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研究生:劉中瑞
研究生(外文):ZHONG-RUI LIU
論文名稱:膠原蛋白/透明質酸/三鈣磷酸鹽複合醫材之研究
論文名稱(外文):Study of collagen/hyaluronic acid/tricalcium phosphate composites
指導教授:鍾仁傑鍾仁傑引用關係
口試委員:游佳欣許富銀方旭偉
口試日期:2012-01-17
學位類別:碩士
校院名稱:國立臺北科技大學
系所名稱:化學工程研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:73
中文關鍵詞:複合填補材料第一型膠原蛋白低聚合原花青素透明質酸三鈣磷酸鹽細胞實驗
外文關鍵詞:Composite Restoration MaterialType I CollagenOligomeric ProanthocyanidinsHyaluronic AcidTricalcium PhosphateIn vitro Study
相關次數:
  • 被引用被引用:3
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本實驗利用原花青素為天然交聯劑,以製備膠原蛋白、透明質酸、三鈣磷酸鹽複合式填補材料 (CHTP)。在材料物性分析部分,首先以不同交聯程度的膠原蛋白材料透過固定指數進行交聯程度評估,之後以最適化交聯程度的 CHTP 複合材料,利用機械強度、含水率以及生物降解速率測定來評估其材料特性。生物相容性部分則以類骨母細胞 ( MG-63) 細胞進行體外測試。
經 5% 原花青素交聯後的複合材料 (CHTP-5%) 具有很高的澎潤度 (420%) 與機械強度 (40 kPa),經三十天之體外降解測試後仍有60%以上餘重。體外生物適應性結果亦顯示,CHTP-5% 複合材料對於 MG-63 細胞具有促進增生之效用。由材料機械性質、澎潤度、降解速率以及體外生物適應性等實驗結果顯示, CHTP-5% 複合材料為一具潛力的填補材料。


In this study, composite restoration materials (CHTP) made of type I collagen, hyaluronic acid (HA) and tricalcium phosphate (β-TCP) were prepared and crosslinked with the natural crosslinking agent, oligomeric proanthocyanidins (OPCs). The crosslinking property was evaluated. The mechanical strength, swelling ratio and in vitro degradation rate of the optimally crosslinked CHTP composite were investigated. In vitro tests were carried out to evaluate the biocompatibility using MG-63 cell line.
Results indicated that the crosslinked composite using 5% OPCs solution (CHTP-5%) had high swelling ratio (420%) and mechanical strength (40 kPa), and remained more than 60% weight after 30 days in vitro degradation test. The results of in vitro tests showed that CHTP-5% would promote the proliferation of MG-63 cell. The results of material analyses and in-vitro tests showed that CHTP-5% composite is of great potential to serve as a useful bone restoration material.


摘要 i
Abstract ii
致謝 iv
目錄 v
表目錄 ix
圖目錄 x
第一章 文獻回顧 1
1-1 前言 1
1-2 齒槽骨重建技術 2
1-3 研究動機與目的 3
1-4 研究流程與章節說明 4
第二章 文獻回顧 5
2-1 生醫材料 5
2-1-1 可降解性植入材料 5
2-1-2 人工支架所需具備的特性 5
2-2 膠原蛋白的基本介紹 6
2-2-1 膠原蛋白分子結構與化學組成 6
2-2-2 膠原蛋白的免疫排斥性與物理特性 7
2-2-3 膠原蛋白於醫學上的應用 8
2-3 透明質酸 8
2-3-1 透明質酸簡介 8
2-3-2 透明質酸的生理功能 9
2-3-3 透明質酸的來源 12
2-3-4 透明質酸的修飾 12
2-4 三鈣磷酸鹽 14
2-5 低聚合原花青素 14
2-6 四環黴素 17
2-7 生物評估方法與原理 18
2-7-1 細胞生物相容性評估 18
第三章 實驗方法 20
3-1 實驗藥品與儀器 20
3-1-1 實驗藥品 20
3-1-2 實驗儀器 22
3-1-3 簡寫符號表 22
3-2 實驗流程 23
3-3 實驗步驟 24
3-3-1 膠原蛋白去免疫端之前處理 24
3-3-2 氧化透明質酸之製備 (oHA) 25
3-3-3 膠原蛋白/氧化透明質酸複合材料(CH-10~40%) 25
3-3-4 原花青素交聯膠原蛋白/氧化透明質酸複合材料(CHP-3%、CHP5%) 25
3-3-5 膠原蛋白/氧化透明質酸/三鈣磷酸鹽複合材料 (CHT-10~40%) 25
3-3-6 原花青素交聯膠原蛋白/氧化透明質酸/三鈣磷酸鹽複合材料(CHTP-3%、 CHTP-3%) 26
3-4 材料特性分析 27
3-4-1 傅立葉轉換紅外線光譜儀 (fourier transform infrared spectrometer) 27
3-4-2 交聯指數分析 (crosslinking index) 27
3-4-3 膨潤度測試 (swelling ratio) 30
3-4-4 降解度測試 (degradation) 31
3-4-5 機械強度測試 (mechanical tet) 33
3-4-6 掃描式電子顯微鏡 (scanning electron microscope,SEM) 36
3-4-7 藥物制放測試 37
3-5 生物適應性評估 38
3-5-1 類骨母細胞培養測試 38
第四章 結果與討論 41
4-1 CH 複合材料之材料分析評估 41
4-1-1 光譜分析 41
4-1-2 掃描式電子顯微鏡觀察 43
4-1-3 交聯指數測試 45
4-1-4 膨潤度測試 46
4-1-5 機械性質測試 (拉伸) 47
4-1-6 降解度測試 48
4-1-7 細胞毒性測試 49
4-2 CHP 複合材料之材料分析評估 52
4-2-1 膨潤度測試 52
4-2-2 機械性質測試 (拉伸) 53
4-2-3 降解度測試 53
4-2-4 細胞毒性測試 54
4-3 CHT 複合材料之材料分析評估 57
4-3-1 膨潤度測試 57
4-3-2 機械性質測試 (抗壓強度) 58
4-3-3 降解度測試 59
4-3-4 細胞毒性測試 60
4-4 CHTP 複合材料之材料分析評估 61
4-4-1 膨潤度測試 61
4-4-2 機械性質測試 (抗壓強度) 62
4-4-3 降解度測試 63
4-4-4 細胞毒性測試 64
4-4-5藥物制放測試 64
第五章 結論 66
參考文獻 68



[1]Heitz-Mayfield, L.J.A., Schatzle, M., Loe, H., Burgin, W., Anerud, A., Boysen, H., Lang, N.P. Clinical course of chronic periodontitis. II. Incidence, characteristics and time of occurrence of the initial periodontal lesion. Journal of Clinical Periodontology 2003;30:887-901.
[2]Renvert, S., Persson, G.R. Patient-based assessments of clinical periodontal conditions in relation to alveolar bone loss. Joumal of Clinical Periodontology 2004;31:208-213.
[3]詹進忠,可注射式聚乳酸/幾丁聚醣/非晶型磷酸鈣牙周再生阻隔材之活體評估,私立台北醫學大學口腔醫學院牙醫學系研究所碩士論文,2006。
[4]Ono, K., Yamamuro, T., Nakamura, T., Kakutani, Y., Kitsugi, T., Hyakuna, K., Kokubo T., Oka M., Kotoura Y. Apatite-wollastonite containing glass ceramic-fibrin mixture as a bone defect filler. Journal of Biomedical Materials Research 1988;22:69-85.
[5]Yao, C.H., Sun, F.H., Lin, C.J., Huang, C.W. Biological effects and cytotoxicity of tricalcium phosphate and formaldehyde crosslinked gelatin composite. Materials Chemistry and Physics 1996;45:6-14.
[6]陳翊綾,骨組織工程來修補牙周及齒槽骨缺陷,私立長庚大學顱顏口腔醫學研究所碩士論文,2004。
[7]Kim, S., Marcel E., Nimni, Yang, Z., Han, B. Chitosan/gelatin–based films crosslinked by proanthocyanidin. Journal of Biomedical Materials Research - Part B Applied Biomaterials 2005;75:442-450.
[8]柯智升,利用多孔性 Collagen II/chondroitin sulfate/hyaluronic acid 載體誘導間葉幹細胞應用於組織工程軟骨之研究,國立清華大學化學工程研究所博士論文,2008。
[9]劉百栓,以天然交聯劑綠梔子素交聯明膠結合三鈣磷酸鹽粉末之新骨科替代材料的研製與評估,國立國立中興大學化學工程研究所博士論文,2004。
[10]Lubert S. Biochemistry, Freeman, New York, 1988.
[11]曾國輝,大學生物化學,藝軒書局,1993。
[12]Nerem, R.M. Tissue engineering in the USA. Medical and Biological Engineering and Computing 1992;30:8-12.
[13]Wang, M.C., Pins, G.D., Silver, F.H. Collagen fibres with improved strength for the repair of soft tissue injuries. Biomaterials 1994;15:507-512.
[14]Tompkins, R.G., J.F. Burke. Progress in burn treatment and the use of artificial skin. World Journal of Surgery 1990;14:819-824.
[15]Laurent, T.C., Fraser, J.R.. Hyaluronan. The FASEB Journal 1992;6:2397-2404.
[16]Singla, S.K. Nomenclature of hyaluronic acid. Biochemical Journal 1987;242:623.
[17]Laurent, T.C. Biochemistry of hyaluronan. Acta Oto-Laryngologica 1987;104:7-24.
[18]Yoneda, M. Hyaluronic acid-dependent changes in the extracellular matrix of mouse dermal fibroblasts that is conductive to cell proliferation. Journal of Cell Science 1988;90:275-286.
[19]Hakkanson, L. The combined action of hyaluronic acid and fibronectin stimulates neutrophil migration. Journal of Immunology 1985;135:2735-2739.
[20]Toole, B.P. The extracellular matrix of the regenerating newt limb: synthesis and removal of hyaluronate prior to differentiation. Developmental Biology 1971;25:57-77.
[21]王正一主編,醫學工程原理與應用,正中書局,1996。
[22]Meyer, K., Palme, J.W. The polysaccharide of the vitreonshmor. Journal of Biological Chemistry 1934;107:629-634.
[23]黃定國,透明質酸之開發與應用,菌種保存及研究簡訊,2001。
[24]Balazs, E.A. Medical applications of hyaluronan and its derivatives. Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering 1990;63:689-691.
[25]Toole, B.P. Hyaluronan: from extracellular glue to pericellular cue. Nature Reviews Cancer 2004;4:528-539.
[26]Fraser, J.R. Hyaluronan: its nature, distribution, functions and turnover. Journal of Internal Medicine 1997;242:27-33.
[27]Chen, W.Y.J., Abatangelo, G. Functions OF hyaluronan in wound repair. Wound Repair and Regeneration 1999;7:79-89.
[28]Samuels, P., Tan, A.K.W. Fetal scarless wound healing. Journal of Otolaryngology 1999;28:296-302.
[29]Renvert, S., Persson, G.R. Patient-based assessments of clinical periodontal conditions in relation to alveolar bone loss. Journal of Clinical Periodontology 2004;31:208-213.
[30]Underhill, C. CD44: the hyaluronan receptor. Journal of Cell Science 1992;103:293-298. Chvapil M, Speer DP, Holubec H, Chvapil TA, King DH. Collagen fiber as a temporary scaffold for replacement of A
[31]Gustafson, S., Wikstrom, T. Histochemical studies of hyaluronan and the hyaluronan receptor ICAM-1 in psoriasis. International Journal of Tissue Reactions 1995;17:167-173.
[32]Hall, C.L., Turley, E.A. Hyaluronan: RHAMM mediated cell locomotion and signaling in tumorigenesis. Journal of Neuro-Oncology 1995;26:221-229.
[33]Assmann, V. The intracellular hyaluronan receptor RHAMM/IHABP interacts with microtubules and actin filaments. Journal of Cell Science 1999;112:3943-3954.
[34]Zhang, S. The hyaluronan receptor RHAMM regulates. Journal of Biological Chemistry 1998;273:11342-11348.
[35]Abetamann, V., Kern, H.F., Elsasser, H.P. Differential expression of the hyaluronan receptors CD44 and RHAMM in human pancreatic cancer cells. Clinical Cancer Research 1992;6:1607-1618.
[36]Setala, L.P. Hyaluronan expression in gastric cancer cells is associated with local and nodal spread and reduced survival rate. British Journal of Cancer 1999;79:1133-1138.
[37]Kayastha, S. Expression of the hyaluronan receptor, CD44S, in epithelial ovarian cancer is an independent predictor of survival. Clinical Cancer Research 1999;5:1073-1076.
[38]Matsubara, Y. Expression of CD44 variants in lung cancer and its relationship to hyaluronan binding. Journal of International Medical Research 2000;28:78-90.
[39]http://www.glycoforum.gr.jp/science/hyaluronan/ hyaluronanE.html.
[40]Brynin, R. Soy and its isoflavones: a review of their effects on bone density. Alternative Medicine Review 2002;7:317-322.
[41]Clifford, M.N. Anthocyanins-nature, occurrence and dietary burden. Journal of the Science of Food and Agriculture 2000;80:1063-1072.
[42]Gabetta, B. Characterization of proanthocyanidins from grape seeds. Fitoterapia 2000;71:162-175.
[43]李鳳英,葡萄皮籽多分物質的提取技術研究,中國農業大學碩士論文,2004。
[44]何包,葡萄籽中原花青素提取研究,浙江大學碩士論文,2004。
[45]林峰輝,醫學工程教科書大綱(5)--組織工程,王正一主編,教育部醫學工程科技教科書,2000。
[46]Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 1983;65:55-63.
[47]Chang, S.J., Kuo, S.M., Liu, W.T., Niu C.C.G. Gellan Gum Films for Effective Guided Bone Regeneration. Journal of Medical and Biological Engineering 2010;30:99-103.
[48]Peraira, C.A., Lee, J.M., Haberer, S.A. Effect of alternative crosslinking methods on the low strain rate viscoelastic properties of bovine pericardial bioprosthetic material. Journal of Biomedical Materials Research 1990;24:345-361.
[49]Skopinska-Wisniewska, J., Sionkowska, A,, Kaminska, A., Kaznica, A., Jachimiak, R., Drewa, T. Surface characterization of collagen/elastin based biomaterials for tissue regeneration. Applied Surface Science 2009;255:8286-8289.
[50]Dellon, A.L., Mackinnon, S.E. Selection of the appropriate parameter to measure neural regeneration. Annals of Plastic Surgery 1989;23:197-202.
[51]Van Dyke, T.E., Lester, M.A., Shapira, L. The role of the host response in periodontal disease progression: implications for future treatment strategies. Journal of Periodontology 1993;64:792-806.
[52]Greenstein, G., Polson, A. The role of local drug delivery in the management of periodontal diseases: A comprehensive review. Journal of Periodontology 1998;69:507-520.
[53]Van Winkelhoff, A.J., Rams, T.E., Slots, J. Systemic antibiotic therapy in periodontics. Periodontology 2000 1996;10:45-78.
[54]Rifkin, B.R., Vernillo, A.T., Golub, L.M. Blocking periodontal disease progression by inhibiting tissue-destructive enzymes: a potential therapeutic role for tetracyclines and their chemically-modified analogsic. Journal of Periodontology 1993;64:819-827.
[55]Seymur, R.A., Heasman, P.A. Tetracyclines in the management of periodontal diseases. A review. Journal of Clinical Periodontology 1993;22:22-35.



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