6, 12. (1992)
[2-8] P. G. de Genns, "Wetting: statics and dynamics", Rev. Mod. Phys. 57, 3, 827–863. (1985)
[2-9] A. Mielke, T. Roubicek, "A Rate-Independent Model for Inelastic Behavior of Shape-Memory Alloys". Multiscale Model. Simul. 1 (4): 571–597(2003)
[2-10] C. W. Extrand, "Contact Angles and Hysteresis on Surfaces with Chemically Heterogeneous Islands", Langmuir, 19, 9, 3793–96. (2003)
[2-11] C. W. Extrand ,"Criteria for Ultralyophobic Surfaces", Langmuir, 20, 12, 5013–18. (2004)
[2-12] C. W. Extrand, "Model for Contact Angles and Hysteresis on Rough and Ultraphobic Surfaces", Langmuir, 18, 21, 7991–99. (2002)
[2-13] Y. L. Chen, C. A. Helm, J. N. Israelachvili, "Molecular mechanisms associated with adhesion and contact angle hysteresis of monolayer surfaces", J. Phys. Chem. 95, 26, 10736–747. (1991)
[2-14] L. Gao, T. J. McCarthy, "Contact Angle Hysteresis Explained", Langmuir, 19(22), 14, 6234–37. (2006)
[3-1] E.Y. Arashiro, N.R. Demarquette, "Use of the Pendant Drop Method to Measure Interfacial Tension between Molten Polymers", Mat. Res., 2, 1, 23-32(1999)
[3-2] C.E. Stauffer, "The Measurement of Surface Tension by the Pendant Drop Technique", J. Phys. Chem., 69(6), 1933-1938(1965)
[5-1] N. Fournier, V. Atgerb, A. Cogny, B. Vedie, P. Giral, A. Simond, N. Moattia, J.-L. Paul, "Analysis of the relationship between triglyceridemia and HDL-phospholipid concentrations: consequences on the efflux capacity of serum in the Fu5AH system", Atherosclerosis 157, 2, 315-323. (2001)
連結至畢業學校之論文網頁點我開啟連結
註: 此連結為研究生畢業學校所提供,不一定有電子全文可供下載,若連結有誤,請點選上方之〝勘誤回報〞功能,我們會盡快修正,謝謝!
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊