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研究生:許博詠
研究生(外文):Bo-Yong Syu
論文名稱:束形真葉珊瑚與圓管星珊瑚超氧歧化酶基因選殖之研究
論文名稱(外文):Molecular cloning and characterization of superoxide dismutase from the scleractina corals, Euphyllia glabrescens and Tubastraea aurea
指導教授:郭傑民
指導教授(外文):Jimmy Kuo
學位類別:碩士
校院名稱:國立東華大學
系所名稱:海洋生物科技研究所
學門:自然科學學門
學類:海洋科學學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:72
中文關鍵詞:束形真葉珊瑚圓管星珊瑚共生藻超氧歧化酶基因選殖
外文關鍵詞:Euphyllia glabrescensTubastraea aureaSymbiodiumsuperoxide dismutaseCloning
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本研究目的為從束形真葉珊瑚、束形真葉珊瑚之共生藻及圓管星珊瑚選殖出各型超氧歧化酶(superoxide dismutase, SOD)基因。利用聚合酶鏈鎖反應(PCR)與RACE (rapid amplification of cDNA ends)技術進行基因選殖。自束形真葉珊瑚選殖出銅鋅型SOD (CuZnSOD)及粒線體錳型 SOD (mtMnSOD)基因全長序列,圓管星珊瑚選殖出銅鋅型SOD (CuZnSOD)及粒線體錳型SOD (mtMnSOD)之部分序列,束形真葉珊瑚之共生藻選殖出錳型SOD (MnSOD)與鐵型SOD (FeSOD)之部分序列。束形真葉珊瑚CuZnSOD基因全長為896 bp,可轉譯讀窗(open reading frame)蛋白質長度為206個胺基酸,並發現與海葵Anemonia viridis (AAS98800)相同性(identity)有52%。束形真葉珊瑚mtMnSOD基因全長為1096 bp,可轉譯讀窗(open reading frame)蛋白質長度為223個胺基酸,含有兩個可能的醣基結合位NHS和NLS,且與萼柱珊瑚Stylophila pistillata (AAX99423)相同性有86%。
The purpose of this study was cloned different types of superoxide dismutase (SOD) genes from Euphyllia glabrescens, symbiodium and Tubastraea aurea. We use polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) for gene cloning,were isolated copper-zinc SOD (CuZnSOD) and mitochondrial manganese SOD (mtMnSOD) of full-lengths from E. glabrescens, CuZnSOD and mtMnSOD of the partial sequence from T. aurea, manganese SOD (MnSOD) and iron SOD (FeSOD) of the partial sequence from symbiodium. The E. glabrescens CuZnSOD gene length of 896 bp and open reading frames encoded 206 amino acids. Deduced peptide was molecular mass of 22kDa; and we found that the sea anemone (Anemonia viridis, AAS98800) identity with 52%. The E. glabrescens mtMnSOD gene length of 1096 bp and open reading frames encoded 223 amino acids; and contains two potential glycosylation binding NHS and NLS. Deduced peptide was molecular mass of 24kDa; the coral (Stylophila pistillata, AAX99423) identity with 86%.
摘要…………………………………………………………………… I
Abstract…………………………………………………………………… II
目錄………………………………………………………………………… III
圖目錄………………………………………………………………… IV
表目錄………………………………………………………………… VI
附錄………………………………………………………………… VII
第一章 前言……………………………………………………………… 1
第二章 材料與方法……………………………………………………… 5
第三章 結果……………………………………………………………… 21
第四章 討論……………………………………………………………… 29
第五章 結論……………………………………………………………… 31
參考文獻…………………………………………………………………… 33
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