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研究生:劉冠儀
研究生(外文):Kuan-Yi Liu
論文名稱:光照時數對聚球藻WH7803藻紅蛋白含量之影響
論文名稱(外文):The light effect on the production of phycoerythrin in Synechoccus sp. WH7803
指導教授:熊同銘許邦弘許邦弘引用關係
指導教授(外文):Tung-Ming HsiungPang-Hung Hsu
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:78
中文關鍵詞:藻紅蛋白代謝物同位素標定層析串連式質譜聚球藻
外文關鍵詞:phycoerythrinSILACLC-MS/MSSynechococcus sp
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本研究以聚球藻(Synechococcus sp. WH7803)作為藍綠藻的代表,以光:暗比6:18及24:0分別培養兩組聚球藻,藉以探討環境因子例如光照時數對藍綠藻之中藻紅蛋白的含量之影響。每組中另分別以14N及15N作為生長環境中之氮源區分為兩個次組,1週後取同數目之細胞混合研磨萃取,經由蛋白質電泳純化後加入Trypsin將其蛋白質水解成小分子的胜肽,再利用LC-MS/MS 分析。檢測數據採用代謝物同位素標定法(SILAC),除了可進行藻紅蛋白鑑定外,亦可一併完成光照長短對於聚球藻WH7803內藻紅蛋白差異量比較。
由實驗結果可知在短光照時數下所產生之藻紅蛋白含量會較長光照時數較多,含量約為4-5倍。在光照不足的情況下,藻紅蛋白會大量表現以獲得足夠的能量來維持藻類的生理機能。
This research focused on Synechococcus sp. WH7803 which is one of the members of the Cyanophyta genus. The Synechococcus sp. WH7803 was cultivated in two different groups with a light and dark ratio 6:18 and 24:0. Each group was separated into two sub-groups by 14N and 15N environments. The same number from per group of cell was extracted and purified by electrophoresis (SDS-PAGE). Trypsin was added for digesting the protein into peptide for the LC-MS/MS analysis. The data were adopted by SILAC for phycoerythrin identification and quantification of phycoerythrin under various exposure time.
The result showed the phycoerythrin in WH7803 was 4 to 5 times richer in short exposure time. In case of insufficient light, the more expression of phycoerythrin in order to obtain sufficient energy to maintain the physiology of Synechococcus sp.

謝誌 I
摘要 II
英文摘要 III
目錄 IV
圖目錄 VI
表目錄 VII
附錄目錄 VIII
第一章 前言 1
1.1 聚球藻 1
1.2 藻膽蛋白及藻紅蛋白 1
1.3 蛋白質測定方法 2
1.4 代謝物同位素標定 3
1.5 研究動機與目的 4
第二章 設備與方法 5
2.1 儀器設備 5
2.2 試藥 5
2.3 藻類培養 7
2.4 藻類萃取 9
2.5 膠體電泳 9
2.6 膠體內水解 11
2.7 上機處理 13
第三章 結果與討論 14
3.1 聚球藻之培養 14
3.2 藻膽蛋白的鑑定 15
3.3 光照時數造成之影響 18
第四章 結論 20
參考文獻 22

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