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研究生:黃慧婷
研究生(外文):Huei-Ting Huang
論文名稱:關節液生物分子對人工關節表面摩擦行為之影響
論文名稱(外文):Effects of Synovial Biomolecules on Tribological Behaviors of Arthroplastic Surface
指導教授:方旭偉方旭偉引用關係
指導教授(外文):Hsu-Wei Fang
口試委員:吳彰哲林子超張印本楊長彬段葉芳
口試日期:2012-07-24
學位類別:博士
校院名稱:國立臺北科技大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:英文
論文頁數:102
中文關鍵詞:人工關節關節液摩擦係數白蛋白吸附
外文關鍵詞:Artificial jointsynovial fluidfriction coefficientalbuminadsorption
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超高分子量聚乙烯(UHMWPE)與鈷鉻鉬合金(CoCrMo alloy)組合是最常見的人工關節對磨組合。UHMWPE所產生的磨耗顆粒會引起人體的免疫反應,進而引發骨溶解現象,最後造成人工關節鬆脫。人工關節於關節液中的摩擦潤滑性質是影響其使用年限的關鍵。關節液中的主要成份包括: 白蛋白、球蛋白、玻尿酸和脂質等,不同的成份扮演不同的摩擦特性。因此,基礎性的了解關節液中每個成份的潤滑特性是必須的,尤以所占含量最多的白蛋白為首。
本研究利用pin-on-disc摩擦以及quartz crystal microbalance (QCM) 吸附等實驗,研究白蛋白於UHMWPE和 CoCrMo alloy 材料間之摩擦行為。實驗結果得知,於邊界潤滑運行條件下,白蛋白扮演潤滑抑制的角色,濃度越高產生的摩擦阻力越大。本研究同時探討白蛋白構型變化對其在人工關節材料表面吸附行為的影響,並了解吸附與摩擦間的關連性。實驗觀察得知,在互相對磨的兩介面間,白蛋白的吸附量與摩擦係數具有正相關性。且二級結構損失會有較高的吸附量或吸附速率,進而形成一層較緊密厚實的吸附層於關節材料表上,使得對表面間的摩擦阻力增加。此外,我們更深入一步探討玻尿酸(HA)對白蛋白摩擦潤滑行為之影響。結果顯示,在白蛋白及玻尿酸混合溶液中,玻尿酸能有效降低摩擦係數。經實驗設計及驗證後,我們針對玻尿酸影響白蛋白摩擦潤滑行為提出三種可能的模式,包括: 減震模式(damping mode)、驅逐模式(expelling mode)以及抗形變模式(anti-deformation),不同的作用模式會有不同的影響機制。本研究探討關節液中生物分子對人工關節的潤滑行為。藉由基礎性的了解潤滑機制,或許可協助開發出有效的人工關節潤滑添加劑,減少元件磨損,以延長人工關節的使用壽命。


Ultra-high molecular weight polyethylene (UHMWPE) sliding against CoCrMo alloy is common in total joint implant. Wear of UHMWPE and wear debris induced osteolysis and bone resorption which lead to the artificial joint loosening and failure. The lifetime of artificial joint is mainly determined by their biotribological properties in synovial fluid. Various compositions such as albumin, globulin, hyaluronic acid and phospholipid are contained in the synovial fluid. Each component of synovial fluid shows different frictional behaviors. Therefore, it is necessary to fundamentally understand the lubrication behaviors of the various biological molecules in the synovial fluid, especially, the most abundant composition-albumin (HSA).
In this study, the pin-on-disc friction tests and quartz crystal microbalance (QCM) adsorption experiments were carried out to investigate the albumin-mediated tribological processes under the articulation of UHMWPE and CoCrMo alloy materials. We concluded that albumin acts as a lubrication inhibitor in boundary lubrication regime and as the albumin concentration increased, the coefficient of friction also increased. The influence of albumin conformation on the adsorption behaviors on the articulating surfaces and the relationship between adsorbed albumin and its tribological behaviors were also studied. It was observed that loss of secondary structure of albumin lead to a higher adsorption rate onto the articulating material surface and formed a compact layer of protein, which results in the increase of friction coefficient. Moreover, we further studied the effects from addition of hyaluronic acid (HA) molecules on the albumin-mediated tribological processes. The results indicated that the elevation of HA caused a decrease of the frictions under HSA-HA mixed lubrication conditions by three modes, including damping, expelling and anti-deformation mode. Different modes have different mechanisms that affect friction behavior of albumin in boundary lubrication condition. In this study, we attempted to investigate the role of different biological compositions of synovial fluid on the lubrication of the artificial joint. By understanding the lubricating mechanism, it is possible to find more effective lubricating additives to further decrease the wear and prolong the life of artificial joints.


摘要 i
ABSTRACT iii
ACNOWLEDGEMENT v
ABBREVIATION xiii
Chapter 1 Introduction 1
Chapter 2 Background 3
2.1 Need to replace human joints 4
2.2 Total joint arthroplasty 5
2.2.1 Components of total joint prostheses 6
2.2.2 UHMWPE 6
2.3 Failure of total joint arthroplasty 8
2.3.1 UHMWPE wear particles generation 9
2.3.2 UHMWPE wear particles induced aseptic loosening 11
2.4 Tribology 14
2.4.1 What is Tribology? 14
2.4.2 Lubrication regimens 15
2.5 Biotribology in the artificial joint 17
2.5.1 Synovial fluid 17
2.5.2 Friction study of artificial joint 19
Chapter 3 Problem definition and study methods 24
Chapter 4 Material and methods 26
4.1 Friction coefficient measurement 27
4.2 Quantification of composition for synovial fluid 30
4.2.1 ELISA Analysis of albumin and IgG 30
4.2.2 Analysis of phospholipids 32
4.2.3 Analysis of hyaluronic acid 32
4.3.1 Measurement of adsorption by QCM 34
4.4 Conformational analysis of protein 36
4.4.1 Measurement of secondary structure by CD spectrum 36
4.4.2 Measurement of peptide band by SDS-PAGE 37
4.5.1 Mechanical properties measurement by nanoidentation 38
Chapter 5 Tribological Behavior of Albumin in Boundary Lubrication Regime 41
5.1 Purpose 41
5.2 Friction behaviors of simulated synovial fluid 42
5.4.1 Friction behaviors of Albumin of Different Concentrations 50
5.4.2 Friction behaviors of Albumin of under different normal loads 51
5.4.3 Adsorption behaviors of albumin 52
5.5 Boundary lubrication model of albumin 54
Chapter 6 Conformation and Adsorption of Albumin Affect boundary lubrication 55
6.1 Purpose 55
6.2 Method 55
6.3 Conformational change of albumin 58
6.4 Tribology behavior of albumin 60
6.5 Adsorption characteristics of albumin 62
6.7 Protein conformational effect on friction 65
Chapter 7 Lubricating characteristics of HA on the albumin-mediated tribological processe 67
7.1 Purpose 67
7.2 Method 68
7.3.1 Friction coefficients with various concentrations of HSA and HSA-HA mixed solutions 69
7.3.2 Friction coefficients for thermal processed of DHSA and DHSA-HA mixed solutions 71
7.3.3 Adsorption experiments 73
7.3.4 Mode of HA on albumin-mediated boundary lubrication 74
7.4.1 Conformational change of albumin 79
Chapter 8 Conclusion 83
Reference 86
Appendix A Publication and Research Experience 91
Appendix B Development assessment of medical grade lubricating additives for arthroplasty 97


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