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研究生:鄭惠芳
研究生(外文):Huie-Fang Cheng
論文名稱:培養基配方和種植密度影響蝴蝶蘭商業子瓶苗生長及繼代培養與馴化週數和竹炭混合介質對出瓶苗生育之表現
論文名稱(外文):Medium formula and planting density affect the growth performance of commercial plantlet in vitro, and duration of subculture and acclimatization and bamboo charcoal mixture on the growth of de-flask seeding ex vitro in Phalaenopsis
指導教授:黃光亮黃光亮引用關係
指導教授(外文):Kuang-Liang Huang
學位類別:碩士
校院名稱:國立嘉義大學
系所名稱:園藝學系研究所
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
畢業學年度:100
語文別:中文
論文頁數:82
中文關鍵詞:蝴蝶蘭培養基配方種植密度馴化竹炭
外文關鍵詞:Phalaenopsismedium formulaplanting densityacclimatizationbamboo charcoal
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本研究旨在探討培養基配方與子瓶苗定植密度、繼代培養期、馴化週數及出瓶苗水苔添加竹炭對苗株品質之影響。
蝴蝶蘭Phalaenopsis Fortunne Saltzman ‘256’及Doritaenopsis Queen Beer ‘101’兩品種經花梗芽切分生苗,1/2MS及花寶一號基礎培養基,皆添0.1 mg•L-1 BA、 0.5 mg•L-1 NAA,子瓶階段以不同定植密度,調查繼代培養後十六週生長表現,結果顯示Phal. Fortunne Saltzman ‘256’及Dtps. Queen Beer ‘101’ 1/2 MS培養基較花寶一號培養基種植有較多葉片數;且在每瓶種植11或13株低密度時有較多之發根數5.09及4.33;Dtps. Queen Beer ‘101’每瓶種植13株時亦有較長平均根長2.23 cm及乾重0.17 g 。Dtps. Jiubao Red Rose ‘280’在1/2 MS培養基中隨種植密度降低而增加葉片數及根數,低密度時平均根數為2.90,葉片數為3.42,每瓶種植17或19株高密度時,平均根數為2.19,葉片數為2.83。顯示為提升瓶苗品質及增加芽切苗發根數,可使用1/2MS培養基,且每瓶培養定植株數為13株。
Phal. Fortunne Saltzman ‘256’、Dtps. Queen Beer ‘101’及Dtps. Jiuhbao Red Rose ‘280’子瓶苗繼代培養 10、12、14、16及18週再經馴化1、2、3週後出瓶種植於水苔介質中16週。結果顯示苗株地上部的生長量,明顯隨繼代培養時間增加遞增,地下部的生長量則以培養14週馴化2週的處理有最多之根數。品種間較佳之處理結果分別為Phal. Fortunne Saltzman ‘256’繼代培養14週與馴化2週;Dtps. Queen Beer ‘101’繼代培養16週與馴化2週;而Dtps. Jiuhbao Red Rose ‘280’則為繼代培養14週與馴化1週之處理。
Phal. Fortunne Saltzman ‘256’、Dtps. Queen Beer ‘101’及Dtps. Jiuhbao Red Rose ‘280’,以培養16週的子瓶苗為材料,經馴化3週出瓶種植。在水苔分別添加0%、10%、20%及40%顆粒大小3- 5 mm的竹炭之混合介質中,經16週栽培期調查苗株生長情形,顯示水苔添加10%竹炭的處理有最佳的生長表現,且隨竹炭添加量增加植株的存活率下降,顯示較高的竹炭添加量不利於植株生長,故如欲於水苔中添加竹炭作為介質使用,竹炭以不超過10%為宜。

This study mainly focus on the effect of medium formulas and culture density on the growth of Phalaenopsis plantlets in vitro. Phalaenopsis plantlets were transferred to commercial flask and treated with various duration of subculture and acclimatization, and to evaluate the deflask plantlets growth on mixed with sphagnum and bamboo charcoal.
The plantlets were cultured on 1/2 MS and Hyponex medium which from flower stalk of Phal. Fortunne Saltzman ‘256’ and Dtps. Queen Beer ‘101’, and only Dtps. Jiubao Red Rose ‘280’ cultured on 1/2MS medium. All the culture medium was supplemented 0.5 mg•L-1 NAA with 0.1 mg•L-1 BA. And the density were 11, 13, 15, 17 and 19 plantlets per flask. After 16-week culture, leaf number of Phal. Fortunne Saltzman ‘256’ and Dtps. Queen Beer ‘101’ cultured on 1/2MS medium is more than Hyponex medium, and the better root number of Phal. Fortunne Saltzman ‘256’and Dtps. Queen Beer ‘101’ are 5.09 and 4.33 respectively, at lower culture density of 11, 13 plantlets per flask. The best root length and dry weight of Dtps. Queen Beer ‘101’ are 2.23 cm and 0.17 g respectively, was also found at lower density. The leaf number and root number will increase when the culture density is decreasing. When plantlets culture with lower and higher culture density of 17,19 plantlets per flask , the average root numbers and leaf number are 2.9, 2.19 and 3.42, 2.83, respectively. The suggestion for increasing plant quality and rooting number is culture on 1/2MS medium with 13 plantlets per flask.
Phalaenopsis plantlets were transferred to commercial flask and plant in different culture (10, 12, 14, 16, 18 weeks) with different acclimatization weeks (1, 2, 3 weeks) then cultured in sphagnum. After cultured in sphagnum, the upper part of growth plantlets of traits increase with commercial flask culture time, and had the most root number on commercial flask 14 weeks with 2 acclimatization weeks. The best response on Phal. Fortunne Saltzman ‘256’ was found at acclimatization 2 weeks after 14 weeks of culture, on Dtps. Queen Beer ‘101’ was found at acclimatization 2 and 1 weeks after 16 and 18 weeks of culture, and on Dtps. Jiubao Red Rose ‘280’ was found at acclimatization 1 week after 14 weeks of culture.
Phalaenopsis plantlets were transferred to commercial flask and cultured 16 weeks with acclimatization 3 weeks then cultured in bamboo charcoal mixed with sphagnum moss ex vitro for 16 weeks. After cultured in bamboo charcoal mixed (0%, 10%, 20%, and 40%) with sphagnum moss, the best response on Phal. Fortunne Saltzman ‘256’ was found at apply 0%, 10% bamboo charcoal in sphagnum moss, it had the most leaf number, leaf span, leaf length and leaf width. And after four month, the best plantlets growth of traits at mixed 10% bamboo charcoal in sphagnum moss, the survival rate decreased with higher bamboo charcoal percent, and had yellowing root. Phalaenopsis plantlets cultured in bamboo charcoal mixed (10%) with sphagnum moss is recommended.
目錄 (Content)
壹、前言 (Introduction)……………………………………………1
貳、前人研究 (Literature review)…………………………………3
一、蝴蝶蘭與朵麗蝶蘭的分生繁殖…………………………………3
(一) 培養基組成分………………………………………………………3
(二) 培植體來源 ………………………………………………………4
1.頂端分生組織誘導癒傷組織及擬原球體形成……………4
2.葉片誘導癒傷組織及擬原球體形成………………………4
3.花梗節間及根尖誘導癒傷組織及擬原球體形成…………5
4.芽體增殖與變異……………………………………………5
(三) 瓶內培養時間與出瓶後光馴化對瓶苗生長的影響……………6
二、蘭科植物之水苔介質與特性………………………………………7
三、竹炭的特性…………………………………………………………8
参、材料與方法 (Materials and Methods)…………………………10
一、植物材料……………………………………………………………10
二、試驗場所……………………………………………………………10
三、試驗方法…………………………………………………………………………11
(一) 子瓶苗定瓶密度對芽切苗定瓶時生長的影響…………………11
(二) 培養時間對植株與馴化週數對出瓶後苗株生長之影響………11
(三) 水苔添加竹炭作為栽培介質對出瓶苗株生長之影響…………12
四、調查項目與方法……………………………………………………13
五、試驗設計與統計分析………………………………………………13
肆、結果 ( Results )…………………………………………………14
一、子瓶苗定瓶密度對芽切苗株生長的影響…………………………14
(一) 培養基種類與種植密度對葉片數目的響………………………14
(二) 培養基種類與種植密度對葉幅、葉長、葉寬影響……………14
(三) 培養基種類與種植密度對根數、總根長和平均根長的影響…15
(四) 葉綠素指標值(SPAD value) ……………………………………16
(五) 培養基種類及種植密度對植株鮮重、乾重的影響……………17
二、子瓶苗培養時間與馴化週數對出瓶後苗株生長之影響…………18
(一) Phalaenopsis Fortunne Saltzman‘256’的各項性狀調查結果…………………………………………………………………………18
1.葉片數…………………………………………………………………18
2.葉幅……………………………………………………………………19
3.葉長……………………………………………………………………20
4.葉寬……………………………………………………………………20
5.根部……………………………………………………………………21
6.存活率…………………………………………………………………22
(二) Doritaenopsis Queen Beer‘101’的各項性狀調查結果……22
1.葉片數…………………………………………………………………22
2.葉幅……………………………………………………………………23
3.葉長……………………………………………………………………24
4.葉寬……………………………………………………………………25
5.根部……………………………………………………………………25
6.存活率…………………………………………………………………26
(三) Doritaenopsis Jiuhao Red Rose‘280’的各項性狀調查結果…………………………………………………………………………26
1.葉片數…………………………………………………………………26
2.葉幅……………………………………………………………………27
3.葉長……………………………………………………………………28
4.葉寬……………………………………………………………………28
5.根部……………………………………………………………………29
6.存活率…………………………………………………………………30
三、水苔添加竹炭作為栽培介質對出瓶苗株生長之影響……..…….….......….34
(一) Phalaenopsis Fortunne Saltzman‘256’的各項性狀調查…………………………………………………………………………35
1.葉片數…………………………………………………………………35
2.葉幅……………………………………………………………………35
3.葉長……………………………………………………………………35
4.葉寬……………………………………………………………………35
5.根部……………………………………………………………………35
6.存活率…………………………………………………………………36
(二) Doritaenopsis Queen Beer‘101’的各項性狀調查…………………………………………………………………………36
1.葉片數…………………………………………………………………36
2.葉幅……………………………………………………………………36
3.葉長……………………………………………………………………36
4.葉寬……………………………………………………………………37
5.根部……………………………………………………………………37
6.存活率…………………………………………………………………37
(三) Doritaenopsis Jiuhbao Red Rose‘280’的各項性狀調查…………………………………………………………………………37
1.葉片數…………………………………………………………………37
2.葉幅……………………………………………………………………37
3.葉長……………………………………………………………………38
4.葉寬……………………………………………………………………38
5.根部……………………………………………………………………38
6.存活率…………………………………………………………………38
伍、討論 (Discussion)………………………………………………68
一、子瓶苗定瓶密度對芽切苗株生長之影響…………………………68
二、子瓶苗培養時間與馴化週數對出瓶後苗株生長之影響…………69
三、水苔添加竹炭作為栽培介質對出瓶苗株生長之影響……………72
陸、參考文獻 (Reference)……………………………………………74

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