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研究生:楊博宇
研究生(外文):Po-Yu Yang
論文名稱:元寶草微體繁殖之研究
論文名稱(外文):Studies on micropropagation of Hypericum sampsonii Hance
指導教授:張筱筠張筱筠引用關係闕甫伈
指導教授(外文):Hsiao-yun ChangFu-shin Chueh
口試委員:張有明張筱筠闕甫伈
口試委員(外文):You-Ming ChangHsiao-yun ChangFu-shin Chueh
口試日期:2012-07-03
學位類別:碩士
校院名稱:亞洲大學
系所名稱:生物科技學系碩士班
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:62
中文關鍵詞:元寶草組織培養
外文關鍵詞:Hypericum sampsonii Hance
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元寶草(Hypericum sampsonii Hance)為金絲桃(Guttiferae)金絲桃屬(Hypericum L.)多年生草本植物,全草入藥,能治療內出血、肝炎、腫瘡毒,並能抗風濕,調經、止血生肌等。該屬植物所含的金絲桃素具有抗憂鬱、抑制中樞神經、抗病毒和增強免疫等功能。近年來藥理研究發現,金絲桃素具有顯著的抗DNA、RNA病毒作用,可用於愛滋病的治療,引起醫藥界對此屬植物的高度重視,是頗具開發價值的中草藥。
本研究目的,是要探討元寶草莖段培殖體培養於不同植物生長素和細胞分裂素對元寶草芽體誘導及生長的影響,進而建立植株之大量繁殖的系統。結果顯示,將元寶草莖段培養含於0.2mg/l BA之1/2MS培養基中能誘導約6.7個不定芽。將不定芽繼代至含0.1mg/l IBA及5%蔗糖之1/2MS培養基中能得到最健全的根系,平均根長約1.93 cm,根數也達9條以上。
本研究利用微體培養技術建立元寶草繁殖途徑,期能經由元寶草之提供大量植株,解決元寶草生態保育問題及產業發展,對藥用植物的開發有所助益。

Hypericum sampsonii Hance,is a species of Hypericum,Guttifera genera perennial herb, whole plant medicine to treat internal bleeding, hepatitis, swollen and sore, and anti-rheumatic, menstruation, hemostasis Healthmuscle and so on. The genus contains hypericin antidepressants, inhibit the central nervous system, anti-virus and enhanced immune function. In recent years, pharmacological studies have found that hypericin has a significant anti-DNA, RNA effects of the virus can be used for the treatment of AIDS, caused by the pharmaceutical industry attaches great importance to this species is quite value for development of Chinese herbal medicine.
The purpose of this study was to investigate the ingot stem segment explants were cultured in different auxin and cytokinin sampsonii buds induction and growth, thereby establishing the plant breeding system. The results showed that the ingot stem segment culture containing 0.2mg / l BA 1/2MS medium induced 6.7 adventitious buds. Adventitious buds can get the most sound of the root subculture to 1/2MS medium containing 0.1mg / l IBA and 5% sucrose, the average root length of 1.93 cm, the root of the number of 9.
In this study, micro-cultured establish H. sampsonii propagation was to provide a large number of plants of the can through H. sampsonii to solve the ecological conservation and industrial development of H. sampsonii be helpful to the development of medicinal plants.

圖目錄.............................................................................iv
表目錄.............................................................................iii
中文摘要............................................................................v
英文摘要............................................................................vi
略字解.............................................................................vii
第一章 前言........................................................................1
第二章 文獻回顧.....................................................................3
第一節 金絲桃之植物學考察..............................................................3
一.金絲桃屬(Guttiferae)植物共同特...............................................3
二.台灣金絲桃屬植物之形態...........................................................5
第二節 元寶草之藥用植物學考察...........................................................13
一.別名......................................................................13
二.植物形態...................................................................13
三.生境分布...................................................................14
伍.元寶草成分.................................................................14
六.元寶草藥理作用..............................................................15
七.性狀......................................................................16
第三章 植物組織培養...................................................................17
一、培植體之選擇................................................................18
二、培養基之組成................................................................19
三、培養方式...................................................................24
四、培養環境...................................................................25
第四章 材料與方法......................................................................28
第五章 結果...........................................................................31
第六章 討論...........................................................................44
結論.................................................................................46
參考文獻..............................................................................54
附錄.................................................................................56



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1. 楊淑如,戴宗德,陳忠川,蔡新聲 植物生長調節劑及培養基型態對湖北貝母鱗片培養衍生芽球相似體及小鱗片增重之影響。中華農學會報。2001b;2:414-22。
2. 楊淑如、蕭翌柱、陳威臣、夏奇鈮 凝膠物質的理化特性與其在植物組織培養上之應用(一)凝膠物質的種類與其理化特性。科學農業。2004a;52:p236-43。
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