跳到主要內容

臺灣博碩士論文加值系統

(3.235.56.11) 您好!臺灣時間:2021/07/29 10:02
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:陳群諭
研究生(外文):Chun-Yu Chen
論文名稱:蚌蘭全草之化學成分研究
論文名稱(外文):Phytochemical study on Rhoeo discolor
指導教授:黎世源黎世源引用關係黃鈺玲黃鈺玲引用關係
指導教授(外文):Shyh-Yuan LiYu-Ling Huang
口試委員:沈建昌郭文貴
口試日期:2012-06-27
學位類別:碩士
校院名稱:中國文化大學
系所名稱:化學系應用化學碩士班
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:128
中文關鍵詞:蚌蘭鴨跖草科呋喃酮抗發炎抗癌
外文關鍵詞:Rhoeo discolorcommelinaceaefuranoneanti-inflammatoryanti-cancer
相關次數:
  • 被引用被引用:1
  • 點閱點閱:264
  • 評分評分:
  • 下載下載:5
  • 收藏至我的研究室書目清單書目收藏:0
蚌蘭(Rhoeo discolor (L'Her.) Hance)屬於鴨跖草科(Commelinaceae)蚌蘭屬(Rhoeo),又名紫背鴨跖草、紫背萬年青、水紅竹、紅三七。植物全草主用於治消炎、止瀉、瘀血、肺炎、咳嗽、肝病等症。本研究主要探討蚌蘭之化學成分,及其抗發炎以及抗癌活性。風乾後之植物全草,以95%乙醇於55 ℃下抽取,所得之乙醇抽取物,依序溶解並分為CH2Cl2-Me2CO (1:1)可溶與MeOH可溶兩部分,各部分利用管柱色層層析法分離,純化共得到九個化合物,藉由核磁共振光譜資料分析與質譜之佐證確立化合物之構造,其中化合物 1, 2, 3 屬於新化合物,化合物 4, 6 則為首次由自然界分離得到,各部分所得化合物分別為(R)-3-(3,4-dihydroxyphenyl)-5-hydroxy-5H-furan-2-one (1)、 (R)-3-(3,4-dihydroxyphenyl)-5-methoxy-5H-furan-2-one (2)、 3-(3,4-dihydroxyphenyl)-5H-furan-2-one (3)、4-((E)-3-(3,4-dihydroxyphenyl)acryloyloxy)-2,3-dihydroxy-2-methylbutanoic acid (4)、(E)-ferulic acid hexacosyl ester (5)、quercetin-3-O-β-D-glucoside (6''→1''')- α-L-arabinoside (6)、L-adenosine (7)、3,4-dihydroxybenzoic acid (8) 和 compound 9。生物活性試驗結果顯示,蚌蘭之莖、花及葉粗抽物之CH2Cl2-Me2CO (1:1)可溶部分在濃度1、10、50及100 μg/mL的下呈現劑量相關性的抑制RAW 267.7細胞株由LPS所誘導的NO過量生成,其EC50分別為51.81、46.85、85.48 μg/mL,而MTT分析的TC50值均大於1000 μg/mL;抗癌活性部分, 蚌蘭之莖、花、葉粗抽物與化合物1、2、6針對於MCF-7 (乳癌)、NCl-H460 (肺癌)、HT-29 (大腸癌)、CEM (淋巴癌)細胞系進行試驗,結果顯示均無明顯抑制效果。
Rhoeo discolor (L'Her.) Hance (Commelinaceae) is a well-know folk medicine in taiwan. The whole herbs of this plant are used to treat in flammation, bruising, pneumonia, cough and liver fibrosis, etc. In this study, the chemical constituents of the whole plant of R.discolor were investigated and their anti-inflammatory and anti-cancer activities were tested. Air-dried studied material was extracted with 95% EtOH at 55℃ for 24 hr. After evaporation of the solvent in vaco, the extract was trented with CH2Cl2-Me2CO (1:1) and then MeOH to obtain two soluble portions. They were individually and purified by a combination of several chromatographic techniques, including led to the isolation of nine compounds which were (R)-3-(3,4-dihydroxyphenyl)-5-hydroxy-5H-furan-2-one (1), (R)-3-(3,4-dihydroxyphenyl)-5-methoxy-5H-furan-2-one (2), 3-(3',4'-dihydroxyphenyl)-5H-furan-2-one (3), 4-((E)-3-(3,4-dihydroxyphenyl)acryloyloxy)-2,3-dihydroxy-2-methylbutanoic acid (4), (E)-ferulic acid hexacosyl ester(5), quercetin-3-O-β-D-glucoside(6''→1''') -α-L-arabinoside (6), L-adenosine (7), 3,4-dihydroxybenzoic acid (8) and compound 9. The structures of isolated compounds were established by application of spectroscopic analyses (NMR, MS, UV, IR). Among these compounds, 1, 2 and 3 are new compounds, as well as 4 and 6 are first derived from natural source.
The preliminary results of showed that the CH2Cl2-Me2CO(1:1) soluble portion extracts of the stem, flower and leaf of R.discolor displayed dose-dependent anti-inflammatory effect at 1, 10, 50 and 100μg/mL on lipopolysaccaride(LPS)-induced nitric oxide (NO) production in RAW 267.7 macrophages. Their EC50 values were 51.81, 46.85, 85.48 μg/mL, respectively. The TC50 values of MTT assay were greater than 1000 μg / mL. The above three extracts and compounds 1, 2, and 6 showed no significant anti-cancer activities on MCF-7 (breast), NCl-H460 (lung), HT-29(colon) and CEM (leukemia) cancer cells.
中文摘要--------------------------------------------------------------------------- I
英文摘要------------------------------------------------------------------------- III
目次-------------------------------------------------------------------------------- V
圖表目次------------------------------------------------------------------------- VI
前言-------------------------------------------------------------------------------- 1
實驗部分-------------------------------------------------------------------------- 4
結果與討論---------------------------------------------------------------------- 10活性試驗部分------------------------------------------------------------------- 34
結論------------------------------------------------------------------------------- 35
相關成分物理性質------------------------------------------------------------- 37
參考文獻------------------------------------------------------------------------- 46
附錄------------------------------------------------------------------------------- 48
國家中醫藥管理局編委會, 2004, 中華草本(8), 上海科學技術出版社, pp. 309-310.
江蘇新醫學院, 1977, 中藥大辭典(下), 上海科學技術出版社, pp. 850-851.
邱年永, 張光雄, 1992, 原色台灣藥用植物圖鑑(2), 台北南天書局, pp. 268.
台北台灣植物誌第二版編委會, 1998, 台灣植物誌第二版(4), 台北台灣植物誌第二版編委會, pp. 153-178.
中國科學院中國植物誌編委會, 1979. 中國植物誌 75, 科學出版社, pp. 24-26.
黃麗莎, 朱峰, 2009, 蚌蘭花揮發油化學成分的GC-MS分析, 中藥材. 32(1), 65-66.
童熯民, 蔡清恩, 2009, 蚌蘭萃取物的藥理活性與其沒食子酸成分含量分析之研究, 國立屏東科技大學碩士論文, pp. 1-2.
Duke, C. C.; Letham, D. S.; Parker, C. W.; Macleodt, J. K.; Summons, R. E., The Complex of O-glucosylzeatin derivattves formed in Populus Species. Phytochemistry 1979, 18, 819-824.
Faber, W. S.; Kok, J.; Lange B. D.; Feringa, B. L., Catalytic kinetic resolution of 5-alkoxy-2(5H)-furanones. Tetrahedron 1994, 50, 4775-4794.
Gonza´lez-A´ vila, M.; Arriaga-Alba, M.; De la Garza, M.; Herna´ndez -
Pretel´ın, M.C.; Dom´ınguez-Ort´ız, M.A.; Fattel-Fazenda, S.; Villa-
Trevi˜no, S., Antigenotoxic, antimutagenic and ROS scavenging activities of a Rhoeo discolor ethanolic crude extract, Toxicology in Vitro 2003, 17, 77-83.
Hui, L. Z.; Akito, N.; Harumi, O.; Hajime, M.; Jinsaku, S., Sesquiterpene glycosides from cotton oil cake. Phytochemistry 1998, 48, 665-668.
Ivan, A.; Hans, A.; Grace, N. T.; Reiner, W.; Julius, W. M., Epoxychiromodine and other constituents of Croton megalocarpus. Phytochemistry 1992, 31, 2055-2058.
Itoh, A.; Tanahashi, T.; Nagakura, N., Two Phenolic Glucosides and an iridoid glucoside from alangium plangium platnifolium var. trilobum. Phytochemistry 1993, 33, 161-164.
Lin, L. C.; Chou, C. J.; Yang, L. M., Chemical constituents from the fruit of Spiraea formosana. Chin. Pharm. J. 1999, 51, 299-305.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊