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研究生:洪堯彰
研究生(外文):Hong, Yaozhang
論文名稱:維生素D 代謝物對雞隻鈣離子恆定相關基因表現及25-羥化酶基因選殖
論文名稱(外文):Different VitD Metabolites On Calcium Homeostasis Related Gene Expression In Chickens And Cloning The 25-Hydroxylase Gene.
指導教授:鄭永祥鄭永祥引用關係
指導教授(外文):Cheng, Yeonghsiang
口試委員:鍾道強孫舜國溫秋明許惠貞鄭永祥
口試日期:2012-07-04
學位類別:碩士
校院名稱:國立宜蘭大學
系所名稱:生物技術研究所碩士班
學門:農業科學學門
學類:畜牧學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:85
中文關鍵詞:25-羥化酶選殖及表現、維生素D3 代謝物、生長性能
外文關鍵詞:25-hydroxylase, cloning and expression, Vitamin D metabolites, Performance
相關次數:
  • 被引用被引用:2
  • 點閱點閱:180
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  • 下載下載:14
  • 收藏至我的研究室書目清單書目收藏:0
維生素D3 是維持鈣離子恆定、動物骨骼發育與維持的要角,若缺乏會使鈣離子失
調造成動物脛骨軟骨不全症 (tibia dyschondroplasia TD) 或佝僂症 (rickets)。本論文利用
不同維生素D3 代謝物探討對雞隻鈣離子恆定相關基因表現。試驗設計是以48 隻1 日齡
肉雞隨機分配成四組,給予基礎日糧1 週後斷食1 天,再給予不同維生素D3 代謝物
(1α-D3 5μg/kg , 25-D3 69μg/kg, 1,25-D3 5μg/kg),結果顯示肉雞給予不同維生素D3 代謝物
時,25-D3 及1α-D3 皆上調calbindin 基因表現量。維生素D3 代謝物餵飼後6 小時後,
表現量顯著提高3 倍, 12 小時後約為2 倍;TRPV6 及β-glucuronidase 基因表現量在6
小時後皆有增加趨勢,但12 小時後表現量上調至少2 倍以上;Na/Pi IIb 基因表現量則
在6 小時和12 小時後提升5 倍。此外,本論文再以48 隻1 日齡肉雞隨機分配成四組,
連續3 週給予不同維生素D3 代謝物 (1α-D3, 25-D3, 1,25-D3) 為探討長期餵飼維生素D3
代謝物對雞隻生長性能影響,分析結果顯示不同維生素D3 代謝物對肉雞生長性能,骨
破裂強度均無顯著差異,但添加組均有提升趨勢。
種雞及蛋雞代謝因遺傳改進,飼養管理及飼料配方不斷改進下,造成肝臟代謝
25-hydroxylase 異常,影響維生素D3 轉化成活性型態,故本論文進行25-hydroxylase 基
因選殖及表現,期許藉由飼料添加給予, 改善維生素D3 利用效率。本試驗將
25-hydroxylase 構築於pPICZαA,並藉由P.pastoris 表現。西方墨點法結果顯示,
25-hydroxylase 分子量約為67kDa,並將維生素D3 與25-hydroxylase 共同培養再以
HPLC 證實生物活性,出現滯留時間在4.7 分鐘。總結,維生素D3 代謝物能在短時間內
調節鈣離子恆定相關基因表現,且連續餵飼3 週不同維生素D3 代謝物對肉雞生長性能
有提升趨勢,此外25-hydroxylase 已成功選殖及表現,再以維生素D3 共同培養後利用
HPLC 偵測,證實25-hydroxylase 經選殖後具有轉化維生素D3 成為25-hydroxyvitamin D3
之生物活性。
The result of continuous innovation in the field of genetics, management, nutrition is the
production of a high yielding breeders and hens, it results in 25-hydroxylase metabolic
abnormalities in liver, and affect vitamin D3 transform into the active form. The purpose of
this study was tried to cloning and expression 25-hydroxylase gene, and we expect to addition
to the feed would improve the vitamin D3 efficiency. 25-hydroxylase gene was constructed to
pPICZαA vector and expression by P.pastoris in this study. Results showed 25-hydroxylase
molecular weight approximately 67kDa by western blot, and we incubated vitamin D3 with 25-hydroxylase enzyme to confirm the bioactivity by HPLC, and observe the retention time at
4.7 minutes. Summarized, current results indicated vitamin D3 metabolites showing strong
up-regulation in short-term manner in calcium homeostasis related gene, and results in a
better tendency of growth performance. Furthermore, 25-hydroxylase gene was cloned and
expressed successfully, and 25-hydroxylase bioactivity was confirmed by HPLC assays.
目錄
摘要..........................................................................................................Ⅰ
目錄..........................................................................................................IV
圖目錄......................................................................................................VIII
表目錄.........................................................................................................X
壹、前言........................................................................................................1
貳、文獻檢討....................................................................................................2
一、鈣離子對動物生理的重要性......................................................................................2
二、維生素D3的代謝及生理功能......................................................................................3
三、維生素D3 調節鈣離子吸收之分子機制..............................................................................4
四、荷爾蒙及標的蛋白調節鈣離子吸收.................................................................................5
五、維生素D3代謝物於畜禽之應用及體內調節的重要性....................................................................9
六、維生素D3 代謝物對生物體免疫抗菌機制...........................................................................11
七、維生素D3代謝物與蛋殼品質.....................................................................................12
參、研究目的...................................................................................................15
肆、實驗材料與儀器..............................................................................................16
第一章、白肉雞飼料添加不同維生素D3代謝物試驗.......................................................................20
試驗一、維生素D3 代謝物對家禽鈣離子恆定相關基因研究.................................................................20
一、前言.......................................................................................................20
二、材料及方法.................................................................................................20
1. 試驗動物與處理..............................................................................................20
2. mRNA 表現測量...............................................................................................22
3. 血鈣濃度測定................................................................................................22
三、統計分析...................................................................................................23
四、結果與討論.................................................................................................23
1. 維生素D3 代謝物對血鈣之影響...................................................................................23
2. 維生素D3代謝物對calbindin 基因表現...........................................................................24
3. 維生素D3 代謝物對TRPV6 及β-glucuronidase 基因表現之影響.......................................................25
4. 維生素D3 代謝物對24-hydroxylase 基因表現之影響................................................................25
5. 維生素D3 代謝物對VDR 基因表現之影響...........................................................................26
6. 維生素D3代謝物對Na/PiIIb 基因表現之影響.......................................................................26
試驗二、 維生素D3代謝物對家禽生長性能之研究........................................................................28
一、前言.......................................................................................................28
二、材料及方法.................................................................................................28
1. 動物試驗與處理 .............................................................................................28
2. 脛骨鈣、磷含量分析...........................................................................................28
3. 脛骨破裂強度分析.............................................................................................28
三、統計分析...................................................................................................29
四、結果與討論.................................................................................................29
1. 維生素D3 代謝物對白肉雞生長性能表現之影響..................................................................... 30
2. 維生素D3 代謝物對白肉雞脛骨鈣、磷含量及破裂強度之影響............................................................30
第一章、25-hydroxylase 基因選殖及表現之研究......................................................................33
一、前言.......................................................................................................33
二、材料及方法.................................................................................................33
1. 基因構築...................................................................................................33
(1). 基因序列的設計............................................................................................33
(2). 表現載體抽取..............................................................................................33
(3). 接合作用 (Ligation) ......................................................................................34
(4). 大腸桿菌勝任細胞 (Competent Cell) 的製備 ...................................................................34
(5). 轉型作用 (Transformation) ................................................................................35
(6). 質體抽取 (Plasmid DNA Extraction) ........................................................................35
(7). 重組質體基因鑑定...........................................................................................35
a. 限制酶切割確認 .............................................................................................35
b. PCR鑑定....................................................................................................36
c. DNA定序 (DNA sequencing)...................................................................................36
2. 重組質體於真核系統的表現......................................................................................36
(1). 酵母菌勝任細胞的製備.......................................................................................36
(2). 重組質體電穿孔轉殖 (Electroporation) ......................................................................37
(3). 重組基因的表現............................................................................................37
3. 重組酵母菌表現產物細胞內與細胞外分析...........................................................................38
(1). 細胞外蛋白質濃縮..........................................................................................38
(2). SDS-PAGE蛋白質電泳分析....................................................................................38
(3). Western Blot抗體標定分析..................................................................................39
4、活性分析....................................................................................................40
三、結果與討論.................................................................................................41
1. 25-hydroxylase 基因選殖與表現...............................................................................41
2. 25-hydroxylase 活性分析.....................................................................................42
伍、結論.......................................................................................................43
陸、實驗數據圖表................................................................................................44
柒、參考文獻...................................................................................................59
冯海婷、陆凌霄、文鹏、陆群.2010. 微生物转化制备活性维生素D3 的研究进展。2010
年第4 期:55-58。
行政院農業委員會。 畜牧類農情概況分析。2010。
許育誠、楊昆達。2010。間歇性低氧影響心肌細胞鈣離子恆定機制之探討。慈濟大學
生理暨解剖醫學研究所碩士論文。
儿科药学杂志2010 年第16 卷第3 期Journal of Pediatric Pharmacy 2010,Vol. 16,
No. 3:41-43。
Abe, E., Horikawa, H., Masumura, T., Sugahara. M., Kubota, M., Suda, T., 1982. Disorders of
cholecalciferol metabolism in old egg-laying hens. J. Nutr. 112: 436-446.
Akerström, G., Hellman, P., Hessman, O., Segersten, U., Westin, G., 2005. Parathyroid glands
in calcium regulation and human disease. Ann. N.Y. Acad. Sci. 1040: 53-58.
Atencio, A., Pesti, GM., Edwards, H. M. Jr., 2005a. Twenty-five hydroxycholecalciferol as a
cholecalciferol substitute in broiler breeder hen diets and its effect on the performance
and general health of the progeny. Poult Sci. 84: 1277-1285.
Atencio, A., Edwards, H. M. Jr., Pesti, G. M., 2005b. Effect of the level of cholecalciferol
supplementation of broiler breeder hen diets on the performance and bone abnormalities
of the progeny fed diets containing various levels of calcium or
25-hydroxycholecalciferol. Poult Sci. 84: 1593-1603.
Bernstein, R. S., Nevalainen, T., Schraer, R., Schraer, H., 1968. Intracellular distribution and
role of carbonic anhydrase in the avian (Gallus domesticus) shell gland mucosa. Biochim
Biophys Acta. 159(2): 367-376.
Bar, A., and Hurwitz, S., 1973. Uterine calcium-binding protein in the laying fowl. Comp
Biochem Physiol A Comp Physiol. 45(2): 579-586.
Bar, A., and Hurwit, S., 1987. Vitamin D metabolism and calbindin (calcium-binding protein)
in aged laying hens. J. Nutr. 117(10): 1775-1779.
Brown, A. J., Zhong, M., Finch, J., Ritter, C., Slatopolsky, E., 1995. The roles of calcium and
1,25-dihydroxyvitamin D3 in the regulation of vitamin D receptor expression by rat
parathyroid glands. Endocrinology. 136(4): 1419-1425.
Bar, A., Vax, E., Hunziker, W., Halevy, O., Striem, S., 1996. The role of gonadal hormones in
gene expression of calbindin (Mr 28,000) in the laying hen. Gen Comp Endocrinol.
103(1): 115-122.
Berridge, M. J., 1998. Neuronal calcium signaling. Neuron. 21: 13-26
Brenza, H. L., and DeLuca, H. F., 2000. Regulation of 25-hydroxyvitamin D3 1α
-hydroxylase gene expression by parathyroid hormone and 1,25-dihydroxyvitamin D3.
Arch Biochem Biophys. 381: 143-152.
Bar, A., Vax, E., Striem, S., 1999. Relationships among age, eggshell thickness and vitamin D
metabolism and its expression in the laying hen. Comp Biochem Physiol A Mol Integr
Physiol. 123(2): 147-154.
Bar, A., 2008. Calcium homeostasis and vitamin D metabolism and expression in strongly
calcifying laying birds. Comp Biochem Physiol A Mol Integr Physiol. Part A 151 :
477-490.
Balesaria, S., Sangha, S., Walters, J. R., 2009. Human duodenum responses to vitamin D
metabolites of TRPV6 and other genes involved in calcium absorption. Am J. Physiol
Gastrointest Liver Physiol. 297: G1193-G1197.
Corradino, R. A., Wasserman, R. H., Pubols, M. H., Chang SI. 1968. Vitamin D3 induction of
a calcium-binding protein in the uterus of the laying hen. Arch Biochem Biophys..125(1):
378-380.
Chambers, T. J., and Magnus, C. J., 1982. Calcitonin alters behaviour of isolated osteoclasts.
J. Pathol. 136: 27-39.
Clunies, M., Emslie, J., Leeson, S., 1992. Effect of dietary calcium level on medullary bone
calcium reserves and shell weight of Leghorn hens. Poult Sci. 71(8): 1348-1356.
Christakos, S., Raval-Pandya, M., Wernyj, R. P., Yang, W., 1996. Genomic mechanisms
involved in the pleiotropic actions of 1,25-dihydroxyvitamin D3. The Biochemical J. 316
(2): 361-371.
Christakos, S., and Liu, Y., 2004. Biological actions and mechanism of action of
calbindin in the process of apoptosis. J. Steroid Biochem. Mol. Biol. 89-90: 401-404.
Chang, Q., Hoefs, S., van der Kemp, A. W., Topala, C. N., Bindels, R. J., Hoenderop, J. G.,
2005. The β-glucuronidase klotho hydrolyzes and activates the TRPV5 channel. Sci. 310:
490-493.
Christakos, S., Liu, Y., Dhawan, P., Peng, X., 2005. The calbindins: calbindin D9k and
calbindin D28K. In Vitamin D, 2nd ed. Feldman, Pike and Glorieux, Eds. 42: 721-735.
Christakos, S., Dhawan, P., Benn, B., Porta, A., Hediger, M., Oh, G. T., Jeung, E. B., Zhong,
Y., Ajibade, D., Dhawan, K., Joshi, S., 2007. Vitamin D molecular mechanism of action.
N.Y. Acad. Sci. 1116: 340-348.
Chou, S. H., Chung, T. K., Yu, B., 2009. Effects of supplemental 25-hydroxycholecalciferol
on growth performance, small intestinal morphology, and immune response of broiler
chickens. Poult Sci. 88: 2333-2341.
Christakos, S., Dhawan, P., Ajibade, D., Benn, B. S., Feng, J., Joshi, S. S. 2010. Mechanisms
involved in vitamin D mediated intestinal calcium absorption and in non-classical actions
of vitamin D. J Steroid Biochem Mol Biol. 121: 183-187.
Christel, C., and Lee, A., 2012. Ca2+-dependent modulation of voltage-gated Ca2 + channels.
1820: 1243-1252.
Cui, M., Li, Q., Johnson, R., Fleet, J. C., 2012. Villin promoter-mediated transgenic
expression of TRPV6 increases intestinal calcium absorption in wild-type and VDR
knockout mice. JBMR. 1662.
de Boland, A. R., and.Norman, A., 1990a. Evidence for involve ment of protein kinase C and
cyclic adenosine, 3',5' monophosphate-dependent protein kinase in the
1,25-dihydroxy-vitamin D3-mediated rapid stimulation of intestinal calcium transport
(transcaltachia). Endocrinology. 127: 39-45.
de Boland, A. R., and Norman, A. W., 1990b. Influx of extracellular calcium mediates
1,25-dihydroxyvitamin D3-dependent transcaltachia (the rapid stimulation of duodenal
Ca2+ transport. Endocrinology. 127: 2475-2480.
DeLuca, H. F., 2004. Overview of general physiologic features and functions of vitamin D.
Am. J. Clin. Nutr. 80(6 Suppl): 1689S-1696S.
Dhawan, P., Peng, X., Sutton, A. L., MacDonald, P. N., Croniger, C. M., Trautwein, C.,
Centrella, M., McCarthy, T. L., Christakos, S., 2005. Functional cooperation between
CCAAT/enhancer-binding proteins and the vitamin D receptor in regulation of
25-hydroxyvitamin D3 24-hydroxylase. Mol Cell Biol. 25(1): 472-487.
Dai, X., Sayama, K., Tohyama, M., Shirakata, Y., Hanakawa, Y., Tokumaru, S., Yang, L.,
Hirakawa, S., Hashimoto, K., 2010. PPARγ mediates innate immunity by regulating the
1α,25-dihydroxyvitamin D3 induced hBD-3 and cathelicidin in human keratinocytes. J.
Dermatol Sci. 60: 179-186.
Ehrenspeck, G., Schraer, H., Schraer, R., 1971. Calcium transfer across isolated avian shell
gland. The American J. Physiology. 220(4): 967-972.
Eastin, W. C. Jr., and Spaziani, E., 1978. On the mechanism of calcium secretion in the avian
shell gland (uterus). Bio. Reproduction. 19(3): 505-518.
Erben, R. G., Scutt, A. M., Miao, D., Kollenkirchen, U., Haberey, M., 1997. Short-term
treatment of rats with high dose 1,25-dihydroxyvitamin D3 stimulates bone formation and
increases the number of osteoblast precursor cells in bone marrow. Endocrinology. 138:
4629-4635.
Fullmer, C. S., Brindak, M. E., Bar, A., Wasserman, R. H., 1976. The purification of
calcium-binding protein from the uterus of the laying hen. Proc Soc Exp Biol Med.
152(2): 237-241.
Ferrari, S., Bonjour, J. P., Rizzoli, R., 1998. The vitamin d receptor gene and calcium
metabolism. TEM. 9(7): 259-265.
Findlay, D. M., and Sexton, P. M., 2004. Calcitonin. Growth Factors. 22: 217-224.
Guerreiro, P. M., Renfro, J.L., Power, D. M., Canario, A. V., 2007. The parathyroid hormone
family of peptides: structure, tissue distribution, regulation, and potential functional roles
in calcium and phosphate balance in fish. Am. J. Physiol. Regul. Integr. Comp. Physiol.
292: 679-696.
Henry, H. L., 1979. Regulation of the hydroxylation of 25-hydroxyvitamin D3 in vivo and in
primary cultures of chick kidney cells. J. Bio Chem. 254(8): 2722-2729.
Henry, H. L., 1985. Parathyroid hormone modulation of 25-hydroxyvitamin D3 metabolism
by cultured chick kidney cells is mimicked and enhanced by forskolin. Endocrinology.
116(2): 503-510.
Hall, A. K., and Norman, A. W., 1990. Regulation of calbindin-D28K gene expression in the
chick intestine: effects of serum calcium status and 1,25-dihydroxyvitamin D. JBMR. 5:
331-336.
Haussler, M. R., Haussler, C. A., Jurutka, P. W., Thompson, P. D., Hsieh, J. C., Remus, L, S.,
Selznick, S. H., Whitfield, G. K., 1997. The vitamin D hormone and its nuclear receptor:
molecular actions and disease states. J. Endocrinol. 154: 57-73.
Hattenhauer, O., Traebert, M., Murer, H., Biber, J., 1999. Regulation of small intestinal Na-Pi
type IIb cotransporter by dietary phosphate intake. Am. J. Physiol. 277: 756-762.
Huff, G. R., Huff, W. E., Balog, J. M., Rath, N. C., Xie, H., Horst, R. L., 2002. Effect of
dietary supplementation with vitamin D metabolites in an experimental model of turkey
osteomyelitis complex. Poult Sci. 81: 958-965.
Huang, C.L., Sun, L., Moonga, B. S., Zaidi, M., 2006. Molecular physiology and
pharmacology of calcitonin. Cell Mol. Biol. 52: 33-43.
Han, J. C., Yang, X. D., Zhang, T., Li, H., Li, W. L., Zhang, Z. Y., Yao, J. H., 2009. Effects of
1α-hydroxycholecalciferol on growth performance, parameters of tibia and plasma, meat
quality, and type IIb sodium phosphate cotransporter gene expression of one- to
twenty-one-day-old broilers. Poult Sci. 88: 323-329.
Ishibe, M., Nojima, T., Ishibashi, T., Koda, T., Kaneda, K., Rosier, R. N., Puzas, J. E., 1995.
17 beta-estradiol increases the receptor number and modulates the action of
1,25-dihydroxyvitamin D3 in human osteosarcoma-derived osteoblast-like cells. Calcif
Tissue Int. 57(6): 430-435.
Jones, G., Prosser, D. E., Kaufmann, M., 2012. 25-Hydroxyvitamin D-24-hydroxylase
(CYP24A1): Its important role in the degradation of vitamin D. Arch. Biochem. Biophys.
523(1): 9-18.
Jiang, Y., and Fleet, J. C., 2012. Effect of phorbol 12-myristate 13-acetate activated
signaling pathways on 1α, 25 dihydroxyvitamin D3 regulated human
25-hydroxyvitamin D3 24-hydroxylase gene expression in differentiated Caco-2 cells. J.
Cell Biochem. 113(5): 1599-1607.
Kato, S., 2000. The function of vitamin D receptor in vitamin D action. J. Biochem. 127:
717-722.
Khanal, R. C., Peters, T. M., Smith, N. M., Nemere, I., 2008. Membrane receptor-initiated
signaling in 1,25(OH)2D3-stimulated calcium uptake in intestinal epithelial cells. J. Cell
Biochem. 105: 1109-1116.
Khoo, A. L., Chai, L. Y., Koenen, H. J., Oosting, M., Steinmeyer, A., Zuegel, U., Joosten, I.,
Netea, M. G., van der Ven, A. J. 2011. Vitamin D3 down-regulates proinflammatory
cytokine response to Mycobacterium tuberculosis through pattern recognition receptors
while inducing protective cathelicidin production. Cytokine. 55: 294-300.
Lee, S., Szlachetka, M., Christakos, S., 1991. Effect of glucocorticoids and
1,25-dihydroxyvitamin D3 on the developmental expression of the rat intestinal vitamin D
receptor gene. Endocrinology. 129(1): 396-401.
Lowe, K. E., Maiyar, A. C., Norman, A. W., 1992. Vitamin D-mediated gene expression.
Critical Reviews Eukaryot Gene Expr. 2(1): 65-109.
Lin, N. U., Malloy, P. J., Sakati, N., al-Ashwal, A., Feldman, D., 1996. A novel mutation in
the deoxyribonucleic acid-binding domain of the vitamin D receptor causes hereditary
1,25-dihydroxyvitamin D-resistant rickets. J. Clinical Endocrinology and Metabolism.
81(7): 2564-2569.
Liu, S. M., Koszewski, N., Lupez, M., Malluche, H. H., Olivera, A., Russell, J., 1996.
Characterization of a response element in the 5'-flanking region of the avian (chicken)
PTH gene that mediates negative regulation of gene transcription by
1,25-dihydroxyvitamin D3 and binds the vitamin D3 receptor. Baltimore, Md. 10(2):
206-215.
Liu, P. T., Stenger, S., Tang, D. H., Modlin, R. L., 2007. Cutting edge: Vitamin D-mediated
human antimicrobial activity against Mycobacterium tuberculosis is dependent on the
induction of cathelicidin. J. Immunology 179: 2060–2063.
Lee, G. S., Jung, E. M., Choi, K. C., Oh, G. T., Jeung, E. B., 2009. Compensatory induction of
the TRPV6 channel in a calbindin-D9k knockout mouse: its regulation by
1,25-hydroxyvitamin D3. J. Cell Biochem. 108: 1175-1183.
Liu, P. T., Schenk, M., Walker, V. P., Dempsey, P. W., Kanchanapoomi, M., Wheelwright, M.,
Vazirnia, A., Zhang, X., Steinmeyer, A., Zügel, U., Hollis, B. W., Cheng, G., Modlin, R.
L., 2009. Convergence of IL-1β and VDR activation pathways in human TLR2/1-induced
antimicrobial responses. PLoS ONE. 4(6): e5810.
Lauridsen, C., Halekoh, U., Larsen, T., Jensen, S. K., 2010. Reproductive performance and
bone status markers of gilts and lactating sows supplemented with two different forms of
vitamin D. J. Anim. Sci. 88: 202-213.
Landry, C. S., Ruppe, M. D., Grubbs, E. G., 2011. Vitamin D reseptors and parathyroid
glands. Endocr. Pract. 17[Suppl 1]: 63-68.
Mayel-Afshar, S., Lane, S. M., Lawson, D. E., 1988. Relationship between the levels of
calbindin synthesis and calbindin mRNA in chick intestine. J. Biol. Chem. 9: 4355-436.
Mangelsdorf, D. J., Evans, R.M., 1995. The RXR heterodimers and orphan receptors. Cell.
83(6): 841-850.
Munder, M., Herzberg, I. M., Zierold, C., Moss, V. E., Hanson, K., Clagett-Dame, M.,
DeLuca, H. F., 1995. Identification of the porcine intestinal accessory factor that enables
DNA sequence recognition by vitamin D receptor. Proc. Natl. Acad. Sci. USA. 28;92(7):
2795-2799.
Murayama, A., Takeyama, K., Kitanaka, S., Kodera, Y., Kawaguchi, Y., Hosoya, T., Kato,
S., 1999. Positive and negative regulations of the renal 25-hydroxyvitamin D3
1a-hydroxylase gene by parathyroid hormone, calcitonin, and 1α,25(OH)2D3 in intact
animals. Endocrinology. 140(5): 2224-2231.
Muscher, A., Breves, G., Huber, K., 2009. Modulation of apical Na/Pi cotransporter type
IIb expression in epithelial cells of goat mammary glands. J. Anim. Physiol. Anim.
Nutr. 93(4): 477-485.
Navickis, R. J., Katzenellenbogen, B. S., Nalbandov, A. V., 1979. Effects of the sex steroid
hormones and vitamin D3 on calcium-binding proteins in the chick shell gland. Biol.
Reprod. 21(5): 1153-1162.
Norman, A. W., Putkey, J. A., Nemere, I., 1982. Intestinal calcium transport: pleiotropic
effects mediated by vitamin D. Fed Proc. 41(1): 78-83.
Nys, Y., Parkes, C. O., Thomasset, M., 1986. Effects of suppression and resumption of shell
formation and parathyroid hormone on uterine calcium-binding protein, carbonic
anhydrase activity, and intestinal calcium absorption in hens. Gen Comp Endocrinol.
64(2): 293-299.
Nemere, I., and Norman, A. W., 1988. 1,25-dihydroxyvitamin D3-mediated vesicular
transport of calcium in intestine: Time course studies. Endocrinology. 122: 2962-2969.
Nemere, I., and Norman, A. W., 1990. Transcaltachia, vesicular calcium transport, and
microtubule-associated calbindin-D28K: Emerging views of 1,25-dihydroxyvitamin
D3-mediated intestinal calcium absorption. Miner Electrolyte Metab. 16: 109-114.
Nemere, I., and Norman, A. W., 1991. Redistribution of cathepsin B activity from the
endosomal-lysosomal pathway in chick intestine within 3 min of calcium absorption. Mol.
Cell Endocrinol. 78: 7-16.
Nelson, C. D., Nonnecke, B. J., Reinhardt, T. A., Waters, W. R., Beitz, D. C., Lippolis, J. D.,
2011. Regulation of mycobacterium-specific mononuclear cell responses by
25-hydroxyvitamin D3. PLoS ONE 6(6): e21674.
Portale, A. A., Halloran, B. P., Morris, R. C. Jr., 1989. Physiologic regulation of the serum
concentration of 1,25-dihydroxyvitamin D by phosphorus in normal men. J. Clin. Invest.
83(5): 1494-1499.
Potts, J. T., 2005. Parathyroid hormone: past and present. J. Endocrinol. 187(3): 311-325.
Potts, J. T., and Gardella, T. J., 2007. Progress, paradox, and potential: parathyroid hormone
research over five decades. Ann. N. Y. Acad. Sci. 1117: 196-208.
Pesti, G. M., and Shivaprasad, H. L., 2010. The influence of excessive levels of
1α-hydroxycholecalciferol on the growth and tissue appearance of market weight
chickens. J. Appl. Poult. Res. 19 : 349-353.
Reichel, H., Koeffler, H. P., Norman, A. W., 1989. The role of the vitamin D endocrine
system in health and disease. N Engl J Med. 320(15): 980-991.
Reinhardt, T. A., and Horst, R. L., 1990. Parathyroid hormone down-regulates
1,25-dihydroxyvitamin D receptors (VDR) and VDR messenger ribonucleic acid in vitro
and blocks homologous up-regulation of VDR in vivo. Endocrinology. 127(2): 942-948.
Ross, T. K., Moss, V. E, Prahl, J. M., DeLuca, H. F., 1992. A nuclear protein essential for
binding of rat 1,25-dihydroxyvitamin D3 receptor to its response elements. Proc Natl
Acad Sci. USA. 89(1): 256-560.
Rath, N. C., Kannan, L., Pillai, P. B., Huff, W. E., Huff, G. R, Horst, R. L., Emmert, J. L.,
2007. Evaluation of the efficacy of vitamin D3 or its metabolites on thiram-induced tibial
dyschondroplasia in chickens. Res. in Veterinary Sci. 83 : 244-250.
Schlüns, J., and Diamantstein, T., 1966. Behaviour of carbonic anhydrase in the uterus of
hens during moult. Nat. 209(5020): 304.
Snapir, N., and Perek, M., 1970. Distribution of calcium, carbonic anhydrase and alkaline
phosphatase activities in the uterus and isthmus of young and old white leghorn hens.
Poult Sci. 49(6): 1526-1531.
Strom, M., Sandgren, M. E, Brown, T. A, DeLuca, H. F., 1989. 1,25-Dihydroxyvitamin D3
up-regulates the 1,25-dihydroxyvitamin D3 receptor in vivo. Proc Natl Acad Sci.
USA.86(24): 9770-9773.
Sandgren, M. E., and DeLuca, H. F., 1990. Serum calcium and vitamin D regulate
1,25-dihydroxyvitamin D3 receptor concentration in rat kidney in vivo. Proc Natl Acad
Sci. USA. 87(11): 4312-4314.
Soares, J. H. Jr., Kerr, J. M,, Gray, R. W., 1995. 25-hydroxycholecalciferol in poultry
nutrition. Poult Sci. 74(12): 1919-1934.
Suda, T., Ueno, Y., Fujii, K., Shinki, T., 2002. Vitamin D and bone. J. Cell Biochem. 88:
259-266.
Stoffels, K., Overbergh, L., Giulietti, A., Verlinden, L., Bouillon, R., Mathieu, C., 2006.
Immune regulation of 25-hydroxyvitamin-D3-1α-hydroxylase in human monocytes.
JBMR. 21: 37-47.
Rana, S., Xia, Q., Miao, D., Hendy, G. N., Goltzman, D., 2008. Exogenous PTH and
endogenous 1,25-dihydroxyvitamin D are complementary in inducing an anabolic effect
on bone. JBMR. 23: 1257-1266.
Tanaka, Y., and DeLuca, H. F., 1984. Rat renal 25-hydroxyvitamin D3-1- and
24-hydroxylases: their in vivo regulation. Am J. Physiol. 246: E168-173.
Theofan, G., Nguyen, A. P., Norman, A. W., 1986. Regulation of calbindin-D28K gene
expression by 1,25-dihydroxyvitamin D3 is correlated to receptor occupancy. J. Biol
Chem. 36: 16943-16947.
Takeyama, K., and Kato, S., 2011. The vitamin D3 1alpha-hydroxylase gene and its regulation
by active vitamin D3. Biosci. Biotechnol. Biochem. 75(2): 100684-1-6.
Uhland-Smith, A., and DeLuca, H. F., 1993. The necessity for calcium for increased renal
vitamin D receptor in response to 1,25-dihydroxyvitamin D. Biochim Biophys Acta.
1176(3): 321-326.
Wasserman, R. H., and Taylor, A. N., 1966. Vitamin D3-induced calcium-binding protein in
chick intestinal mucosa. Sci. 152(3723): 791-793.
Wilson, P. W., and Lawson, D. E. M., 1980. Calcium binding activity by chick intestinal
brush-border membrane vesicles. Pflugers Arch. 389: 69-74.
Wasserman, R. H., Smith, C. A., Smith, C. M., Brindak, M. E., Fullmer, C. S., Krook, L.,
Penniston, J. T., Kumar, R., 1991. Immunohistochemical localization of a calcium pump
and calbindin-D28k in the oviduct of the laying hen. Histochemistry. 96(5): 413-418.
Wasserman, R. H., Chandler, J. S., Meyer, S. A, Smith, C. A., Brindak, M. E., Fullmer, C. S.,
Penniston, J. T., Kumar, R., 1992. Intestinal calcium transport and calcium extrusion
processes at the basolateral membrane. J. Nutr. 122(3): 662-671.
White, J. H., 2010. Vitamin D as an inducer of cathelicidin antimicrobial peptide expression:
past, present and future. J Steroid Biochem Mol Biol. 121: 234-238.
Witschi, A. K., Liesegang, A., Gebert, S., Weber, G. M., Wenk, C., 2011. Effect of source
and quantity of dietary vitamin D in maternal and creep diets on bone metabolism and
growth in piglets. J. Anim. Sci. 89: 1844-1852.
Xu, H., Bai, L., Collins, J. F., Ghishan, F. K., 2001. Age-dependent regulation of rat
intestinal type IIb sodium-phosphate cotransporter by 1,25-(OH)2 vitamin D3. Am J.
Physiol Cell Physiol. 282: 487-493.
Yan, F., 2007. Characterization of the chicken small intestine type IIb sodium phosphate
cotransporter. Poult Sci. 86: 67-76.
Zhong, Y., Armbrecht, H. J., and Christakos, S. 2009. Calcitonin, a regulator of the
25-hydroxyvitamin D3 1-hydroxylase gene. J. Biol Chem. 284(17): 11059-11069.
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