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研究生:尤妮
研究生(外文):Yuni Paramitha Sari
論文名稱:從楝樹葉中分離及定性抗氧化劑化合物
論文名稱(外文):Isolation and Characterization of Antioxidant Compounds From Azadirachta indica Leaves
指導教授:朱義旭
指導教授(外文):Yi-Hsu Ju
口試委員:朱義旭
口試日期:2012-07-03
學位類別:碩士
校院名稱:國立臺灣科技大學
系所名稱:化學工程系
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:英文
論文頁數:52
外文關鍵詞:antioxidantisolationneemazadirachta indica
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天然抗氧化劑可以保護人體免受自由基傷害並減緩許多慢性疾病惡化。楝樹在印度是一種廣為人知的多用途藥用植物且具有廣泛的生物活性。
此研究主要的目的為從印尼種楝樹的葉子中分離及定性抗氧化劑化合物。乾燥的楝樹葉在搖床上用80%(v/v)的甲醇在30°C萃取24小時。接著萃取物進行過濾和用迴轉蒸發濃縮機濃縮。以水溶解乾燥的萃取物後,使用己烷、乙酸乙酯和正丁醇使其分相。乙酸乙酯相有最高的抗氧化性,被用來做進一步的純化和測定。使用矽膠管柱層析法進行酚類化合物的分離和純化,薄層層析法矽膠60 GF254被用來決定最佳的展開劑。使用高效能液相層析儀和紫外光/可見光光譜儀對純化後的化合物進行測定。
高效能液相層析儀測定的結果顯示,四種次要的化合物為阿魏酸、楊梅黃素、山奈酚和槲皮素。經由紫外光/可見光光譜儀測定的結果顯示,木犀草素-7-O-β-D-葡萄糖苷為主要化合物的其中一種。
Natural antioxidants can protect human body from free radicals and retard the progress of many chronic diseases. Neem (Azadirachta indica A. Juss) is well known in India as one of the most versatile medicinal plants having a wide spectrum of biological activity.
The aim of this study was to isolate and characterize antioxidant compounds from leaf of neem of species of Indonesia. The dried neem leaves were extracted using 80% (v/v) methanol at 30°C for 24 h on a shaker. The extract was filtered and concentrated in vacuo in a rotary evaporator. The dried extract was dissolved in water and partitioned with hexane, ethyl acetate, and butanol. Ethyl acetate fraction showed the highest antioxidant activity and was subjected to further purification and identification. Isolation and purification of phenolic compounds were done using silica gel column chromatography. Elution solvent was selected using TLC silica gel 60 GF254. The purified compounds were identified using HPLC and UV-Vis spectroscopy.
The four minor compounds identified by HPLC are ferulic acid, myricetin, kaempferol and quercetin. One of the major compounds was identified using UV-Vis spectroscopy as luteolin-7-O-β-D-glucopyranoside.
TABLE OF CONTENTS

CHINESE ABSTRACT i
ENGLISH ABSTRACT ii
ACKNOWLEDGEMENT iii
TABLE OF CONTENTS iv
LIST OF FIGURES vi
LIST OF TABLES vii
CHAPTER 1 INTRODUCTION 1
CHAPTER 2 LITERATURE REVIEW 3
2.1. Azadirachta indica 3
2.2. Antioxidant 8
2.3. Phenolic Compounds 9
2.4. Extraction of Phenolic Compounds 13
2.5. Preparative Separation 14
CHAPTER 3 MATERIALS AND METHODS 18
3.1. Materials 18
3.2. Equipments 18
3.3. Methodology 19
3.3.1. Extraction Procedure 19
3.3.2. Solvent Partitioning of Crude Extract 20
3.3.3. TLC Screening for Antioxidant Compounds 20
3.3.4. Column Chromatography 21
3.4. Analysis 21
3.4.1. Analysis by HPLC 21
3.4.2. Analysis by UV-Vis Spectroscopy 22
CHAPTER 4 RESULTS AND DISCUSSION 23
4.1. Extraction and Separation 23
4.2. Isolation of Compounds 25
4.3. Major Compounds 31
CHAPTER 5 CONCLUSION 33
REFERENCES 34
AUTOBIOGRAPHY 42
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