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研究生:許瑋真
研究生(外文):Hsu, Weichen
論文名稱:茄冬花部抗氧化活性成分研究 (II)
論文名稱(外文):Studies On The Antioxidant Activity Constituents Of Bischofia Javanica Flower (II)
指導教授:黃克峰黃克峰引用關係
指導教授(外文):Huang, Kehfeng
口試委員:劉美珍鐘婷婷
口試委員(外文):Liou, MeeijenZhong, Tingting
口試日期:2012-06-27
學位類別:碩士
校院名稱:靜宜大學
系所名稱:應用化學系
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:124
中文關鍵詞:茄冬花部
外文關鍵詞:Bischofia javanica flower
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  • 下載下載:8
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茄冬樹,又名重陽木,屬於大戟科(Euphorbiaceae)植物,學名 Bischofia jabanica Blume.,半落葉喬木,廣泛被栽植為行道樹,且具有驅蟲、抗痢疾及抗潰瘍等功效。
本研究以茄冬雌花部80%酒精水溶液初抽物進行總黃酮類化合物含量、總多酚類化合物含量以及DPPH自由基清除能力評估,發現乙酸乙酯層及正丁醇層擁有較高活性,其清除自由基能力為 EC50=76.4μg/mL (乙酸乙酯層) 及EC50=70.46μg/mL (正丁醇層),總黃酮類化合物含量分別為514.87 mg/g flavonoid (乙酸乙酯層)及318.30 mg/g flavonoid (正丁醇層),總多酚類化合物含量分別為965.30 mg/g gallic acid (乙酸乙酯層)及640.03 mg/g gallic acid (正丁醇層),此二可溶層繼續經管柱層析以活性為導向分離得到 gallic acid、isocoriligen(屬於gallic acid衍生物) 以及quercetin-3-O-rutinoside和naringenin等兩種黃酮類化合物,均經過光譜分析確定其結構。
Bischofia javanica Blume., Euphorbiaceae, is a cultivated species in Taiwan as a roadside tree, and had the anti-ulcer, the expelling worms and anti-dysenteric activites.
The antioxidant activity of 80% ethanol extract and its subfractions of Bischofia javanica flower, was evaluated using the DPPH free radical scavenging ability.The total phenolic content of theses extracts was determined as gallic acid equivalent. The total flavonoids content of theses extracts was determined as quercetin equivalent.
The ethyl acetate soluble fraction and n-butanol soluble fraction of Bischofia javanica flower possessed the higher DPPH free radical scavenging, (EC50=70.40 μg/mL and 70.46 μg/mL, respectively). The total phenolic content were estimated as 965.30 mg/g gallic acid and 640.03 mg/g gallic acid, respectively. The total flavonoids content were estimated as 514.87 mg/g flavonoids and 318.30 mg/g flavonoids, respectively.
The active components were obtained from column chromatography, namedly, gallic acid, isocorilagin, quercetin-3-O-rutinoside, and naringenin.
The structures were elucidated by spectroscopic methods and comparison with an authentic sample.
第一章 緒論 4
第一節 植物介紹 4
第二節 研究動機 7
第三節 茄冬樹成分之相關文獻報導 8
第四節 文獻記載有關茄冬樹之結構 10
第五節 抗氧化活性物質介紹 17
一、活性氧物質與自由基介紹 17
二、活性氧對生物體之傷害性探討 23
三、生物體之抗氧化防禦系統 30
四、天然抗氧化劑的活性成分介紹 35
五、抗氧化活性篩選方法 42
第二章 實驗部分 44
第一節 儀器 44
第二節 試藥與溶劑 45
第三節 實驗方法 47
一、茄冬雌花初抽物之活性試驗 47
二、茄冬雌花80%酒精水溶液抽取物之分層萃取與成分分離 48
第三章 結果與討論 58
第一節 茄冬雌花初抽物體外活性試驗之評估 58
一、茄冬雌花初抽物之萃取率 58
二、茄冬雌花總酚類化合物含量測定實驗 59
三、茄冬雌花總黃酮類化合物含量測定實驗 62
四、茄冬雌花清除DPPH自由基測定實驗 65
第二節 茄冬雌花與雄花之活性比較 69
一、茄冬雌花與雄花總酚類化合物含量比較 69
二、茄冬雌花與雄花總黃酮類化合物含量比較 70
三、茄冬雌花初抽物之清除DPPH自由基測定實驗 71
第三節 茄冬雌花80%初抽物成分分離之研究 73
一、Compound A (Gallic acid) 75
二、Compound B (Isocoriligen) 78
三、Compound C (Rutin) 91
四、Compound D (Naringenin) 101
第四節 光譜數據 111
第四章 結論 115
參考文獻 116
圖表索引 123
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1Department of Chemistry, Section of Organic Chemistry and Biochemistry, 45110, Ioannina, Greece, 2Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece (2012). Electronic Supplementary Material (ESI) for Organic & Biomolecular Chemistry This journal is ©The Royal Society of Chemistry
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