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研究生:王富銘
研究生(外文):Fu-Ming Wang
論文名稱:穿心蓮及刺五加醇萃物複方對高血脂倉鼠血脂調降之影響
論文名稱(外文):The antihyperlipidemic effect on the combination of ethanol extracts of Andrographis paniculata and Acanthopanax Senticosus in hyperlipidemic hamsters
指導教授:劉崇喜劉崇喜引用關係
學位類別:碩士
校院名稱:大仁科技大學
系所名稱:製藥科技研究所
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:77
中文關鍵詞:穿心蓮刺五加血脂調降
外文關鍵詞:Andrographis paniculataAcanthopanax Senticosusantihyperlipidemic
相關次數:
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本研究將台灣產穿心蓮搭配刺五加以不同比例組成的產品進行降血脂功效評估。本實驗分為兩部分,其一為體外抗氧化試驗,不同比例(1:0、
0:1、1:1、1:2、1:3)穿心蓮與刺五加95%酒精萃取物的組合複方,利用清
除自由基 α,α-diphenyl-b-picrylhydrazyl 分析及微脂粒氧化抑制作用分析其抗氧化效用評估。結果顯示穿心蓮與刺五加 1:3 酒精萃取物的組合複
方具有最佳的清除DPPH 自由基的效果及微脂粒氧化抑制作用,其清除
率為87.3 ± 2.6%,微脂粒氧化抑制率為85.2 ± 2.3%。另試驗第二部份為
動物試驗效用評估,將高膽固醇血症倉鼠 (hyperlipidemic hamster) 餵食不同劑量 (100、300、500 mg/kg/day)穿心蓮與刺五加1:3 組合複方,8週後進行實驗動物血脂變化的數據評估,並以與餵食壽美降脂一號 (100
mg/kg/day) 的實驗動物所得結果相對照。試驗結果發現,給予穿心蓮與
刺五加1:3 組合複方 (500 mg/kg/day) 8 週後的高膽固醇血症倉鼠其血脂
質總膽固醇 (TC) 、三酸甘油酯 (TG) 、低密度脂蛋白膽固醇 (LDL-C)
可分別調降24.34 ± 2.21%、22.94 ± 2.97%與22.68 ± 2.76%; 而餵食壽美降脂一號之高膽固醇血症倉鼠其血脂質總膽固醇、三酸甘油酯、低密度
脂蛋白膽固醇則分別調降27.22±3.14%、31.06±2.83%與26.63±3.12%。
由結果可知穿心蓮與刺五加以1:3 比例組成的複方應與壽美降脂一號具
有相似調降血脂的功效。
The aim of the present study was going to evaluate the plasma lipids lowering effect on the prescription composed of Andrographis paniculata extract and Acanthopanax Senticosus extract at the ratio of 1:3 (APAS). In the
present study, the plasma lipid levels were evaluated after hyperlipidemic hamster receiving 8-weeks oral administration with APAS, that was compared with those from LipoCol Forte○R (100 mg/kg/day)-treated group. It has been shown that the plasma lowering activities of APAS (500 mg/kg/day) in TC, TG and LDL-C was approached to 24.34±2.21, 22.94±2.97 and 22.68±2.76%,
respectively. The plasma lowering activities of LipoCol Forte (100 mg/kg/day) in TC, TG and LDL-C was nearly to 27.22±3.14, 31.06±2.83 and 26.63±3.12 %, respectively. The plasma lipids lowering potency of APAS is similar with
that produced by LipoCol Forte. The results showed that APAS poses the capacity to ameliorate hyperlipidemia.
目 錄
中文摘要………………………………………………………………..Ⅰ
英文摘要………………………………………………………………..Ⅱ
誌謝……………………………………………………………………..Ⅲ
目錄……………………………………………………………………..Ⅳ
表目錄……………………………………………………………………..Ⅵ
圖目錄……………………………………………………………………..Ⅶ
第一章 前言……………………………………………………………1
第二章 總論……………………………………………………………3
第一節 刺五加文獻回顧…………………………………………..3
第二節 穿心蓮文獻回顧…………………………………………..10
第三節 壽美降脂一號(LipoCol Forte) ……… .…………...…………12
第四節 高血脂症(Hyperlipidemia)…………………………………13
第三章 材料與方法………………………………………………… ……17
第一節 實驗動物……………………………..………………… ……17
第二節 儀器設備……………………………………..………… ……17
第三節 試劑與藥品………………………………………..…… ……18
第四節 實驗方法……………………………………………… ..……22
(一)、穿心蓮與刺五加混合萃取物的製備………………… …….22
(二)、抗氧化試驗……………………………………………………22
(三)、指標成分分析…………………………………………………23
(四)、動物試驗…………………………………….……………….23
(五)、統計分析…………………………………..…………………26
第四章 結果…………………………………………………………27
V
第五章 討論…………………………………………………………36
第六章 結論…………………………………………………………37
第七章 參考文獻……………………………………………………55
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