(34.229.64.28) 您好!臺灣時間:2021/05/06 05:15
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:蔡雅如
研究生(外文):Ya-Ju Tsai
論文名稱:點帶石斑生殖腺性別分化與性轉變探討
論文名稱(外文):The gonadal sex differentiation and sex change of orange-spotted grouper, Epinephelus coioides
指導教授:張清風張清風引用關係
指導教授(外文):Ching-Fong Chang
學位類別:博士
校院名稱:國立臺灣海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:188
中文關鍵詞:點帶石斑生殖腺性別分化性轉變免疫組織染色
外文關鍵詞:Epinephelus coioidesgonadal sex differentiationsex changeimmunohistochemistry stain
相關次數:
  • 被引用被引用:1
  • 點閱點閱:239
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
點帶石斑 (Epinephelus coioides) 生殖腺先發育為雌性,孵化後四月齡兩葉生殖腺的兩股分支逐漸併合形成卵巢空腔 (ovarian cavity, OC),於四至五月齡卵巢空腔 (OC) 的形成作為雌魚性別分化的依據。隨著雌性生殖配子發育、增生,六個半月至七月齡出現初級卵母細胞 (primary oocyte, PO),與卵原細胞 (oogonium, OG) 共同夾雜於卵巢薄板 (OL) 內,一直到一年七月齡出現皮質小粒卵細胞 (cortical alveolar, CA)。最早在兩年又二月齡 (2+2 -age) 出現內生性卵黃堆積的成熟卵細胞 (vitellogenic oocyte, VO)。實驗以環化酵素抑制物 (aromatase inhibiter, AI) 或雄性素 (methyltestosterone, MT) 投餵或埋植處理性別分化期間 (3~5個月齡)、1~2年齡與四年齡成魚誘導性別轉換。利用生殖腺環化酵素 (cyp19a1a)、dmrt1 (doublesex and mab-3-related transcription factor, number 1) 與性別分化相關基因 (sf1、dax1、foxL2、11β) 從基因表現,以及P450arom、Dmrt1、Pcna、Vasa組織免疫染色 (immunohistochemical stain, IHC) 切入探討點帶石斑的性別分化與性轉變。
點帶石斑diandric type雄魚的來源大部分自functional female性轉變而來 (secondary male),亦有少部分的自然性轉變由孵化後直接分化發育產生 (primary male),兩者皆可產生不同性轉變程度的精巢組織:a single spermatogenic cyst (SSC)、various stages of spermatogenic cyst (VSCs) 與mature stage of cysts (AMC)。自然性轉變 (primary male) 的精子生成包囊 (SSC, VSCs) 最早於一年半月齡出現。
點帶石斑cyp19a1a專一表現於7月齡生殖腺,cyp19a1a於性別分化期間至雌性發育階段其表現逐漸上升,至一年齡有一表現高峰,隨後下降,至兩~三年齡皆呈現生理低表現,於3+7 -age再度有一cyp19a1a表現高峰。由IHC染色結果,P450arom於性別分化後的雌性germ cell (OG, CA, VO) 外之濾泡層表現,尤其在ZR層明顯增厚階段卵細胞,P450arom只在濾泡層之granulose cells表現。無論任何階段雌性卵巢P450arom皆不會在初級卵母細胞 (PO) 表現。人工性轉變後P450arom微弱表現於SSC/VSCs階段生殖腺之精原細胞 (spermatogonium, SG) 外體細胞,與內含精母細胞 (spermatocyte, SC)、精細胞 (spermatid, SD) 包囊之間質細胞表現,以及退化卵細胞 (atretic oocyte) 外圍濾泡層細胞。
點帶石斑dmrt1專一表現於7月齡點帶石斑生殖腺,dmrt1於生殖腺性別分化過程逐漸上升,並高度表現於性轉變後精巢 (IHC結果Dmrt1強烈表現於SG細胞質);由in situ與IHC結果顯示dmrt1 (Dmrt1) 於性別分化期間與早期雌性發育卵巢之germ cells細胞質表現,且同時表現於卵巢與精巢之germ cells,因此認為點帶石斑的Dmrt1不止參與精巢的發育,亦對於早期雌性的分化 (卵細胞的生成、卵巢空腔的維持) 相當重要。由cyp19a1a與dmrt1基因表現與IHC結果,推測secondary male的發生可能在2+3 齡之後,2+3 齡亦是區別primary male與secondary male關鍵點。
性別分化及內分泌相關等基因,於性別分化期間 (4-5個月齡) 皆會有上升之表現,而在MT或AI投餵處理後,抑制生殖腺雌性生殖配子 (OG) 生成與發育、卵巢空腔 (OC) 萎縮或形成VSCs精巢結構,造成生殖腺dax1、foxL2、cyp19a1a、vasa、與pcna等基因於性別分化期間表現顯著下降,sf1與dmrt1基因則表現顯著上升,其結果亦反應在P450arom、Dmrt1、Pcna與Vasa免疫組織染色上,故認為dax1、foxL2、cyp19a1a等基因有助於點帶石斑早期雌性分化與發育,sf1與dmrt1對點帶石斑雄性性別分化上扮演重要角色。

The sex of Orange-Spotted grouper gonad (Epinephelus coioides) firstly differentiated into female, each lobe of the paired gonads began to form an ovarian cavity at 4 mo of age. The formation of the ovarian cavity is an early characteristic of female differentiation for orange-spotted grouper. The newly budded ovarian lamella around the inside of the ovarian cavity in the gonad mainly contained oogonia and a few primary oocytes in the fish at 6.5-7 mo of age. Cortical alveoli (CA) appeared untill 1yr7mo age,and vitellogenic oocytes were present within the mature ovarian lamellae.Treament with aromatase inhibiter (AI) or methyltestosterone (MT) by feed and implantation in sex differentiation period (3-5mo)、1-2yr and 4yr adult fish inducesd sex change. To investigate gene expression of cyp19a1a、dmrt1 and sex-differentiation related genes ex. sf1、dax1、foxL2、11β, and localization of P450arom、Dmrt1、Pcna、Vasa during sex differentiation and sex change by immunohistochemistry (IHC).
The protogynous orange-spotted grouper E. coioides is a diandric type of hermaphrodite. Most secondary males occur through a sex change in adult female fish and few primary males develop directly from juveniles. primary males and secondary males have varying degree sex change testis tissue:a single spermatogenic cyst (SSC)、various stages of spermatogenic cyst (VSCs) and mature stage of cysts (AMC). Primary males (sex change naturally) with single spermatogenic cyst and various stages of spermatogenic cyst firstly occur in 1.5 yr.
The expressions of cyp19a1a is single-mindedly in 7 mo orange-spotted grouper gonad. The gene expression of cyp19a1a expressed increasingly from sex differentiation to female adult fish. There are two gene expression peaks of cyp19a1a in 1 and 3+7 -age. From IHC results, P450arom appeared in follicular layers of oogonia, cortical alveolar oocytes, and vitellogenic oocytes but not in primary oocytes. It was interesting to observe that testicular tissues in the sex-changing male grouper (such as cysts,spermatids, and spermatogonia) showed positive aromatase staining.
The expressions of dmrt1 is only in 7 mo orange-spotted grouper gonad. The gene expression is gradually expressed increasingly during sex differentiation and highly expressed in testis after sex change (From IHC results, Dmrt1 express strongly in cytoplasm of SG);From IHC and in situ results, Dmrt1 (dmrt1) expresses in cytoplasm of germ cells during sex differentiation or early female gonad development stage, and also expresses in the germ cells of ovary or testis. So dmrt1 is vary important not only involved in testis growth but also in early female differentiation (including the production of oocytes and maintain of ovarian cavity). Based on the gene expression of cyp19a1a and dmrt1 and IHC results, primary male first appears at 1+5 years. The expression peak of dmrt1 indicates that 2+3 years is critical time point to distinguish primary and secondary males.
Sex-differentiation and endocrine related genes expressed increasingly during sex differentiation period, but treatment with MT or AI, it inhibit the female gonad germ cell production and growth、the atrophy ovarian cavity or formation of various stages of spermatogenic cyst (VSCs).
MT induces musculinization which causes the decreased expression of dax1, foxL2, cyp19a1a, vasa, pcna and the increased expression of sf1 and dmrt1. Therefore, dax1, foxL2, cyp19a1a might be related with early sex differentiation and development. sf1 and dmrt1 might play an important role in male sex differentiation of E. coioides.

目 錄
頁次

謝辭 ...................................................i
中文摘要 ...............................................ii
英文摘要 ...............................................v
目錄................................................. viii
圖表目錄................................................x
緒論...................................................1
第一章、幼魚、成魚至性轉變雄魚之生殖腺組織結構發育變化
壹、前言................................................9
貳、材料與方法..........................................11
參、結果...............................................16
肆、討論...............................................23

第二章、環化酵素抑制物處理對生殖腺性別分化與性轉變之基因表現及組織細胞發育特性探討 (aromatase、DMRT1)
壹、前言 ..............................................40
貳、材料與方法..........................................45
參、結果...............................................63
肆、討論...............................................74


第三章、甲基睪固酮處理於生殖腺性別分化期間與性別分化相關基因探討
壹、前言...............................................111
貳、材料與方法..........................................114
參、結果 ..............................................128
肆、討論...............................................136
總結...................................................161
參考文獻...............................................163

參考文獻

Afonso, L. O. B., Iwama, G. K., Smith, J., Donaldson, E. M., 2000. Effects of the aromatase inhibitor fadrozole on reproductive steroids and spermiation in male coho salmon (Oncorhynchus kisutch) during sexual maturation. Aquaculture 188, 175-187.
Ahmed, S., 1998. Review of the molecular modelling studies of the cytochrome P-450 estrogen synthetase enzyme, aromatase. Drug Des. Discov. 15, 239-252.
Alam, M. A., Bhandari, R. K., Kobayashi, Y., Soyano, K., Nakamura, M., 2006. Changes in androgen-producing cell size and circulating 11-ketotestosterone levels during female-to-male sex change in honeycomb grouper (Epinephelus merra). Mol. Reprod. Dev. 73, 206-214.
Alam, M. A., Kobayashi, Y., Horiguchi, R., Hirai, T., Nakamura, M., 2008. Molecular cloning and quantitative expression of sexually dimorphic markers Dmrt1 and Foxl2 during female-to-male sex change in Epinephelus merra. Gen. Comp. Endocrinol. 157, 75-85.
Amer, M. A., Miura, T., Miura, C., Yamauchi, K., 2001. Involvement of sex steroid hormones in the early stages of spermatogenesis in Japanese huchen (Hucho perryi). Biol. Reprod. 65, 1057-1066.
Amores, A., Force, A., Yan, Y. L., Joly, L., Amemiya, C., Fritz, A., Ho, R. K., Langeland, J., Prince, V., Wang, Y. L., 1998. Zebrafish hox clusters and vertebrate genome evolution. Science 282, 1711-1714.
An, K. W., Nelson, E. R., Jo, P. G., Habibi, H. R., Shin, H. S., Choi, C. Y., 2008. Characterization of estrogen receptor β2 and expression of the estrogen receptor subtypes α, β1, and β2 in the protandrous black porgy (Acanthopagrus schlegeli) during the sex change process. Comp. Biochem. Physiol. 150, 284-291.
Andreassen, T. K., Skjoedt, K., Anglade, I., Kah, O., Korsgaard, B., 2003. Molecular cloning, characterisation, and tissue distribution of oestrogen receptor alpha in eelpout (Zoarces viviparus). Gen. Comp. Endocrinol. 132, 356-368.
Ankley, G. T., Kahl, M. D., Jensen, K. M., Hornung, M. W., Korte, J. J., Makynen, E. A., Leino, R. L., 2002. Evaluation of the aromatase inhibitor fadrozole in a shortterm reproduction assay with the fathead minnow (Pimephales promelas). Toxicol. Sci. 67, 121-130.
Asoh, K., Kasuya, M., 2002. Gonadal development and mode of sexuality in a coral-reef dameselfish, Dascyllus trimaculatus. J. Zool. Lond. 256: 301-309.
Bain, D. L., Heneghan, A. F., Connaghan-Jones, K. D., Miura, M. T., 2007. Nuclear receptor structure: implications for function. Annu. Rev. Physiol. 69, 201-220.
Bakke, M., Zhao, L. P., Parker, K. L., 2001. Approaches to define the
Bardoni, B., Zanaria, E., Guioli, S., Floridia, G., Worley, K. C., Tonini, G., Ferrante, E., Chiumello, G., McCabe, E. R., Fraccaro, M., 1994. A dosage sensitive locus at chromosome Xp21 is involved in male to female sex reversal. Nat. Genet. 7, 497-501.
Bancroft, J. D., Cook, H. C., 1994. Clinical Cytology. Manual of Histological Techniques and their Diagnostic Application. Edinburgh: Churchill Livingstone, pp. 323-342.
Bardoni, B., Zanaria, E., Guioli, S., Floridia, G., Worley, K., Tonini, G., Ferrante, E., Chiumellw, F., McCabe, E., Fraccaro, M., Zuffardi, O., Camerino, G., 1994. A dosage sensitive locus at chromosome Xp21 is involved in male to female sex reversal. Nat. Genet. 7, 497-501.
Barney, M. L., Patil, J. G., Gunasekera, R. M., Carter, C. G., 2008. Distinct cytochrome P450 aromatase isoforms in the common carp (Cyprinus carpio): sexual dimorphism and onset of ontogenic expression. Gen. Comp. Endocrinol. 156, 499-508.
Baroiller, J. F., D’Cotta, H., Saillant, E., 2009. Environmental effects on fish sex determination and differentiation. Sex Dev. 3, 118-135.
Baroiller, J. F., Guiguen, Y., Fostier, A., 1999. Endocrine and environmental aspects of sex differentiation in fish. Cell Mol. Life Sci. 55, 910-931.
Baron, D., Houlgatte, R., Fostier, A., Guiguen, Y., 2005. Large-scale temporal gene expression profiling during gonadal differentiation and early gametogenesis in rainbow trout. Biol. Reprod. 73, 959-966.
Baron, D., Montfort, J., Houlgatte, R., Fostier, A., Guiguen, Y., 2007. Androgeninduced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles. BMC. Genomics 8, 357-365.
Besseau, L., Bruslé-Sicard, S., 1995. Plasticity of gonad development in hermaphroditic sparids: ovotestis ontogeny in a protandric species, Lithognathus mormyrus. Environ. Biol. Fish. 43: 255-297.
Bhandari, R. K., Alam, M. A., Soyano, K., Nakamura, M., 2006. Induction of female-to male sex change in the honeycomb grouper, Epinephelus merra by 11-ketotestosterone treatments. Zool. Sci. 23, 65-69.
Bhandari, R. K., Komuro, H., Higa, M., Nakamura, S., Nakamura, M., 2003. Gonadal restructuring and correlative sex steroid profiles during protogynous sex change in the honeycomb grouper (Epinephelus merra). Zool. Sci. 20, 1399-1404.
Bhandari, R. K., Higa, M., Nakamura, S., Nakamura, M., 2004. Aromatase inhibitor induces complete sex change in the protogynous honeycomb grouper (Epinephelus merra). Mol. Reprod. Dev. 67, 303-307.
Blazquez, M., Gonzalez, A., Papadaki, M., Mylonas, C., Piferrer, F., 2008. Sexrelated changes in estrogen receptors and aromatase gene expression and enzymatic activity during early development and sex differentiation in the European sea bass (Dicentrarchus labrax). Gen. Comp. Endocrinol. 158, 95-101.
Blazquez, M., Gonzalez, A., Papadaki, M., Mylonas, C., Piferrer, F., 2008. Sex-related changes in estrogen receptors and aromatase gene expression and enzymatic activity during early development and sex differentiation in the European sea bass (Dicentrarchus labrax). Gen. Comp. Endocrinol. 158, 95-101.
Blazquez, M., Piferrer, F., 2004. Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax). Mol. Cell. Endocrinol. 219, 83-94.
Blazquez, M., Piferrer, F., 2005. Sea bass (Dicentrarchus labrax) androgen receptor: cDNA cloning, tissue-specific expression, and mRNA levels during early development and sex differentiation. Mol. Cell. Endocrinol. 237, 37-48.
Bouma, J., Nagler, J. J., 2001. Estrogen receptor-a protein localization in the testis of the rainbow trout (Oncorhynchus mykiss) during different stages of the reproductive cycle. Biol. Reprod. 65, 60-65.
Breitwieser, W., Markussen, F., Horstmann, H., Ephrussi, A., 1996. Oskar protein interaction with vasa represents an essential step in polar granule assembly. Genes Dev. 10, 2179-2188.
Breton, B., Sambroni, E., 1996. Steroid activation of the brain-pituitary complex gonadotropic function in the triploid rainbow trout Oncorhynchus mykiss. Gen. Comp. Endocrinol. 101, 155-164.
Brunner, B., Hornung, U., Shan, Z., Nanda, I., Kondo, M., Zend-Ajusch, E., Haaf, T., Ropers, H. H., Shima, A., Schmid, M., Kalscheuer, V. M., Schartl, M., 2001. Genomic organization and expression of the doublesex-related gene cluster in vertebrates and detection of putative regulatory regions for DMRT1. Genomics 77, 8-17.
Byers, M., Kuiper, G., Gustafsson, J. A., Park-Sarge, O. K., 1997. Estrogen receptor-beta mRNA expression in rat ovary: down- regulation by gonadotropins. Mol. Endocrinol. 11, 172-182.
Cardinali, M., Gioacchini, G., Candiani, S., Pestarino, M., Yoshizaki, G., Carnevali, O., 2004. Hormonal regulation of vasa-like messenger RNA expression in the ovary of the marine teleost Sparus aurata. Biol. Reprod. 70, 737-743.
Carlsson, P., Margit, M., 2002. Forkhead Transcription factors: key players in development and metabolism. Developmental Biology 250, 1-23.
Chang, C. F., Lee, M. F., Chen, G. L., 1994. Estrodiol-17β associated with the sexreversal in protandrous black porgy, Acanthopagrus schlegeli. J. Exp. Zool. 268, 53-58.
Chang, C. F., Yueh, W. S., 1990. Annual cycle of gonad histology and steroid profiles in the juvenile males and adult females of the protandrous black porgy, Acanthopagrus schlegeli. Aquaculture 91, 179-196.
Chang, C. F., Lin, B. Y., Lau, E. L., Lee, M. F., Yueh, W. S., Lee, Y. H., Chang, C. N., Huang, J. D., Tacon, P., Lee, F. Y., Du, J. L., Sun, L. T., 1997. The endocrine mechanism of sex reversal in the protandrous black porgy, Acanthopagrus schlegeli: a review. Chin. J. Physiol. 40, 197-205.
Chang, X. T., Kobayashi, T., Senthilkumaran, B., Kobayashi-Kajura, H., Sudhakumari, C. C., Nagahama, Y., 2005. Two types of aromatase with different encoding genes, tissue distribution and developmental expression in Nile tilapia, Oreochromis niloticus. Gen. Comp. Endocrinol. 141, 101-115.
Cheng, A. C., Chen, C. Y., Liou, C. H., Chang, C. F., 2006. The effects of dietary protein and lipid in the blood parameters and superoxide anion prodution in grouper, Epinephelus coioides. Zool. Stud. 45, 492-502.
Choi, C. Y., Habibi, H. R., 2003. Molecular cloning of estrogen receptor a and expression pattern of estrogen receptor subtypes in male and female goldfish, Mol. Cell. Endocrinol. 204, 169-177.
Choi, C. Y., Habibi, H. R., 2005. Molecular cloning and tissue distribution of SF-1-related orphan receptors during sexual maturation in female goldfish. Biotechnol. Lett. 27, 1283-1290.
Cocquet, J., Pailhoux, E., Jaubert, F., Servel, N., Xia, X., Pannetier, M., De Baere, E., Messiaen, L., Cotinot, C., Fellous, M., Veitia, R. A., 2002. Evolution and expression of FOXL2. J. Med. Genet. 39, 916-921.
Corley, D. R., Li, X., Lei, Z. M., Rao, C. V., 2000. Potential regulation of GnRH gene by a steroidogenic factor-1-like protein. Mol. Hum. Reprod. 6, 671-676.
Crisponi, L., Deiana, M., Loi, A., Chiappe, F., Uda, M., Amati, P., Bisceglia, L., Zelante, L., Nagaraja., R., Porcu, S., Ristaldi, M. S., Marzella, R., Rocchi, M., Nicolino, M., Lienhardt-Roussie, A., Nivelon, A., Verloes, D., Schlessinger, P., Gasparini, D., Bonneau, A., Cao, A., Pilia, G., 2001. The putative forkhead transcription factor FOXL2 is mutated in blepharophimosis/ptosis/epicanthus inversus syndrome, Nat. Genet. 27, 159-166.
Dacheux, F., Martinat, N., 1983. Immunocytochemical localization of LH, FSH and TSH in the fetal porcine pituitary. Cell Tissue Res. 228, 277-295.
Danielian, P. S., White, R., Lees, J. A., Parker, M. G., 1992. Identification of a conserved region required for hormone dependent transcriptional activation by steroid hormone receptors, EMBO J. 11, 1025-1033.
Dellovade, T. L., Young, M., Ross, E. P., Henderson, R., Caron, K., Parker, K., Tobet, S. A., 2000. Disruption of the gene encoding SF-1 alters the distribution of hypothalamic neuronal phenotypes. J. Comp. Neurol. 423, 579-589.
Deloffre, Laurence, A. M., Martins, Rute, S. T., Mylonas, Constantinos, C., Canario, Adelino, V. M., 2009. Alternative transcripts of DMRT1 in the European sea bass: Expression during gonadal differentiation. Aquaculture 293, 89-99.
Devlin, R. H., Nagahama, Y., 2002. Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture 208, 191-364.
Dubois, P. M., Begeot, M., Dubois, M. P., Herbert, D. C., 1978. Immunocytological localization of LH, FSH, TSH and their subunits in the pituitary of normal and anencephalic human fetuses. Cell Tissue Res. 191, 249-265.
Dufau, M. L., 1998. The luteinizing hormone receptor. Ann. Rev. Physiol. 60, 461-496.
Duval, D. L., Nelson, S. E., Clay, C. M., 1997. A binding site for steroidogenic factor-1 is part of a complex enhancer that mediates expression of the murine gonadotropin-releasing hormone receptor gene. Biol. Reprod. 56, 160-168.
Ebling, F., Brooks, A. N., Cronin, A. S., Ford, H., Kerr, J. B., 2000. Estrogenic induction of spermatogenesis in the hypogonadal mouse. Endocrinology 141, 2861-2869.
Erdman, S. E., Burtis, K. C., 1993. The Drosophila doublesex proteins share a novel zinc finger related DNA-binding domain. Embo J. 12, 527-535.
Fenske, M., Segner, H., 2004. Aromatase modulation alters gonadal differentiation in developing zebrafish, Danio rerio. Aquat. Toxicol. 67, 105-126.
Fernandino, J. I., Guilgur, L. G., Somoza, G. M., 2006. Dmrt1 expression analysis during spermatogenesis in pejerrey, Odontesthes bonariensis. Fish Physiol. Biochem. 32, 231-240.
Filby, A. L., Tyler, C. R., 2005. Molecular characterization of estrogen receptors 1, 2α, and 2β and their tissue and ontogenic expression profiles in fathead minnow (Pimephales promelas). Biol. Reprod. 73, 648-662.
Fleming, A., Wibbels, T., Skipper, J. K., Crews, D., 1999. Developmental expression of steroidogenic factor 1 in a turtle with temperature- dependent sex determination. Gen. Comp. Endocrinol. 116, 336-346.
Fu, K. Y., Chen, C. Y., Lin, C. T., Chang, W. M., 2008. Molecular cloning and tissue distribution of three estrogen receptors from the cyprinid fish Varicorhinus barbatulus. J. Comp. Physiol. 178, 189-197.
Garcia, A. A., Villaplana, M., Garcia Hernandez, M. P., Chaves, P. E., Agulleiro, B., 2003. FSH, LH, and TSH expressing cells during development of Sparus aurata L. (Teleostei). An immunocytochemical study. Gen. Comp. Endocrinol. 134, 72-79.
Goodfellow, P., Lovell-Badge, R., 1993. SRY and sex determination in mammals. Annu. Rev. Genet. 27, 71-92.
Govoroun, M. S., Pannetier, M., Pailhoux, E., Cocquet, J., Brillard, J. P., Couty, I., Batellier, F., Cotinot, C., 2004. Isolation of chicken homolog of the FOXL2 gene and comparison of its expression patterns with those of aromatase during ovarian development. Dev. Dyn. 231, 859-870.
Grandcourt, E. M., Al Abdessalaam, T. Z., Francis, F., Al Shamsi, A. T., Hartmann, S. A., 2009. Reproductive biology and implications for management of the orange-spotted grouper Epinephelus coioides in the southern Arabian Gulf. J. Fish Biol. 74: 820-841.
Greytak, S. R., Callard, G. V., 2007. Cloning of three estrogen receptors (ER) from killifish (Fundulus heteroclitus): differences in populations from polluted and reference environments. Gen. Comp. Endocrinol. 150, 174-188.
Gruidl, M., Smith, P., Kuznicki, K., McCrone, J., Kirchner, J., Roussell, D., Strome, S., Bennett, K., 1996. Multiple potential germ-line helicases are components of the germ-line-specific P granules of Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 93, 13837-13842.
Guan, G., Kobayashi, T., Nagahama, Y., 2000. Sexually dimorphic expression of two types of DM (Doublesex/Mab-3)-domain genes in a teleost fish, the tilapia, Oreochromis niloticus. Biochem. Biophys. Res. Commun. 272, 662-666.
Guo, Y., Cheng, H., Huang, X., Gao, S., Yu, H., Zhou, R., 2005. Gene structure, multiple alternative splicing, and expression in gonads of zebrafish dmrt1. Biochem. Biophys. Res. Commun. 330, 950-957.
Guzman, J. M., Bayarri, M. J., Ramos, J., Zohar, Y., Sarasquete, C., Mananos, E. L., 2009. Follicle stimulating hormone (FSH) and luteinizing hormone (LH) gene expression during larval development in Senegalese sole (Solea senegalensis). Comp. Biochem. Physiol. 154, 37-43.
Halm, S., Martinez-Rodriguez, G., Rodriguez, L., Prat, F., Mylonas, C. C., Carrillo, M., Zanuy, S., 2004. Cloning, characterisation, and expression of three oestrogen receptors (ERα, ERβ1 and ERβ2) in the European sea bass, Dicentrarchus labrax. Mol. Cell. Endocrinol. 223, 63-75.
Halvorson, L. M., Kaiser, U. B., Chin, W. W., 1996. Stimulation of luteinizing hormone beta gene promoter activity by the orphan nuclear receptor, steroidogenic factor-1. J. Biol. Chem. 271, 6645-6650.
Hatano, O., Takayama, K., Imai, T., Waterman, M. R., Takakusu, A., Omura, T., Morohashi, K., 1994. Sex-dependent expression of a transcription factor, Ad4BP, regulating steroidogenic P-450 genes in the gonads during prenatal and postnatal rat development. Dev. Biol. 120, 2787-2797.
Hattori, R. S., Gould, R. J., Fujioka, T., Saito, T., Kurita, J., Strüssmann, C. A., Yokota, M., Watanabe, S., 2007. Temperature-dependent sex determination in Hd-rR medaka Oryzias latipes: gender sensitivity, thermal threshold, critical period, and dmrt1 expression profile. Sex. Dev. 1, 138-146.
Hawkins, M. B., Thornton, J. W., Crews, D., Skipper, J. K., Dotte, A., Thomas, P., 2000. Identification of a third distinct estrogen receptor and reclassification of estrogen receptors in teleosts. Proc. Natl. Acad. Sci. USA 97, 10751-10756.
Hay, B., Jan, L., Jan, Y., 1988. A protein component of drosophila polar granules is encoded by vasa and has extensive sequence similarity to ATP-dependent helicases. Cell 55, 577-587.
Hayes, T. B., 1998. Sex determination and primary sex differentiation in amphibians: genetic and developmental mechanisms. J. Exp. Zool. 281, 373-399.
He, C. L., Du, J. L., Wu, G. C., Lee, Y. H., Sun, L. T., Chang, C. F., 2003. Differential Dmrt1 transcripts in gonads of the protandrous black porgy, Acanthopagrus schlegeli. Cytogenet. Genome Res. 101, 309-313.
Herpin, A., Rohr, S., Riedel, D., Kluever, N., Raz, E., Schartl, M., 2007. Specification of primordial germ cells in medaka (Oryzias latipes). BMC Dev. Biol. 7, 1-10.
Hess, R. A., 2003. Estrogen in the adult male reproductive tract: a review, Reprod. Biol. Endocrinol. 1, 52-65.
Hofsten, J., Per-Erik, O., 2005. Zebrafish sex determination and differentiation: Involvement of FTZ-F1 genes. Reprod. Biol. Endocrinol. 3, 1-11.
Hu, M. C., Hsu, N. C., Pai, C. I., Wang, C. K., Chung, B., 2001. Functions of the upstream and proximal steroidogenic factor 1 (SF-1)-binding sites in the CYP11A1 promoter in basal transcription and hormonal response. Mol. Endocrinol. 15, 812-818.
Huang, J. D., Lee, M. F., Chang, C. F., 2002. The morphology of gonadal tissue and male germ cell in the protandrous black porgy, Acanthopagrus schlegeli. Zool. Stud. 41, 216-227.
Hunter, G. A., Donaldson, E. M., 1983. Hormonal sex control and its application to fish culture. Fish Physiol. 9, 223-303.
Ijiri, S., Kaneko, H., Kobayashi, T., Wang, D. S., Sakai, F., Paul-Prasanth, B., Nakamura, M., Nagahama, Y., 2008. Sexual dimorphic expression of genes in gonads during early differentiation of a teleost fish, the Nile tilapia Oreochromis niloticus. Biol. Reprod. 78, 333-341.
Ikeda, Y., Lala, D. S., Luo, X., Kim, E., Moisan, M., Parker, K., 1993. Characterization of the mouse FTZ-F1 gene, which encodes a key regulator of steroid hydroxylase gene expression. Mol. Endocrinol. 7, 852-860.
Ikeda, Y., Luo, X., Abbud, R., Nilson, J. H., Parker, K. L., 1995. The nuclear receptor steroidogenic factor 1 is essential for the formation of the ventromedial hypothalamic nucleus. Mol. Endocrinol. 9, 478-486.
Ingraham, H. A., Lala, D. S., Ikeda, Y., Luo, X., Shen, W. H., Nachtigal, M. W., Abbud, R., Nilson, J. H., Parker, K. L., 1994. The nuclear receptor steroidogenic factor 1 acts at multiple levels of the reproductive axis. Genes Dev. 8, 2302-2312.
Joel, P. B., Smith, J., Sturgill, T. W., Fisher, T. L., Blenis, J., Lannigan, D. A., 1998. pp90rsk1 regulates estrogen receptor-mediated transcription through phosphorylation of Ser-167, Mol. Cell. Biol. 18, 1978-1984.
Johnson, A. L., Bridgham, J. T., Wagner, B., 1996. Characterization of a chicken luteinizing hormone receptor (cHL-R) complementary deoxyribonucleic acid, and expression of cHL-R messenger ribonucleic acid in the ovary. Biol. Reprod. 55, 304-309.
Jorgensen, A., Morthorst, J. E., Andersen, O., Rasmussen, L. J., Bjerregaard, P., 2008. Expression profiles for six zebrafish genes during gonadal sex differentiation. Reprod. Biol. Endocrinol. 6, 6-25.
Kawasaki, I., Shim, Y. H., Kirchner, J., Kaminker, J., Wood, W. B., Strome, S., 1998. PGL-1, a predicted RNA-binding component of germ granules, is essential for fertility in C. elegans. Cell 94, 635-645.
Kettlewell, J., Raymond, C., Zarkower, D., 2000. Temperature-dependent expression of turtle Dmrt1 prior to sexual differentiation. Genesis 26, 174-178.
Kim, S., Kettlewell, J. R., Anderson, R. C., Bardwell, V. J., Zarkower, D., 2003. Sexually dimorphic expression of multiple doublesex-related genes in the embryonic mouse gonad. Gene Expr. Patterns 3, 77-82.
Kim, Y., Capel, B., 2006. Balancing the bipotential gonad between alternative organ fates: a new perspective on an old problem. Dev. Dyn. 235, 2292-2300.
Kitahashi, T., Ogawa, S., Soga, T., Sakuma, Y., Parhar, I., 2007. Sexual maturation modulates expression of nuclear receptor types in laser-captured single cells of the cichlid (Oreochromis niloticus) pituitary. Endocrinology 148, 5822-5830.
Kitano, T., Takamune, K., Nagahama, Y., Abe, S. I., 2000. Aromatase inhibitor and 17α-methyltestosterone cause sex-reversal from genetical females to phenotypic males and suppression of P450 aromatase gene expression in Japanese flounder, Paralichthys olivaceus. Mol. Reprod. Develop. 56, 1-5.
Kitano, T., Yoshinaga, N., Shiraishi, E., Koyanagi, T., Abe, S., 2007. Tamoxifen induces masculinization of genetic females and regulates P450 aromatase and mullerian inhibiting substance mRNA expression in Japanese flounder (Paralichthys olivaceus). Mol. Reprod. Dev. 74, 1171-1177.
Kobayashi, T., Kajiura-Kobayashi, H., Guan, G., Nagahama, Y., 2008. Sexual dimorphic expression of dmrt1 and Sox9a during gonadal differentiation and hormoneinduced sex reversal in the teleost fish Nile tilapia (Oreochromis niloticus). Dev. Dyn. 237, 297-306.
Kobayashi, T., Kajiura-Kobayashi, H., Nagahama, Y., 2000. Differential expression of vasa homologue gene in the germ cells during oogenesis and spermatogenesis in a teleost fish, tilapia, Oreochromis niloticus. Mech. Dev. 99, 139-142.
Kobayashi, T., Kajiura-Kobayashi, H., Nagahama, Y., 2002. Two isoforms of vasa homologs in a teleost fish: their differential expression during germ cell differentiation. Mech. Dev. 111, 167-171.
Kobayashi, T., Matsuda, M., Kajiura-Kobayashi, H., Suzuki, A., Saito, N., Nakamoto, M., Shibata, N., Nagahama, Y., 2004. Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes. Dev. Dyn. 231, 518-526.
Kobayashi, T., Morrey, C. E., Shibata, N., Asakawa, S., Shimizu, N., Hori, H., Hamaguchi, S., Sakaizumi, M., 2002. DMY is a Y-specific DM-domain gene required for male development in the medaka fish. Nature 417, 559-563.
Kohn, L. D., Shimura, H., Shimura, Y., Hidaka, A., Giuliani, C., Napolitano, G., Ohmori, M., Laglia, G., Saji, M., 1995. The thyrotropin receptor. Vit. Horm. 50, 287-384.
Kojetin, D. J., Burris, T. P., Jensen, E. V., Khan, S. A., 2008. Implications of the binding of tamoxifen to the coactivator recognition site of the estrogen receptor. Endocr. Relat. Cancer 15, 851-870.
Kondo, M., Nanda, I., Hornung, U., Schmid, M., Schartl, M., 2004. Evolutionary origin of the medaka Y chromosome. Curr. Biol. 14, 1664-1669.
Krege, J. H., Hodgin, J. B., Couse, J. F., Enmark, E., Warner, M., Mahler, J. F., Sar, M., Korach, K. S., Gustafsson, J. A., Smithies, O., 1998. Generation and reproductive phenotypes of mice lacking estrogen receptor beta, Proc. Natl. Acad. Sci. USA 95, 15677-15682.
Krøvel, A., Olsen, L., 2004. Sexual dimorphic expression pattern of a splice variant of zebrafish vasa during gonadal development. Dev. Biol. 271, 190-197.
Kumar, R. S., Ijiri, S., Trant, J. M., 2001. Molecular biology of the channel catfish gonadotropin receptors: Complementary DNA cloning, functional expression, and seasonal gene expression of the follicle- stimulating hormone receptor. Biol. Reprod. 65, 710-717.
Kurokawa, H., Saito, D., Nakamura, S., Katoh-Fukui, Y., Ohta, K., Baba, T., Morohashi, K., Tanaka, M., 2007. Germ cells are essential for sexual dimorphism in the medaka gonad. Proc. Natl. Acad. Sci. USA. 104, 16958-16963.
Kusakabe, M., Kobayashi, T., Todo, T., Mark, L. P., Nagahama, Y., Young, G., 2002. Molecular cloning and expression during spermatogenesis of a cDNA encoding testicular 11beta-hydroxylase (P45011beta) in rainbow trout (Oncorhynchus mykiss). Mol. Reprod. Dev. 62, 456-469.
Kwok, H. F., So, W. K., Wang, Y., Ge, W., 2005. Zebrafish gonadotropins and their receptors: Cloning and characterization of zebrafish follicle- stimulating hormone and luteinizing hormone receptors-evidence for their distinct functions in follicle development. Biol. Reprod. 72, 1370-1381.
Kwon, J. Y., Haghpanah, V., Kogson-Hurtado, L. M., McAndrew, J., Penman, D. J., 2000. Masculinization of genetic female nile tilapia (Oreochromis niloticus) by dietary administration of an aromatase inhibitor during sexual differentiation. J. Exp. Zool. 287, 46-53.
Kwon, J. Y., McAndrew, B. J., Penman, D. J., 2001. Cloning of brain aromatase gene and expression of brain and ovarian aromatase genes during sexual differentiation in genetic male and female nile tilapia, Oreochromis niloticus. Mol. Reprod. Dev. 59, 359-370.
Lance, V.A., Bogart, M.H., 1991. Tamoxifen “sex reverses” alligator embryos at maleproducing temperature,but is an antiestrogen in female hatchlings. Cell. Mol. Life Sci. 47, 263-266.
Lassiter, C. S., Kelley, B., Linney, E., 2002. Genomic structure and embryonic expression of estrogen receptor beta a (ER beta a) in zebrafish (Danio rerio). Gene 299, 141-151.
Leal, M.C., Cardoso, E.R., Nobrega, R.H., Batlouni, S.R., Bogerd, J., Franca, L.R., Schulz, R.W., 2009. Histological and stereological evaluation of zebrafish (Danio rerio) spermatogenesis with an emphasis on spermatogonial generations. Biol. Reprod. 81, 177-187.
Lee, M. F., Huang, J. D., Chang, C. F., 2008. The development of ovarian tissue and female germ cells in the protandrous black porgy fish, Acanthopagrus schlegeli. Zool. Stud. 47: 302-316.
Lee, M. F., Huang, J. D., Chang, C. F., 2011. Development of the genital duct system in the protandrous black porgy, Acanthopagrus schlegeli. Anat. Rec. 294: 494-545.
Lee, Y. H., Lee, F. Y., Yueh, W. S., Tacon, P., Du, J. L., Chang, C. N., Jeng, S. R., Tanaka, H., Chang, C. F., 2000. Profiles of gonadal development, sex steroids, aromatase activity, and gonadotropin II in the controlled sex change of protandrous black porgy, Acanthopagrus schlegeli Bleeker. Gen. Comp. Endocrinol. 119, 111-120.
Lee, Y. H., Wu, G. C., Chang, C. F., 2004. Estradiol-17β induced a reversible sex change in the fingerlings of protandrous black porgy, Acanthopagrus schlegeli Bleeker: the possible roles of luteinizing hormone in sex change. Biol. Reprod. 71, 1270-1278.
Lee, Y. H., Yueh, W. S., Du, J. L., Sun, L. T., Chang, C. F., 2002. Aromatase inhibitors block natural sex change and induce male function in the protandrous black porgy, Acanthopagrus schlegeli Bleeker: possible mechanism of natural sex change. Biol. Reprod. 66, 1749-1754.
Leers-Sucheta, S., Morohashi, K., Mason, J. I., Melner, M. H., 1997. Synergistic activation of the human type II 3beta-hydroxysteroid dehydrogenase/delta5-delta4 isomerase promoter by the transcription factor steroidogenic factor-1/adrenal 4-binding protein and phorbol ester. J. Biol. Chem. 272, 7960-7967.
Lei, N., Heckert, L. L., 2004. Gata4 regulates testis expression of Dmrt1. Mol. Cell. Biol. 24, 377-388.
Lephart, E. D., 1996. A review of brain aromatase cytochrome P450. Brain Research Reviews, Volume 22, Issue 1, 1-26.
Li, G. L., Liu, X. C., Lin, H. R., 2005. Aromatase inhibitor letrozole induces sex inversion in the protogynous red spotted grouper, Epinephelus akaara. Acta. Physiologica. Sinica. 57, 473-479.
Li, G. L., Liu, X. C., Lin, H. R., 2006. Effects of 17α-methyltestosterone on sex reversal in red-spotted grouper, Epinephelus akaara. J. Fish China 30, 145-150.
Liarte, S., Chaves-Pozo, E., García-Alcazar, A., Mulero, V., Meseguer, J., García-Ayala, A., 2007. Testicular involution prior to sex change in gilthead seabream is characterized by a decrease in DMRT1 gene expression and by massive leukocyte infiltration. Reprod. Biol. Endocrinol. 5, 1-15.
Liu, J. F., Guiguen, Y., Liu, S. J., 2009. Aromatase (P450arom) and 11β-hydroxylase (P45011β) genes are differentially expressed during the sex change process of the protogynous rice field eel, Monopterus albus. Fish Physiol. Biochem. 35, 511-518.
Liu, M., de Mitcheson, Y. S., 2009. Gonad development during sexual differentiation in hatchery-produced orange-spotted grouper (Epinephelus coioides) and humpback grouper (Cromileptes altivelis) (Pisces: Serranidae, Epinephelinae). Aquaculture 287, 191-202.
Liu, Z., Wu, F., Jiao, B., Zhang, X., Hu, C., Huang, B., Zhou, L., Huang, X., Wang, Z., Zhang, Y., Nagahama, Y., Cheng, C. H. K., Wang, D., 2007. Molecular cloning of doublesex and mab-3-related transcription factor 1, forkhead transcription factor gene 2, and two types of cytochrome P450 aromatase in Southern catfish and their possible roles in sex differentiation. J. Endocrinol. 194, 223-241.
Loffler, K. A., Zarkower, D., Koopman, P., 2003. Etiology of ovarian failure in blepharophimosis ptosis epicanthus inversus syndrome: FOXL2 is a conserved, early, acting gene in vertebrate ovarian development, Endocrinology 144, 3237-3243.
Afonso, L. O. B., Wassermann, G. J., de Oliveira, R. T., 2001. Sex reversal in nile tilapia (Oreochromis niloticus) using a nonsteroidal aromatase inhibitor. J. Exp. Zool. 290, 177-181.
Lynch, J. P., Lala, D. S., Peluso, J. J., Luo, W., Parker, K. L., White, B. A., 1993. Steroidogenic factor 1, an orphan nuclear receptor, regulates the expressionof the rat aromatase gene in gonadal tissues. Mol. Endocrinol. 7, 776-786.
Lynn, S. G., Birge, W. J., Shepherd, B. S., 2008. Molecular characterization and sex-specific tissue expression of estrogen receptor α (esr1), estrogen receptor βa(esr2a) and ovarian aromatase (cyp19a1a) in yellow perch (Perca flavescens). Comp. Biochem. Physiol. 149, 126-147.
Makinen, S., Makela, S., Weihua, Z., Warner, M., Rosenlund, B., Salmi, S., Hovatta, O., Gustafsson, J. K., 2001. Localization of oestrogen receptors alpha and beta in human testis, Mol. Hum. Reprod. 7, 497-503.
Maldonado, L. C. T., Piedra, A. L., Mendoza, N. M., Valencia, A. M., Martinez, A. M., Larios, H. M., 2002. Expression profiles of dax1, dmrt1, and sox9 during temperature sex determination in gonads of the sea turtle Lepidochelys Olivacea. General and Comparative Endocrinology 129, 20-26.
Marchand, O., Govoroun, M., D’Cotta, H., McMeel, O., Lareyre, J., Bernot, A., Laudet, V., Guiguen, Y., 2000. DMRT1 expression during gonadal differentiation and spermatogenesis in the rainbow trout, Oncorhynchus mykiss. Biochim. Biophys. Acta 1493, 180-187.
Marlatt, V. L., Martyniuk, C. J., Zhang, D., Xiong, H., Watt, J., Xia, X., Moon, T., Trudeau, V. L., 2008. Auto-regulation of estrogen receptor subtypes and gene expression profiling of 17β-estradiol action in the neuroendocrine axis of male goldfish. Mol. Cell. Endocrinol. 283, 38-48.
Matsuda, M., Nagahama, Y., Shinomiya, A., Sato, T., Matsuda, C., Kobayashi, T., Morrey, C., Shibata, N., Asakawa, S., Shimizu, N., Hori, H., Hamaguchi, S., Sakaizumi, M. 2002. DMY is a Y-specific DM-domain gene required for male development in the medaka fish. Nature 417, 559-563.
Matsuda, M., 2003, Sex determination in fish: lessons from the sex-determining gene of the teleost medaka, Oryzias Latipes development growth & differentiation, 45, 397-403.
Matsuda, M., 2005. Sex determination in the teleost medaka, Oryzias latipes. Annu. Rev. Genet. 39, 293-307.
Matsuda, M., Sato, T., Toyazaki, Y., Nagahama, Y., Hamaguchi, S., Sakaizumi, M., 2003. Oryzias curvinotus has DMY, a gene that is required for male development in the medaka, Oryzias latipes. Zool. Sci. 20, 159-161.
Matsuda, M., Shinomiya, A., Kinoshita, M., Suzuki, A., Kobayashi, T., Paul-Prasanth, B., Lau, E. L., Hamaguchi, S., Sakaizumi, M., Nagahama, Y., 2007. DMY gene induces male development in genetically female (XX) medaka fish. Proc. Natl. Acad. Sci. USA. 104, 3865-3870.
Mayer, L., Overstreet, S., Dyer, C., Propper, C., 2002. Sexually dimorphic expression of steroidogenic factor 1 (SF-1) in developing gonads of the American bullfrog, Rana catesbeiana. Gen. Comp. Endocrinol. 127, 40-47.
McAllister, B. G., Kime, D. E., 2003. Early life exposure to environmental levels of the aromatase inhibitor tributyltin causes masculinisation and irreversible sperm damage in zebrafish. Danio rerio. Aquat. Toxicol. 65, 309-316.
Menuet, A., Le Page, Y., Torres, O., Kern, L., Kah, O., Pakdel, F., 2004. Analysis of the estrogen regulation of the zebrafish estrogen receptor (ER) reveals distinct effects of ER alpha, ER beta1 and ER beta2. J. Mol. Endocrinol. 32, 975-986.
Mi, Y., Zhang, C., Xie, M., Zeng, W., 2004. Effects of follicle- stimulating hormone and androgen on proliferation of cultured testicular germ cells of embryonic chickens. Gen. Comp. Endocrinol. 138, 237-246.
Miura, T., Miura, C., 2001. Japanese eel: a model for analysis of spermatogenesis. Zoolog. Sci. 18, 1055-1063.
Miura, T., Miura, C., Ohta, T., Nader, M. R., Todo, T., Yamauchi, K., 1999. Estradiol-17β stimulates the renewal of spermatogonial stem cells in males. Biochem. Biophys. Res. Commun. 264, 230-234.
Miura, T., Yamauchi, K., Takahashi, H., Nagahama, Y., 1991. Hormonal induction of all stages of spermatogenesis in vitro in the male Japanese eel, Anguilla japonica. Proc. Natl. Acad. Sci. USA. 88, 5774-5778.
Miyake, A., Saito, T., Kashiwagi, T., Ando, D., Yamamoto, A., Suzuki, T., Nakatsuji, N., Nakatsuji, T., 2006. Cloning and pattern of expression of the shiro-uo vasa gene during embryogenesis and its roles in PGC development. Int. J. Dev. Biol. 50, 619-625.
Miyata, S., Kubo, T., 2000. In vitro effects of estradiol and aromatase inhibitor treatment on sex differentiation in Xenopus laevis gonads. Gen. Comp. Endocrinol. 119, 105-110.
Moriceau-Hay, D., Doerr-Schott, J., Dubois, M. P., 1982. Immunohistochemical demonstration of TSH, LH and ACTH cells in the hypophysis of tadpoles of Xenopus laevis. Cell Tissue Res. 225, 57-64.
Morrey, C. E., Nakamura, M., Kobayashi, T., Grau, E. G., Nagahama, Y., 1998. P450scc-like immunoreactivity throughout gonadal restructuring in the protogynous hermaphrodite Thalassoma duperrey. Int. J. Dev. Biol. 42, 811-816.
Murata, R., Karimata, H., Alam, M. A., Nakamura, M., 2009. Gonadal sex differentiation in the Malabar grouper, Epinephelus malabaricus. Aquaculture 293, 286-289.
Murdock, C., Wibbels, T., 2003. Expression of Dmrt1 in a turtle with temperature-dependent sex determination. Cytogenet. Genome Res. 101, 302-308.
Muriach, B., Cerda-Reverter, J. M., Gomez, A., Zanuy, S., Carrillo, M., 2008. Molecular characterization and central distribution of the estradiol receptor alpha (ERα) in the sea bass (Dicentrarchus labrax). J. Chem. Neuroanat. 35, 33-48.
Muriach, B., Carrillo, M., Zanuy, S., Cerdá-Reverter, J. M., 2008. Distribution of estrogen receptor 2 mRNAs (Esr2a and Esr2b) in the brain and pituitary of the sea bass (Dicentrarchus labrax). Brain Res. 1210, 126-141.
Nagahama, Y., 2005. Molecular mechanisms of sex determination and gonadal sex differentiation in fish. Fish Physiol. Biochem. 31, 105-109.
Nagahama, Y., Yoshikuni, M., Yamashita, M., Tokumoto, T., Katsu, Y., 1995. Regulation of oocyte growth and maturation in fish. Curr. Top. Dev. Biol. 30, 103-145.
Nagler, J. J., Cavileer, T., Sullivan, J., Cyr, D. G., Rexroad, C., 2007. The complete nuclear estrogen receptor family in the rainbow trout: discovery of the novel ERα2 and both ERβ isoforms, Gene 392, 164-173.
Nagler, J. J., Krisfalusi, M., Cyr, D. G., 2000. Quantification of rainbow trout (Oncorhynchus mykiss) estrogen receptor-alpha messenger RNA and its expression in the ovary during the reproductive cycle. J. Mol. Endocrinol. 25, 243-251.
Nakamoto, M., Matsuda, M., Wang, D. S., Nagahama, Y., Shibata, N., 2007. Molecular cloning and analysis of gonadal expression of Foxl2 in the medaka, Oryzias latipes. Biochem. Biophys. Res. Commun. 344, 353-361.
Nakamura, M., Bhandari, R. K., Higa, M., 2003. The role estrogens play in sex differentiation and sex changes of fish. Fish Physiol. Biochem. 28, 113-117.
Nakamura, M., Takahashi, H., 1973. Gonadal sex differentiation in Tilapia mossambica, with special regard to the time of estrogen treatment effective in inducing complete feminization of genetic males. Bull. Fac. Fish. Hokkaido Univ. 24, 1-13.
Nakamura, M., Hourigan, T. F., Yamauchi, K., Nagahama, Y., Grau, E. G., 1989. Histological and ultrastructural evidence for the role of gonadal steroid hormones in sex change in the protogynous wrasse, Thalassoma duperrey. Environ. Biol. Fish 24, 117-136.
Nanda, I., Kondo, M., Hornung, U., Asakawa, S., Winkler, C., Shimizu, A., Shan, Z., Haaf, T., Shimizu, N., Shima, A., Schmid, M., Schartl, M., 2002. A duplicated copy of DMRT1 in the sex-determining region of the Y chromosome of the medaka, Oryzias latipes. Proc. Natl. Acad. Sci. USA. 99, 11778-11783.
Nanda, I., Zend-Ajusch, E., Shan, Z., Grutzner, F., Schartl, M., Burt, D. W., Koehler, M., Fowler, V. M., Goodwin, G., Schneider, W. J., Mizuno, S., Dechant, G., Haaf, T., Schmid, M., 2000. Conserved synteny between the chicken z sex chromosome and human chromosome 9 includes the male regulatory gene Dmrt1: a comparative review on Avian sex determination. Cytogenetics and Cell Genetics 89, 67-78.
Nocillado, J. N., Elizur, A., Avitan, A., Carrick, F., Levavi-Sivan, B., 2007. Cytochrome P450 aromatase in grey mullet: cDNA and promoter isolation; brain, pituitary and ovarian expression during puberty. Mol. Cell. Endocrinol. 263, 65-78.
Nozu, R., Kojima, Y., Nakamura, M., 2009. Short term treatment with aromatase inhibitor induces sex change in the protogynous wrasse, Halichoeres trimaculatus. Gen. Comp. Endocrinol. 161, 360-364.
Ogawa, K., Suzuki, E., Taniguchi, K., 1995. Immunohistochemical studies on the development of the hypothalamo-hypophysial system in Xenopus laevis. Anat. Rec. 241, 244-254.
Olsen, L., Aasland, R., Fjose, A., 1997. A vasa-like gene in zebrafish identifies putative primordial germ cells. Mech. Dev. 66, 95-105.
Orlando, E. F., Guillette Jr, L. J., 2007. Sexual dimorphic responses in wildlife exposed to endocrine disrupting chemicals. Environ. Res. 104, 163-173.
Otani, S., Maegawa, S., Inoue, K., Arai, K., Yamaha, E., 2002. The germ cell lineage identified by vasa-mRNA during the embryogenesis in goldfish. Zool. Sci. 19, 519-526.
Ottolenghi, C., Veitia, R., Quintana-Murci, L., Torchard, D., Scapoli, L., Souleyreau-Therville, N., Beckmann, J., Fellous, M. Mcelreavey, K., 2000. The region on 9p associated with 46, xy sex reversal contains several transcripts expressed in the urogenital system and a novel doublesex-related domain. Genomics 64, 170-178.
Pandolfi, M., Lo Nostro, F. L., Shimizu, A., Pozzi, A. G., Meijide, F. J., Vazquez, G. R., Maggese, M. C., 2006. Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation. Anat. Embryol. (Berl) 211, 355-365.
Pannetier, M., Servel, N., Cocquet, J., Besnard, N., Cotinot, C., Pailhoux, E., 2003. Expression studies of the PIS-regulated genes suggest different mechanisms of sex determination within mammals, Cytogenet. Genome. Res. 101, 199-205.
Park, S. Y., Jameson, J. L., 2005. Minireview: transcriptional regulation of gonadal development and differentiation. Endocrinology 146, 1035- 1042.
Paul-Prasanth, B., Matsuda, M., Lau, E. L., Suzuki, A., Sakai, F., Kobayashi, T., Nagahama, Y., 2006. Knock-down of DMY initiates female pathway in the genetic male medaka, Oryzias latipes. Biochem. Biophys. Res. Commun. 351, 815-819.
Pelletier, G., Labrie, C., Labrie, F., 2000. Localization of oestrogen receptor α, oestrogen receptor β and androgen receptors in the rat reproductive organs. J. Endocrinol. 165, 359-370.
Pepe, G. J., Billiar, R. B., Leavitt, M. G., Zachos, N. C., Gustafsson, J., Albrecht, E. D., 2002. Expression of estrogen receptors α and β in the baboon fetal ovary. Biol. Reprod. 66, 1054-1060.
Pieau, C., Dorizzi, M., 2004. Estrogens and temperature-dependent sex determination in reptiles: all is in the gonads. J. Endocrinol. 181, 367-377.
Piferrer, F., Baker, I. J., Donaldson, E. M., 1993. Effects of natural, synthetic, aromatizable, and nonaromatizable androgens in inducing male sex differentiation in genotypic female Chinook salmon (Oncorhynchus tshawytscha). Gen. Comp. Endocrinol. 91, 59-65.
Piferrer, F., Zanuy, S., Carillo, M., Solar, I. I., Devlin, R. H., Donaldson, E. M., 1994. Brief treatment with an aromatase inhibitor during sex differentiation cause chromosomally female salmone to develop as normal functional males. J. Exp. Zool. 270, 255-262.
Piferrer, F., 2001. Endocrine sex control strategies for the feminization of teleost fish. Aquaculture 197, 229-281.
Piferrer, F., Blazquez, M., Navarro, L., Gonzalez, A., 2005. Genetic, endocrine, and environmental components of sex determination and differentiation in the European sea bass (Dicentrarchus labrax L.). Gen. Comp. Endocrinol. 142, 102-110.
Pilon, N., Behdjani, R., Daneau, I., Lussier, J., Silversides, D., 1998. Porcine steroidogenic factor-1 gene (pSF-1) expression and analysis of embryonic pig gonads during sexual differentiation. Endocrinology 139, 3803-3812.
Raghuveer, K., Senthilkumaran, B., 2010. Cloning and differential expression pattern of vasa in the developing and recrudescing gonads of catfish, Clarias gariepinus.Comp. Biochem. Physiol. A 157, 79-85.
Rasheeda, M. K., Sridevi, P., Senthilkumaran, B., 2010. Cytochrome P450 aromatases: impact on gonadal development, recrudescence and effect of hcg in the catfish, Clarias gariepinus. Gen. Comp. Endocrinol. 167, 234-245.
Raucci, F., DiFiore, M. M., 2007. The c-kit receptor protein in the testis of green frog Rana esculenta: seasonal changes in relationship to testosterone titres and spermatogonial proliferation. Reproduction 133, 51-60.
Raymond, C. S., Murphy, M. W., O'Sullivan, M. G., Bardwell, V. J., Zarkower, D., 2000. Dmrt1, a gene related to worm and fly sexual regulators, is required for mammalian testis differentiation. Genes &Development, 14, 2587-2595.
Raymond, C., Kettlewell, J., Hirsch, B., Bardwell, V., Zarkower, D., 1999a. Expression of Dmrt1 in the genital ridge of mouse and chicken embryos suggests a role in vertebrate sexual development. Devel. Biol. 215, 208-220.
Raymond, C., Parker, E., Kettlewell, J., Brown, L., Page, D., Kusz, K., Jaruzelska, J., 1999b. A region of human chromosome 9p required for testis development contains two genes related to known sexual regulators. Hum. Molec. Genet. 8, 989-996.
Rocha, A., Zanuy, S., Carrillo, M., Gomez, A., 2009. Seasonal changes in gonadal expression of gonadotropin receptors, steroidogenic acute regulatory protein and steroidogenic enzymes in the European sea bass. Gen. Comp. Endocrinol. 162, 265-275.
Roselli, C. E., Jorgensen, E. Z., Doyle, M. W., Ronnekleiv, O. K., 1997. Expression of the orphan receptor steroidogenic factor-1 mRNA in the rat medial basal hypothalamus. Brain Res. Mol. Brain Res. 44, 66-72.
Sabo-Attwood, T., Kroll, K. J., Denslow, N. D., 2004. Differential expression of largemouth bass (Micropterus salmoides) estrogen receptor isotypes alpha, beta, and gamma by estradiol. Mol. Cell. Endocrinol. 218, 107-118.
Sadovy, Y., Shapiro, D. Y., 1987. Criteria for the diagnosis of hermaphroditism in fishes. Copeia 1, 136-156.
Sadovy, Y. J., Donaldson, T. J., Graham, T. R., McGilvray, F., Muldoon, G. J., Phillips, M. J., Rimmer, M. A., Smith, A., Yeeting, B., 2003. While Stocks Last: The Live Reef Food Fish Trade. Asian Development Bank, Manila, Philippines, pp. 8-19.
Saito, D., Morinaga, C., Aoki, Y., Nakamura, S., Mitani, H., Furutani-Seiki, M., Kondoh, H., Tanaka, M., 2007. Proliferation of germ cells during gonadal sex differentiation in medaka: insights from germ cell-depleted mutant zenzai. Dev. Biol. 310, 280-290.
Saito, J., Otani, S., Fujimoto, T., Suzuki, T., Nakatsuji, T., Arai, K., Yamaha, E., 2004. The germ line lineage in ukigori, Gymnogobius species (Teleostei: Gobiidae) during embryonic development. Int. J. Dev. Biol. 48, 1079-1085.
Sawyer, S., Gerstner, K. A., Callard G. V., 2006. Real-time PCR analysis of cytochrome P450 aromatase expression in zebrafish: gene specific tissue distribution, sex differences, developmental programming and estrogen regulation. Gen. Comp. Endocrinol. 147, 108-117.
Schlegel, A., Wang, C., Pestell, R. G., Lisanti, M. P., 2001. Ligand- independent activation of oestrogen receptor alpha by caveolin-1. Biochem. J. 359, 203-210.
Shen, W. H., Moore, C. C., Ikeda, Y., Parker, K. L., Ingraham, H. A., 1994. Nuclear receptor steroidogenic factor 1 regulates the mullerian inhibiting substance gene: a link to the sex determination cascade. Cell 77, 651-661.
Shibata, N., Hamaguchi, S., 1988. Evidence for the sexual bipotentiality of spermatogonia in the fish, Oryzias latipes. J. Exp. Zool. 245, 71-77.
Shibata, K., Takase, M., Nakamura, M., 2002. The Dmrt1 Expression in sex-reversed gonads of amphibians. General and Comparative Endocrinology 127, 232-241.
Shinomiya, A., Tanaka, M., Kobayashi, T., Nagahama, Y., Hamaguchi, S., 2000. The vasa-like gene, olvas, identifies the migration path of primordial germ cells during embryonic body formation stage in the medaka, Oryzias latipes. Dev. Growth Diff. 42, 317-326.
Siegfried, K. R., Nusslein-Volhard, C., 2008. Germline control of female sex determinationin zebrafish. Dev. Biol. 324, 277-287.
Siggers, P., Lee, S., Greenfield, A., 2002. Sexually dimorphic expression of Gata-2 during mouse gonad development. Mech. Dev. 111, 159-162.
Simoni, M., Gromoll, J., Hoppner, W., Nieschlag, E., 1997. Molecular pathophysiology of the pituitary-gonadal axis. Adv. Exp. Med. Biol. 424, 89-97.
Sinclair, A., Berta, P., Palmer, M., Hawkins, J., Griffiths, B., Smith, M., Foster, J., Frischauf, A., Lovell-Badge, R., Goodfellow, P., 1990. A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif. Nature 346, 240-244.
Slanchev, K., Stebler, J., de la Cueva-Mendez, G., Raz, E., 2005. Development without germ cells: the role of the germ line in zebrafish sex differentiation. Proc. Natl. Acad. Sci. USA. 102, 4074-4079.
Smith, C. A., Sinclair, A. H., 2004. Sex determination: insights from the chicken. BioEssays 26, 120-132.
Smith, C. L., 1965. The pattern of sexuality and the classification of serranid fishes. Am. Mus. Novit. 2207, 1-20.
Smith, C., McClive, P., Western, P., Reed, K., Sinclair, A., 1999a. Conservation of a sex-determining gene. Nature 402, 601-602.
Smith, C., Smith, M., Sinclair, A., 1999b. Expression of chicken steroidogenic factor-1 during gonadal sex differentiation. Gen. Comp. Endocrinol. 113, 187-196.
Stacey, N., Kobayashi, M., 1996. Androgen induction of male sexual behaviors in female goldfish. Horm. Behav. 30, 434-445.
Sunobe, T., Nakamura, M., Kobayashi, Y., Kobayashi, T., Nagahama, Y., 2005. Aromatase immunoreactivity and the role of enzymes in steroid pathways for inducing sex change in the hermaphrodite gobiid fish Trimma okinawae. Comp. Biochem. Physiol. Part A Mol. Integr. Physiol. 141: 54-59.
Suzuki, A., Tanaka, M., Shibata, N., 2004. Expression of aromatase mRNA and effects of aromatase inhibitor during ovarian development
in the medaka, Oryzias latipes. J. Exp. Zool. 301, 266-273.
Sugawara, T., Holt, J. A., Kiriakidou, M., Strauss 3rd, J. F., 1996. Steroidogenic factor 1-dependent promoter activity of the human steroidogenic acute regulatory protein (StAR) gene. Biochemistry 35, 9052-9059.
Swain, A., Narvaez, V., Burgoyne, P., Camerino, G., Lovell-Badge, R., 1998. Dax1 antagonizes Sry action in mammalian sex determination. Nature 391, 761-767.
Swanson, P., Dickey, J. T., Campbell, B., 2003. Biochemistry and physiology of fish gonadotropins. Fish Physiol. Biochem. 28, 53-59.
Tan, S. M., Tan, K. S., 1974. Biology of the tropical grouper Epinephelus tauvina (Forskal) I. A preliminary study on hermaphroditism in E. tauvina. Sing. J. Prim. Ind. 2, 123-133.
Tchoudakova, A., Kishida, M., Wood, E., Callard, G. V., 2001. Promoter characteristics of two cyp19 genes differentially expressed in the brain and ovary of teleost fish. J. Steroid Biochem. Mol. Biol. 78, 427-439.
Tevosian, S. G., Albrecht, K. H., Crispino, J. D., Fujiwara, Y., Eicher, E. M., Orkin., S. H., 2002. Gonadal differentiation, sex determination and normal Sry expression in mice require direct interaction between transcription partners GATA4 and FOG2. Development 129, 4627- 4634.
Tong, S. K., Chung, B. C., 2003. Analysis of zebrafish cyp19 promoters. J. Steroid Biochem. Mol. Biol. 86, 381-386.
Trant, J. M., Gavassa, S., Ackers, J., Chung, B. C., Place, A. R., 2001. Developmental expression of cytochrome P450 aromatase genes (CYP19a and CYP19b) in zebrafish fry, Danio rerio. J. Exp. Zool. 290, 475-483.
Tremblay, A., Tremblay, G. B., Labrie, F., Giguere, V., 1999. Ligand- independent recruitment of SRC-1 to estrogen receptor β through phosphorylation of activation function AF-1. Mol. Cell 3, 513-519.
Tremblay, J., Viger, R., 2001. Nuclear Receptor Dax-1 Represses the Transcriptional Cooperation Between GATA-4 and SF-1 in Sertoli Cells. Biol. Reprod. 64, 1191-1199.
Tsai, C. L., Chang, S. L., Wang, L. H., Chao, T. Y., 2003. Temperature influences the ontogenetic expression of aromatase and oestrogen receptor mRNA in the developing tilapia (Oreochromis mossambicus) brain. J. Neuroendocrinol. 15, 97-102.
Tsai, Y. J., Lee, M. F., Chen, J. Y., Chang, C. F., 2011. Development of gonadal tissue and aromatase function in the protogynous orange- spotted grouper, Epinephelus coioides. Zool. Stud. 50, 693-704.
Uchida, D., Yamashita, M., Kitano, T., Iguchi, T., 2004. An aromatase inhibitor or high water temperature induce oocyte apoptosis and depletion of P450 aromatase activity in the gonads of genetic female zebrafish during sex-reversal. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 137, 11-20.
Uhlenhaut N. Henriette, Susanne Jakob, Katrin Anlag, Tobias Eisenberger, Ryohei Sekido, Jana Kress, Anna-Corina Treier, Claudia Klugmann, Christian Klasen, Nadine I. Holter, Dieter Riethmacher, Günther Schütz, Austin J. Cooney, Robin Lovell-Badge, Mathias Treier., 2009. Somatic Sex Reprogramming of Adult Ovaries to Testes by FOXL2 Ablation. Cell 139, 1130-1142.
Urbatzka, R., Lorenz, C., Lutz, I., Kloas, W., 2010. Expression profiles of LHβ, FSHβ and their gonadal receptor mRNAs during sexual differentiation of Xenopus laevis tadpoles. Gen. Comp. Endocrinol. 168, 239-244.
Urbatzka, R., Lutz, I., Kloas, W., 2007. Aromatase, steroid-5-alpha- reductase type 1 and type 2 mRNA expression in gonads and in brain of Xenopus laevis during ontogeny. Gen. Comp. Endocrinol. 153, 280-288.
Vinas, J., Piferrer, F., 2008. Stage-specific gene expression during fish spermatogenesis as determined by laser-capture microdissection and quantitative-PCR in sea bass (Dicentrarchus labrax) gonads. Biol. Reprod. 79, 738-747.
Vischer, H. F., Bogerd, J., 2003. Cloning and functional characterization of a gonadal luteinizing hormone receptor complementary DNA from the African catfish, Clarias gariepinus. Biol. Reprod. 68, 262-271.
Vizziano, D., Baron, D., Randuineau, G., Mahe, S., Cauty, C., Guiguen, Y., 2008. Rainbow trout gonadal masculinization induced by inhibition of estrogen synthesis is more physiological than masculinization induced by androgen supplementation. Biol. Reprod. 78, 939-946.
Vizziano, D., Randuineau, G., Baron, D., Cauty, C., Guiguen, Y., 2007. Characterization of early molecular sex differentiation in rainbow trout, Oncorhynchus mykiss. Dev. Dyn. 236, 2198-2206.
Volff, J. N., Kondo, M., Schartl, M., 2003. Medaka dmY/dmrt1Y is not the universal primary sex-determining gene in fish. Trends Genet. 19, 196-199.
Von Hofsten, J., Olsson, P. E., 2005. Zebrafish sex determination and differentiation: involvement of Ftz-F1 genes. Reprod. Biol. Endocrinol. 3, 63-73.
Von Schalburg, K. R., Rise, M. L., Brown, G. D., Davidson, W. S., Koop, B. F., 2005. A comprehensive survey of the genes involved in maturation and development of the rainbow trout ovary. Biol. Reprod. 72, 687-699.
Wakabayashi, N., Suzuki, A., Hoshino, H., Nishimori, K., Mizuno, S., 1997. The cDNA cloning and transient expression of a chicken gene encoding a follicle-stimulating hormone receptor. Gene 197, 121-127.
Wang, D. S., Kobayashi, T., Zhou, L.Y., Paul-Prasanth, B., Ijiri, S., Sakai, F., Okubo, K., Morohashi, K. I., Nagahama, Y., 2007. Foxl2 up- regulates aromatase gene transcription in a female-specific manner by binding to the promoter as well as interacting with Ad4BP/SF-1. Mol. Endocrinol. 21, 712-725.
Wang, D. S., Kobayashi, T., Zhou, L., Nagahama, Y., 2004. Molecular cloning and gene expression of Foxl2 in the Nile tilapia, Oreochromis niloticus. Biochem. Biophys. Res. Commun. 320, 83-89.
Wang, D. S., Kobayashi, T., Zhou, L.Y., Ijiri, S., Morohashi, K., Nagahama, Y., 2006. Transcriptional regulation of aromatase (Cyp19a1) by Foxl2 and Dmrt1 in the gonads during sex differentiation in the Nile tilapia, Oreochromis niloticus. In: The 4th International Symposium on the Biology of Vertebrate Sex Determination. Hawaii, USA. pp. 35-36.
Wang, X. G., Bartfai, R., Sleptsova-Freidrich, I., Orban, L., 2007. The timing and extent of “juvenile ovary” phase are highly variable during zebrafish testis differentiation. J. Fish. Biol. 70, 33-44.
Wang, D. S., Zhou, L. Y., Kobayashi, T., Matsuda, M., Shibata, Y., Sakai, F., Nagahama, Y., 2010. Doublesex-and mab-3-related transcription factor- repression of aromatase transcription, a possible mechanism favoring the male pathway in tilapia. Endocrinology 151, 1331-1340.
Wang, Z., Jeffs, B., Ito, M., Achermann, J., Yu, R., Hales, D., Jameson, J. 2001. Aromatase (Cyp19) expression is up-regulated by targeted disruption of Dax1. Proc. Natl. Acad. Sci. USA 98, 7988-7993.
Watanabe, M., Tanaka, M., Kobayashi, D., Yoshiura, Y., Oba, Y., Nagahama, Y., 1999. Medaka (Oryzias latipes) FTZ-F1 potentially regulates the transcription of P-450 aromatase in ovarian follicles: cDNA cloning and functional characterization. Mol. Cell. Endocrinol. 149, 221-228.
Wennstrom, K. L., and Crews, D., 1995. Making males from females: The effects of aromatase inhibitors on a parthenogenetic species of whiptail lizard. Gen. Comp. Endocrinol. 99, 316-322.
Western, P., Harry, J., Marshal-Graves, J., Sinclair, A., 2000. Temperature-dependent sex determination in the American alligator: expression of SF1, WT1 and DAX1 during gonadogenesis. Gene 241, 223-232.
Wibbels, T., Cowan, J., LeBoeuf, R., 1998. Temperature-dependent sex determination in the red-eared slider turtle, Trachemys scripta. J. Exp. Zool. 281, 409-416.
Williamson, A., Lehmann, R., 1996. Germ cell development in Drosophila. Annu. Rev. Cell Dev. Biol. 12, 365-391.
Wong, T. T., Gothilf, Y., Zmora, N., Kight, K. E., Meiri, I., Elizur, A., Zohar, Y., 2004. Developmental expression of three forms of gonadotropin-releasing hormone and ontogeny of the hypothalamic- pituitary-gonadal axis in gilthead seabream (Sparus aurata). Biol. Reprod. 71, 1026-1035.
Wu, G. C., Tomy, S., Nakamura, M., Chang, C. F., 2008. Dual roles of cyp19a1a in gonadal sex differentiation and development in the protandrous black porgy, Acanthopagrus schlegeli. Biol. Reprod. 79, 1111-1120.
Wu, G. C., Chiu, P. C., Lin, C. J., Lyu, Y. S., Lan, D. S., Chang, C. F., 2011. Testicular dmrt1 is involved in the sexual fate of the ovotestis in the protandrous black porgy. Biol. Reprod. (SCI, in press)
Wu, C., Patiňo, R., Davis, K. B., Chang, X., 2001. Localization of estrogen receptor α and β RNA in germinal and nongerminal epithelia of the channel catfish testis. Gen. Comp. Endocrinol. 124, 12-20.
Xia, W., Zhou, L., Yao, B., Li, C. J., Gui, J. F., 2007. Differential and spermatogenic cell-specific expression of dmrt1 during sex reversal in protogynous hermaphroditic groupers. Mol. Cell. Endocrinol. 263, 156-172.
Xu, J., Li, Q., 2003. Review of the in vivo functions of the p160 steroid receptor coactivator family. Mol. Endocrinol. 17, 1681-1692.
Xu, H., Gui, J., Hong, Y., 2005. Differential expression of vasa RNA and protein during spermatogenesis and oogenesis in the gibel carp (Carassius auratus gibelio), a bisexually and gynogenetically reproducing vertebrate. Dev. Dyn. 233, 872-882.
Yamaguchi, A., Lee, K. H., Fujimoto, H., Kadomura, K., Yasumoto, S., Matsuyama, M., 2006. Expression of DMRT1 gene and its roles in early gonadal development of Japanese pufferfish, Takifugu rubripes. Comp. Biochem. Physiol. Part D Genomics Proteomics 1, 59-68.
Yamaguchi, T., Yamaguchi, S., Hirai, T., Kitano, T., 2007. Follicle- stimulating hormone signaling and Foxl2 are involved in transcriptional regulation of aromatase gene during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus. Biochem. Biophys. Res. Commun. 359, 935-940.
Yamamoto, T., 1969. Sex differentiation. In: Hoar, W. S., Randall, D. J. (Eds.), Fish Physiology, vol. 3. Academic Press, London, pp. 117-175.
Yao, H. H., 2005. The pathway to femaleness: current knowledge on embryonic development of the ovary. Mol. Cell. Endocrinol. 230, 87-93.
Yao, H. H., Matzuk, M. M., Jorgez, C. J., Menke, D. B., Page, D. C., Swain, A., Capel, B., 2004. Follistatin operates downstream of Wnt4 in mammalian ovary organogenesis. Dev. Dyn. 230, 210-215.
Yeh, S. L., Kuo, C. M., Ting, Y. Y., Chang, C. F., 2003. Androgens stimulate sex change in protogynous grouper, Epinephelus coioides: spawning performance in sex-changed males. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 135, 375-382.
Yeh, S., L, Y. Y., Ting, C., Kuo, M., 1989. Technique of pellet implantation and preparation for induced sex reversal of the grouper Epinephelus salmonoides, Epinephelus fario. Bull. Taiwan Fish. Res. Inst. 47, 213-219.
Yoon, C., Kawakami, K., Hopkins, N., 1997. Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell-stage embryos and is expressed in the primordial germ cells. Development 124, 3157-3165.
Yoshiura, Y., Senthilkumaran, B., Watanabe, H., Oba, Y., Kobayashi, T., Nagahama, Y., 2003. Synergistic expression of Ad4BP/SF-1 and cytochrome P-450 aromatase (ovarian type) in the ovary of Nile tilapia, Oreochromis niloticus, during vitellogenesis suggests transcriptional interaction. Biol. Reprod. 68, 1545-1553.
Young, G., Kusakabe, M., Nakamura, I., 2005. Gonadal steroidogenesis in teleost fish. In: Melamed P, Sherwood N, editors.Hormones and their receptors in fish reproduction. Hackensack, N. J., World Scientific Publishing Co. Pte. Ltd. p 155-223.
Yu, N.W., Hsu, C.Y., Ku, H.H., Chang, L.T., Liu, H.W., 1993. Gonadal differentiation and secretions of estradiol and testosterone of the ovaries of Rana catesbeiana tadpoles treated with 4-hydroxyandrostenedione.J. Exp. Zool. 265, 252-257.
Zhang, W., Zhang, Y., Zhang, L., Zhao, H., Li, X., Huang, H., Lin, H., 2007a. The mRNA expression of P450 aromatase, gonadotropin b-subunits and FTZ-F1 in the orange-spotted grouper (Epinephelus coioides) during 17α-methyltestosteroneinduced precocious sex change. Mol. Reprod. Dev. 74, 665-673.
Zhang, W., Zhang, Y., Zhang, L., Zhao, H., Li, X., Huang, H., Lin, H., 2007b. The mRNA expression of P450 aromatase, gonadotropin beta- subunits and FTZ-F1 in the orange-spotted grouper, Epinephelus coioides, during 17alpha-methyltestosterone-induced precocious sex change. Mol. Reprod. Dev. 74, 665-673.
Zhang, Y., Zhang, W., Zhang, L., Zhang, L., Zhu, T., Tian, J., Li, X., Lin, H., 2004. Two distinct cytochrome P450 aromatases in the orange-spotted grouper, Epinephelus coioides: cDNA cloning and differential mRNA expression. J. Steroid Biochem. Mol. Biol. 92, 39-50.
Zhou, L., Gui, J. F., 2010. Molecular mechanisms underlying sex change in hermaphroditic groupers. Fish Physiol. Biochem. 36, 181-193.
Zhu, P., Zhang, Y., Zhuo, Q., Lu, D., Huang, J., Liu, X., Lin, H., 2008. Discovery of four estrogen receptors and their expression profiles during testis recrudescence in male Spinibarbus denticulatus. Gen. Comp. Endocrinol. 156, 265-276.

連結至畢業學校之論文網頁點我開啟連結
註: 此連結為研究生畢業學校所提供,不一定有電子全文可供下載,若連結有誤,請點選上方之〝勘誤回報〞功能,我們會盡快修正,謝謝!
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔