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研究生:許育珺
研究生(外文):Yu-Chun Hsu
論文名稱:褐藻藻黃素對 Streptozotocin-Nicotiamide 誘發糖尿病雄性大鼠生殖功能之影響
論文名稱(外文):Effects of Fucoxanthin on Male Reproductive Function of Rats with Streptozotocin-Nicotiamide Induced Diabetes
指導教授:龔瑞林龔瑞林引用關係
指導教授(外文):Zwe-Ling Kong
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:食品科學系
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:138
中文關鍵詞:藻黃素糖尿病不孕症男性生殖性腺
外文關鍵詞:FucoxanthinDiabetesMale reproductionHypogonadismSpermatogenesis
相關次數:
  • 被引用被引用:7
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  • 下載下載:22
  • 收藏至我的研究室書目清單書目收藏:0
糖尿病造成男性不孕的發生,主因為氧化壓力及生殖內分泌紊亂,導致精子受損及性腺功能異常。本研究以藻黃素探討糖尿病造成男性生殖功能損害之保護功效。細胞實驗以 RAW264.7 小鼠巨噬細胞測試 97% 藻黃素之抗發炎、抗氧化能力。藻黃素在最高濃度 6.25 g/mL RAW264.7 不會造成毒性,且可降低 NO 釋放及清除 ROS,達到抗氧化及抗發炎之作用。動物實驗以 nicotiamide (230 mg/kg) 及 strepzotocin (65 mg/kg) 誘導糖尿病雄性大鼠,探討氧化壓力,內分泌調節及造精作用。實驗分組:控制組、糖尿病組、糖尿病管餵 rosiglitazone (RSG) 0.571 mg/kg、40% 藻黃素 13、26、65 mg/kg,為期 2 週與 4 週。2 週結果顯示:餵食 26、65 mg/kg下明顯改善血糖、胰島素阻抗,進而調節 KiSS1、GPR54 mRNA 表現量,增加 LH 及睪固酮的分泌,改善內分泌調節及造精作用正常;並且改善脂質過氧化,增加抗氧化酵素之活性,及O2- 的清除能力,減少氧化壓力。4週結果顯示:餵食任何劑量下可改善胰島素及瘦素阻抗導致之內分泌紊亂及造精作用異常;降低氧化壓力減少精子受損。本研究證實,補充藻黃素藉由改善糖尿病雄性大鼠之內分泌異常及氧化壓力,達到保護生殖能力的作用,未來在改善男性糖尿病不孕症具有潛力。
Hypogonadism and oxidative stress occurs commonly in men with type 2 diabetes associated male infertility. The aim of this study was to investigate the capability of anti-inflammatory and anti-oxidative on fucoxanthin, and to evaluate the protective effects on male reproduction of fucoxanthin against diabetic rats. In vitro, concentration 6.25 g/mL of 97% fucoxanthin significantly inhibited NO production, ,reactive oxidative spices production in lipopolysaccharide (LPS) induced RAW 264.7 macrophage cells. In animal experiment, the model diabetic rat was induced by nicotinamide (230 mg/kg) and streptozotocin (65 mg/kg). 40% fucoxanthin was tested in three doses (13, 26 and 65 mg/kg, p.o daily) for 2 and 4 week. Besides, rosiglitazone (RSG) administration (0.571 mg/kg) as positive control. The results indicate that any dose of fucoxanthin administration for 2 weeks improve hyperglycemia and insulin resistance, sperm numbers, motivity and lipid peroxidation. But only 26 and 65 mg/kg of fucoxanthin could significantly restore KiSS1, GPR54 mRNA expression, activity of anti-oxidative enzyme, scavenging free radical. Any dose of fucoxanthin administration for 4 weeks improve insulin resistance and leptin resistance, and restored sperm motivity, decreased abnormal sperm number, inhibit lipid peroxidative. Besides,it has restored GPR54 and SOCS-3 mRNA expression in the hypothalamus, and recover LH and testosterone level. In conclusion, not only the antioxidant and antiinflammatory property but also steroidogenesis effect of fucoxanthin might have contributed for its ability to decrease the type-2 diabetes mediated insulin and leptin resistance, and protect male reproduction.
中文摘要………………………………………………………………..I
Abstract………………………………………………………………...II
目錄……………………………………………………………………III
縮寫表………………………………………………………………XIV
前言……………………………………………………………………..1
第一章、文獻回顧……………………………………………………..3
一、男性不孕症 3
1. 不孕症現狀 3
2. 男性不孕症之診斷 3
3. 男性不孕症分類 4
4. 男性不孕症之成因 5
(1) 糖尿病 5
(2) 肥胖 5
(3) 抽菸 5
(4) 酗酒 6
(5) 睪丸異常 6
(6) 老化 6
(7) 環境荷爾蒙 6
二、糖尿病 7
1. 糖尿病的現況 7
2. 糖尿病的分類 8
3. 糖尿病的診斷標準 11
三、糖尿病與男性生殖 12
1. 流行病學與動物模式研究 12
2. 糖尿病與氧化壓力 13
3. 糖尿病與生殖內分泌異常 15
3.1 男性生殖內分泌調控:下視丘-腦下垂體-性腺調控軸 (hypothalamic-pituitary-gland, HPG axis) 15
3.2 糖尿病對KiSS1/ GPR54系統與生殖內分泌之影響 16
四、藻黃素 (Fucoxanthin) 19
1. 藻黃素簡介 19
2. 藻黃素之毒性 20
3. 藻黃素之功能 21
3.1 抗氧化活性 21
3.2 抗發炎能力 22
3.3 抗癌症功效 22
3.4 抗肥胖功效 23
3.5 控制糖尿病 24
3.6 保護肝臟 24
3.7 皮膚保護作用 25
3.8 抗血管新生作用 26
3.9 腦神經血管保護作用 26
3.10骨骼保護作用 27
第二章、實驗流程…………………………………………………...28
第三章、實驗材料……………………………………………………31
一、 材料來源 31
二、 細胞株 31
三、 實驗動物 31
四、 動物飼料 31
五、 藥品 32
六、 儀器設備 34
第四章、實驗方法…………………………………………………...36
一、 細胞實驗 36
1. 細胞培養 36
2. 細胞存活率測定 (MTT assay) 36
3. 一氧化氮 (Nitric oxide, NO) 釋放量 37
4. 吞噬球呼吸爆 NBT 還原分析法 37
5. 細胞內 ROS 含量 38
二、 動物實驗 39
1. 第二型糖尿病大鼠之誘導與分組 39
2. 實驗動物飼養 40
3. 血液樣品分析 41
3.1 葡萄糖 (glucose) 含量測定 42
3.2 總三酸甘油酯 (total triglyceride) 濃度測定 42
3.3 總膽固醇 (total cholesterol) 濃度測定 43
3.4 胰島素 (insulin) 含量測定 44
3.5 瘦素 (leptin) 含量測定 44
3.6 HOMA-IR 值計算 44
3.7 血漿之脂質過氧化影響 45
3.8 血漿之睪固酮測定 45
3.9 血漿之LH 濃度 46
3.10 TNF-α、IL-6濃度測定 46
4. 精子樣品分析 48
4.1 精子樣品收集 48
4.2 精子數目之計算 48
4.3 具活動力精子之計算 48
4.4 異常精子之計算 49
4.5 精子之脂質過氧化程度 49
4.6 精子之 ROS 含量測定 50
5. 睪丸分析 51
5.1 睪丸之脂質過氧化影響 51
5.2 睪丸中抗氧化酵素測定 52
5.3 睪丸型態之組織冷凍切片及染色 53
6. 下視丘分析 53
6.1 下視丘之 KiSS1、GPR54、SOCS-3 mRNA 表現量 53
第五章、統計分析……………………………………………………56
第六章、結果與討論…………………………………………………57
一、 以體外實驗探討藻黃素對於 RAW264.7 小鼠巨噬細胞之抗發炎及抗氧化功能 57
1. 藻黃素對 RAW264.7 小鼠巨噬細胞存活率探討 57
2. 藻黃素對 RAW264.7 小鼠巨噬細胞 NO 產生量之探討 …………………………………………………………….58
3. 藻黃素對LPS 誘發超氧陰離子之 NBT 還原能力試驗 58
4. 藻黃素抑制胞內 ROS 產生之探討 59
二、 以體內實驗探討藻黃素對於STZ-NA誘導糖尿病雄性大白鼠之生殖功能 60
1. 藻黃素對於糖尿病大鼠葡萄糖耐受性之探討 60
2. 藻黃素對於糖尿病大鼠血漿生化指標濃度之影響 61
2.1 血漿葡萄糖、總膽固醇及三酸甘油酯含量 61
2.2 血漿胰島素、瘦素、胰島素阻抗評估 62
3. 藻黃素對於糖尿病大鼠睪丸與精子之探討 63
3.1 藻黃素對於糖尿病大鼠睪丸、附睪、附睪脂肪重量之影響 63
3.2 藻黃素對於糖尿病大鼠精子參數之影響 63
4. 藻黃素對於糖尿病大鼠生精小管形態及生殖細胞厚度之影響 65
5. 藻黃素對血漿中LH 及睪固酮之影響 66
6. 藻黃素對於糖尿病大鼠下視丘 KiSS1、GPR54 及 SOCS-3 mRNA 表現量之影響 67
7. 藻黃素對於糖尿病大鼠血漿及睪丸中抗氧化酵素的影響 ……………………………………………………………68
8. 藻黃素對於糖尿病大鼠精子中氧化壓力的改善 70
9. 藻黃素對於糖尿病大鼠脂質過氧化之改善 72
10.藻黃素對於糖尿病大鼠發炎之改善 73
第七章、結論…………………………………………………………74
第八章、參考文獻……………………………………………………75
圖表……………………………………………………………………86

Agbaje IM, Rogers DA, McVicar CM, McClure N, Atkinson AB, Mallidis C, Lewis SEM. 2007. Insulin dependant diabetes mellitus: implications for male reproductive function. Hum Reprod 22:1871-1877.
Ahmed MU, Brinkmann Frye E, Degenhardt TP, Thorpe SR, Baynes JW. 1997. N-epsilon-(carboxyethyl)lysine, a product of the chemical modification of proteins by methylglyoxal, increases with age in human lens proteins. Biochem J 324 ( Pt 2):565-570.
Airanthi MKWA, Hosokawa M, Miyashita K. 2011. Comparative Antioxidant Activity of Edible Japanese Brown Seaweeds. J Food Sci 76:C104-C111.
Aitken RJ, Baker MA. 2006. Oxidative stress, sperm survival and fertility control. Molecular and Cellular Endocrinology 250:66-69.
Aitken RJ, Sawyer D. 2002. The human spermatozoon - Not waving but drowning. Advances in Male Mediated Developmental Toxicity 518:85-98.
Akkus I, Kalak S, Vural H, Caglayan O, Menekse E, Can G, Durmus B. 1996. Leukocyte lipid peroxidation, superoxide dismutase, glutathione peroxidase and serum and leukocyte vitamin C levels of patients with type II diabetes mellitus. Clin Chim Acta 244:221-227.
Amaral S, Oliveira PJ, Ramalho-Santos J. 2008. Diabetes and the impairment of reproductive function: possible role of mitochondria and reactive oxygen species. Curr Diabetes Rev 4:46-54.
Amaral SCG, Moreno AJ, Santos MS, Seica R, Ramalho-Santos J. 2006. Effects of hyperglycemia on sperm and testicular, cells of Goto-Kakizaki and Streptozotocin-treated rats. Biol Reprod:135-135.
Bartak V, Josifko M, Horackova M. 1975. Juvenile diabetes and human sperm quality. Int J Fertil 20:30-32.
Bell M, Wang R, Hellstrom WJ, Sikka SC. 1993. Effect of cryoprotective additives and cryopreservation protocol on sperm membrane lipid peroxidation and recovery of motile human sperm. J Androl 14:472-478.
Bener A, Al-Ansari AA, Zirie M, Al-Hamaq AOAA. 2009. Is male fertility associated with type 2 diabetes mellitus? Int Urol Nephrol 41:777-784.
Beppu F, Niwano Y, Tsukui T, Hosokawa M, Miyashita K. 2009. Single and repeated oral dose toxicity study of fucoxanthin (FX), a marine carotenoid, in mice. Journal of Toxicological Sciences 34:501-510.
Bjorbaek C, Elmquist JK, Frantz JD, Shoelson SE, Flier JS. 1998. Identification of SOCS-3 as a potential mediator of central leptin resistance. Mol Cell 1:619-625.
Bruning JC, Gautam D, Burks DJ, Gillette J, Schubert M, Orban PC, Klein R, Krone W, Muller-Wieland D, Kahn CR. 2000. Role of brain insulin receptor in control of body weight and reproduction. Science 289:2122-2125.
Cahill DJ, Wardle PG. 2002. Management of infertility. BMJ 325:28-32.
Castellano JM, Navarro VM, Fernandez-Fernandez R, Roa J, Vigo E, Pineda R, Dieguez C, Aguilar E, Pinilla L, Tena-Sempere M. 2006. Expression of hypothalamic KiSS-1 system and rescue of defective gonadotropic responses by kisspeptin in streptozotocin-induced diabetic male rats. Diabetes 55:2602-2610.
Chandel A, Dhindsa S, Topiwala S, Chaudhuri A, Dandona P. 2008. Testosterone Concentration in Young Patients With Diabetes. Diabetes Care 31:2013-2017.
Chavarro JE, Toth TL, Wright DL, Meeker JD, Hauser R. 2010. Body mass index in relation to semen quality, sperm DNA integrity, and serum reproductive hormone levels among men attending an infertility clinic. Fertil Steril 93:2222-2231.
Choi SK, Park YS, Choi DK, Chang HI. 2008. Effects of Astaxanthin on the Production of NO and the Expression of COX-2 and iNOS in LPS-Stimulated BV2 Microglial Cells. J Microbiol Biotechn 18:1990-1996.
Cocuzza M, Sikka SC, Athayde KS, Agarwal A. 2007. Clinical relevance of oxidative stress and sperm chromatin damage in male infertility: an evidence based analysis. Int Braz J Urol 33:603-621.
Colagar AH, Jorsaraee GA, Marzony ET. 2007. Cigarette smoking and the risk of male infertility. Pak J Biol Sci 10:3870-3874.
Dandona P, Dhindsa S, Chaudhuri A, Bhatia V, Topiwala S, Mohanty P. 2008. Hypogonadotrophic hypogonadism in type 2 diabetes, obesity and the metabolic syndrome. Curr Mol Med 8:816-828.
Das SK, Ren R, Hashimoto T, Kanazawa K. 2010. Fucoxanthin induces apoptosis in osteoclast-like cells differentiated from RAW264.7 cells. J Agric Food Chem 58:6090-6095.
Dawson EB, Harris WA, Teter MC, Powell LC. 1992. Effect of ascorbic acid supplementation on the sperm quality of smokers. Fertil Steril 58:1034-1039.
Dembitsky VM, Maoka T. 2007. Allenic and cumulenic lipids. Prog Lipid Res 46:328-375.
Donath MY, Shoelson SE. 2011. Type 2 diabetes as an inflammatory disease. Nat Rev Immunol 11:98-107.
Donato J, Jr., Cravo RM, Frazao R, Elias CF. 2011. Hypothalamic sites of leptin action linking metabolism and reproduction. Neuroendocrinology 93:9-18.
Dong Q, Lazarus RM, Wong LS, Vellios M, Handelsman DJ. 1991. Pulsatile LH secretion in streptozotocin-induced diabetes in the rat. J Endocrinol 131:49-55.
Donnally CJ, 3rd, Gupta A, Bensalah K, Tuncel A, Raman J, Pearle MS, Lotan Y. 2011. Longitudinal evaluation of the SF-36 quality of life questionnaire in patients with kidney stones. Urol Res 39:141-146.
Donnelly ET, McClure N, Lewis SE. 2001. Cryopreservation of human semen and prepared sperm: effects on motility parameters and DNA integrity. Fertil Steril 76:892-900.
Ferruzzi MG, Blakeslee J. 2007. Digestion, absorption, and cancer preventative activity of dietary chlorophyll derivatives. Nutr Res 27:1-12.
Fouchecourt S, Metayer S, Locatelli A, Dacheux F, Dacheux JL. 2000. Stallion epididymal fluid proteome: Qualitative and quantitative characterization; Secretion and dynamic changes of major proteins. Biol Reprod 62:1790-1803.
George JT, Millar RP, Anderson RA. 2010. Hypothesis: kisspeptin mediates male hypogonadism in obesity and type 2 diabetes. Neuroendocrinology 91:302-307.
Geva E, Lessing JB, Lerner-Geva L, Amit A. 1998. Free radicals, antioxidants and human spermatozoa: clinical implications. Hum Reprod 13:1422-1424.
Henkel R. 2005. The impact of oxidants on sperm function. Andrologia 37:205-206.
Henkel R, Hajimohammad M, Stalf T, Hoogendijk C, Mehnert C, Menkveld R, Gips H, Schill WB, Kruger TF. 2004. Influence of deoxyribonucleic acid damage on fertilization and pregnancy. Fertil Steril 81:965-972.
Heo SJ, Jeon YJ. 2009. Protective effect of fucoxanthin isolated from Sargassum siliquastrum on UV-B induced cell damage. J Photochem Photobiol B 95:101-107.
Heo SJ, Kim JP, Jung WK, Lee NH, Kang HS, Jun EM, Park SH, Kang SM, Lee YJ, Park PJ, Jeon YJ. 2008a. Identification of chemical structure and free radical scavenging activity of diphlorethohydroxycarmalol isolated from a brown alga, Ishige okamurae. J Microbiol Biotechnol 18:676-681.
Heo SJ, Ko SC, Kang SM, Kang HS, Kim JP, Kim SH, Lee KW, Cho MG, Jeon YJ. 2008b. Cytoprotective effect of fucoxanthin isolated from brown algae Sargassum siliquastrum against H2O2-induced cell damage. Eur Food Res Technol 228:145-151.
Hofny ER, Ali ME, Abdel-Hafez HZ, Kamal Eel D, Mohamed EE, Abd El-Azeem HG, Mostafa T. 2010. Semen parameters and hormonal profile in obese fertile and infertile males. Fertil Steril 94:581-584.
Hosokawa M, Miyashita T, Nishikawa S, Emi S, Tsukui T, Beppu F, Okada T, Miyashita K. 2010. Fucoxanthin regulates adipocytokine mRNA expression in white adipose tissue of diabetic/obese KK-Ay mice. Arch Biochem Biophys 504:17-25.
Hughes CM, Lewis SE, McKelvey-Martin VJ, Thompson W. 1996. A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay. Mol Hum Reprod 2:613-619.
Ikeda K, Kitamura A, Machida H, Watanabe M, Negishi H, Hiraoka J, Nakano T. 2003. Effect of Undaria pinnatifida (Wakame) on the development of cerebrovascular diseases in stroke-prone spontaneously hypertensive rats. Clin Exp Pharmacol Physiol 30:44-48.
Irvine DS. 1998. Epidemiology and aetiology of male infertility. Hum Reprod 13 Suppl 1:33-44.
Jang KH, Lee BH, Choi BW, Lee HS, Shin J. 2005. Chromenes from the brown alga Sargassum siliquastrum. J Nat Prod 68:716-723.
Jarow JP, Espeland MA, Lipshultz LI. 1989. Evaluation of the azoospermic patient. J Urol 142:62-65.
Jarow JP, Sharlip ID, Belker AM, Lipshultz LI, Sigman M, Thomas AJ, Schlegel PN, Howards SS, Nehra A, Damewood MD, Overstreet JW, Sadovsky R. 2002. Best practice policies for male infertility. J Urol 167:2138-2144.
Jelodar G, Khaksar Z, Pourahmadi M. 2009. Endocrine profile and testicular histomorphometry in adult rat offspring of diabetic mothers. J Physiol Sci 59:377-382.
Kazlowska K, Hsu T, Hou CC, Yang WC, Tsai GJ. 2010. Anti-inflammatory properties of phenolic compounds and crude extract from Porphyra dentata. J Ethnopharmacol 128:123-130.
Kessopoulou E, Powers HJ, Sharma KK, Pearson MJ, Russell JM, Cooke ID, Barratt CLR. 1995. A Double-Blind Randomized Placebo Cross-over Controlled Trial Using the Antioxidant Vitamin-E to Treat Reactive Oxygen Species Associated Male-Infertility. Fertil Steril 64:825-831.
Keverne EB. 2007. Genomic Imprinting and the Evolution of Sex Differences in Mammalian Reproductive Strategies. Adv Genet 59:217-243.
Kim KN, Heo SJ, Yoon WJ, Kang SM, Ahn G, Yi TH, Jeon YJ. 2010. Fucoxanthin inhibits the inflammatory response by suppressing the activation of NF-kappa B and MAPKs in lipopolysaccharide-induced RAW 264 7 macrophages. European Journal of Pharmacology 649:369-375.
Konishi I, Hosokawa M, Sashima T, Kobayashi H, Miyashita K. 2006. Halocynthiaxanthin and fucoxanthinol isolated from Halocynthia roretzi induce apoptosis in human leukemia, breast and colon cancer cells. Comp Biochem Physiol C Toxicol Pharmacol 142:53-59.
Kotake-Nara E, Asai A, Nagao A. 2005a. Neoxanthin and fucoxanthin induce apoptosis in PC-3 human prostate cancer cells. Cancer Lett 220:75-84.
Kotake-Nara E, Terasaki M, Nagao A. 2005b. Characterization of apoptosis induced by fucoxanthin in human promyelocytic leukemia cells. Biosci Biotech Bioch 69:224-227.
La Vignera S, Calogero AE, Condorelli R, Lanzafame F, Giammusso B, Vicari E. 2009. Andrological characterization of the patient with diabetes mellitus. Minerva Endocrinol 34:1-9.
Laroux FS, Grisham MB. 2001. Immunological basis of inflammatory bowel disease: Role of the microcirculation. Microcirculation 8:283-301.
Le QT, Li Y, Qian ZJ, Kim MM, Kim SK. 2009. Inhibitory effects of polyphenols isolated from marine alga Ecklonia cava on histamine release. Process Biochem 44:168-176.
Lewis SEM, Aitken RJ. 2005. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res 322:33-41.
Liu CL, Liang AL, Hu ML. 2011. Protective effects of fucoxanthin against ferric nitrilotriacetate-induced oxidative stress in murine hepatic BNL CL.2 cells. Toxicol in Vitro 25:1314-1319.
Maeda H, Hosokawa M, Sashima T, Funayama K, Miyashita K. 2005a. Fucoxanthin from edible seaweed, Undaria pinnatifida, shows antiobesity effect through UCP1 expression in white adipose tissues. Biochem Biophys Res Commun 332:392-397.
Maeda H, Hosokawa M, Sashima T, Funayama K, Miyashita K. 2005b. Fucoxanthin from edible seaweed, Undaria pinnatifida, shows antiobesity effect through UCP1 expression in white adipose tissues. Biochem Bioph Res Co 332:392-397.
Maeda H, Hosokawa M, Sashima T, Miyashita K. 2007. Dietary combination of fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese/diabetic KK-Ay mice. J Agric Food Chem 55:7701-7706.
Maeda H, Hosokawa M, Sashima T, Murakami-Funayama K, Miyashita K. 2009. Anti-obesity and anti-diabetic effects of fucoxanthin on diet-induced obesity conditions in a murine model. Mol Med Report 2:897-902.
Maeda H, Tsukui T, Sashima T, Hosokawa M, Miyashita K. 2008. Seaweed carotenoid, fucoxanthin, as a multi-functional nutrient. Asia Pac J Clin Nutr 17 Suppl 1:196-199.
Mahan LK, Escott-Stump S, editors. 2010. Krause's Food, Nutrition, & Diet Therapy, 12 ed.
Martin SS, Qasim A, Reilly MP. 2008. Leptin resistance: a possible interface of inflammation and metabolism in obesity-related cardiovascular disease. J Am Coll Cardiol 52:1201-1210.
Martinez-Padilla J, Mougeot F, Webster LM, Perez-Rodriguez L, Piertney SB. 2010. Testing the interactive effects of testosterone and parasites on carotenoid-based ornamentation in a wild bird. J Evol Biol 23:902-913.
Masiello P, Broca C, Gross R, Roye M, Manteghetti M, Hillaire-Buys D, Novelli M, Ribes G. 1998. Experimental NIDDM: development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes 47:224-229.
Mei H, Walters C, Carter R, Colledge WH. 2011. Gpr54-/- mice show more pronounced defects in spermatogenesis than Kiss1-/- mice and improved spermatogenesis with age when exposed to dietary phytoestrogens. Reproduction 141:357-366.
Miyamoto T, Tsujimura A, Miyagawa Y, Koh E, Namiki M, Sengoku K. 2012. Male infertility and its causes in human. Adv Urol 2012:384520.
Miyashita K, Nishikawa S, Beppu F, Tsukui T, Abe M, Hosokawa M. 2011. The allenic carotenoid fucoxanthin, a novel marine nutraceutical from brown seaweeds. J Sci Food Agric 91:1166-1174.
Miyata M, Koyama T, Kamitani T, Toda T, Yazawa K. 2009. Anti-obesity effect on rodents of the traditional Japanese food, tororokombu, shaved Laminaria. Biosci Biotechnol Biochem 73:2326-2328.
Morris ID, Ilott S, Dixon L, Brison DR. 2002. The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development. Hum Reprod 17:990-998.
Mougeot F, Perez-Rodriguez L, Martinez-Padilla J, Leckie F, Redpath SM. 2007. Parasites, testosterone and honest carotenoid-based signalling of health. Funct Ecol 21:886-898.
Murphy KG, Curtis AE, Cooke JH, Baxter JE, Parkinson JRC, Bataveljic A, Ghatei MA, Bloom SR. 2010. A kisspeptin-10 analog with greater in vivo bioactivity than kisspeptin-10. Am J Physiol-Endoc M 298:E296-E303.
Naziroglu M, Butterworth PJ. 2005. Protective effects of moderate exercise with dietary vitamin C and E on blood antioxidative defense mechanism in rats with streptozotocin-induced diabetes. Can J Appl Physiol 30:172-185.
Nomura T, Kikuchi M, Kubodera A, Kawakami Y. 1997. Proton-donative antioxidant activity of fucoxanthin with 1,1-diphenyl-2-picrylhydrazyl (DPPH). Biochem Mol Biol Int 42:361-370.
Olayemi FO. 2010. A review on some causes of male infertility. Afr J Biotechnol 9:2834-2842.
Olefsky JM, Glass CK. 2010. Macrophages, Inflammation, and Insulin Resistance. Annu Rev Physiol 72:219-246.
Padron RS, Dambay A, Suarez R, Mas J. 1984. Semen analyses in adolescent diabetic patients. Acta Diabetol Lat 21:115-121.
Paolisso G, Gambardella A, Tagliamonte MR, Saccomanno F, Salvatore T, Gualdiero P, D'Onofrio MV, Howard BV. 1996. Does free fatty acid infusion impair insulin action also through an increase in oxidative stress? J Clin Endocrinol Metab 81:4244-4248.
Park HJ, Lee MK, Park YB, Shin YC, Choi MS. 2011. Beneficial effects of Undaria pinnatifida ethanol extract on diet-induced-insulin resistance in C57BL/6J mice. Food Chem Toxicol 49:727-733.
Pasqualotto FF, Lucon AM, Sobreiro BP, Pasqualotto EB, Arap S. 2004. Effects of medical therapy, alcohol, smoking, and endocrine disruptors on male infertility. Rev Hosp Clin Fac Med Sao Paulo 59:375-382.
Purvis K, Christiansen E. 1992. Male infertility: current concepts. Ann Med 24:259-272.
Rabbani SI, Devi K, Khanam S. 2010. Pioglitazone, a PPAR-gamma ligand inhibited the nicotinamide-streptozotocin induced sperm abnormalities in type-2 diabetic Wistar rats. Pak J Pharm Sci 23:326-331.
Rama Raju GA, Jaya Prakash G, Murali Krishna K, Madan K, Siva Narayana T, Ravi Krishna CH. 2012. Noninsulin-dependent diabetes mellitus: effects on sperm morphological and functional characteristics, nuclear DNA integrity and outcome of assisted reproductive technique. Andrologia 44 Suppl 1:490-498.
Ramadan SU, Yigit H, Gokharman D, Tuncbilek I, Dolgun NA, Kosar P, Kosar U. 2010. Can renal dimensions and the main renal artery diameter indicate the presence of an accessory renal artery? A 64-slice CT study. Diagn Interv Radiol.
Reddi AS, Bollineni JS. 1997. Renal cortical expression of mRNAs for antioxidant enzymes in normal and diabetic rats. Biochem Biophys Res Commun 235:598-601.
Sachindra NM, Sato E, Maeda H, Hosokawa M, Niwano Y, Kohno M, Miyashita K. 2007a. Radical scavenging and singlet oxygen quenching activity of marine carotenoid fucoxanthin and its metabolites. J Agric Food Chem 55:8516-8522.
Sachindra NM, Sato E, Maeda H, Hosokawa M, Niwano Y, Kohno M, Miyashita K. 2007b. Radical scavenging and singlet oxygen quenching activity of marine carotenoid fucoxanthin and its metabolites. J Agr Food Chem 55:8516-8522.
Salguero A, de la Morena B, Vigara J, Vega JM, Vilchez C, Leon R. 2003. Carotenoids as protective response against oxidative damage in Dunaliella bardawil. Biomolecular Engineering 20:249-253.
Samaha FF, Szapary PO, Iqbal N, Williams MM, Bloedon LT, Kochar A, Wolfe ML, Rader DJ. 2006. Effects of rosiglitazone on lipids, adipokines, and inflammatory markers in nondiabetic patients with low high-density lipoprotein cholesterol and metabolic syndrome. Arterioscler Thromb Vasc Biol 26:624-630.
Scarano WR, Messias AG, Oliva SU, Klinefelter GR, Kempinas WG. 2006. Sexual behaviour, sperm quantity and quality after short-term streptozotocin-induced hyperglycaemia in rats. Int J Androl 29:482-488.
Seethalakshmi L, Menon M, Diamond D. 1987. The effect of streptozotocin-induced diabetes on the neuroendocrine-male reproductive tract axis of the adult rat. J Urol 138:190-194.
Sexton WJ, Jarow JP. 1997. Effect of diabetes mellitus upon male reproductive function. Urology 49:508-513.
Sharlip ID, Jarow JP, Belker AM, Lipshultz LI, Sigman M, Thomas AJ, Schlegel PN, Howards SS, Nehra A, Damewood MD, Overstreet JW, Sadovsky R. 2002. Best practice policies for male infertility. Fertil Steril 77:873-882.
Sharma RK, Agarwal A. 1996. Role of reactive oxygen species in male infertility. Urology 48:835-850.
Shimoda H, Tanaka J, Shan SJ, Maoka T. 2010. Anti-pigmentary activity of fucoxanthin and its influence on skin mRNA expression of melanogenic molecules. J Pharm Pharmacol 62:1137-1145.
Sierens J, Hartley JA, Campbell MJ, Leathem AJ, Woodside JV. 2002. In vitro isoflavone supplementation reduces hydrogen peroxide-induced DNA damage in sperm. Teratog Carcinog Mutagen 22:227-234.
Sikka SC. 2004. Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. J Androl 25:5-18.
Singh R, Barden A, Mori T, Beilin L. 2001. Advanced glycation end-products: a review. Diabetologia 44:129-146.
Singh S, Loke YK, Furberg CD. 2007. Long-term risk of cardiovascular events with rosiglitazone: a meta-analysis. JAMA 298:1189-1195.
Skakkebaek NE, O'Shaughnessy PJ, Page D, Sharpe R, Ivell R. 2006. The foetal Leydig cell - differentiation, function and regulation - Discussion. Int J Androl 29:95-95.
Smith JT, Acohido BV, Clifton DK, Steiner RA. 2006. KiSS-1 neurones are direct targets for leptin in the ob/ob mouse. J Neuroendocrinol 18:298-303.
Steger RW, Rabe MB. 1997. The effect of diabetes mellitus on endocrine and reproductive function. Proc Soc Exp Biol Med 214:1-11.
Sugawara T, Matsubara K, Akagi R, Mori M, Hirata T. 2006. Antiangiogenic activity of brown algae fucoxanthin and its deacetylated product, fucoxanthinol. J Agric Food Chem 54:9805-9810.
Tena-Sempere M. 2009. [Neuroendocrinology of reproduction: the kisspeptin age]. Endocrinol Nutr 56:103-105.
Tsukui T, Baba N, Hosokawa M, Sashima T, Miyashita K. 2009. Enhancement of hepatic docosahexaenoic acid and arachidonic acid contents in C57BL/6J mice by dietary fucoxanthin. Fisheries Sci 75:261-263.
van Melle JP, Bot M, de Jonge P, de Boer RA, van Veldhuisen DJ, Whooley MA. 2010. Diabetes, glycemic control, and new-onset heart failure in patients with stable coronary artery disease: data from the heart and soul study. Diabetes Care 33:2084-2089.
Vernet P, Aitken RJ, Drevet JR. 2004. Antioxidant strategies in the epididymis. Molecular and Cellular Endocrinology 216:31-39.
Vlassara H, Palace MR. 2002. Diabetes and advanced glycation endproducts. J Intern Med 251:87-101.
Wautier JL, Schmidt AM. 2004. Protein glycation - A firm link to endothelial cell dysfunction. Circulation Research 95:233-238.
Wells-Knecht MC, Lyons TJ, McCance DR, Thorpe SR, Baynes JW. 1997. Age-dependent increase in ortho-tyrosine and methionine sulfoxide in human skin collagen is not accelerated in diabetes. Evidence against a generalized increase in oxidative stress in diabetes. J Clin Invest 100:839-846.
WHO. 2000. WHO Manual for the Standardised Investigation and Diagnosis of the Infertile Couple. In: University C, editor.
WHO. 2011. Diabetes fact sheet. In: http://www.who.int/mediacentre/factsheets/fs312/en/index.html.
Wong WY, Thomas CMG, Merkus JMWM, Zielhuis GA, Steegers-Theunissen RPM. 2000. Male factor subfertility: possible causes and the impact of nutritional factors. Fertil Steril 73:435-442.
Woo MN, Jeon SM, Kim HJ, Lee MK, Shin SK, Shin YC, Park YB, Choi MS. 2010. Fucoxanthin supplementation improves plasma and hepatic lipid metabolism and blood glucose concentration in high-fat fed C57BL/6N mice. Chem Biol Interact 186:316-322.
Woo MN, Jeon SM, Shin YC, Lee MK, Kang MA, Choi MS. 2009. Anti-obese property of fucoxanthin is partly mediated by altering lipid-regulating enzymes and uncoupling proteins of visceral adipose tissue in mice. Mol Nutr Food Res 53:1603-1611.
Yan XJ, Chuda Y, Suzuki M, Nagata T. 1999. Fucoxanthin as the major antioxidant in Hijikia fusiformis, a common edible seaweed. Biosci Biotech Bioch 63:605-607.
Yang SK, Attipoe S, Klausner AP, Tian R, Pan D, Rich TA, Turner TT, Steers WD, Lysiak JJ. 2006. In vivo detection of apoptotic cells in the testis using fluorescence labeled annexin V in a mouse model of testicular torsion. J Urol 176:830-835.
Zaragoza MC, Lopez D, Saiz MP, Poquet M, Perez J, Puig-Parellada P, Marmol F, Simonetti P, Gardana C, Lerat Y, Burtin P, Inisan C, Rousseau I, Besnard M, Mitjavila MT. 2008. Toxicity and antioxidant activity in vitro and in vivo of two Fucus vesiculosus extracts. J Agr Food Chem 56:7773-7780.
Zhang ZB, Jiang YT, Yun X, Yang X, Wang RX, Dai RL, Liu RZ. 2012. Male infertility in Northeast China: a cytogenetic study of 135 patients with non-obstructive azoospermia and severe oligozoospermia. J Assist Reprod Genet 29:83-87.

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