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研究生:羅葳娜
研究生(外文):Rowena King
論文名稱:估算台灣綠蠵龜稚龜性別比之研究
論文名稱(外文):Estimating the Sex Ratio of Green Sea Turtle (Chelonia mydas) Hatchlings in Taiwan
指導教授:程一駿程一駿引用關係
指導教授(外文):I-Jiunn Cheng
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:海洋生物研究所
學門:自然科學學門
學類:海洋科學學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:英文
論文頁數:53
中文關鍵詞:Chelonia mydas海龜性别比臺灣組織切片卵窩溫度
外文關鍵詞:Chelonia mydassea turtle sex ratioTaiwanhistology methodnest temperature
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  性別比是族群生活史中重要的特性之一,海龜的性别是由孵化過程中的卵窩溫度所決定,但是,我們很難去驗證海龜的性別比。洄游的成熟海龜並不是每年固定回到產卵沙灘產卵,大洋中的生活的幼龜也很難去得知其性别比,但是已死亡的稚龜卻是驗證海龜族群性别比的最佳替代品。了解海龜性别比方法主要有,直接觀察海龜性線的組織切片法與利用卵窩溫度來評估的間接推測法。在本次實驗中,我們第一次結合兩種研究方式,來了解台灣地區綠蠵龜族群的性別比。於2010及2011的產卵季中,我們收集了蘭嶼、望安及小琉球等三個主要產卵沙灘的死亡稚龜進行實驗。結果顯示,望安及小琉球分別有91.2%與100%的個體為雌性,而蘭嶼則為較低的75%個體為雌性,所以蘭嶼孵化出較多的雄性稚龜。儘管蘭嶼有較高百分比的雄性稚龜,但本實驗結果還是顯示出,臺灣地區綠蠵龜族群的性別偏雌性居多。

In sea turtles, sex is determined by the temperature during the incubation. Sex ratio is one of the important life history traits of the population. To determine the sex ratio of sea turtle, however, has proven difficult. Adults are migratory and do not return to their nesting beaches every year and juvenile sex ratios are equally difficult to determine. Using dead hatchlings can provide us with the best alternative sex ratio estimates of that population. Histology is described as a direct method to determine the sex ratio of hatchlings and indirect method including the recording of nest incubation temperature. Both methods are used in this study to determine, for the first time, the sex ratio of green turtle (Chelonia mydas) population in Taiwan. Dead hatchlings were collected from three major nesting areas (Lanyu, Wan-an and Xiao Liuqiu) in the 2010 and 2011 nesting seasons. Results showed that Wan-an and Xiao Liuqiu produced 91.2% and 100% females respectively and Lanyu produced a lower sex ratio of 75% females. Lanyu has a higher probability of producing male hatchlings. In spite of the higher percentage of male hatchling produced from that island, results from this study showed a female-biased sex ratio of the green turtle population in Taiwan
Table of Contents
Abstract i
摘要 ii
Table of Contents iii
List of Figures vi
List of Tables viii
General Introduction 1
Introduction 4
Green turtles taxonomy and description 4
Life history/cycle 5
Threats in Taiwan 5
Temperature Sex determination 6
Methods used to predict sex ratio 8
Purpose of the study 10
Materials and Methods 11
Study Site 11
Field work 12
Nesting beach surveys: 12
Sand temperature recording: 12
Laboratory work 13
Sex determination of the hatchlings in the laboratory 13
Tissue processing: 13
Sample embedding: 13
Sectioning: 14
Staining: 14
Sex determination: 16
Data analysis 17
Results 18
Number of nests and dead hatchling sampled from each island during the study period 18
Sex ratio estimated from histology of hatchlings 18
Variation in nest temperature during the sex determination period 19
Estimated nest temperatures from sand temperature on Wan-an Island 20
Nest temperature and sex ratio data for nesting beaches in Taiwan 20
Discussion 22
Number of nests and dead hatchling sampled from each island during the study period 22
Sex ratio estimated from histology of hatchlings 22
Variation in nest temperature during the sex determination period 25
Estimated nest temperatures from sand temperature for Wan-an Island in 2010 26
Nest temperature and sex ratio data for nesting beaches in Taiwan 27
Recommendations 28
References 29
Figures 36
Tables 50




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