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研究生:洪健文
論文名稱:矽藻二次代謝物poly unsaturated aldehyde對橈足類Acartia bilobata游泳行為之影響
論文名稱(外文):Effect of the secondary metabolites, poly unsaturated aldehyde on the swimming behavior of Acartia bilobata
指導教授:黃將修
指導教授(外文):Hwang Jiang-Shiou
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:海洋生物研究所
學門:自然科學學門
學類:海洋科學學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:63
中文關鍵詞:矽藻撓足類游泳行為
外文關鍵詞:diatomcopepod swimming behavior
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  • 被引用被引用:2
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本研究是針對橈足類Acartia bilobata的成熟雌蟲在添加矽藻所產生不同的三種二次代謝物poly-unsaturated aldehyde(decadienal, heptadienal and octadienal)條件環境下,記錄並比較其游泳行為上的差異,行為軌跡利用手動追蹤影片中饒足類游泳的方式建立,我們對於游泳行為的分析,主要著重在平均游泳速度、跳動頻率以及碎行維度(NGDR)上,,其目的是為得知在受到PUAs刺激時橈足類除了對於子代生產及發育造成影響外,對於成蟲是否也會有生理或行為上的影響,研究結果顯示Acartia bilobata的游泳行為的數據統計分析結果在不同濃度環境下是有顯著差異存在,但與控制組之間卻沒有差異,這表示存在PUA的環境確實對Acartia bilobata的行為有影響,因為PUA不屬於急性刺激因此環境造成的刺激導致橈足類出現逃避行為但是並不明顯。
目錄

謝辭 i
中文摘要 ii
英文摘要 iii
目錄......................................................v
表目錄....................................................vi
圖目錄 viii
壹、前言 1
一、 海洋生態系的營養階層 1
二、 橈足類在海洋生態系的角色 2
三、 橈足類的食性 3
四、 橈足類與浮游矽藻的交互作用 5
五、 實驗動機與目的 7
貳、材料與方法 8
1.橈足類大量培養及蒐集 8
2.PUA對橈足類的急毒性測試 8
3.光學設備的架設與錄製 9
4.影像的剪輯與行為軌跡的數據化 11
5.橈足類游泳行為之觀察與紀錄 11
6.數據計算與分析 12
7.淨-移動位移比NGDR(net to gross displacement ratio).....13
8.致死濃度與信賴區間之估計 14
9.統計分析 14
參、結果 15
1、急毒性測試 15
2、橈足類Acartia bilobata之游泳行為描述 17
3、橈足類Acartia bilobata雌性成蟲NGDR分析 24
4、橈足類Acartia bilobata雌性成蟲平均游泳速度分布及統計分析 27
5、橈足類Acartia bilobata雌性成蟲平均跳動頻率統計分析 28
肆、討論 30
一、PUA對於橈足類Acartia bilobata成蟲急毒性測試 30
二、PUA對於橈足類Acartia bilobata雌性成蟲之游泳行為影響 31
伍、參考文獻 36
陸、表...................................................43
柒、圖...................................................52

表目錄

表次.............................. ....................頁數
表1、橈足類Acartia bilobata成蟲在不同PUA環境下的致死濃度與LC50信賴區間.................................................43
表2、橈足類Acartia bilobata雌性成蟲在Heptadienal不同濃度環境下的游泳數據.............................................44
表3、橈足類Acartia bilobata雌性成蟲在Octadienal不同濃度環境下的游泳數據.............................................45
表4、橈足類Acartia bilobata雌性成蟲在Decadienal不同濃度環境下的游泳數據.............................................46
表5、橈足類Acartia bilobata雌性成蟲在三種PUAs下的NGDR分析.......................................................47
表6、橈足類Acartia bilobata雌性成蟲平均游泳速度與NGDR值在變因為三種PUA時之統計分析.................................48
表7、橈足類Acartia bilobata雌性成蟲平均游泳速度與NGDR值在變因為四種濃度環境時之統計分析.............................49
表8、橈足類Acartia bilobata雌性成蟲跳動頻率在變因為四種濃度環境時之統計分析...........................................50
表9、橈足類Acartia bilobata雌性成蟲跳動頻率在變因為三種PUA時之統計分析...............................................51

















圖目錄

表次.............................. ....................頁數
圖1、非線性剪輯軟體Adobe Premiere Pro 2.0之操作介面....52
圖2、手動追蹤軟體Trackit之操作介面圖...................53
圖3、橈足類Acartia bilobata成蟲在Heptadienal之LC50曲線.......................................................54
圖4、橈足類Acartia bilobata成蟲在Octadienal之LC50測試曲線.......................................................55
圖5、橈足類Acartia bilobata成蟲在Decadienal之LC50測試曲線圖.......................................................56
圖6、控制組橈足類Acartia bilobata雌性成蟲軌跡圖........57
圖7、橈足類Acartia bilobata雌性成蟲在Heptadienal環境軌跡圖.......................................................58                               
圖8、橈足類Acartia bilobata雌性成蟲在Octadienal環境軌跡圖.......................................................59
圖9、橈足類Acartia bilobata雌性成蟲在Decadienal環境軌跡圖.......................................................60
圖10、各組橈足類Acartia bilobata雌性成蟲跳動速度分布圖.......................................................61
圖11、各組橈足類Acartia bilobata雌性成蟲NGDR值分布圖.......................................................62
圖12、各組橈足類Acartia bilobata雌性成蟲跳動頻率分布圖.......................................................63



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